PlantCAZyme

Basic Information
Species	Fragaria vesca
Cazyme ID	mrna09491.1-v1.0-hybrid
Family	GH1
Protein Properties	Length: 1443 Molecular Weight: 163174 Isoelectric Point: 5.346
Chromosome	Chromosome/Scaffold: 5 Start: 10627819 End: 10638881
Description	alpha-N-acetylglucosaminidase family / NAGLU family
View CDS

Signature Domain Download full data set without filtering

Family	Start	End	Evalue
GH89	106	845	0
PQILISGVTGVEILAGLHWYLKNWCGSHISWDKTGGAQLFSVPKAGLLSCVGDGGILVQRPVPWNYYQNAVTSSYSLAWWDWIRWEKEIDMMALQGINLP LAFTGQEAIWQKVFQKFNISKSDLDDFFGGPAFLSWSRMGNLHGWGGPLPQSWLDQQLILQKRILDRMYELGMTPVLPAFSGNVPAALKTIYPAAKITQL GNWFSVKSDPRWTCTYLLDATDPLFVEIGKTFIEEQLKEYGRTSHIYNCDTFDENTPPVDDPEYISALGKTIFKGLQSGDKDGVWLMQGWLFSYDPFWRP AQMKALLHSVPAGRMVVLDLFAEVKPIWTTSEQFYGVPYIWKVIAPFLDWELAFFGIHYRCMLHNFAGNVEMYGVLDAIASGPIDAWTSENSTMVGVGMS MEGIEQNPVVYDLMSEMAFQQNKVDVKDWINLYSTRRYGRAVPLVQDAWSILRHTTYNCTDGAYRALRSLNSSTLQDCTAGSCSKKHAYTDKNRDVIVAF PDVDPSFIARPPQGYYQNEKSLVSRRAELLEEVTDSFERPHLWYSTSEVVHALELFIASGDELSGSNTYRYDLVDLTRQALAKYANELFLKVIEAYHLND TLEVVGLSQKFLELVEDMDTLLACHDGFLLGPWLESAKKLAQDKEQEIQFEWNARTQITMWFDNTEEEASLLRDYGNKYWSGLLRDYYGPRAAIYFKYLI KSLDEGSDFDLKNWRREWIKLTNDWQSSRNTFPVKSTGNA
GH1	1116	1440	0
CISQEGIDHYNNVINEIVRNGIEPFVTLLHFDLPQASEDKNDFKDYSELCSKEFGDRVRYWFTINEPTVSVVYAYELEIAPPGRCSFKEGFCVFGAPPPG PCHAQQKGEIGAVLVAPYLIPYSDSPEDIAATERSYDFNLGWFLEPFVYGEYPKSMKVVVKERLPSFSAEEKSLIKGSLDFVGMNYYISSYVKSKQPSPT EEPRYTIEVAAEQTRQWKSAKRLEGLMVYIKEKYQNPKIYISENGLPMSRKDNSSLSERLKDSDRISYIAKHLYSQSGVNIRGYFYWSLFEWGMGLFESF GLHYDNLKRIPKQSAEWFQRFLQRN

