Basic Information | |
---|---|
Species | Malus domestica |
Cazyme ID | MDP0000227381 |
Family | GT20 |
Protein Properties | Length: 891 Molecular Weight: 99958.8 Isoelectric Point: 7.1914 |
Chromosome | Chromosome/Scaffold: 0351947 Start: 1922 End: 7896 |
Description | trehalose-6-phosphate synthase |
View CDS |
External Links |
---|
NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
---|---|---|---|
Family | Start | End | Evalue |
GT20 | 89 | 322 | 0 |
KQRLLVVANRLPVSAVRKGEDSWQLEISVGGLVSALLGVKEFDARWIGWAGVNVPDSVGQKALTKALAEKRCIPVFLDEEIVHQYYNGYCNNILWPLFHY LGLPQEDRLATTRSFQSQFDAYKKANQMFADVVHEHYQEGDVVWCHDYHLMFLPKCLKEHNXKMKVGWFLHTPFPSSEIHRTLPSRSELLRSVLAADLVG HFVSACTRILGLEGTPEGVENQGKLTRVAAVMLG | |||
GT20 | 319 | 514 | 0 |
VMLGVDRLDMIKGIPQKILAFEKFLEENLNWRDKVVLLQIAVPTRTDVPEYQKLTSQVHEIVGRINGRFGTLTAVPIHHLDRSLDFHALCALYAVTDVAL VTSLRDGMNLVSYEFVACQASKKGVLILSEFAGAAQSLGAGAILVNPWNITEVAASIGYALNMAADEREKRHHHNFMHVTTHTSQEWAATFVSELN |
Full Sequence |
---|
Protein Sequence Length: 891 Download |
MPGNNYNCNP STPRSRLERL LRERELRRSN RVSQSNXDGN RQAEFVGSDA FFTEGENLGV 60 SSEDDFPEGV SATKIFIDGC EWKEGRPPKQ RLLVVANRLP VSAVRKGEDS WQLEISVGGL 120 VSALLGVKEF DARWIGWAGV NVPDSVGQKA LTKALAEKRC IPVFLDEEIV HQYYNGYCNN 180 ILWPLFHYLG LPQEDRLATT RSFQSQFDAY KKANQMFADV VHEHYQEGDV VWCHDYHLMF 240 LPKCLKEHNX KMKVGWFLHT PFPSSEIHRT LPSRSELLRS VLAADLVGHF VSACTRILGL 300 EGTPEGVENQ GKLTRVAAVM LGVDRLDMIK GIPQKILAFE KFLEENLNWR DKVVLLQIAV 360 PTRTDVPEYQ KLTSQVHEIV GRINGRFGTL TAVPIHHLDR SLDFHALCAL YAVTDVALVT 420 SLRDGMNLVS YEFVACQASK KGVLILSEFA GAAQSLGAGA ILVNPWNITE VAASIGYALN 480 MAADEREKRH HHNFMHVTTH TSQEWAATFV SELNDTIVEA QLRTRQVPPI LPIKVAVDRY 540 LQSSNRLLIL GFNATLTEPK DTFGRRGGQI REMELKLHPD LKEPLKKICD DSKTTIVVLS 600 GSDRSVLDYL PIQNFGDYNM WLAAENGMFL RLTMGEWMTT MPENLNMDWV DSVKHVFEYF 660 TERTPRSHFE LRETSLVWNY KYADIEFGRL QARDLLQHLW TGPISNASVD VVQGGRSVEV 720 RAVGVTKGAA IDRILGEIVH NKGMKAPIDY VLCIGHFLPK DEDVYTFFEP ELPVEAPPTV 780 PTRPPSPTPG MSSAPLPRIS TSKSNSKAAR LKKQRSLSTL EKRASIHAIG SAWRPTLMRD 840 KMSLHEGSSV LDLKGDNYFS CAVGRKRSSA RYLLGSSDDV VTLLKELAEG S 900 |
Functional Domains Download unfiltered results here | ||||||||
---|---|---|---|---|---|---|---|---|
Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PLN03064 | PLN03064 | 0 | 1 | 891 | 938 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
PLN03063 | PLN03063 | 0 | 82 | 888 | 849 | + alpha,alpha-trehalose-phosphate synthase (UDP-forming); Provisional | ||
TIGR02400 | trehalose_OtsA | 0 | 91 | 515 | 469 | + alpha,alpha-trehalose-phosphate synthase [UDP-forming]. This enzyme catalyzes the key, penultimate step in biosynthesis of trehalose, a compatible solute made as an osmoprotectant in some species in all three domains of life. The gene symbol OtsA stands for osmotically regulated trehalose synthesis A. Trehalose helps protect against both osmotic and thermal stresses, and is made from two glucose subunits. This model excludes glucosylglycerol-phosphate synthase, an enzyme of an analogous