| Basic Information | |
|---|---|
| Species | Malus domestica |
| Cazyme ID | MDP0000283768 |
| Family | CBM43 |
| Protein Properties | Length: 1115 Molecular Weight: 124338 Isoelectric Point: 6.1412 |
| Chromosome | Chromosome/Scaffold: 006457144 Start: 25845 End: 39272 |
| Description | AMP-dependent synthetase and ligase family protein |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
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| Family | Start | End | Evalue |
| CBM43 | 334 | 416 | 2.3e-22 |
| WCVVAKGVSVNDEELGSAVNNACIAGNASGTCDALSPGKECYEPVSLTWHASFAFSSYWAKFRSQGATCHFNGLAEQTSLDPM | |||
| GH17 | 23 | 310 | 0 |
| IGINYGRLGNNLPSANRSIELLKAMNAGRVKLYDADLEILKLLSGTNLQVSIMVPNNEISTIXSDQTKADEWVRNNVLPYYPETMIRYLLIGNEVLSYNS SDQDRQMWYDLVPAMSKIKSSLKSQNITNIKVGTPLAMDALQSTFPPSKATFRADISDTVMAPMLRFLNRTRSFFFIDVYPFFPWSSNPGNISLDFALFK GNSTMEYTDPGTGLVYTNLLDQMLDSLIFAMTKLGYPNIRLLITETGWPSSGDIEQPGANIHNAATYNRNLIHRMVAKPPLGTPARPG | |||
| Full Sequence |
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| Protein Sequence Length: 1115 Download |
| MAPFLFFLFP LLGLSFAEIS SKIGINYGRL GNNLPSANRS IELLKAMNAG RVKLYDADLE 60 ILKLLSGTNL QVSIMVPNNE ISTIXSDQTK ADEWVRNNVL PYYPETMIRY LLIGNEVLSY 120 NSSDQDRQMW YDLVPAMSKI KSSLKSQNIT NIKVGTPLAM DALQSTFPPS KATFRADISD 180 TVMAPMLRFL NRTRSFFFID VYPFFPWSSN PGNISLDFAL FKGNSTMEYT DPGTGLVYTN 240 LLDQMLDSLI FAMTKLGYPN IRLLITETGW PSSGDIEQPG ANIHNAATYN RNLIHRMVAK 300 PPLGTPARPG KRVLEDYEPL PEEQNNRPYK GAVWCVVAKG VSVNDEELGS AVNNACIAGN 360 ASGTCDALSP GKECYEPVSL TWHASFAFSS YWAKFRSQGA TCHFNGLAEQ TSLDPMSQPP 420 SVKHSSFGLY DDTMPLLTAE DIQLQTEELQ IRRYSPFLES AFLNRNGASV SDEWQAVPDI 480 WRSSAERYGD RVALTDPYHD PPSSMTYKQL EEEILDFSEG LRVVGVKPED KIALFANNSC 540 RWLVADQGIM ATGAINVVRG SRSSVEELLQ IYDHSESVAL AVDSPELFNQ IAEAFSSKVV 600 TKFVILLWGE KSSLASEEKT PVFNYKEILD LGRESRKSML DFNDARGKYT YETINSDDVA 660 TLVYTSGTTG NPKGVMLTHR NLLHQIKNLW DVVPAEAGDR FLSMLPPWHA YERACEYFTL 720 TYGIEQVYTT VRNLKEDLLQ YQPNFIISVP LVYETLYSGI QKQISMSSTA RKFIALTFIR 780 ISLAYMELKR IYEGTYLTRN QKQPSYLASL YDWLWARVVA AILWPLHMLG KKLVYTKIHS 840 AVGISKAGIS GGGSLPSHVD KFFEAIGVKV QNGYGLTETS PVIAARRPNN NVLGSVGPPI 900 RHTEFKVVDL ETGEVLPPGS SGIINVRGPQ VMKGYYKNPA ATKQVLDEDG WLSTGDIGWI 960 APHHSTGRSR RCGGVIVLEG RAKDTIVLST GENVEPVELE EAAMRSSLIQ QIVVVGQDQR 1020 RLGAIIVPNK EEVLLAAKKL SIVDVNASEL SMDKMKNLVY EDLRRWTTGC SFQIGPILIV 1080 DEPFTIDSGL MTPTMKIRRD RVVAQFKEQI NNLYK |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| cd05907 | VL_LC_FACS_like | 2.0e-20 | 500 | 586 | 88 | + Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases. This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions. | ||
| pfam00332 | Glyco_hydro_17 | 5.0e-90 | 23 | 311 | 290 | + Glycosyl hydrolases family 17. | ||
| cd05927 | LC-FACS_euk | 6.0e-95 | 505 | 1114 | 625 | + Eukaryotic long-chain fatty acid CoA synthetase (LC-FACS). The members of this family are eukaryotic fatty acid CoA synthetases that activate fatty acids with chain lengths of 12 to 20. LC-FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. This is a required step before free fatty acids can participate in most catabolic and anabolic reactions. Organisms tend to have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. | ||