Full Sequence
Protein Sequence Length: 1443 Download
MASATSPAIS TVTIFTLFFF FTLCVLSSSS PSTIGVGYIS NVLELQDRER APPYVQVAAA 60 RGVLHRLLPS HSSSFDFQIV SKEQCGGASC FMIKNHPSFR RRGIDPQILI SGVTGVEILA 120 GLHWYLKNWC GSHISWDKTG GAQLFSVPKA GLLSCVGDGG ILVQRPVPWN YYQNAVTSSY 180 SLAWWDWIRW EKEIDMMALQ GINLPLAFTG QEAIWQKVFQ KFNISKSDLD DFFGGPAFLS 240 WSRMGNLHGW GGPLPQSWLD QQLILQKRIL DRMYELGMTP VLPAFSGNVP AALKTIYPAA 300 KITQLGNWFS VKSDPRWTCT YLLDATDPLF VEIGKTFIEE QLKEYGRTSH IYNCDTFDEN 360 TPPVDDPEYI SALGKTIFKG LQSGDKDGVW LMQGWLFSYD PFWRPAQMKA LLHSVPAGRM 420 VVLDLFAEVK PIWTTSEQFY GVPYIWKVIA PFLDWELAFF GIHYRCMLHN FAGNVEMYGV 480 LDAIASGPID AWTSENSTMV GVGMSMEGIE QNPVVYDLMS EMAFQQNKVD VKDWINLYST 540 RRYGRAVPLV QDAWSILRHT TYNCTDGAYR ALRSLNSSTL QDCTAGSCSK KHAYTDKNRD 600 VIVAFPDVDP SFIARPPQGY YQNEKSLVSR RAELLEEVTD SFERPHLWYS TSEVVHALEL 660 FIASGDELSG SNTYRYDLVD LTRQALAKYA NELFLKVIEA YHLNDTLEVV GLSQKFLELV 720 EDMDTLLACH DGFLLGPWLE SAKKLAQDKE QEIQFEWNAR TQITMWFDNT EEEASLLRDY 780 GNKYWSGLLR DYYGPRAAIY FKYLIKSLDE GSDFDLKNWR REWIKLTNDW QSSRNTFPVK 840 STGNASLVVF MPENGALVLT NGNIPEHDEK FRGAAKNNWT SIILVDGRSP ILLSSLTMDH 900 GDKKQSSTYG ETTSWADQWD YTNNNSSYPM DVKHGEQGTA AKCKQKVGEG LVKTKAVAST 960 GAKKVKDGAS SGFQWIKDKS VKAQETLLCT WILMEKNVVE SIWPMGNLSS ECMPSSFDIH 1020 RELLSFRLLI LIKVDDLGAV LGNFTAGGLT FQVSLNSSSF PREELDIKRS DIPSEFYFGV 1080 GTSAAQTEGS AKEGGRGSSI WDHFVEQNLD GSLSGCISQE GIDHYNNVIN EIVRNGIEPF 1140 VTLLHFDLPQ ASEDKNDFKD YSELCSKEFG DRVRYWFTIN EPTVSVVYAY ELEIAPPGRC 1200 SFKEGFCVFG APPPGPCHAQ QKGEIGAVLV APYLIPYSDS PEDIAATERS YDFNLGWFLE 1260 PFVYGEYPKS MKVVVKERLP SFSAEEKSLI KGSLDFVGMN YYISSYVKSK QPSPTEEPRY 1320 TIEVAAEQTR QWKSAKRLEG LMVYIKEKYQ NPKIYISENG LPMSRKDNSS LSERLKDSDR 1380 ISYIAKHLYS QSGVNIRGYF YWSLFEWGMG LFESFGLHYD NLKRIPKQSA EWFQRFLQRN 1440 DC*