osmoprotectant system in many cyanobacterial strains. This model does not identify archaeal examples, as they are more divergent than glucosylglycerol-phosphate synthase. Sequences that score in the gray zone between the trusted and noise cutoffs include a number of yeast multidomain proteins in which the N-terminal domain may be functionally equivalent to this family. The gray zone also includes the OtsA of Cornyebacterium glutamicum (and related species), shown to be responsible for synthesis of only trace amounts of trehalose while the majority is synthesized by the TreYZ pathway; the significance of OtsA in this species is unclear (see Wolf, et al., PMID:12890033) [Cellular processes, Adaptations to atypical conditions]. | ||
pfam00982 | Glyco_transf_20 | 0 | 90 | 515 | 474 | + Glycosyltransferase family 20. Members of this family belong to glycosyl transferase family 20. OtsA (Trehalose-6-phosphate synthase) is homologous to regions in the subunits of yeast trehalose-6-phosphate synthase/phosphate complex. | ||
PRK14501 | PRK14501 | 0 | 91 | 755 | 714 | + putative bifunctional trehalose-6-phosphate synthase/HAD hydrolase subfamily IIB; Provisional |
Gene Ontology | |
---|---|
GO Term | Description |
GO:0003824 | catalytic activity |
GO:0005992 | trehalose biosynthetic process |
Annotations - NR Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CAA69879.1 | 0 | 1 | 889 | 1 | 937 | trehalose-6-phosphate synthase [Arabidopsis thaliana] |
RefSeq | NP_177979.1 | 0 | 1 | 889 | 1 | 937 | ATTPS1 (TREHALOSE-6-PHOSPHATE SYNTHASE); alpha,alpha-trehalose-phosphate synthase (UDP-forming)/ transferase, transferring glycosyl groups [Arabidopsis thaliana] |
RefSeq | XP_002305707.1 | 0 | 21 | 890 | 1 | 895 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002331300.1 | 0 | 1 | 891 | 1 | 921 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002522296.1 | 0 | 1 | 891 | 1 | 912 | trehalose-6-phosphate synthase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
---|---|---|---|---|---|---|---|
Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2wtx_D | 0 | 91 | 525 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_C | 0 | 91 | 525 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_B | 0 | 91 | 525 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 2wtx_A | 0 | 91 | 525 | 3 | 466 | A Chain A, Insight Into The Mechanism Of Enzymatic Glycosyltransfer With Retention Through The Synthesis And Analysis Of Bisubstrate Glycomimetics Of Trehalose-6-Phosphate Synthase |
PDB | 1uqu_B | 0 | 91 | 525 | 3 | 466 | A Chain A, Trehalose-6-Phosphate From E. Coli Bound With Udp-Glucose. |
EST Download unfiltered results here | ||||
---|---|---|---|---|
Hit | Length | Start | End | EValue |
ES813375 | 364 | 410 | 773 | 0 |
CX545499 | 309 | 352 | 660 | 0 |
DV705833 | 286 | 483 | 768 | 0 |
GO265628 | 307 | 404 | 710 | 0 |
GE582665 | 287 | 385 | 670 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
---|