LOCUS       ACXX02000001            2518 bp    DNA     linear   BCT 14-MAR-2011
DEFINITION  Ruminiclostridium papyrosolvens DSM 2782 ctg65, whole genome shotgun
            sequence.
ACCESSION   ACXX02000001
VERSION     ACXX02000001.1
DBLINK      BioProject: PRJNA33587
            BioSample: SAMN00002727
KEYWORDS    WGS; GSC:MIGS:2.1; NONCONTIGUOUS_FINISHED.
SOURCE      Ruminiclostridium papyrosolvens DSM 2782
  ORGANISM  Ruminiclostridium papyrosolvens DSM 2782
            Bacteria; Firmicutes; Clostridia; Clostridiales;
            Hungateiclostridiaceae; Ruminiclostridium.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L.,
            Pitluck,S., Misra,M., Chertkov,O., Detter,J.C., Han,C., Tapia,R.,
            Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Mouttaki,H.,
            He,Z., Zhou,J., Hemme,C.L. and Woyke,T.
  TITLE     The Non-contiguous Finished genome of Clostridium papyrosolvens
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H.,
            Bruce,D., Goodwin,L., Pitluck,S., Larimer,F., Land,M.L.,
            Mouttaki,H., He,Z., Zhou,J. and Hemme,C.L.
  CONSRTM   US DOE Joint Genome Institute (JGI-PGF)
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUL-2009) US DOE Joint Genome Institute, 2800 Mitchell
            Drive B310, Walnut Creek, CA 94598-1698, USA
REFERENCE   3  (bases 1 to 2518)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Goodwin,L.,
            Pitluck,S., Misra,M., Chertkov,O., Detter,J.C., Han,C., Tapia,R.,
            Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Pagani,I., Mouttaki,H.,
            He,Z., Zhou,J., Hemme,C.L. and Woyke,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2011) US DOE Joint Genome Institute, 2800 Mitchell
            Drive B310, Walnut Creek, CA 94598-1698, USA
COMMENT     ##MIGS-Data-START##
            investigation_type       :: bacteria_archaea
            project_name             :: Clostridium papyrosolvens DSM 2782
            collection_date          :: Missing
            lat_lon                  :: 57.162143 -2.719417
            depth                    :: Missing
            alt_elev                 :: Missing
            country                  :: United Kingdom
            environment              :: Mud
            num_replicons            :: Missing
            ref_biomaterial          :: DSM 2782, ATCC 35413, NCIB 11394
            biotic_relationship      :: Free living
            trophic_level            :: Chemoorganotroph
            rel_to_oxygen            :: Anaerobe
            isol_growth_condt        :: Missing
            sequencing_meth          :: WGS
            assembly                 :: Newbler v. 2.3 (pre-release)
            finishing_strategy       :: Finished
            GOLD Stamp ID            :: Gi02584
            Type Strain              :: Yes
            Greengenes ID            :: 15957
            Isolation Site           :: estuarine sediment, River Don,
            Source of Isolate        :: Christopher L. Hemme (hemmecl@ou.edu)
            Cell Shape               :: Rod-shaped
            Motility                 :: Motile
            Sporulation              :: Sporulating
            Temperature Range        :: Mesophile
            Temperature Optimum      :: 25C
            Gram Staining            :: Gram-
            ##MIGS-Data-END##
            ##Genome-Assembly-Data-START##
            Finishing Goal           :: Noncontiguous Finished
            Current Finishing Status :: Noncontiguous Finished
