LOCUS       CP000033               12011 bp    DNA     linear   BCT 30-JAN-2014
DEFINITION  Lactobacillus acidophilus NCFM, complete genome.
ACCESSION   CP000033
VERSION     CP000033.3
DBLINK      BioProject: PRJNA82
            BioSample: SAMN02603047
KEYWORDS    .
SOURCE      Lactobacillus acidophilus NCFM
  ORGANISM  Lactobacillus acidophilus NCFM
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
            Lactobacillus.
REFERENCE   1  (bases 1 to 12011)
  AUTHORS   Altermann,E., Russell,W.M., Azcarate-Peril,M.A., Barrangou,R.,
            Buck,B.L., McAuliffe,O., Souther,N., Dobson,A., Duong,T.,
            Callanan,M., Lick,S., Hamrick,A., Cano,R. and Klaenhammer,T.R.
  TITLE     Complete genome sequence of the probiotic lactic acid bacterium
            Lactobacillus acidophilus NCFM
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 102 (11), 3906-3912 (2005)
   PUBMED   15671160
REFERENCE   2  (bases 1 to 12011)
  AUTHORS   Altermann,E., Russell,W.M., Azcarate-Peril,M.A., Barrangou,R.,
            Buck,B.L., McAuliffe,O., Souther,N., Dobson,A., Duong,T.,
            Callanan,M., Lick,S., Hamrick,A., Cano,R. and Klaenhammer,T.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2004) Department of Food Science, North Carolina
            State University, Raleigh, NC 27695-7624, USA
REFERENCE   3  (bases 1 to 12011)
  AUTHORS   Altermann,E., Russell,W.M., Azcarate-Peril,M.A., Barrangou,R.,
            Buck,B.L., McAuliffe,O., Souther,N., Dobson,A., Duong,T.,
            Callanan,M., Lick,S., Hamrick,A., Cano,R. and Klaenhammer,T.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2007) Department of Food Science, North Carolina
            State University, Raleigh, NC 27695-7624, USA
  REMARK    Sequence update by submitter
REFERENCE   4  (bases 1 to 12011)
  AUTHORS   Altermann,E., Russell,W.M., Azcarate-Peril,M.A., Barrangou,R.,
            Buck,B.L., McAuliffe,O., Souther,N., Dobson,A., Duong,T.,
            Callanan,M., Lick,S., Hamrick,A., Cano,R. and Klaenhammer,T.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2007) Department of Food Science, North Carolina
            State University, Raleigh, NC 27695-7624, USA
  REMARK    Sequence update by submitter
COMMENT     On Nov 1, 2007 this sequence version replaced CP000033.2.
FEATURES             Location/Qualifiers
     source          1..12011
                     /organism="Lactobacillus acidophilus NCFM"
                     /mol_type="genomic DNA"
                     /strain="NCFM"
                     /db_xref="taxon:272621"
     gene            complement(1..1434)
                     /gene="epsI"
                     /locus_tag="LBA1724"
     CDS             complement(1..1434)
                     /gene="epsI"
                     /locus_tag="LBA1724"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q8KUK6|2.A.66.2.16"
                     /protein_id="AAV43536.1"
                     /translation="MKVVRNYLYNVGYQVLAIIVPLITSAYVSRVLRPEGVGANAFTNS
                     IIQYFILFASMGIGYYGNRQIAYVREDSHKMAQTFWEIQIVKTMMTLLSIIAFEIFMMF
                     YTRQPEYMIAQSINLVAVAFDISWFYEGIEDFKVTVLKNSFVKLISMAAIFLLIKGPYD
                     VTLYIVVLAVSTLLGNLTLWPNIKKDLPKININILNPWRHFLPMAELFIPQIATQVYVQ
                     LNKTMLGGMVSETSAGYYQYSDNLVKLILALVTATGTVMLPHVANAVSKGNMREVNQML
                     YKSFDFVSAVSYPMMFGLAAISLTLAPKYYGPGYDPVGPAMMIESIVILMIAWSNVLGV
                     QYLLPINHQRDFTVSVTVGAVVNVILNIPLIHNWGLDGAMWSTVISEISVTLYQLWAVK
                     GLLNYRQLFLNSWKYLLSGIIMFVLVFWMNQHFQDSWLMMGLEIIIGVIIYGVMIIFFK
                     APIIDQAKKIVNDKLKNVK"
     gene            complement(1440..1966)
                     /gene="epsJ"
                     /locus_tag="LBA1725"
