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CAZyme Information
You are here: Home > Sequence: WP_001295307.1
Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | Signalp annotations | TMHMM annotations | PSSpred annotations | LipoP annotations

Basic Information help

Species Escherichia coli BW2952
CAZyme ID WP_001295307.1
CAZy Family PL22; 
CAZyme Description protein TolB
CAZyme Property
Protein Length Molecular Weight Isoelectric Point
430 45955.54 7.8281
Genome Property
Genome Assembly ID Genome Type GenBank ID RefSeq ID Length Total # of Proteins
GCF_000022345.1 Chromosome CP001396.1 NC_012759.1 4578159 4302
Gene Location Start: 679723;  End: 681015  Strand: +
View CDS CDS sequence
UniProt ID A7ZJC2;   B7ULM0;   B7LAE7;   P0A857;   B5YRE4;   B7NMV9;   B7MPN1;   C4ZWL5;   B1X6S1;   Q0TJV4;   P0A856;   B1IXY9;   P0A855;   B7N9Y9;   Q1REI5;   Q3Z464;   A0A024KR74;   A0A1S6T8J2;   A0A0H9UZS2;   C3TIH2;  
InterPro Annotation A7ZJC2;   B7ULM0;   B7LAE7;   P0A857;   B5YRE4;   B7NMV9;   B7MPN1;   C4ZWL5;   B1X6S1;   Q0TJV4;   P0A856;   B1IXY9;   P0A855;   B7N9Y9;   Q1REI5;   Q3Z464;   A0A024KR74;   A0A1S6T8J2;   A0A0H9UZS2;   C3TIH2;  

Genomic Context   If it does not show, please wait or refresh. help

Also see NCBI Genome Browser

Full Sequence      Download help

Enzyme Prediction      help

No EC number prediction in WP_001295307.1.

CAZyme Signature Domains help

Family Start End Evalue
PL22 290 401 4.3e-10

CDD Domains      download full data without filtering help

Cdd ID Domain E-Value Start End Domain Description
PRK03629 tolB 0.0 2 430
translocation protein TolB; Provisional
PRK01742 tolB 0.0 11 430
translocation protein TolB; Provisional
PRK04792 tolB 0.0 1 430
translocation protein TolB; Provisional
TIGR02800 propeller_TolB 0.0 13 427
tol-pal system beta propeller repeat protein TolB. Members of this protein family are the TolB periplasmic protein of Gram-negative bacteria. TolB is part of the Tol-Pal (peptidoglycan-associated lipoprotein) multiprotein complex, comprising five envelope proteins, TolQ, TolR, TolA, TolB and Pal, which form two complexes. The TolQ, TolR and TolA inner-membrane proteins interact via their transmembrane domains. The {beta}-propeller domain of the periplasmic protein TolB is responsible for its interaction with Pal. TolB also interacts with the outer-membrane peptidoglycan-associated proteins Lpp and OmpA. TolA undergoes a conformational change in response to changes in the proton-motive force, and interacts with Pal in an energy-dependent manner. The C-terminal periplasmic domain of TolA also interacts with the N-terminal domain of TolB. The Tol-PAL system is required for bacterial outer membrane integrity. E. coli TolB is involved in the tonB-independent uptake of group A colicins (colicins A, E1, E2, E3 and K), and is necessary for the colicins to reach their respective targets after initial binding to the bacteria. It is also involved in uptake of filamentous DNA. Study of its structure suggest that the TolB protein might be involved in the recycling of peptidoglycan or in its covalent linking with lipoproteins. The Tol-Pal system is also implicated in pathogenesis of E. coli, Haemophilus ducreyi , Salmonella enterica and Vibrio cholerae, but the mechanism(s) is unclear. [Transport and binding proteins, Other, Cellular processes, Pathogenesis]
PRK04922 tolB 1.36e-178 5 429
translocation protein TolB; Provisional

CAZyme Hits      help

WP_001295307.1 has no CAZyme hit.

PDB Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
3IAX_A 1.3e-160 1 430 1 430
Chain A, The Crystal Structure Of The Tolb Box Of Colicin A In Complex With Tolb Reveals Important Differences In The Recruitment Of The Common Tolb Translocation Portal Used By Group A Colicins
1C5K_A 1.3e-160 1 430 2 431
Chain A, The Structure Of Tolb, An Essential Component Of The Tol- Dependent Translocation System And Its Interactions With The Translocation Domain Of Colicin E9
2W8B_B 8.8e-149 22 430 1 409
Chain B, Crystal Structure Of Processed Tolb In Complex With Pal
2W8B_A 1.5e-148 22 430 1 409
Chain A, Crystal Structure Of Processed Tolb In Complex With Pal
2HQS_A 4.4e-148 23 430 2 409
Chain A, Crystal Structure Of TolbPAL COMPLEX

Swiss-Prot Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
sp|B7LAE7|TOLB_ECO55 1.1e-159 1 430 1 430
Protein TolB OS=Escherichia coli (strain 55989 / EAEC) GN=tolB PE=3 SV=1
sp|P0A855|TOLB_ECOLI 1.1e-159 1 430 1 430
Protein TolB OS=Escherichia coli (strain K12) GN=tolB PE=1 SV=1
sp|P0A856|TOLB_ECOL6 1.1e-159 1 430 1 430
Protein TolB OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) GN=tolB PE=3 SV=1
sp|B1IXY9|TOLB_ECOLC 1.1e-159 1 430 1 430
Protein TolB OS=Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) GN=tolB PE=3 SV=1
sp|B1X6S1|TOLB_ECODH 1.1e-159 1 430 1 430
Protein TolB OS=Escherichia coli (strain K12 / DH10B) GN=tolB PE=3 SV=1

SignalP Annotations help

Gram-positive-SP Gram-negative-SP
No signal petitide. No signal petitide.

TMHMM  Annotations      help

There is no transmembrane helices in WP_001295307.1.

PSSpred  Annotations      download dat.ss data help

color explanation:  coil(C), helix(H), beta(E)

LipoP Annotations      download full data without filtering help

class score margin cleavage
SpI 7.66433 7.865243 21-22