| Basic Information | |
|---|---|
| Species | Chlamydomonas reinhardtii |
| Cazyme ID | Cre03.g189050.t1.3 |
| Family | GH99 |
| Protein Properties | Length: 463 Molecular Weight: 51625.1 Isoelectric Point: 5.4384 |
| Chromosome | Chromosome/Scaffold: 3 Start: 5808756 End: 5813837 |
| Description | |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH99 | 45 | 387 | 0 |
| VHAFYYLWYNEPSTGRWAHWDHAVLPHWDPATAAKYPQGIRFEPPDCLHSPYYPLRGPYSSTSQELIRQHLEEMAKYGIGILVASWWGPGWRQGSHDTQL INTDDRIEQVIREIEATSSPVRVAFHLEPYEGRTKQTVHEDLTYLSHKYKDSSAVARVNGRILYYVYDSYRLPASDWAELLVRNTGPINVRGTAADGFFL GLWLEKDDGDKNIGPGGFDGFYTYFSSEATSYASNPTNWPDLQRWAREHHRIFVPSLGPGYHDGKIRPWNMAARREREGIDRYKRLWDTAIALGVHFVSI TSYNEWGEGTQVEPAVPRPMRKDCAYLDYEPHSPFEFLRLTQE | |||
| Full Sequence |
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| Protein Sequence Length: 463 Download |
| MHVACTLERL KHRRRKCTTP LLSLALVVLA STCGRAAHIP ARYRVHAFYY LWYNEPSTGR 60 WAHWDHAVLP HWDPATAAKY PQGIRFEPPD CLHSPYYPLR GPYSSTSQEL IRQHLEEMAK 120 YGIGILVASW WGPGWRQGSH DTQLINTDDR IEQVIREIEA TSSPVRVAFH LEPYEGRTKQ 180 TVHEDLTYLS HKYKDSSAVA RVNGRILYYV YDSYRLPASD WAELLVRNTG PINVRGTAAD 240 GFFLGLWLEK DDGDKNIGPG GFDGFYTYFS SEATSYASNP TNWPDLQRWA REHHRIFVPS 300 LGPGYHDGKI RPWNMAARRE REGIDRYKRL WDTAIALGVH FVSITSYNEW GEGTQVEPAV 360 PRPMRKDCAY LDYEPHSPFE FLRLTQEGGE RLTAAAEQAA ADADALRGGD KEGAAEEVEE 420 EDEYADGDDD EDGAGSGSCG GEGAGGGEGG EEGGGNCGAS GH* 480 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd11577 | GH71 | 0.0007 | 192 | 379 | 200 | + Glycoside hydrolase family 71. This family of glycoside hydrolases 71 (following the CAZY nomenclature) function as alpha-1,3-glucanases (mutanases, EC 3.2.1.59). They appear to have an endo-hydrolytic mode of enzymatic activity and bacterial members are investigated as candidates for the development of dental caries treatments.The member from fission yeast, endo-alpha-1,3-glucanase Agn1p, plays a vital role in daughter cell separation, while Agn2p has been associated with endolysis of the ascus wall. | ||
| cd11578 | GH99_GH71_like_1 | 9.0e-14 | 91 | 363 | 308 | + Uncharacterized glycoside hydrolase family 99-like domain. This family of putative glycoside hydrolases resembles glycosyl hydrolase families 71 and 99 (following the CAZY nomenclature) and may share a similar catalytic site and mechanism. | ||
| cd11575 | GH99_GH71_like_3 | 5.0e-14 | 45 | 360 | 337 | + Uncharacterized glycoside hydrolase family 99-like domain. This family of putative glycoside hydrolases resembles glycosyl hydrolase families 71 and 99 (following the CAZY nomenclature) and may share a similar catalytic site and mechanism. | ||
| cd11573 | GH99_GH71_like | 1.0e-35 | 109 | 385 | 285 | + Glycoside hydrolase families 71, 99, and related domains. This superfamily of glycoside hydrolases contains families GH71 and GH99 (following the CAZY nomenclature), as well as other members with undefined function and specificity. | ||
| cd11574 | GH99 | 3.0e-126 | 45 | 387 | 347 | + Glycoside hydrolase family 99, an endo-alpha-1,2-mannosidase. This family of glycoside hydrolases 99 (following the CAZY nomenclature) includes endo-alpha-1,2-mannosidase (EC 3.2.1.130), which is an important membrane-associated eukaryotic enzyme involved in the maturation of N-linked glycans. Specifically, it cleaves mannoside linkages internal to N-linked glycan chains by hydrolyzing an alpha-1,2-mannosidic bond between a glucose-substituted mannose and the remainder of the chain. The biological function and significance of the soluble bacterial orthologs, which may have obtained the genes via horizontal transfer, is not clear. | ||
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| RefSeq | NP_001038579.1 | 0 | 43 | 398 | 88 | 439 | glycoprotein endo-alpha-1,2-mannosidase-like protein [Danio rerio] |
| RefSeq | XP_001056614.1 | 0 | 45 | 398 | 100 | 449 | PREDICTED: similar to mannosidase, endo-alpha [Rattus norvegicus] |
| RefSeq | XP_001170001.1 | 0 | 45 | 398 | 105 | 454 | PREDICTED: similar to mannosidase, endo-alpha-like isoform 3 [Pan troglodytes] |
| RefSeq | XP_001364714.1 | 0 | 40 | 398 | 98 | 453 | PREDICTED: similar to mannosidase, endo-alpha-like [Monodelphis domestica] |
| RefSeq | XP_001691860.1 | 0 | 53 | 423 | 32 | 402 | hypothetical protein CHLREDRAFT_145451 [Chlamydomonas reinhardtii] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 4acz_B | 0 | 36 | 375 | 33 | 354 | A Chain A, Structure Of The Gh99 Endo-Alpha-Mannosidase From Bacteroides Thetaiotaomicron |
| PDB | 4ad0_D | 0 | 36 | 375 | 33 | 354 | A Chain A, Structure Of The Gh99 Endo-Alpha-Mannosidase From Bacteroides Thetaiotaomicron |
| PDB | 4ad0_C | 0 | 36 | 375 | 33 | 354 | A Chain A, Structure Of The Gh99 Endo-Alpha-Mannosidase From Bacteroides Thetaiotaomicron |
| PDB | 4ad0_B | 0 | 36 | 375 | 33 | 354 | A Chain A, Structure Of The Gh99 Endo-Alpha-Mannosidase From Bacteroides Thetaiotaomicron |
| PDB | 4ad0_A | 0 | 36 | 375 | 33 | 354 | A Chain A, Structure Of The Gh99 Endo-Alpha-Mannosidase From Bacteroides Thetaiotaomicron |
| Transmembrane Domains | |||||
|---|---|---|---|---|---|
| Start | End | ||||
| 20 | 39 | ||||
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| HO627855 | 354 | 42 | 387 | 0 |
| GW595129 | 200 | 188 | 387 | 0 |
| HO434704 | 184 | 204 | 387 | 0 |
| HO639020 | 147 | 42 | 186 | 1e-39 |
| EH669148 | 234 | 45 | 267 | 2e-38 |
| Orthologous Group | |||||
|---|---|---|---|---|---|
| Species | ID | ||||
| Volvox carteri | Vocar20003404m | ||||
| Sequence Alignments (This image is cropped. Click for full image.) |
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| Phylogeny |
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