     CDS             complement(1440..1952)
                     /gene="epsJ"
                     /locus_tag="LBA1725"
                     /EC_number="5.4.99.9"
                     /note="COG0562 UDP-galactopyranose mutase"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="AAV43537.1"
                     /translation="MLGDPDITVKLNTDFFDNKDDYLKNFDKIIYTGMIDKFFDYKLGE
                     LEYRSLRFETEEKDVNNYQGNAVINYTEAEIPYTRVIEHKHFEFGKGDNDKTIITREYP
                     ADWKRGDEPYYPINNKRNNDLYAKYAELAKKQDKVIFGGRLGQYKYYNMDQVIAAALDT
                     VNSEFGD"
     regulatory      complement(1961..1966)
                     /regulatory_class="ribosome_binding_site"
                     /gene="epsJ"
                     /locus_tag="LBA1725"
     gene            complement(2151..2552)
                     /locus_tag="LBA1726"
     CDS             complement(2151..2552)
                     /locus_tag="LBA1726"
                     /EC_number="5.4.99.9"
                     /note="potential frameshift to 1725; UDP-galactopyranose
                     mutase"
                     /codon_start=1
                     /transl_table=11
                     /product="STP|Pyr_redox"
                     /protein_id="AAV43538.1"
                     /translation="MKTYLVVGSGLFAAVFAHEAAKRGNKVKVIEKRDHIAGNIYTKEI
                     DGIQVHKYGAHIFHTSNKKVWDYVHQFADFNRYTNSPVANYKGHMYNLPFNMNTFSEMW
                     GVRTPQEAMAKINEQRQEMAGKEPQNLEE"
     gene            complement(2549..3844)
                     /locus_tag="LBA1727"
     CDS             complement(2549..3844)
                     /locus_tag="LBA1727"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="AAV43539.1"
                     /translation="MILLVITLLGLSITSYYLNNRNLVSPAFLLSTTFFICSLVALINQ
                     NKWQLILNRKTYLVICGAILEFIIVTYLVNKLLSVVKFQYKNSKKSKLNAPYISTRKSY
                     ILFAIQLLLIIYVIRNLKEVTKINNIFQAASALNQSSLPNYIGQPIALSKIANIFLAFI
                     LASGLYTGYVFFLYIIVKKNFRFDLFINMFISILAPFVTGSRGNSIYMIISWVIYCYLI
                     LWKNNKLNFKMQFKFVMRITLVLIILLLLLPLTAVLFGRRMDNWDEYLSIYIGAQIKNL
                     NEFILNNNFPLQTSIFGQQTFFTIIPLVSKLIGLNIPSYKLDLPYQAIGSLSLGNVYTT
                     FYPWLYDFGYKGVFLLTLIMAIVVEFIYHLALHSKLQFGLSILLYGYLGSFVALLFFSN
                     KFYEGLNSTLILIIMSWILLIYIFKQKKGKIK"
     gene            complement(3869..4909)
                     /locus_tag="LBA1728"
     CDS             complement(3869..4909)
                     /locus_tag="LBA1728"
                     /codon_start=1
                     /transl_table=11
                     /product="GT2_Glycos_transf_2|GT2"
                     /protein_id="AAV43540.1"
                     /translation="MKKKTLTIGVAAYNAAKIISNCLDSLLIDEVKNDIEVLVINDGST
                     DNIQSVVATYENRYPNSIRLINKKNGGHGSTINKTIEEAKGEYLKMVDADDSVEKQGFI
                     ALVRHLKKTNADVVMSPYYRVNIVNNHKKLIGYLKSSNKESIVDNKLVNLQDIYQDLLV
                     AMHSLTYRTTLLKENNYKIDEHCFYVDVEYSIYYFLKAKNVLLLNEPVYNYNIGSSDQS
                     VNIDNMRARRAQHLRVAKSMVNFYKKERNKIPSYMKSFFKNNIVNMILVNEYKLLMSLK
                     SSKNSKIELIKFDKYLKKNSIELYEGVTTFNNSKKIKLLKILRKINFNGYTIVHNLCKK
                     SLTTHI"
     gene            complement(4906..5562)
                     /locus_tag="LBA1729"
     CDS             complement(4906..5562)
                     /locus_tag="LBA1729"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AAV43541.1"
                     /translation="MKKLLKFYPNKPYKINFENKDDDTSRISDIIETMRSDQRLKILYQ
                     GVFDKDRKLDEFAKTIENESDKFCLYIMGKQNKYSKELCKKYPHIRYIPYITSPNHLKI
                     TREADIGLLPYFPVRVGNNSILNALYCAPNKIYEYSAFGKPMLGTDVLGLKYPFEKYKM
                     GYTVSKLEEKEILEKLNKILVNYQKMSENSRRFFQSVNWDKKIDEILEIKEEEKD"