            Assembly Method          :: Newbler v. 2.3
            Genome Coverage          :: 30x
            Sequencing Technology    :: 454; Illumina
            ##Genome-Assembly-Data-END##
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 4085558
            Source DNA and Organism available from Christopher L. Hemme
            (hemmecl@ou.edu)
            Contacts: Christopher L. Hemme (hemmecl@ou.edu)
            David Bruce (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            Aberdeenshire, Scotland
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Ruminiclostridium papyrosolvens DSM 2782"
                     /mol_type="genomic DNA"
                     /submitter_seqid="ctg65"
                     /strain="DSM 2782"
                     /isolation_source="estuarine sediment from River Don"
                     /type_material="type strain of Ruminiclostridium
                     papyrosolvens"
                     /db_xref="taxon:588581"
                     /country="United Kingdom: Aberdeenshire, Scotland"
     gene            1..1428
                     /locus_tag="Cpap_3849"
     CDS             1..1428
                     /locus_tag="Cpap_3849"
                     /inference="protein motif:PFAM:PF00150"
                     /note="PFAM: glycoside hydrolase family 5; Dockerin type 1;
                     KEGG: cce:Ccel_1099 glycoside hydrolase family 5"
                     /codon_start=1
                     /transl_table=11
                     /product="GH5|GH5_4|CBM79"
                     /protein_id="EGD49416.1"
                     /db_xref="GO:0005524"
                     /db_xref="InterPro:IPR001547"
                     /db_xref="InterPro:IPR002105"
                     /db_xref="InterPro:IPR018087"
                     /db_xref="InterPro:IPR018242"
                     /db_xref="InterPro:IPR018247"
                     /translation="MKKTTAFLLCFLMIFTALLPLQNANAYDASLIPNLQIQQKNVPGN
                     DAMNFVKKLRLGWNLGNTFDANNGTNITNELDYETSWSGIKTTKQMIDTIKQKGFNTVR
                     IPVSWFPHLSGSDYKISDVWMNRVQEVVNYCIDNKMYVILNTHHDIDKAKGYFPSSQYL
                     TSSKKFITSVWAQVAAKFANYDEHLIFEGMNEPRLVGHANEWWPDLKNSDVLDSINCIN
                     QLNQDFVNTVRATGGKNASRYLMCPGYVASPDGATNDYFKMPNDISSNNNKLIVSVHAY
                     CPWVFAGQSMSSGGVSTWNINDSKDQSEVTWFMDNIYNKYTSRGIPVIIGECGAVDKNN
                     LKTRVEYMSYYVAQAKARGILCVLWDNNNFSGTGELFGFLDRRNCQFKFPEIMDGMVKY
                     AFEAQNDPDPVIVYGDINNDGYVDSLDFAGLKKYLLNADHAYVKNLDVNLDNEVNAFDL
                     VVLKKYLLGMVTILPSN"
     sig_peptide     1..81
                     /locus_tag="Cpap_3849"
                     /note="Signal predicted by SignalP 3.0 HMM (Signal peptide
                     probabilty 0.999) with cleavage site probability 0.981 at
                     residue 27"
     gene            1808..2518
                     /locus_tag="Cpap_3850"
     CDS             1808..2518
                     /locus_tag="Cpap_3850"
                     /inference="similar to AA sequence:KEGG:Cthe_1400"
                     /note="KEGG: cth:Cthe_1400 glycosyl hydrolase 53"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="EGD49417.1"
                     /db_xref="InterPro:IPR018247"
                     /translation="MKGLRKSKAVFAMALVLALLITCGAFPVAAQEGGLIIGDANGDGL
                     VNVTDAAILNLYVSGYTNTFPYDMADYTMDVNDDGYINRQDFDLLNKFISGSLASLPSA
                     DILRNMPQTVFQYSYVNGAWGYRNIRLSIDKQGNIYTSGNITPNVDPSTIKVPKIEIES
                     MYLDLYLASQGTITNPQNAACDMGINVYGGNIVKNGALQYVFLKQYGDVDQENMSPYTQ
