CAZyme Information: MGYG000004786_00711

Basic Information

• Species: Firm-11 sp900550955
• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; CAG-74; Firm-11; Firm-11 sp900550955
• CAZyme ID: MGYG000004786_00711
• CAZy Family: GT39
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1371	MGYG000004786_9|CGC1	151497.32	7.1183

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000004786	2686597	MAG	Denmark	Europe

• Gene Location: Start: 29188; End: 33303 Strand: +

Full Sequence      

MKRFWAALCAALLLLLGAAATAETAETADNLLSNGGFELLNAAGEPVDWYASAYRTQAGY
SRISVTDEKARSGKYSAMVENATANDARYTCTVPVMPNSLYRLSGYVLVDAMNDAGNGAN
FAVEGIYAFSECLFDTEGEWRYLEWYGETGENQTELTFGVRVGGYGAESVGRAYFDDIVL
EKVDSLPQEIIASVWYAVKAEPAAASAAAESNGEKSTTLFIMIAVVFGLLYLCCRPILRS
SSEKPALICFAVLMLLAVLARVLLAVRVPGYSVDINCFTAWSLRMAENGPSAFYHADYFC
DYPPGYLLLLWPCGLLLRAVGLADTQAALLIVKSLPLLCDILGSLWLFRFSKKRIGTAAA
ALASLLYALNPAVLVNGAAWGQADSVLALMLALTACFAMGRRWHFAMPFFAASVLIKPQA
LLFAPVGGVWLLLSLAAKQDKAERRHQAKLLGVGVLAAAAVAAIIILPFNGQQEPGWLVR
LYRDTLGSYAYATVNAANLYYLLGANWAGLETLISPVLAGATAVVFGCMGALGYLGKRFT
LVAAVPWPKRRALSALMMAEALAQLVLAILPATYNLYGYALMAFAFLFAILCMAYDRRPE
CLPFFMALSLLMIFVLGIKVHERYLFPAILLLLMGYALTRDRRLLALCVGFSMTTFVNTA
IVLENSILFGAEKGHLNADTQAINILLCILNLCLCLYGLYVGWTDLRETKVEESAPEAFV
PASYEAMLFHPSDARLHLSLRDYVIMGITTLLYAILAFTNLGSTVAPQTGWVSTSADEKV
VFDLGKQETFSLLYYAGVSYYDFSVSVSDDGVNWSEETPLQMREGLCYRWLYAVNAQTVD
GKVAYADAAPQNIKWFTGRYLRLNAEDAGLNLWEIVARNEAGETLPLTISGHTGANESIL
KEPRPVENLIDEQDTCVGEPSWYNGTYFDEIYHARTGYEHLHGIKPYETTHPPLGKLMMS
VGIAIFGMTPFGWRFAGTLIGVLMLPALYLLAMQLTHKRGIATLSMAAFSLDLMHYTQTR
IATIDSFPVFFILLSYLFMVRYMQTDVFATCEGAAPRLFDKPFRKSLVPLLFSGVFMGLG
IASKWIGIYSAVGLAVLFFIAIYRQYRVSNVACGLNAAGGGRTKQEEARILCAQDLTLKR
ILITCGFCVLFFILIPCVIYYLCYIPYLLPTGPVTLKRVISAQQSMLDYHSTPGLGMDHF
FQSPWWQWPFILKAMWFACDSYEPAGYASTIMCMGNPWVFYLGAVAMLGVLFACAAKYVR
IRERVTLKQGDGDLTLPVLVIGFLAQYLPWMLVPRSMYIYHYFASVPFIILATAWWIGRI
PKARPKWRHGVMAAYLIGAAVFFVMFFPYASGWLTTTNWLNALKWFPGLYY

Enzyme Prediction     

No EC number prediction in MGYG000004786_00711.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT39	924	1167	2.7e-52	0.9147982062780269

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam16192	PMT_4TMC	1.48e-22	1177	1367	1	198	C-terminal four TMM region of protein-O-mannosyltransferase. PMT_4TMC is the C-terminal four membrane-pass region of protein-O-mannosyltransferases and similar enzymes.
COG1928	PMT1	9.91e-18	928	1164	50	264	Dolichyl-phosphate-mannose--protein O-mannosyl transferase [Posttranslational modification, protein turnover, chaperones].
pfam02366	PMT	1.34e-13	921	1104	16	202	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.
pfam13231	PMT_2	4.96e-11	951	1104	2	135	Dolichyl-phosphate-mannose-protein mannosyltransferase. This family contains members that are not captured by pfam02366.
COG4346	COG4346	5.35e-06	947	1328	132	416	Predicted membrane-bound dolichyl-phosphate-mannose-protein mannosyltransferase [Posttranslational modification, protein turnover, chaperones].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUC68008.1	3.52e-301	29	1361	29	1412
QTE68569.1	8.43e-297	30	1361	38	1392
QTE71236.1	5.28e-296	29	1361	14	1397
QTE75201.1	8.24e-296	29	1361	29	1412
QUA53848.1	1.48e-284	28	1361	32	1362

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
L8F4Z2	4.18e-19	917	1367	56	513	Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Mycolicibacterium smegmatis (strain MKD8) OX=1214915 GN=pmt PE=3 SV=1
P9WN04	1.34e-18	928	1367	73	519	Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=pmt PE=3 SV=2
P9WN05	1.34e-18	928	1367	73	519	Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=pmt PE=1 SV=2
Q8NRZ6	2.13e-09	951	1367	99	517	Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) OX=196627 GN=pmt PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000376	0.998698	0.000303	0.000201	0.000197	0.000182

TMHMM  Annotations     

start	end
216	233
246	268
303	322
329	348
358	380
387	405
420	437
450	472
487	509
514	536
566	588
601	618
623	638
645	667
682	704
743	765
971	993
1021	1040
1081	1103
1147	1169
1239	1261
1274	1293
1298	1320
1333	1355