     gene            complement(5531..6064)
                     /locus_tag="LBA1730"
     CDS             complement(5531..6052)
                     /locus_tag="LBA1730"
                     /note="COG0058 Glucan phosphorylase"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AAV43542.1"
                     /translation="MRVLIVHNREISDFPPVRSLVDALIANKVKTTIITRDENNVLDSY
                     GDKLKVLELLPYASNGNFKNTYKFFENRKLIRKWVKKEMKNNDIIWTTTDATVREIGPD
                     LLNYKHVMQLMELVEYVPKFPLIPKKNLFKFNITKYAHHAWKIVVPEINRAYIQKVWWD
                     LEKTPKILPK"
     regulatory      complement(6057..6064)
                     /regulatory_class="ribosome_binding_site"
                     /locus_tag="LBA1730"
     gene            complement(6065..6960)
                     /locus_tag="LBA1731"
     CDS             complement(6065..6946)
                     /locus_tag="LBA1731"
                     /note="COG1216 Predicted glycosyltransferases"
                     /codon_start=1
                     /transl_table=11
                     /product="GT2_Glycos_transf_2|GT2"
                     /protein_id="AAV43543.1"
                     /translation="MNNIAAIVVTYNRKKLLKKCIKGLKEQTEKADIIIIDNMSTDGTL
                     EMLQPLINSNEIIYHSTGKNIGGAGGFYEGIKLAYEMGYKYFWLMDDDCIPTSSALEKL
                     MNVVNEKKNFGFLVSKALWKDGEVCKMNIPKIKLTKQVNDFSKKVIQIKMGTFVSFLTS
                     REVVEKVGLPIKEFFIWGDDLEYSQRISQKFPSYLVTNSLVYHETKLNEGSNIALDSVD
                     RLKRYKLAYRNEVVLFKEMGLQGQMYQYFRLVLHCGRILFKSKDHKKERLNIIFQGTRA
                     GYNFHPVIKNIK"
     regulatory      complement(6955..6960)
                     /regulatory_class="ribosome_binding_site"
                     /locus_tag="LBA1731"
     gene            complement(6963..7748)
                     /gene="epsF"
                     /locus_tag="LBA1732"
     CDS             complement(6963..7736)
                     /gene="epsF"
                     /locus_tag="LBA1732"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyl transferase"
                     /protein_id="AAV43544.1"
                     /translation="MKIKILVAAHKKFPMPNTDGYMPVLVGAAKNYKPDIKYQRDDEGD
                     NISFKNPNYNELTAVYWAWKNLENVDAVGLVHYRRLFFDTKPYTLSNVISIKKIEELLT
                     QYDVILPKKRNYYIETNYSHYIHAHHREPLDRTRDVVAQNYPQYLSNFDKAMHRRKAHM
                     FNMFIMRKKVFESYCNFVFGVLSKLENIIDISNYSVQEARVYGYISELLMDVWLETNGI
                     KYVEMPWGQIGGKNNVKKAIFLIKRKMGIKTKTHF"
     regulatory      complement(7741..7748)
                     /regulatory_class="ribosome_binding_site"
                     /gene="epsF"
                     /locus_tag="LBA1732"
     gene            complement(7745..8413)
                     /gene="epsE"
                     /locus_tag="LBA1733"
     CDS             complement(7745..8398)
                     /gene="epsE"
                     /locus_tag="LBA1733"
                     /note="EpsE"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|H8E4X1|9.B.18.1.2"
                     /protein_id="AAV43545.1"
                     /translation="MMAMERLKVNPKKVYNRPFYHTVKRLFDIVASAIGLVLLSPLFLY
                     LIIRIRHEDGGPAFYSQERIGKNEKPFRMWKFRSMVVNADKMLDKLEDQNEVNGAMFKI
                     KDDPRITKIGHMIRKYSLDELPQLYNVLIGDMSLVGPRPPLPSEVEEYTDYDKQRLLVM
                     PGCTGLWQVTRRNEADFDEMVWLDIVYINHSGLWEDFKLIIKTVLVMIHPNGAY"
     regulatory      complement(8404..8413)
                     /regulatory_class="ribosome_binding_site"
                     /gene="epsE"
                     /locus_tag="LBA1733"
     gene            complement(8488..9258)
                     /gene="epsD"
                     /locus_tag="LBA1734"
     CDS             complement(8488..9258)
                     /gene="epsD"
