Basic Information | |
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Species | Malus domestica |
Cazyme ID | MDP0000280501 |
Family | PL4 |
Protein Properties | Length: 624 Molecular Weight: 71986.9 Isoelectric Point: 4.9114 |
Chromosome | Chromosome/Scaffold: 008084496 Start: 7722 End: 13291 |
Description | Rhamnogalacturonate lyase family protein |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
PL4 | 6 | 222 | 0 |
VRLIIQDRYVVMDNGILQVTLSNPDGIVTRIQYSGIDNLLEVLNEEVNRGYWDLVWSEAGSVGTTGTFDVIKGTKFKVRVENEEQVEISFTRKWNPSQEG KLVPLNIDKRFIMLRNSSGFYSYAIYEHLKEWPPFNLPQTRITFKLRKEKFHYMAIADNRQRYMPLPDDRSPERSQALAYPEAVLLVDPIEPEFKGEVDD KYEYSCENQNLHVHGWM | |||
PL4 | 221 | 597 | 0 |
WMFLSAHYSGEDLVLKXKPDEPWKKIFGPVFIYLNSXSDATDPSPLWDDAKKQMMTEVQSWPYKFPASEDYPPSDQRGNVKGRIQILDSFCEIRPKFYLN LXMSRYVSKDXVPGXGAYVGLAPPGDVGSWQRDCKGYQFWTRADEKGYYSIQNIREGDYNLYAWCPGFIGDYRYDATISITAGCDIDVDDIVYEPPRDGP TLWEIGIPDRSAAEFYVPDPNPMYINKLYVNHPDRFRQYGLWERYQELYPDEDLIYSIGTSDYTKDWFFAQVPRKKDEYTYQGTTWQIKFQLKHFSESDT LKLRIALATANIAELQIRINDSKADPPLFTTGVIGNDNTIARHGIHGLYRLYNIDIPGTLLVEGANTLFLTQPMSSS |
Full Sequence |
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Protein Sequence Length: 624 Download |
MSSQRVRLII QDRYVVMDNG ILQVTLSNPD GIVTRIQYSG IDNLLEVLNE EVNRGYWDLV 60 WSEAGSVGTT GTFDVIKGTK FKVRVENEEQ VEISFTRKWN PSQEGKLVPL NIDKRFIMLR 120 NSSGFYSYAI YEHLKEWPPF NLPQTRITFK LRKEKFHYMA IADNRQRYMP LPDDRSPERS 180 QALAYPEAVL LVDPIEPEFK GEVDDKYEYS CENQNLHVHG WMFLSAHYSG EDLVLKXKPD 240 EPWKKIFGPV FIYLNSXSDA TDPSPLWDDA KKQMMTEVQS WPYKFPASED YPPSDQRGNV 300 KGRIQILDSF CEIRPKFYLN LXMSRYVSKD XVPGXGAYVG LAPPGDVGSW QRDCKGYQFW 360 TRADEKGYYS IQNIREGDYN LYAWCPGFIG DYRYDATISI TAGCDIDVDD IVYEPPRDGP 420 TLWEIGIPDR SAAEFYVPDP NPMYINKLYV NHPDRFRQYG LWERYQELYP DEDLIYSIGT 480 SDYTKDWFFA QVPRKKDEYT YQGTTWQIKF QLKHFSESDT LKLRIALATA NIAELQIRIN 540 DSKADPPLFT TGVIGNDNTI ARHGIHGLYR LYNIDIPGTL LVEGANTLFL TQPMSSSPLA 600 PFHGLMYDYI RLEGPSSSSS SSTT |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd10316 | RGL4_M | 3.0e-28 | 296 | 411 | 116 | + Middle domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle domain represented by this model and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
cd10317 | RGL4_C | 2.0e-49 | 423 | 613 | 193 | + C-terminal domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold. Both the middle and the C-terminal domain are putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
cd10320 | RGL4_N | 9.0e-50 | 6 | 262 | 296 | + N-terminal catalytic domain of rhamnogalacturonan lyase, a family 4 polysaccharide lyase. The rhamnogalacturonan lyase of the polysaccharide lyase family 4 (RGL4) is involved in the degradation of RG (rhamnogalacturonan) type-I, an important pectic plant cell wall polysaccharide, by cleaving the alpha-1,4 glycoside bond between L-rhamnose and D-galacturonic acids in the backbone of RG type-I through a beta-elimination reaction. RGL4 consists of three domains, an N-terminal catalytic domain, a middle domain with a FNIII type fold and a C-terminal domain with a jelly roll fold; the middle and C-terminal domains are both putative carbohydrate binding modules. There are two types of RG lyases, which both cleave the alpha-1,4 bonds of the RG-I main chain (RG chain) through the beta-elimination reaction, but belong to two structurally unrelated polysaccharide lyase (PL) families, 4 and 11. | ||
pfam06045 | Rhamnogal_lyase | 1.0e-100 | 1 | 203 | 203 | + Rhamnogalacturonate lyase family. Rhamnogalacturonate lyase (EC:4.2.2.-) degrades the rhamnogalacturonan I (RG-I) backbone of pectin. This family contains mainly members from plants, but also contains the plant pathogen Erwinia chrysanthemi. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CBI19298.1 | 0 | 1 | 623 | 1 | 652 | unnamed protein product [Vitis vinifera] |
RefSeq | XP_002285626.1 | 0 | 17 | 623 | 1 | 623 | PREDICTED: hypothetical protein isoform 1 [Vitis vinifera] |
RefSeq | XP_002301114.1 | 0 | 1 | 623 | 1 | 639 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002527353.1 | 0 | 1 | 619 | 1 | 637 | lyase, putative [Ricinus communis] |
RefSeq | XP_002527357.1 | 0 | 1 | 620 | 1 | 639 | lyase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1nkg_A | 0.001 | 312 | 612 | 237 | 506 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus |
PDB | 3njv_A | 0.001 | 312 | 612 | 237 | 506 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus |
PDB | 2xhn_B | 0.001 | 312 | 612 | 237 | 506 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus K150a Active Site Mutant |
PDB | 2xhn_A | 0.001 | 312 | 612 | 237 | 506 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus K150a Active Site Mutant |
PDB | 3njx_A | 0.001 | 312 | 612 | 237 | 506 | A Chain A, Rhamnogalacturonan Lyase From Aspergillus Aculeatus Mutant H210a |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
FY792769 | 285 | 298 | 582 | 0 |
EL409069 | 281 | 328 | 607 | 0 |
DY293973 | 361 | 1 | 320 | 0 |
DY922281 | 289 | 241 | 528 | 0 |
FF401051 | 260 | 325 | 584 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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