| cd05907 | VL_LC_FACS_like | 5.0e-130 | 656 | 1102 | 457 | + Long-chain fatty acid CoA synthetases and Bubblegum-like very long-chain fatty acid CoA synthetases. This family includes long-chain fatty acid (C12-C20) CoA synthetases and Bubblegum-like very long-chain (>C20) fatty acid CoA synthetases. FACS catalyzes the formation of fatty acyl-CoA in a two-step reaction: the formation of a fatty acyl-AMP molecule as an intermediate, and the formation of a fatty acyl-CoA. Eukaryotes generally have multiple isoforms of LC-FACS genes with multiple splice variants. For example, nine genes are found in Arabidopsis and six genes are expressed in mammalian cells. Drosophila melanogaster mutant bubblegum (BGM) have elevated levels of very-long-chain fatty acids (VLCFA) caused by a defective gene later named bubblegum. The human homolog (hsBG) of bubblegum has been characterized as a very long chain fatty acid CoA synthetase that functions specifically in the brain; hsBG may play a central role in brain VLCFA metabolism and myelinogenesis. Free fatty acids must be "activated" to their CoA thioesters before participating in most catabolic and anabolic reactions. | ||
| COG1022 | FAA1 | 2.0e-158 | 476 | 1115 | 651 | + Long-chain acyl-CoA synthetases (AMP-forming) [Lipid metabolism] | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0003824 | catalytic activity |
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
| GO:0005975 | carbohydrate metabolic process |
| GO:0008152 | metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAM28629.1 | 0 | 445 | 1115 | 51 | 722 | AF503771_1 acyl-CoA synthetase-like protein [Arabidopsis thaliana] |
| EMBL | CBI39044.1 | 0 | 443 | 1114 | 56 | 729 | unnamed protein product [Vitis vinifera] |
| RefSeq | XP_002263501.1 | 0 | 446 | 1114 | 20 | 690 | PREDICTED: hypothetical protein [Vitis vinifera] |
| RefSeq | XP_002298670.1 | 0 | 442 | 1115 | 19 | 694 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002328916.1 | 0 | 420 | 1115 | 11 | 706 | predicted protein [Populus trichocarpa] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2cyg_A | 0 | 23 | 313 | 1 | 276 | A Chain A, Crystal Structure At 1.45- Resolution Of The Major Allergen Endo-Beta-1,3-Glucanase Of Banana As A Molecular Basis For The Latex-Fruit Syndrome |
| PDB | 1ghs_B | 0 | 23 | 317 | 1 | 272 | A Chain A, The Three-Dimensional Structures Of Two Plant Beta-Glucan Endohydrolases With Distinct Substrate Specificities |
| PDB | 1ghs_A | 0 | 23 | 317 | 1 | 272 | A Chain A, The Three-Dimensional Structures Of Two Plant Beta-Glucan Endohydrolases With Distinct Substrate Specificities |
| PDB | 3f55_D | 4.06377e-44 | 22 | 317 | 1 | 282 | A Chain A, The Three-Dimensional Structures Of Two Plant Beta-Glucan Endohydrolases With Distinct Substrate Specificities |
| PDB | 3f55_C | 4.06377e-44 | 22 | 317 | 1 | 282 | A Chain A, The Three-Dimensional Structures Of Two Plant Beta-Glucan Endohydrolases With Distinct Substrate Specificities |