                     /locus_tag="LBA1734"
                     /note="EpsD"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="AAV43546.1"
                     /translation="MTLVDIHCHILPGIDDGSKDWETSIKLARDAVKDGITHAVCTPHT
                     LNGKYLNHKDDVIRLTENFQDMLDEAKIPLTVFPGQEVRISGDLPDALDNDDILFLDEE
                     GQYMLLEFPSDDVPTYAKDMIFSIQQRGITPIVVHPERNSRILKEPHILQELIEQGCLV
                     QITASSYVGTFGQKIEEMSRRFIEAGQCACFASDAHDLPKRQYEYSAALKKLSNEFGSG
                     RAQEFEDNARAIVNGDNVQLNWRPLKKKKKFWLF"
     gene            complement(9261..10054)
                     /gene="epsC"
                     /locus_tag="LBA1735"
     CDS             complement(9261..10043)
                     /gene="epsC"
                     /locus_tag="LBA1735"
                     /note="EpsC"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P39851|8.A.3.2.1"
                     /protein_id="AAV43547.1"
                     /translation="MPLFKKKRGTDETIKHGAKLITVAKPKSPIAEQFRTVRTNINFMA
                     VDHDIKSLAFTSANISEGKSTVAANVAVTYAQAGRKVLLVDADLRRPTVHSTFNLSNHV
                     GLSTVISSTAKEVDLDSVVQESGVDNLYVLTAGPMPPNPAELIGSKRMRDFVKLTEEHY
                     DLVIIDLAPVLEVSDTQELASHLDGVVLVVRQGKTQKMAIKRAVEMLEFAKARILGYIM
                     NDVSSDNAGYGYGYGYGYGYGYGEEDTKKKGLFSKFRK"
     regulatory      complement(10047..10054)
                     /regulatory_class="ribosome_binding_site"
                     /gene="epsC"
                     /locus_tag="LBA1735"
     gene            complement(10059..10945)
                     /gene="epsB"
                     /locus_tag="LBA1736"
     CDS             complement(10059..10934)
                     /gene="epsB"
                     /locus_tag="LBA1736"
                     /note="EpsB"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P72367|8.A.3.2.4"
                     /protein_id="AAV43548.1"
                     /translation="MEQKQEQENTIDLTQLLRICRKHIWALILWSVGLALVGWGVSEFV
                     ISPKYTSTAQLLVNQKSRNNDPNAAYATQQANMQMVTTYKDIVTSNKILTEASNRLANP
                     TVVVKKAQKAVYRTDENGRRRLVRKAQPAVIERSGKSYSVSASELAKSISVGTQQQSQV
                     FSISAEADTPAKAKAEVNAVAETFRKEIPTIMSVNNVTIVANGTNGVQSSPNVKLFTLA
                     GFVVGLVLSFAVVIIREMSNTTVRDDEFLTRELGLTNLGQIAHFHLSSSFTIKKSANMT
                     NRGQAKRRRV"
     regulatory      complement(10940..10945)
                     /regulatory_class="ribosome_binding_site"
                     /gene="epsB"
                     /locus_tag="LBA1736"
     gene            complement(10956..>12011)
                     /gene="epsA"
                     /locus_tag="LBA1737"
     CDS             complement(10956..12011)
                     /gene="epsA"
                     /locus_tag="LBA1737"
                     /note="EpsA"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="AAV43549.1"
                     /translation="MAENNQPNNDVRRHHHHRHHRHHHRKFWHWFWIVIGVIVVIVLFV
                     CGMVYKNLRDTTQNMYTPVAKTTKSNKGRNLDNLLAQKKPINILLLGTDTGAMGRSWKG
                     RTDTIMMMAINPKTNSTSIVSIPRDSNAIFPDFPQYGVTKINSAYTLGGVGETVKTLDK
                     YYSVPIDGYIMINMGGLKKAIDQVGGIDVTSPLTFDNMGYHFQEGKTYHMDGKKALAFA
                     QLRHGDPRQDYGRQDRDRRVVMALLKKSISPTTLLNTKFLNSISSEMQTDLTMNQMYKI
                     GMDYRHATDNLSQDHAQGVSKQTQNPKFGTMEIEVVSRQERQRVSDKLRAALGLPKVRV
                     AANSASYVMNQ"
ORIGIN
        1 ttattttaca tttttcaatt tatcattaac tattttcttt gcctgatcaa taataggtgc
       61 cttaaaaaat ataatcatta caccatatat tattacacca ataataattt ctaatcccat
      121 cattagccaa ctatcttgga aatgctgatt catccaaaat acaagaacaa acataattat
      181 ccctgacaat aaatatttcc aagaatttaa aaaaagttgt ctataattta gtaatccttt
