Basic Information | |
---|---|
Species | Ricinus communis |
Cazyme ID | 35285.m000016 |
Family | GH43 |
Protein Properties | Length: 316 Molecular Weight: 36538.1 Isoelectric Point: 5.2107 |
Chromosome | Chromosome/Scaffold: 35285 Start: 71 End: 1021 |
Description | |
View CDS |
External Links |
---|
NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
---|---|---|---|
Family | Start | End | Evalue |
GH43 | 8 | 308 | 8.50588e-43 |
PLIEQRADPYIYKHTDGYYYFTASVPQYDRIELRRAKTIAELATAPTVDAWIKPDTGPYSELIWAPELHFNMGAWYVYFAAAPSREIKYKLFQHRMYAIR NTNANPLEGTWEFMGQVDTGIDTFCLDATTFNHNGVLYYLWAQKDNEIEGNSNLYIAKMKTPWQIEGKPVLLSKPEHDWEIRGFWVNEGPSVLRRNGKIF ISYSASATDENYAMGLLWADENADLLDPAVWTKSREPVFRTCYDHGIYGPGHNSFTYAEDGDTVMLVYHARTYTEIVGDPLWNPDRHTFVKPLRWDENGM P |
Full Sequence |
---|
Protein Sequence Length: 316 Download |
MQDTVLKPLI EQRADPYIYK HTDGYYYFTA SVPQYDRIEL RRAKTIAELA TAPTVDAWIK 60 PDTGPYSELI WAPELHFNMG AWYVYFAAAP SREIKYKLFQ HRMYAIRNTN ANPLEGTWEF 120 MGQVDTGIDT FCLDATTFNH NGVLYYLWAQ KDNEIEGNSN LYIAKMKTPW QIEGKPVLLS 180 KPEHDWEIRG FWVNEGPSVL RRNGKIFISY SASATDENYA MGLLWADENA DLLDPAVWTK 240 SREPVFRTCY DHGIYGPGHN SFTYAEDGDT VMLVYHARTY TEIVGDPLWN PDRHTFVKPL 300 RWDENGMPVF GRPSTL |
Functional Domains Download unfiltered results here | ||||||||
---|---|---|---|---|---|---|---|---|
Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd08978 | GH_F | 2.0e-23 | 14 | 302 | 293 | + Glycosyl hydrolase families 43 and 62 form CAZY clan GH-F. This glycosyl hydrolase clan F (according to carbohydrate-active enzymes database (CAZY)) includes family 43 (GH43) and 62 (GH62). GH43 includes enzymes with beta-xylosidase (EC 3.2.1.37), beta-1,3-xylosidase (EC 3.2.1.-), alpha-L-arabinofuranosidase (EC 3.2.1.55), arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), endo-alpha-L-arabinanases (beta-xylanases) and galactan 1,3-beta-galactosidase (EC 3.2.1.145) activities. GH62 includes enzymes characterized as arabinofuranosidases (alpha-L-arabinofuranosidases; EC 3.2.1.55) that specifically cleave either alpha-1,2 or alpha-1,3-L-arabinofuranose side chains from xylans. GH43 are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many of the enzymes in this family display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. GH62 are also predicted to be inverting enzymes. A common structural feature of both, GH43 and GH62 enzymes, is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
cd08991 | GH43_bXyl_2 | 1.0e-24 | 14 | 311 | 319 | + Glycosyl hydrolase family 43. This glycosyl hydrolase family 43 (GH43) includes enzymes that have been characterized with xylan-digesting beta-xylosidase (EC 3.2.1.37) and xylanase (endo-alpha-L-arabinanase) activities. These are all inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many of the enzymes in this family display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam04616 | Glyco_hydro_43 | 2.0e-64 | 8 | 308 | 304 | + Glycosyl hydrolases family 43. The glycosyl hydrolase family 43 contains members that are arabinanase. Rabinanases hydrolyses the alpha-1,5-linked L-arabinofuranoside backbone of plant cell wall arabinans. The structure of arabinanase Arb43A from Cellvibrio japonicus reveals a five-bladed beta-propeller fold. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
cd08980 | GH43_1 | 1.0e-142 | 14 | 311 | 299 | + Glycosyl hydrolase family 43. This glycosyl hydrolase family 43 (GH43) includes enzymes with beta-xylosidase (EC 3.2.1.37) and alpha-L-arabinofuranosidase (EC 3.2.1.55) and possibly bifunctional xylosidase/arabinofuranosidase activities. These are inverting enzymes (i.e. they invert the stereochemistry of the anomeric carbon atom of the substrate) that have an aspartate as the catalytic general base, a glutamate as the catalytic general acid and another aspartate that is responsible for pKa modulation and orienting the catalytic acid. Many of the enzymes in this family display both alpha-L-arabinofuranosidase and beta-D-xylosidase activity using aryl-glycosides as substrates. A common structural feature of GH43 enzymes is a 5-bladed beta-propeller domain that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
COG3940 | COG3940 | 2.0e-165 | 1 | 313 | 313 | + Predicted beta-xylosidase [General function prediction only] |
Gene Ontology | |
---|---|
GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
Annotations - PDB Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3k1u_A | 0 | 2 | 315 | 6 | 319 | A Chain A, Beta-Xylosidase, Family 43 Glycosyl Hydrolase From Clostridium Acetobutylicum |
PDB | 3aki_A | 0 | 4 | 313 | 10 | 315 | A Chain A, Beta-Xylosidase, Family 43 Glycosyl Hydrolase From Clostridium Acetobutylicum |
PDB | 3akh_A | 0 | 4 | 313 | 10 | 315 | A Chain A, Beta-Xylosidase, Family 43 Glycosyl Hydrolase From Clostridium Acetobutylicum |
PDB | 3akg_A | 0 | 4 | 313 | 10 | 315 | A Chain A, Beta-Xylosidase, Family 43 Glycosyl Hydrolase From Clostridium Acetobutylicum |
PDB | 3akf_A | 0 | 4 | 313 | 10 | 315 | A Chain A, Crystal Structure Of Exo-1,5-Alpha-L-Arabinofuranosidase |
Orthologous Group | |||||
---|---|---|---|---|---|
Species | ID | ||||
Ricinus communis | 32250.m000015 | 32604.m000021 |
Sequence Alignments (This image is cropped. Click for full image.) |
---|