Basic Information | |
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Species | Populus trichocarpa |
Cazyme ID | Potri.010G092900.4 |
Family | CBM45 |
Protein Properties | Length: 903 Molecular Weight: 101078 Isoelectric Point: 6.7301 |
Chromosome | Chromosome/Scaffold: 10 Start: 11582343 End: 11593518 |
Description | alpha-amylase-like 3 |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
CBM45 | 316 | 392 | 4e-23 |
VHWGVCRDDAKKWEIPAAPHPPETTVFKNKALRTVLQAKEDGNGRSGSFTLDEDLVGFLFVLKLNDSTWLNCMGNDF | |||
CBM45 | 140 | 223 | 4.9e-27 |
LHWGVSYLDDTGSEWDQPPENMRPPGSIPVKDYAIETPLKKASEGDKFHQVKIGIDPKSPVAALNFVLKDEETGVWYQHKGRDF | |||
GH13 | 532 | 822 | 2.1e-38 |
KQKIEEISSLGFTVVWLPPPTESVSPEGYMPKDLYNLNSRYGNIDELKDLVKRFHGKGVKVLGDAVLNHRCAHYKNGNGVWNIFGGRLNWDDRAVVADDP HFQGRGNKSSGDNFHAAPNIDHSQEFVRKDLKEWLLWLRKEIGYDGWRLDFVRGFWGGYVKDYLDASEPYFAVGEYWDSLSYTYGELDHDQDAHRQRIVD WINATSGTAGAFDVTTKGILHTTLERCEYWRLSDQKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPSGKEMQGYAYILTHPGTPAVFY |
Full Sequence |
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Protein Sequence Length: 903 Download |
MSAVSVEPFL RYSRFEKPGY ARSRRKAAIT VTTAALIKSP SSFNCCCSLI PRRKLLSNAS 60 YFPFLDLHRV KTHTARASST DTALVESTND VVFKETFPLS RTEMTEGKIF VRLDQSKAKE 120 KEDQWQQLTV GCSLPGKWIL HWGVSYLDDT GSEWDQPPEN MRPPGSIPVK DYAIETPLKK 180 ASEGDKFHQV KIGIDPKSPV AALNFVLKDE ETGVWYQHKG RDFKVPLVDC LLDSGGGNVI 240 GAKGGFNALA SEGKDSSSRS KDPKQETRKV EGFYEELPIA KFAVIENSVT VSVIKCLKTA 300 KNLLYLVTDL PGEVVVHWGV CRDDAKKWEI PAAPHPPETT VFKNKALRTV LQAKEDGNGR 360 SGSFTLDEDL VGFLFVLKLN DSTWLNCMGN DFYIALPISS SIPALSGAGQ SEVAPVSENT 420 VGADQEVSHA IYTDGIINEI RSLVSDFSSE KRQKTKTKEA QESILQEIEK LAAEAYSIFR 480 SSIPTFLDET ALESEATEAP KICSGTGTGH EILLQGFNWE SHKLGHWYME LKQKIEEISS 540 LGFTVVWLPP PTESVSPEGY MPKDLYNLNS RYGNIDELKD LVKRFHGKGV KVLGDAVLNH 600 RCAHYKNGNG VWNIFGGRLN WDDRAVVADD PHFQGRGNKS SGDNFHAAPN IDHSQEFVRK 660 DLKEWLLWLR KEIGYDGWRL DFVRGFWGGY VKDYLDASEP YFAVGEYWDS LSYTYGELDH 720 DQDAHRQRIV DWINATSGTA GAFDVTTKGI LHTTLERCEY WRLSDQKGKP PGVVGWWPSR 780 AVTFIENHDT GSTQGHWRFP SGKEMQGYAY ILTHPGTPAV FYDHIFSHYQ SEIAALISLR 840 NRNKIHCRST VKITKAERDV YAAIIDEKVA VKIGPGHYEP PSGPYSWSSK IEGRNYKVWE 900 AS* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PRK09441 | PRK09441 | 1.0e-49 | 508 | 842 | 420 | + cytoplasmic alpha-amylase; Reviewed | ||
PLN00196 | PLN00196 | 3.0e-143 | 511 | 900 | 403 | + alpha-amylase; Provisional | ||
cd11314 | AmyAc_arch_bac_plant_AmyA | 6.0e-166 | 512 | 851 | 343 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN02361 | PLN02361 | 6.0e-170 | 509 | 900 | 398 | + alpha-amylase | ||
PLN02784 | PLN02784 | 0 | 1 | 902 | 923 | + alpha-amylase |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004556 | alpha-amylase activity |
GO:0005509 | calcium ion binding |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAX33231.1 | 0 | 1 | 902 | 1 | 901 | plastid alpha-amylase [Malus x domestica] |
EMBL | CBI32016.1 | 0 | 1 | 902 | 1 | 885 | unnamed protein product [Vitis vinifera] |
RefSeq | NP_564977.1 | 0 | 1 | 902 | 1 | 887 | AMY3 (ALPHA-AMYLASE-LIKE 3); alpha-amylase [Arabidopsis thaliana] |
RefSeq | XP_002270049.1 | 0 | 1 | 902 | 1 | 901 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002520134.1 | 0 | 1 | 902 | 1 | 900 | alpha-amylase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2qpu_C | 0 | 510 | 900 | 1 | 403 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
PDB | 2qpu_B | 0 | 510 | 900 | 1 | 403 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
PDB | 2qpu_A | 0 | 510 | 900 | 1 | 403 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
PDB | 3bsg_A | 0 | 510 | 900 | 1 | 403 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
PDB | 1rpk_A | 0 | 510 | 900 | 1 | 403 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
starch degradation I | RXN-1823 | EC-3.2.1.1 | α-amylase |
starch degradation I | RXN-1825 | EC-3.2.1.1 | α-amylase |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
EG631183 | 927 | 1 | 903 | 0 |
HO826981 | 405 | 499 | 903 | 0 |
HO811991 | 299 | 605 | 903 | 0 |
ES805448 | 328 | 469 | 795 | 0 |
HO826981 | 27 | 467 | 493 | 0.22 |
Sequence Alignments (This image is cropped. Click for full image.) |
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