      241 aacagcccat agttgataca aagtaactga aatttcagaa ataacagtag accacattgc
      301 accatctaaa ccccaattat gaattaacgg tatatttaaa ataacattaa caaccgcacc
      361 tactgttact gaaactgtaa aatcacgttg atgattaata ggtaacaaat attgaactcc
      421 aagtacattg ctccaggcaa tcatcaatat aacaatagat tctatcatca tagctgggcc
      481 aacaggatca taccctgggc cataatattt tggtgctaat gttaaagaaa ttgcagctaa
      541 gccaaacatc attggatatg acacagccga aacaaaatca aatgatttat ataacatttg
      601 attgacttct cgcatatttc cttttgacac ggcattagca acatgaggaa gcatgacagt
      661 acctgttgca gttactagag ctaaaatcag tttaaccaga ttatcagagt attgatagta
      721 tcccgcggaa gtttcactta ccataccacc aagcatagtt ttatttaatt gaacatatac
      781 ttgtgtagca atttgtggaa taaataattc tgccataggc aaaaaatgtc gccatggatt
      841 taaaatattt atgttaattt taggaagatc ttttttaata ttaggccata aagtcaaatt
      901 tcctaataat gttgaaaccg ctaaaacaac aatatataaa gtcacatcat atggtccctt
      961 aattaatagg aaaattgctg ccatagatat taatttaaca aaagaatttt ttaagactgt
     1021 aaccttaaag tcttctattc cttcgtaaaa ccaagatata tcaaatgcaa ctgccactaa
     1081 attaatggat tgagcgatca tatattcagg ttgtcttgta tagaacatca tgaaaatttc
     1141 aaatgcaata atagacaaaa gtgtcatcat tgtttttact atttgaattt cccaaaatgt
     1201 ttgcgccatc ttatgtgaat cttcacgtac ataagcaatc tgacgattac cataatagcc
     1261 tatgcccata cttgcaaaaa gtataaaata ttgaattatt gaattagtaa atgcatttgc
     1321 accaacacct tcaggtctaa gtacccgact aacataagct gatgtaatta aaggtacaat
     1381 aattgctagt acttgatatc caacattata aaggtaattt cttacaacct tcataaaact
     1441 taatcaccaa attcactatt aacggtatca agcgctgctg caataacttg atccatattg
     1501 taatacttat attgaccaag acgtccacca aaaataactt tatcctgctt tttagctaat
     1561 tctgcatact tagcatataa gtcattatta cgcttattat ttattggata ataaggttca
     1621 tcaccacgtt tccaatcagc tgggtattct cgagtgatga ttgttttatc attgtcacct
     1681 tttccaaatt caaaatgctt gtgttcaatt acacgagtat atggaatttc tgcttcagta
     1741 taattgatca ctgcatttcc ttggtaatta tttacatctt tttcttcagt ttcaaaacga
     1801 agtgaacggt attctagttc acctaattta taatcaaaga atttatcaat cataccagta
     1861 taaataatct tgtcaaagtt ctttaagtaa tcatctttat tatcaaaaaa atcagtattt
     1921 aatttaactg tgatatcagg atcacccaac atattttcaa ccatcttagt ataaccgcca
     1981 attggaattc cttgataatc atcattaaag taattattat cataaactaa tcgaactgga
     2041 agacgtttaa tgataaaagc tggaagctct gtagcttttc tgccccattg tttttcagta
     2101 tatcctttga ttaacttttc atagatatct ctaccaatta aagaaatagc ctactcttct
     2161 aaattttgcg gttcttttcc agccatttct tgacgttgct cattgatctt agccatagct
     2221 tcttgtggtg ttctaacacc ccacatttca ctaaaggtat tcatattgaa aggtaagtta
     2281 tacatatgtc ccttataatt ggcaacagga ctattagtat agcgattaaa atccgcaaat
     2341 tgatgaacat aatcccaaac cttcttatta gaagtatgga aaatatgagc accatattta
     2401 tgaacttgaa tgccatctat ttctttagta tagatgttgc ctgcaatatg gtctcttttt
     2461 tcaattacct taactttatt tccacgtttt gcagcctcgt gagcaaacac agctgcaaaa
     2521 agaccagaac ctacaactaa atatgtcttc actttatttt ccctttcttt tgcttaaaaa
     2581 tgtaaattag tagtatccat gacattatta ttaaaatcaa agtagaattt agtccttcat
     2641 aaaacttatt agagaaaaac aataatgcta caaagcttcc taaatatcca tataacagta
     2701 tacttaatcc gaattgcaat ttactatgta atgctaaatg atatataaat tctacgacta
     2761 ttgccatgat taatgttaaa agaaaaacac ctttataacc aaaatcatat aaccaagggt
     2821 aaaatgtcgt atacacattt cctaaagata aacttccgat tgcttgatat ggtaaatcca
     2881 acttataact aggaatattt agtccaatta attttgaaac caaaggaatt attgtaaaaa
     2941 atgtttgctg tccaaatata cttgtttgca atggaaaatt attatttaag atgaattcat
     3001 ttaaattctt tatttgtgca cctatataaa tactaagata ttcatcccaa ttatccattc
     3061 ttctaccaaa taaaacggct gttaggggca ataacaaaag taatataatc aaaactaggg
     3121 ttattctcat aacaaattta aactgcattt taaaattcaa tttattgttt ttccacaata