                     TLVDWLDGYVDMIY"
     sig_peptide     1808..1900
                     /locus_tag="Cpap_3850"
                     /note="Signal predicted by SignalP 3.0 HMM (Signal peptide
                     probabilty 1.000) with cleavage site probability 0.997 at
                     residue 31"
ORIGIN
        1 atgaaaaaaa caacagcctt tttattatgt tttctaatga tttttacagc attattgcca
       61 ctgcaaaatg ctaatgctta tgatgcttca cttatcccga atcttcagat tcagcaaaag
      121 aacgttcccg gtaatgatgc aatgaatttt gtaaagaaat taaggcttgg atggaatctg
      181 ggtaatacat ttgatgcaaa taacggtaca aatattacta atgaattgga ttatgaaaca
      241 tcctggagcg gtatcaaaac aactaagcag atgattgata ccataaagca aaaaggattc
      301 aatacagttc gtattcctgt atcctggttt ccacacttaa gtggctcaga ttacaaaatc
      361 agtgatgtgt ggatgaatcg tgttcaagaa gtagtaaatt attgtataga taataaaatg
      421 tatgtcattt taaacacaca tcatgacatt gacaaagcaa aaggttattt cccaagtagt
      481 caatatttga caagctctaa gaaatttata accagtgtat gggcacaggt tgctgctaag
      541 tttgcaaact atgatgagca tcttattttt gaaggaatga acgagcctcg tcttgtagga
      601 catgctaatg agtggtggcc tgatttaaaa aattccgatg tacttgattc tattaattgt
      661 attaatcaac ttaatcagga ttttgttaat acagtacgtg caacaggtgg aaaaaatgca
      721 agcagatatt taatgtgtcc gggatatgtt gcatctcctg atggagcaac aaatgattac
      781 tttaaaatgc caaatgatat ttcaagtaat aataataaat taattgtatc cgtacatgca
      841 tactgtcctt gggtttttgc aggacagtca atgtccagtg gaggagtaag tacttggaat
      901 ataaatgatt caaaagatca aagtgaagtt acttggttta tggataatat ttataataaa
      961 tatacaagca ggggcattcc tgtaataatc ggtgaatgtg gagcagtaga caagaacaat
     1021 ctgaagacaa gagtagaata tatgtcctat tatgttgcac aagccaaagc acgtggtata
     1081 ttatgcgtat tgtgggataa caataatttc tcaggtaccg gtgaattatt tggtttctta
     1141 gatagaagaa actgtcagtt caagttccct gaaatcatgg atggaatggt gaaatatgct
     1201 tttgaagcac agaatgatcc tgaccctgta attgtatatg gagatattaa caacgatgga
     1261 tatgttgact cacttgattt tgcaggctta aagaaatatc ttttgaatgc tgaccatgct
     1321 tatgtaaaga atttggatgt taatctcgac aatgaagtaa atgcatttga ccttgtagtt
     1381 ttgaaaaaat atctccttgg tatggtaacc atacttccaa gcaactaaac aatagtatta
     1441 aaaaaagtat cattcaaaag gtgtaaaagc ctgatgaatg atactttttt cataaaatag
     1501 taaatatttc cctatattct ctataagcct ttgcgaatat atgaaaatgg tgggctttga
     1561 atttcaaaat atcgaaggca acagaaagat ttccatgaag tataataact atcaaatagg
     1621 cttaaatcaa atgtatcaat cctttgggta ttatatcaat gaccataaga ataattacga
     1681 tgcggtaaat tattatttaa gacatggatt aaatctattt gaatagcaga atatatttac
     1741 tgtatttctt taaaatggta atattggtat atagtgtact ttattataat tttaaggagg
     1801 aataaaaatg aaaggtttaa gaaaaagtaa agcggttttt gcaatggctc tggtactggc
     1861 tttattgatt acttgtgggg catttcctgt agcggctcag gaaggtggtt taattattgg
     1921 tgatgcaaat ggggacggac ttgtcaatgt gactgacgcg gcaattctga atctatacgt
     1981 atccggctac acaaatacat ttccatatga tatggcggac tacacaatgg atgttaatga
     2041 tgacggatat attaacagac aggatttcga tctgctgaac aaatttatat caggttcact
     2101 ggcatcactg ccatcagcgg atatactaag gaacatgcca caaacagtat ttcaatatag
     2161 ttatgtaaat ggtgcatggg gatatagaaa tataagatta agtatagata aacaaggaaa
     2221 tatttacacc agcggaaaca taacaccaaa tgtagatccc tcaactataa aagttcctaa
     2281 aattgagatt gagtctatgt atttagattt atatctggcc tcacagggaa caataacgaa
     2341 tcctcagaat gctgcttgtg atatggggat aaatgtttat gggggaaata tcgtaaagaa
     2401 tggagctttg cagtacgtat ttttgaagca atatggcgat gttgaccagg aaaatatgtc
     2461 accctacact cagactttgg tggattggct tgacggatat gtagatatga tatactaa
//
