CAZyme Information: MGYG000004348_00735

Basic Information

• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella
• CAZyme ID: MGYG000004348_00735
• CAZy Family: CE2
• CAZyme Description: Cellulase/esterase CelE

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
362	MGYG000004348_3|CGC2	40194.81	6.4074

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004348	1551350	MAG	Israel	Asia

• Gene Location: Start: 163367; End: 164455 Strand: +

Full Sequence      

MLSVKCVIFAVLACVPMLCGAQAPASAFVPADDPNIVYMGRTSWRTPGCVSFTYPGVSIF
ADFEGTSLQMKAKPGSGDFMVEIDGGLPFRIHFGEKDSVMTLVEGLPDGTHSARLMYAVE
GHGFRPEFRGFYLDGGKKLAPAPQLPRRRIEFIGNSITCGYGIESEDRDAPFTYETENHY
YTYAALTARALEAQHLVVARSGIGVYRNYGSPASGSPDCMPAMYEQTLFLDSTEQWNHDL
YTPDVVCVNLGTNDVSEDKYDEKLLTDAYRGFAAHLRGIYPKSKIVMLTGCMLNGRQLDA
VKKALDTVTAERRAKGDDEVYRFDFTPQTGSLGYAPGWHPTMRQHRKMAVELTTYLKKLM
NW

Enzyme Prediction     

No EC number prediction in MGYG000004348_00735.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
CE2	149	356	5.8e-65	0.9952153110047847

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd01831	Endoglucanase_E_like	4.30e-42	149	357	1	169	Endoglucanase E-like members of the SGNH hydrolase family; Endoglucanase E catalyzes the endohydrolysis of 1,4-beta-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
pfam17996	CE2_N	1.19e-33	38	143	1	108	Carbohydrate esterase 2 N-terminal. This is the N-terminal beta-sheet domain with jelly roll topology found in CE2 acetyl-esterase from the bacterium Clostridium thermocellum. This enzyme displays dual activities, it catalyses the deacetylation of plant polysaccharides and also potentiates the activity of its appended cellulase catalytic module through its noncatalytic cellulose binding function. This N-terminal jelly-roll domain appears to extend the substrate/cellulose binding cleft of the catalytic domain in C.thermocellum.
pfam13472	Lipase_GDSL_2	5.42e-10	152	347	1	176	GDSL-like Lipase/Acylhydrolase family. This family of presumed lipases and related enzymes are similar to pfam00657.
cd00229	SGNH_hydrolase	2.13e-09	150	355	1	187	SGNH_hydrolase, or GDSL_hydrolase, is a diverse family of lipases and esterases. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases; its active site closely resembles the typical Ser-His-Asp(Glu) triad from other serine hydrolases, but may lack the carboxlic acid.
cd01827	sialate_O-acetylesterase_like1	2.44e-06	149	349	2	180	sialate O-acetylesterase_like family of the SGNH hydrolases, a diverse family of lipases and esterases. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases; its active site closely resembles the Ser-His-Asp(Glu) triad found in other serine hydrolases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADY36201.1	1.44e-184	14	362	21	369
ADE83543.1	5.31e-116	9	362	9	375
QNT66876.1	6.38e-116	29	362	36	370
QVJ79798.1	1.07e-115	9	362	9	375
QCD41872.1	2.81e-114	17	360	19	359

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2WAO_A	1.69e-57	32	362	7	333	ChainA, ENDOGLUCANASE E [Acetivibrio thermocellus]
2WAB_A	4.70e-57	32	362	7	333	ChainA, ENDOGLUCANASE E [Acetivibrio thermocellus]
2WAA_A	1.23e-37	29	362	13	339	Structureof a family two carbohydrate esterase from Cellvibrio japonicus [Cellvibrio japonicus]
2W9X_A	9.88e-26	50	358	46	355	Theactive site of a carbohydrate esterase displays divergent catalytic and non-catalytic binding functions [Cellvibrio japonicus],2W9X_B The active site of a carbohydrate esterase displays divergent catalytic and non-catalytic binding functions [Cellvibrio japonicus]
4XVH_A	1.40e-24	35	362	1	329	Crystalstructure of a Corynascus thermopiles (Myceliophthora fergusii) carbohydrate esterase family 2 (CE2) enzyme plus carbohydrate binding domain (CBD) [Chaetomium]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P10477	2.92e-53	32	362	488	814	Cellulase/esterase CelE OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=celE PE=1 SV=2
B3PIB0	8.46e-37	29	362	32	358	Acetylxylan esterase / glucomannan deacetylase OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=axe2C PE=1 SV=1
B3PDE5	4.97e-25	50	358	46	355	Acetylxylan esterase / glucomannan deacetylase OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=ce2C PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000314	0.998918	0.000291	0.000178	0.000140	0.000131

TMHMM  Annotations     

start	end
7	29