     3181 tcaagtaaca atatataacc caagaaatga tcatataaat agaattgcca cgcgatccag
     3241 taacaaatgg agctaatata ctaataaaca tattaataaa taagtcaaat ctaaagtttt
     3301 tctttacaat aatatataga aaaaaaacat aacctgtata taaaccactt gctaatatga
     3361 atgccaagaa aatattagca attttcgata atgcaatagg ctgccctata taattgggca
     3421 aactagattg gtttaatgcc gaagcagctt gaaaaatatt atttatttta gtaacttctt
     3481 ttagattcct aattacataa ataattaata ataactgtat agcaaacaat atgtatgatt
     3541 ttctcgtgct aatataaggc gcatttaatt ttgatttttt actattttta tattgaaatt
     3601 taactacaga caacagttta ttaactaaat atgtcactat aataaattct aatatggcac
     3661 cacaaataac taagtatgtt tttctattta aaataagttg ccatttattt tgattaatta
     3721 aagcaaccaa agaacaaata aaaaaagttg tgcttaaaag aaatgctgga gaaactaaat
     3781 ttctattatt taaataataa gatgttatag ataaccctaa gagagtaatg actaataaaa
     3841 tcataaatat taaaccttac taattaattt atatatgagt agtcaacgac tttttacata
     3901 aattatgaac tatggtatat ccattaaaat ttatttttct caatatttta agtagtttta
     3961 tttttttact attattaaag gttgtgacac cctcatataa ctcaatagaa ttctttttta
     4021 aatatttatc gaattttatc aattctattt ttgagttttt acttgatttt agtgacatta
     4081 ataacttata ttcattaact aagatcatat taactatatt atttttaaaa aagcttttca
     4141 tatatgaagg aattttattt ctttcttttt tataaaagtt caccatactt tttgctaccc
     4201 ttaagtgttg cgcccttcta gctctcatat tatctatatt aacactttga tcagaggaac
     4261 ctatattata gttatataca ggttcattaa gtaataatac attcttagct tttaagaaat
     4321 agtaaataga atattcaaca tccacataaa aacaatgttc atctatttta taattatttt
     4381 cctttaacaa agtagtcctg taagtcaaac tatgcattgc taccaataag tcttgataaa
     4441 tatcctgcag atttacgagt ttattatcaa caatactctc cttattcgaa gactttaaat
     4501 aacctattaa ttttttatga ttattaacaa tatttactct ataatacggt gacataacaa
     4561 catcagcatt ggttttcttc agatgacgta ctaatgctat gaaaccttgt ttttcaacac
     4621 tatcatcagc atccaccatt tttaaatact cgcccttagc ttcttctatt gtcttattaa
     4681 ttgtagaacc atgtcctcca ttttttttat taattaaccg tatactatta ggatatctat
     4741 tttcatacgt agctacaaca ctttgtatgt tatctgtgga gccatcatta ataactaaaa
     4801 cttctatatc attttttact tcatcaatga gtaaactatc taaacaatta gaaataattt
     4861 ttgccgcatt atatgcagct accccgatag ttaatgtttt tttcttcaat ctttttcctc
     4921 ttctttaatc tccaaaattt catcaatttt cttatcccaa ttaacagatt gaaagaaacg
     4981 tctggaattt tcactcatct tctgataatt gacaagtatt ttatttaatt tttctaaaat
     5041 ttctttttct tctaattttg aaaccgtata ccccatttta tacttttcaa aaggatattt
     5101 taaccctaag acatcagttc ctagcattgg cttaccaaat gcagagtatt cataaatttt
     5161 attcggtgca caatataacg catttaatat tgaattatta ccaactctta ctggaaaata
     5221 tggtagtaat ccaatatctg cttctcttgt tattttcaaa tgattcggtg atgttatata
     5281 tggtatatat cgaatatggg gatatttttt acaaagttct ttactatatt tattttgttt
     5341 tcccattata taaagacaaa atttatcact ttcattttca attgtctttg caaattcatc
     5401 taattttcta tctttatcaa atacaccttg atataatatt tttaatcgtt gatcacttcg
     5461 cattgtctct ataatatctg atattcttga agtatcatca tctttatttt caaagttaat
     5521 tttatatggt ttatttgggt aaaattttag gagttttttc aagatcccac catacctttt
     5581 gaatataagc tctatttatt tcaggtacca ctatcttcca agcatgatgg gcatatttag
     5641 ttatattgaa tttaaataaa ttttttttag gaattaaagg gaattttggc acgtattcaa
     5701 ctaattccat taattgcata acatgtttgt aattaagcaa atcaggtcca atttctcgaa
     5761 cagttgcatc cgtagttgtc caaataatat cattattttt catctccttc tttacccatt
     5821 ttctaattag ctttctattt tcaaaaaatt tataagtatt tttaaaatta ccatttgaag
     5881 catatggcaa taattccaaa acttttaatt tatcaccata actatctaat acattatttt
     5941 catctcgagt aattatagtg gttttaactt tattagctat taaagcatca accagacttc
     6001 taacaggcgg aaaatcgcta atttctctat tatgtacaat taaaactctc atatttttcc
     6061 ttttctattt gatatttttt attacgggat ggaaattata tccagctcgt gtgccttgaa
