| Basic Information | |
|---|---|
| Species | Malus domestica |
| Cazyme ID | MDP0000170967 |
| Family | GT13 |
| Protein Properties | Length: 359 Molecular Weight: 41107.6 Isoelectric Point: 8.8523 |
| Chromosome | Chromosome/Scaffold: 002957516 Start: 4438 End: 9640 |
| Description | alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase, putative |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT13 | 22 | 209 | 0 |
| IQMRLFATQSQYADRLADAIESENHCTSQLRTFIDRISLQQGQIVSLDEEKQRLEQECGQLKVLVQELERKGLGRLIDKNQVPVAAVVIMACNRADYLER TVKSVLKYQSSVASKFPLFVSQDGSHPAVKQXALSYDQLTYMQHLDYXPVHAERPGELIAYYKIARHYKWALDGLFYKHKFSRVIILE | |||
| GT13 | 230 | 351 | 0 |
| LGSSLGQFFKQYLEPIKLNDVPVDWKSMDLSYLMEDKYLKHFADIVKKAKPIHGSDLVLKARNIGGDVRIQYNDQPDFEYIAGQFGIFEEWKDGVPRTAY KGIVVFRYHIPQRIFLVGPDSL | |||
| Full Sequence |
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| Protein Sequence Length: 359 Download |
| MGHCHHCASS KNSRAAAAFI YIQMRLFATQ SQYADRLADA IESENHCTSQ LRTFIDRISL 60 QQGQIVSLDE EKQRLEQECG QLKVLVQELE RKGLGRLIDK NQVPVAAVVI MACNRADYLE 120 RTVKSVLKYQ SSVASKFPLF VSQDGSHPAV KQXALSYDQL TYMQHLDYXP VHAERPGELI 180 AYYKIARHYK WALDGLFYKH KFSRVIILEG LSWPFXRGMT MGKNSLCMIL GSSLGQFFKQ 240 YLEPIKLNDV PVDWKSMDLS YLMEDKYLKH FADIVKKAKP IHGSDLVLKA RNIGGDVRIQ 300 YNDQPDFEYI AGQFGIFEEW KDGVPRTAYK GIVVFRYHIP QRIFLVGPDS LKQLGVNDS 360 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd00761 | Glyco_tranf_GTA_type | 0.006 | 108 | 157 | 50 | + Glycosyltransferase family A (GT-A) includes diverse families of glycosyl transferases with a common GT-A type structural fold. Glycosyltransferases (GTs) are enzymes that synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein. Based on the stereochemistry of the donor and acceptor molecules, GTs are classified as either retaining or inverting enzymes. To date, all GT structures adopt one of two possible folds, termed GT-A fold and GT-B fold. This hierarchy includes diverse families of glycosyl transferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. The majority of the proteins in this superfamily are Glycosyltransferase family 2 (GT-2) proteins. But it also includes families GT-43, GT-6, GT-8, GT13 and GT-7; which are evolutionarily related to GT-2 and share structure similarities. | ||
| cd02514 | GT13_GLCNAC-TI | 7.0e-41 | 217 | 350 | 137 | + GT13_GLCNAC-TI is involved in an essential step in the synthesis of complex or hybrid-type N-linked oligosaccharides. Alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (GLCNAC-T I , GNT-I) transfers N-acetyl-D-glucosamine from UDP to high-mannose glycoprotein N-oligosaccharide, an essential step in the synthesis of complex or hybrid-type N-linked oligosaccharides. The enzyme is an integral membrane protein localized to the Golgi apparatus. The catalytic domain is located at the C-terminus. These proteins are members of the glycosy transferase family 13. | ||
| cd02514 | GT13_GLCNAC-TI | 7.0e-42 | 105 | 209 | 106 | + GT13_GLCNAC-TI is involved in an essential step in the synthesis of complex or hybrid-type N-linked oligosaccharides. Alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (GLCNAC-T I , GNT-I) transfers N-acetyl-D-glucosamine from UDP to high-mannose glycoprotein N-oligosaccharide, an essential step in the synthesis of complex or hybrid-type N-linked oligosaccharides. The enzyme is an integral membrane protein localized to the Golgi apparatus. The catalytic domain is located at the C-terminus. These proteins are members of the glycosy transferase family 13. | ||
| pfam03071 | GNT-I | 1.0e-59 | 217 | 358 | 142 | + GNT-I family. Alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (GNT-I, GLCNAC-T I) EC:2.4.1.101 transfers N-acetyl-D-glucosamine from UDP to high-mannose glycoprotein N-oligosaccharide. This is an essential step in the synthesis of complex or hybrid-type N-linked oligosaccharides. The enzyme is an integral membrane protein localised to the Golgi apparatus, and is probably distributed in all tissues. The catalytic domain is located at the C-terminus. | ||
| pfam03071 | GNT-I | 5.0e-81 | 15 | 209 | 199 | + GNT-I family. Alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (GNT-I, GLCNAC-T I) EC:2.4.1.101 transfers N-acetyl-D-glucosamine from UDP to high-mannose glycoprotein N-oligosaccharide. This is an essential step in the synthesis of complex or hybrid-type N-linked oligosaccharides. The enzyme is an integral membrane protein localised to the Golgi apparatus, and is probably distributed in all tissues. The catalytic domain is located at the C-terminus. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0000139 | Golgi membrane |
| GO:0003827 | alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity |
| GO:0006487 | protein N-linked glycosylation |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACU18645.1 | 0 | 15 | 209 | 15 | 209 | unknown [Glycine max] |
| GenBank | ACU18645.1 | 2e-21 | 231 | 289 | 316 | 374 | unknown [Glycine max] |
| GenBank | EEC74310.1 | 0 | 42 | 356 | 25 | 340 | hypothetical protein OsI_09579 [Oryza sativa Indica Group] |
| RefSeq | XP_002266012.1 | 0 | 15 | 209 | 15 | 209 | PREDICTED: hypothetical protein, partial [Vitis vinifera] |
| RefSeq | XP_002527145.1 | 0 | 15 | 321 | 15 | 346 | alpha-1,3-mannosyl-glycoprotein beta-1, 2-n-acetylglucosaminyltransferase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1foa_A | 0.000000000000003 | 217 | 350 | 213 | 339 | A Chain A, Crystal Structure Of N-acetylglucosaminyltransferase I |
| PDB | 1foa_A | 0.000000002 | 102 | 209 | 5 | 112 | A Chain A, Crystal Structure Of N-acetylglucosaminyltransferase I |
| PDB | 1fo9_A | 0.000000000000003 | 217 | 350 | 213 | 339 | A Chain A, Crystal Structure Of N-acetylglucosaminyltransferase I |
| PDB | 1fo9_A | 0.000000002 | 102 | 209 | 5 | 112 | A Chain A, Crystal Structure Of N-acetylglucosaminyltransferase I |
| PDB | 1fo8_A | 0.000000000000004 | 217 | 350 | 208 | 334 | A Chain A, Crystal Structure Of N-Acetylglucosaminyltransferase I |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EB117484 | 166 | 44 | 209 | 0 |
| FG911794 | 195 | 15 | 209 | 0 |
| FG989519 | 195 | 15 | 209 | 0 |
| FG161912 | 195 | 15 | 209 | 0 |
| EY722460 | 195 | 15 | 209 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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| Phylogeny |
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