Full Sequence

Protein Sequence Length: 1443 Download

MASATSPAIS TVTIFTLFFF FTLCVLSSSS PSTIGVGYIS NVLELQDRER APPYVQVAAA    60
RGVLHRLLPS HSSSFDFQIV SKEQCGGASC FMIKNHPSFR RRGIDPQILI SGVTGVEILA    120
GLHWYLKNWC GSHISWDKTG GAQLFSVPKA GLLSCVGDGG ILVQRPVPWN YYQNAVTSSY    180
SLAWWDWIRW EKEIDMMALQ GINLPLAFTG QEAIWQKVFQ KFNISKSDLD DFFGGPAFLS    240
WSRMGNLHGW GGPLPQSWLD QQLILQKRIL DRMYELGMTP VLPAFSGNVP AALKTIYPAA    300
KITQLGNWFS VKSDPRWTCT YLLDATDPLF VEIGKTFIEE QLKEYGRTSH IYNCDTFDEN    360
TPPVDDPEYI SALGKTIFKG LQSGDKDGVW LMQGWLFSYD PFWRPAQMKA LLHSVPAGRM    420
VVLDLFAEVK PIWTTSEQFY GVPYIWKVIA PFLDWELAFF GIHYRCMLHN FAGNVEMYGV    480
LDAIASGPID AWTSENSTMV GVGMSMEGIE QNPVVYDLMS EMAFQQNKVD VKDWINLYST    540
RRYGRAVPLV QDAWSILRHT TYNCTDGAYR ALRSLNSSTL QDCTAGSCSK KHAYTDKNRD    600
VIVAFPDVDP SFIARPPQGY YQNEKSLVSR RAELLEEVTD SFERPHLWYS TSEVVHALEL    660
FIASGDELSG SNTYRYDLVD LTRQALAKYA NELFLKVIEA YHLNDTLEVV GLSQKFLELV    720
EDMDTLLACH DGFLLGPWLE SAKKLAQDKE QEIQFEWNAR TQITMWFDNT EEEASLLRDY    780
GNKYWSGLLR DYYGPRAAIY FKYLIKSLDE GSDFDLKNWR REWIKLTNDW QSSRNTFPVK    840
STGNASLVVF MPENGALVLT NGNIPEHDEK FRGAAKNNWT SIILVDGRSP ILLSSLTMDH    900
GDKKQSSTYG ETTSWADQWD YTNNNSSYPM DVKHGEQGTA AKCKQKVGEG LVKTKAVAST    960
GAKKVKDGAS SGFQWIKDKS VKAQETLLCT WILMEKNVVE SIWPMGNLSS ECMPSSFDIH    1020
RELLSFRLLI LIKVDDLGAV LGNFTAGGLT FQVSLNSSSF PREELDIKRS DIPSEFYFGV    1080
GTSAAQTEGS AKEGGRGSSI WDHFVEQNLD GSLSGCISQE GIDHYNNVIN EIVRNGIEPF    1140
VTLLHFDLPQ ASEDKNDFKD YSELCSKEFG DRVRYWFTIN EPTVSVVYAY ELEIAPPGRC    1200
SFKEGFCVFG APPPGPCHAQ QKGEIGAVLV APYLIPYSDS PEDIAATERS YDFNLGWFLE    1260
PFVYGEYPKS MKVVVKERLP SFSAEEKSLI KGSLDFVGMN YYISSYVKSK QPSPTEEPRY    1320
TIEVAAEQTR QWKSAKRLEG LMVYIKEKYQ NPKIYISENG LPMSRKDNSS LSERLKDSDR    1380
ISYIAKHLYS QSGVNIRGYF YWSLFEWGMG LFESFGLHYD NLKRIPKQSA EWFQRFLQRN    1440
DC* 

Functional Domains Download unfiltered results here

Cdd ID	Domain	E-Value	Start	End	Length	Domain Description
PLN02849	PLN02849	6.0e-76	1059	1442	473	+ beta-glucosidase
TIGR03356	BGL	6.0e-81	1073	1433	433	+ beta-galactosidase.
pfam12972	NAGLU_C	8.0e-85	533	845	313	+ Alpha-N-acetylglucosaminidase (NAGLU) C-terminal domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This C-terminal domain has an all alpha helical fold.
pfam00232	Glyco_hydro_1	3.0e-99	1073	1440	453	+ Glycosyl hydrolase family 1.
pfam05089	NAGLU	2.0e-177	171	528	358	+ Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This central domain has a tim barrel fold.

Gene Ontology
GO Term	Description
GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds
GO:0005975	carbohydrate metabolic process

Annotations - PDB Download unfiltered results here
Source	Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
PDB	2vcc_A	0	75	834	190	850	A Chain A, Structure Of The Thermostable Pectate Lyase Pl 47
PDB	2vcb_A	0	75	834	190	850	A Chain A, Structure Of The Thermostable Pectate Lyase Pl 47
PDB	2vca_A	0	75	834	190	850	A Chain A, Structure Of The Thermostable Pectate Lyase Pl 47
PDB	2vc9_A	0	75	834	190	850	A Chain A, Family 89 Glycoside Hydrolase From Clostridium Perfringens In Complex With 2-Acetamido-1,2-Dideoxynojirmycin
PDB	4a4a_A	0	75	834	213	873	A Chain A, Cpgh89 (E483q, E601q), From Clostridium Perfringens, In Complex With Its Substrate Glcnac-Alpha-1,4-Galactose

Signal Peptide

Cleavage Site
27

CAZyme Information