     6121 aaataatatt caatcgttct tttttatgat ctttgctctt aaacagaatc cgtccacaat
     6181 gcagaactaa acgaaaatat tgatacattt gcccttgcaa tcccatttct ttaaacaaaa
     6241 caacttcatt tctatatgca agtttatatc gttttaaacg atcaacgcta tctaatgcaa
     6301 tattagaccc ttcattcaat ttagtttcat gatacacaag actatttgta accaaataag
     6361 atggaaattt ttgggaaatt ctttggctat attccaaatc atccccccaa ataaagaatt
     6421 cttttatagg taatcctact ttttctacta cttctcttga ggttagaaac gaaacgaatg
     6481 ttcccatttt aatttgaata accttttttg agaaatcatt tacttgcttg gtaagtttta
     6541 tcttaggtat attcatttta catacttctc catctttcca tagggcttta cttaccaaaa
     6601 aaccaaaatt ttttttctca tttactacat tcattaattt ttctaaagcg gaagaagttg
     6661 gaatacaatc atcatccatt aaccaaaaat atttatatcc catttcataa gctagtttta
     6721 ttccctcata aaagccacca gcaccaccaa tattttttcc agtcgagtga taaattattt
     6781 cgtttgaatt aattaaaggc tgtaacattt ctaaagtacc atcagtactc atattatcaa
     6841 taataatgat atcagctttt tctgtttgct cttttagtcc tttaatacat ttttttaata
     6901 atttttttct attatatgta actactatcg ctgctatatt attcataatt tttccctaca
     6961 atttaaaagt gtgttttagt ctttattccc atctttcttt ttataagaaa aatagccttt
     7021 ttcacattat tcttaccgcc aatctgtccc catggcattt caacatattt gattccatta
     7081 gtttcaagcc aaacatccat taacaattct gaaatatatc catatactct tgcttcttgt
     7141 acagaataat ttgaaatatc tattatattt tctaatttac tcaatacacc aaaaacaaaa
     7201 ttacaatatg attcaaaaac ctttttacgc attatgaaca tattaaacat atgcgctttt
     7261 cttctatgca ttgctttatc aaaatttgat aaatattgtg gataattttg ggcaactaca
     7321 tctctcgttc tatctaaagg ttcccgatga tgagcatgta tataatgaga ataatttgtc
     7381 tcaatataat agtttctttt tttaggaagg attacatcat attgagttag taattcttct
     7441 atttttttga tactaataac attacttaac gtatacggtt tcgtatcaaa aaagagacga
     7501 cgatagtgga caagacctac tgcatctaca ttttccaaat tcttccaagc ccaataaaca
     7561 gcggtcagtt cattataatt tggattctta aaagatatat tatcaccctc gtcgtctctt
     7621 tgatatttaa tgtctggttt ataattttta gctgcaccta ctaacacagg catatatcca
     7681 tcagtatttg gcattggaaa tttcttatgt gcagcaacta aaatttttat tttcataaaa
     7741 ctccttaata agccccattc ggatgaatca taactaatac ggtcttaata atcaatttaa
     7801 agtcttccca caatccagaa tgattgatat aaacaatatc aagccatacc atttcatcaa
     7861 agtctgcttc atttctacgc gttacttgcc aaagtccagt acacccaggc ataactaata
     7921 gacgttgttt atcataatcc gtatactctt caacttcaga aggaagaggt ggacgtggtc
     7981 ctactaacga catgtctccg atcagaacat tgtataattg tggtaattca tcaagactat
     8041 atttacgaat catatgccca attttggtaa ttcttggatc atctttaatc ttaaacatag
     8101 ctccattgac ttcattttga tcttctaatt tatccagcat cttatctgca ttaacaacca
     8161 tggaacgaaa cttccacatt ctaaatggct tttcattctt accaattcgc tcttgcgaat
     8221 aaaatgctgg gccaccatct tcgtgtctaa ttctaataat tagatacaaa aataatggtg
     8281 atagaagaac taatccaatc gcactggcta cgatatcaaa taaccgctta actgtgtgat
     8341 aaaaaggtcg attatatacc ttcttaggat taactttcag tctttccatc gccatcatta
     8401 ttcctcttat cctctttttt acttagtaac ttacttaaaa accaactact cctaaagctg
     8461 tattataata cttctttatt tctatgatta aaataaccaa aacttcttct ttttcttaag
     8521 tggtcgccaa ttcaattgaa cattatcgcc gttcacaatt gcgcgagcat tatcttcgaa
     8581 ttcttgagca cgtccacttc caaattcatt gctcaacttc tttaatgctg cactatattc
     8641 atattgtctc ttaggcagat catgagcatc ggatgcaaag caagcacatt gtccagcttc
     8701 aatgaagcgt ctactcattt cttctatttt ctggccaaat gttccaacat atgaactagc
     8761 agtaatttga actagacaac cttgctcaat taattcttgc aagatatgtg gctcttttaa
     8821 gattctactg ttacgttccg gatgcacaac aattggtgta ataccacgtt gttgaatgct
     8881 aaagatcata tctttagcat aagttggtac atcatcactt ggaaattcca ataacatgta
     8941 ttgtccctct tcatctaaga agagaatgtc atcattatct aatgcatcag gcaaatcgcc
     9001 tgaaatacgc acttcttgcc caggaaatac tgttaatgga atctttgctt catcaagcat
