Basic Information | |
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Species | Citrus clementina |
Cazyme ID | Ciclev10030780m |
Family | CBM45 |
Protein Properties | Length: 755 Molecular Weight: 85395 Isoelectric Point: 5.2571 |
Chromosome | Chromosome/Scaffold: 4 Start: 23575399 End: 23582495 |
Description | alpha-amylase-like 3 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
CBM45 | 171 | 247 | 1.1e-23 |
VHWGVCRDDSKNWEIPAEPYPPETIVFKNKALRTLLQPKEGGKGCSRLFTVDEEFAGFLFVLKLNENTWLKCMENDF | |||
GH13 | 385 | 674 | 1.1e-36 |
EKATELSSLGFSVIWLPPPTESVSPEGYMPRDLYNLSSRYGNIDELKDVVNKFHDVGMKILGDVVLNHRCAHYQNQNGVWNIFGGRLNWDDRAVVADDPH FQGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWLRNEIGYDGWRLDFVRGFWGGYVKDYLEATEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIIDW INAASGTAGAFDVTTKGILHSALDRCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGREMQGYAYILTHPGTPSVFY |
Full Sequence |
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Protein Sequence Length: 755 Download |
MRPPGSVSIK DYAIETPLKK LAEGDVFDQV KIDFDTRSDI AAINFVLKDE ETGAWYQHRG 60 RDFKVPLVDY LQHDGNVIGT KSTFGLWPGA LGQLSKMILK ADTSQSGIQD SSSESCELKQ 120 ENKHLEGFYE ELPIVKEIII ENTVSVSVRK CPETAKTLLN LETDLTGDVV VHWGVCRDDS 180 KNWEIPAEPY PPETIVFKNK ALRTLLQPKE GGKGCSRLFT VDEEFAGFLF VLKLNENTWL 240 KCMENDFYIP LTSSSCLPAE SVQEMLIPGK AEEATQEVSQ TAYTAGIIKE IRNLVSDFSS 300 DISRKTKSKE AQKSILLEIE KLAAEAYSIF RTSAPTFFEE AAVELEESKP PAKISPGTGT 360 GFEILCQGFN WESHKSGRWY TELKEKATEL SSLGFSVIWL PPPTESVSPE GYMPRDLYNL 420 SSRYGNIDEL KDVVNKFHDV GMKILGDVVL NHRCAHYQNQ NGVWNIFGGR LNWDDRAVVA 480 DDPHFQGRGN KSSGDNFHAA PNIDHSQDFV RKDIKEWLCW LRNEIGYDGW RLDFVRGFWG 540 GYVKDYLEAT EPYFAVGEYW DSLSYTYGEM DHNQDAHRQR IIDWINAASG TAGAFDVTTK 600 GILHSALDRC EYWRLSDEKG KPPGVVGWWP SRAVTFIENH DTGSTQGHWR FPGGREMQGY 660 AYILTHPGTP SVFYDHIFSH YRQEIEALLS VRKRNKIHCR SRVEIVKAER DVYAAIIDEK 720 VAMKLGPGHY EPPSGSQNWC FVTEGRDYKV WEAA* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PLN02784 | PLN02784 | 5.0e-5 | 126 | 256 | 138 | + alpha-amylase | ||
PLN00196 | PLN00196 | 4.0e-137 | 363 | 754 | 409 | + alpha-amylase; Provisional | ||
cd11314 | AmyAc_arch_bac_plant_AmyA | 3.0e-166 | 364 | 703 | 343 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN02361 | PLN02361 | 4.0e-170 | 361 | 752 | 398 | + alpha-amylase | ||
PLN02784 | PLN02784 | 0 | 1 | 754 | 755 | + alpha-amylase |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004556 | alpha-amylase activity |
GO:0005509 | calcium ion binding |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAX33231.1 | 0 | 1 | 754 | 144 | 901 | plastid alpha-amylase [Malus x domestica] |
EMBL | CBI32016.1 | 0 | 1 | 754 | 138 | 885 | unnamed protein product [Vitis vinifera] |
RefSeq | XP_002520134.1 | 0 | 1 | 754 | 145 | 900 | alpha-amylase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2qpu_C | 0 | 363 | 752 | 2 | 403 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
PDB | 2qpu_B | 0 | 363 | 752 | 2 | 403 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
PDB | 2qpu_A | 0 | 363 | 752 | 2 | 403 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
PDB | 3bsg_A | 0 | 363 | 752 | 2 | 403 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
PDB | 1rpk_A | 0 | 363 | 752 | 2 | 403 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
EG631183 | 759 | 1 | 755 | 0 |
HO826981 | 407 | 349 | 755 | 0 |
DR932783 | 288 | 363 | 650 | 0 |
HO826981 | 30 | 318 | 347 | 0.44 |
EG631183 | 137 | 126 | 251 | 1.7 |
Sequence Alignments (This image is cropped. Click for full image.) |
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