Basic Information | |
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Species | Citrus clementina |
Cazyme ID | Ciclev10017787m |
Family | GT20 |
Protein Properties | Length: 869 Molecular Weight: 98359.5 Isoelectric Point: 6.9534 |
Chromosome | Chromosome/Scaffold: 2 Start: 34649712 End: 34658144 |
Description | trehalose-6-phosphate synthase |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GT20 | 78 | 348 | 0 |
RCKQRLLVVANRLPVSAVRRGEDSWQLEISVGGLVRVREFEARWIGWAGVNVPDEIGQKALTKALAEKRCIPVFLDEETVHQYYNGYCNNMLWPLFHYLG LPQEDRLATTRSFQSQFDAYKRANQMFAKVVNNIYEEGDVVWCHDYHLMFLPQCLKEYNNNMKVGWFLHTPFPSSEIHRTLPSRTELLRSVLAADLVGFH TYDYARHFVSACTRILGLEGTPEGVEDQGRLTRVAAFPIGIDSDRFVRALELPQVQDHINELKERFAGRKV | |||
GT20 | 351 | 497 | 0 |
VYQKLTSQVHEIVGRINGRYGTLTTVPIHHLDRSLDFHALCALYAITDVALVTSLRDGMNLVSYEFVACQASKKGVLILSEFAGAAQSLGAGAILVNPWN ITEVASSIGYALNMPADEREKRHLHNFMHVTTHTSQEWAATFVSELN |
Full Sequence |
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Protein Sequence Length: 869 Download |
MPGNKYNSSP LTPRTRLERL LRERELRKNR SFLQNEGEPD FFASESFLNA SESWGQIDEE 60 EFAGTRSSTD NGERLEGRCK QRLLVVANRL PVSAVRRGED SWQLEISVGG LVRVREFEAR 120 WIGWAGVNVP DEIGQKALTK ALAEKRCIPV FLDEETVHQY YNGYCNNMLW PLFHYLGLPQ 180 EDRLATTRSF QSQFDAYKRA NQMFAKVVNN IYEEGDVVWC HDYHLMFLPQ CLKEYNNNMK 240 VGWFLHTPFP SSEIHRTLPS RTELLRSVLA ADLVGFHTYD YARHFVSACT RILGLEGTPE 300 GVEDQGRLTR VAAFPIGIDS DRFVRALELP QVQDHINELK ERFAGRKVGS VYQKLTSQVH 360 EIVGRINGRY GTLTTVPIHH LDRSLDFHAL CALYAITDVA LVTSLRDGMN LVSYEFVACQ 420 ASKKGVLILS EFAGAAQSLG AGAILVNPWN ITEVASSIGY ALNMPADERE KRHLHNFMHV 480 TTHTSQEWAA TFVSELNDTI VEAQIRTRQV PPSLPIKGAV DSYLQSNNRL LILGFNATLT 540 APVDFLGRRG GQIREMEPKL HPDLKEPLKR LCDDPMTTVV VLSGSDRNVL DDNFGEYNMW 600 LAAENGMFLR LTTGEWMTTM PENLNMDWVD SVKHVFEYFT ERTPRSHFEV RETSLVWNYK 660 YADLEFGRLQ ARDILQHLWS GPISNASVDV VQGGRSVEVR AVGVTKGAAI DRILGEIVRH 720 KGLKTPIDYV LCIGHFLQKD EDIYTFFEPE LPFESPAGPR ANAADHLRRS SISQIPQAKS 780 GPKVRFRKQR SLSSIERKVS NYLTGGIWRP AIRDRMTLNE GSSVLDLRGE NYFSCTVSRK 840 RSNARYFLGS SGDVVTLLNE LAECPQSN* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PLN03064 | PLN03064 | 0 | 1 | 863 | 932 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
PLN03063 | PLN03063 | 0 | 80 | 862 | 833 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
PRK14501 | PRK14501 | 0 | 82 | 734 | 712 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional | ||
TIGR02400 | trehalose_OtsA | 0 | 82 | 498 | 469 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
pfam00982 | Glyco_transf_20 | 0 | 81 | 498 | 474 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0005992 | trehalose biosynthetic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CAA69879.1 | 0 | 1 | 867 | 1 | 941 | trehalose-6-phosphate synthase [Arabidopsis thaliana] |
RefSeq | NP_177979.1 | 0 | 1 | 867 | 1 | 941 | ATTPS1 (TREHALOSE-6-PHOSPHATE SYNTHASE); alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
RefSeq | XP_002305707.1 | 0 | 21 | 868 | 1 | 899 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002331300.1 | 0 | 1 | 862 | 1 | 918 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002522296.1 | 0 | 1 | 863 | 1 | 910 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2wtx_D | 0 | 82 | 508 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_C | 0 | 82 | 508 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_B | 0 | 82 | 508 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_A | 0 | 82 | 508 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 1uqu_B | 0 | 82 | 508 | 3 | 466 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
ES813375 | 360 | 393 | 752 | 0 |
CX546763 | 292 | 64 | 348 | 0 |
CV711307 | 273 | 80 | 348 | 0 |
DV705833 | 283 | 465 | 747 | 0 |
CX545499 | 288 | 352 | 639 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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