     9061 atcttgaaag ttctcagtta atcgaatcac atcatctttg tgattaagat attttccatt
     9121 taacgtatgt ggtgtacata ctgcatgtgt tataccatct tttacagcat cccttgccaa
     9181 cttaattgat gtttcccaat cttttgatcc atcatcaatt cctggcaaaa tgtgacagtg
     9241 aatatcaact aaagtcataa ttacttccta aatttagaga acaatccttt tttctttgta
     9301 tcttcttctc cgtagccata accatagccg tagccgtaac catagccata acccgcatta
     9361 tcagaactta catcattcat gatgtaaccc aagatacgtg cctttgcaaa ttcaagcatt
     9421 tcaacagcac gcttaatggc catcttttgc gtcttccctt ggcgaactac taagacaacc
     9481 ccatctaagt gactagcaag ttcttgtgta tcagatactt caagaacagg agctaagtcg
     9541 ataataacta aatcgtagtg ttcttcagta agtttaacaa agtcacgcat acgcttagaa
     9601 cctatcagtt ctgccgggtt aggtggcatc ggaccagccg ttaacacgta aagattatct
     9661 acgccgctct cttgtactac gctatcaaga tcaacttcct tagcagttga agagataacc
     9721 gtactaagac caacatgatt acttaagtta aaggttgagt gcactgtagg tctacgtaag
     9781 tcggcatcga ccaataagac tttacgacct gcttgagcat aagtaacagc aacgttagca
     9841 gccacagtag atttaccttc actaatatta gcagaagtaa atgctaaaga cttaatgtca
     9901 tgatccactg ccataaagtt aatattagta cgaacagtac ggaattgttc agctattgga
     9961 ctctttggct tagctacagt gattaactta gcaccatgtt taatagtttc atctgtacca
    10021 cgctttttct taaataatgg cattttgtcc tcctactctt atactctacg tctcttagct
    10081 tgtccacgat tagtcatatt tgcactcttt ttaatagtaa atgaagatga caagtggaag
    10141 tgagcaattt gacctaagtt agttaagcct aattcacgag ttaagaattc atcgtcacga
    10201 acagtagtat tactcatttc acgaataata actacagcaa agctcaatac taacccaact
    10261 acaaatccag ctagagtgaa caacttaacg ttaggtgagg attgaacacc attagtacca
    10321 tttgccacaa tcgttacgtt attaacgctc ataatagtag gaatttcttt acggaacgtt
    10381 tcagctactg catttacttc tgccttcgcc ttagctggtg tatcggcttc cgctgaaatt
    10441 gagaagactt gtgattgttg ttgggtacct actgagatac tcttagcaag ttcacttgca
    10501 gaaactgaat aactcttacc gcttcgttca attacagctg gttgagcttt tcttactaat
    10561 cttcttctac cattttcatc agttctgtac actgcttttt gtgctttttt cacaacaaca
    10621 gttggattgg ccaaacgatt agaagcttct gttaagatct tgttacttgt tacaatgtct
    10681 ttataagtag taaccatctg catgttagct tgttgagtcg cataagctgc gtttggatca
    10741 ttgttacgac tcttttggtt aactaataat tgagcagttg aagtatattt aggagaaatt
    10801 acaaattctg aaactcccca gcccactaag gcaagaccta cactccaaag aatcaatgcc
    10861 cagatatgct tacggcaaat gcgtaataat tgggtaagat cgattgtatt ttcttgttct
    10921 tgtttttgtt ccatatctct ttccttatac tattactatt gattcatgac atagctggca
    10981 ctattagcag caactcgtac cttaggaaga cctaatgctg ctcttaattt atcagacact
    11041 ctttgccttt cttgtctact tactacttcg atttccatag taccaaactt aggattctga
    11101 gtttgcttac ttacaccttg agcatgatct tgtgacaagt tatctgttgc atgtctataa
    11161 tccatcccga tcttgtacat ttgattcata gtcaagtcag tttgcatttc acttgagatt
    11221 gaattcaaga atttagtatt aagtaatgta gtaggtgaga tagacttctt aagaagtgcc
    11281 atgacaacac ggcgatcacg gtcttgacga ccataatctt gacgtggatc cccatgtcta
    11341 agttgagcaa aggctaatgc cttcttacca tccatatggt aggtcttacc ttcttggaag
    11401 tggtatccca tattgtcgaa agtcaatggt gaagttacat caataccacc aacttgatca
    11461 atagccttct taagaccacc catgttgatc ataatgtaac cgtcaattgg cacactatag
    11521 tatttatcca atgtcttaac tgtttcacca actccgccta gtgtataagc agagttaatc
    11581 ttcgttactc catattgtgg gaaatctggg aagattgcat ttgaatcacg tggaatagat
    11641 acaattgacg tactattagt cttaggatta attgccatca tcatgatggt atcagtacgt
    11701 cctttccagc tacgtcccat tgctccagta tcagtaccga gcaaaagaat attgattggt
    11761 tttttctgcg ctagcaaatt atcaagatta cgtcccttgt tgctcttagt tgtcttagca
    11821 acgggagtat acatattttg cgtggtatcg cgtaaattct tataaaccat accacaaaca
    11881 aaaagtacaa tgactacaat aacgccgatc acaatccaga accaatgcca aaacttacga
    11941 tggtgatgac ggtgatgtct atggtggtga tggcgacgta catcattatt tggttgatta
    12001 ttttctgcca t
//
