Basic Information | |
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Species | Citrus sinensis |
Cazyme ID | orange1.1g003698m |
Family | CBM45 |
Protein Properties | Length: 803 Molecular Weight: 90932.3 Isoelectric Point: 5.4037 |
Chromosome | Chromosome/Scaffold: 00003 Start: 2697619 End: 2704871 |
Description | alpha-amylase-like 3 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
CBM45 | 219 | 295 | 1.2e-23 |
VHWGVCRDDSKNWEIPAEPYPPETIVFKNKALRTLLQPKEGGKGCSRLFTVDEEFAGFLFVLKLNENTWLKCMENDF | |||
CBM45 | 28 | 111 | 1.7e-27 |
LHWGVSFVGDNGSEWDQPPKKMRPPGSVSIKDYAIETPLKKLAEGDVFDQVNIDFDTRSDIAAINFVLKDEETGAWYQHRGRDF | |||
GH13 | 435 | 722 | 1.2e-36 |
ATELSSLGFSVIWLPPPTESVSPEGYMPRDLYNLSSRYGNIDELKDVVNKFHDVGMKILGDVVLNHRCAHYQNQNGVWNIFGGRLNWDDRAVVADDPHFQ GRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWLRNEIGYDGWRLDFVRGFWGGYVKDYLEATEPYFAVGEYWDSLSYTYGEMDHNQDAHRQRIIDWIN AASGTAGAFDVTTKGILHSALDRCEYWRLSDEKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRFPGGREMQGYAYILTHPGTPSVFY |
Full Sequence |
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Protein Sequence Length: 803 Download |
MFVRLQKGKD EKNWQLSVGC NIPGKWILHW GVSFVGDNGS EWDQPPKKMR PPGSVSIKDY 60 AIETPLKKLA EGDVFDQVNI DFDTRSDIAA INFVLKDEET GAWYQHRGRD FKVPLVDYLQ 120 HDGNVIGTKS TFGLWPGALG QLSKMILKAD TSQSGIQDSS SESCELKQEN KHLEGFYEEL 180 PIVKEIIIEN TVSVSVRKCP ETAKTLLNLE TDLTGDVVVH WGVCRDDSKN WEIPAEPYPP 240 ETIVFKNKAL RTLLQPKEGG KGCSRLFTVD EEFAGFLFVL KLNENTWLKC MENDFYIPLT 300 SSSCLPAESV QEMLIPGKAE EATQEVSQTA YTAGIIKEIR NLVSDFSSDI SRKTKSKEAQ 360 KSILLEIEKL AAEAYSIFRT TAPTFFEEAA VELEESKPPA KISPGTGTGF EILCQGFNWE 420 SHKSGRWYME LKEKATELSS LGFSVIWLPP PTESVSPEGY MPRDLYNLSS RYGNIDELKD 480 VVNKFHDVGM KILGDVVLNH RCAHYQNQNG VWNIFGGRLN WDDRAVVADD PHFQGRGNKS 540 SGDNFHAAPN IDHSQDFVRK DIKEWLCWLR NEIGYDGWRL DFVRGFWGGY VKDYLEATEP 600 YFAVGEYWDS LSYTYGEMDH NQDAHRQRII DWINAASGTA GAFDVTTKGI LHSALDRCEY 660 WRLSDEKGKP PGVVGWWPSR AVTFIENHDT GSTQGHWRFP GGREMQGYAY ILTHPGTPSV 720 FYDHIFSHYR QEIEALLSVR KRNKIHCRSR VEIVKAERDV YAAIIDEKVA MKLGPGHYEP 780 PSGSQNWSFV TEGRDYKVWE AA* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PRK09441 | PRK09441 | 5.0e-49 | 411 | 742 | 416 | + cytoplasmic alpha-amylase; Reviewed | ||
PLN00196 | PLN00196 | 1.0e-135 | 411 | 802 | 409 | + alpha-amylase; Provisional | ||
cd11314 | AmyAc_arch_bac_plant_AmyA | 1.0e-163 | 412 | 751 | 343 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN02361 | PLN02361 | 2.0e-168 | 409 | 800 | 398 | + alpha-amylase | ||
PLN02784 | PLN02784 | 0 | 1 | 802 | 803 | + alpha-amylase |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004556 | alpha-amylase activity |
GO:0005509 | calcium ion binding |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAX33231.1 | 0 | 1 | 802 | 96 | 901 | plastid alpha-amylase [Malus x domestica] |
EMBL | CBI32016.1 | 0 | 3 | 802 | 92 | 885 | unnamed protein product [Vitis vinifera] |
RefSeq | XP_002520134.1 | 0 | 1 | 802 | 96 | 900 | alpha-amylase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2qpu_C | 0 | 411 | 800 | 2 | 403 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
PDB | 2qpu_B | 0 | 411 | 800 | 2 | 403 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
PDB | 2qpu_A | 0 | 411 | 800 | 2 | 403 | A Chain A, Sugar Tongs Mutant S378p In Complex With Acarbose |
PDB | 3bsg_A | 0 | 411 | 800 | 2 | 403 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
PDB | 1rpk_A | 0 | 411 | 800 | 2 | 403 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
EG631183 | 807 | 1 | 803 | 0 |
HO826981 | 407 | 397 | 803 | 0 |
DR932783 | 288 | 411 | 698 | 0 |
HO826981 | 30 | 366 | 395 | 0.64 |
EG631183 | 137 | 174 | 299 | 2.3 |
Sequence Alignments (This image is cropped. Click for full image.) |
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