LOCUS       KX156850               13436 bp    DNA     linear   BCT 24-MAY-2016
DEFINITION  Franconibacter pulveris strain G3872 O-antigen gene cluster, partial
            sequence.
ACCESSION   KX156850
VERSION     KX156850.1
KEYWORDS    .
SOURCE      Franconibacter pulveris
  ORGANISM  Franconibacter pulveris
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Franconibacter.
REFERENCE   1  (bases 1 to 13436)
  AUTHORS   Wang,M., Arbatsky,N.P., Xu,L., Shashkov,A.S., Wang,L. and
            Knirel,Y.A.
  TITLE     O antigen of Franconibacter pulveris G3872 (O1) is a
            4-deoxy-D-arabino-hexose-containing polysaccharide synthesized by
            the ABC transporter-dependent pathway
  JOURNAL   Microbiology (Reading, Engl.) (2016) In press
   PUBMED   27166227
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13436)
  AUTHORS   Wang,M., Arbatsky,N.P., Xu,L., Shashkov,A.S., Wang,L. and
            Knirel,Y.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2016) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, #23 Hongda Street, TEDA, Tianjin
            300457, China
FEATURES             Location/Qualifiers
     source          1..13436
                     /organism="Franconibacter pulveris"
                     /mol_type="genomic DNA"
                     /strain="G3872"
                     /isolation_source="tea"
                     /db_xref="taxon:435910"
                     /collection_date="24-Jul-2008"
                     /note="serogroup: O1"
     misc_feature    <1..>13436
                     /note="O-antigen gene cluster"
     gene            118..1182
                     /gene="rmlB"
     CDS             118..1182
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="ANF28848.1"
                     /translation="MKILVTGGAGFIGSAVVRNIINNTKDEVLVVDSLTYAGNLESLAP
                     VAQNSRYRFSHTDITNSVKIEELFNEYAPDIVMHLAAESHVDRSIDGPAAFIHTNVIGT
                     FVLLDAARNYYSKLNAEKKASFRFHHISTDEVYGDLHGTDDLFTEDTPYAPSSPYSASK
                     ASSDHLVRAWNRTYGLPTVITNCSNNYGPYHFPEKLIPLIILNALAGKPLPVYGDGSQI
                     RDWLYVEDHARALYKVATEGKIGETYNIGGHNERKNIDVVKTICSILDRLIVNKPKDIA
                     SFAELITFVTDRPGHDMRYAIDATKIKNDLGWVPEETFETGIEKTVHWYLDNKEWWQRV
                     LDGSYAGERLGLTN"
     gene            1193..2065
                     /gene="rmlA"
     CDS             1193..2065
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="ANF28849.1"
                     /translation="MKGIVLAGGSGTRLYPITQGVSKQLLPVYDKPMIYYPISVLMLAG
                     IKDILIITTPEDMPAFQRLLGTGEQFGINLSWAVQPSPDGLAQAFIIGEEFINNEPCAL
                     VLGDNIYFGQSFGKKLEAAAQITSGATVFGYQVLDPERFGVVEFDANYRALSIEEKPLK
                     PKSHWAVTGLYFYDNKVVEMAKEVKPSARGELEITTLNQMYLERGELQVELLGRGFAWL
                     DTGTHDSLVEASQFIHTIEKRQGMKVACLEEIAFRKGWMNKEQVAEQIKKLSKTDYGRY
                     LQLLISEKD"
     gene            2090..2980
                     /gene="rmlD"
     CDS             2090..2980
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="ANF28850.1"
                     /translation="MKILLIGKNGQVGWELQRSLSTLGELVAVDYFDTELCGDLTNPEG
                     IACTIRSVKPDVIVNAAAHTAVDKAESERELSQLLNADSVEVIAKEAAKLGALLVHYST
                     DYVFNGAGTHFRKEDEPTGPLNVYGETKLAGEIAVATNNPRHFIFRTSWVYATRGANFA
                     KTMLRLAKEKKQLAIINDQYGAPTGAELLADCTAIAIRAAVNDVSLYGTYHLVASGETT
                     WYDYAQYVFSVARELGEPLSVEEVKGVDTTAYPTPAKRPSNSRLSNLKFQTAFATVIPD
                     WKVGVKRVVSEVLGK"
     gene            2985..3533
                     /gene="rmlC"
     CDS             2985..3533
                     /gene="rmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-keto-6-deoxyglucose-3,5-epimerase"
                     /protein_id="ANF28851.1"
                     /translation="MNVIKTEIPEVLIFEPKVFGDARGFFFESFSEKIFEEAVGERIKF
                     VQDNHSQSQKGVLRGLHYQLDPHAQGKLVRCIEGEVFDVAVDIRKKSATFGHWVGVILS
                     AENKRQLWIPEGFAHGFLTLSETAQFVYKATNYYAPQSERCIIWNDPQINIEWPERDQV
                     QLSAKDEKGLLLSGAETYL"
     CDS             3541..4461
                     /note="orf5"
                     /codon_start=1
                     /transl_table=11
                     /product="GT2_Glycos_transf_2|GT2"
                     /protein_id="ANF28852.1"
                     /translation="MDKRVAILLCTYNGSKFLRKQLDSITNQTHQSWVIFASDDGSTDD
                     TLDIIREYQQTYGGDRVVLLRGPGKGFAWNFISALESCGEGFDYYAFSDQDDEWMENKL
                     SHALSLLNDNPQKPVVYCGRTLLTNEEGDEIGFSPLFDKRPSFRNALVQSIAGGNTMVL
                     NKQARDIVIKTPKWQEIVSHDWWVYILITGCGGEIYYDPLPMIKYRQHQENIIGSNLSM
                     LARIQRIKKLMAGHFKRWNDKNIELLKPFTDKLTPENARILNSFDNNRNANIFLRIKMI
                     FEAKLYRQTIFGNIALIVAIIFKKI"
                     /old_product="rhamnosyltransferase"
     gene            4495..5325
                     /gene="wzm"
     CDS             4495..5325
                     /gene="wzm"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q9HTB8|3.A.1.103.6"
                     /protein_id="ANF28853.1"
                     /translation="MSGKLNKGLSGSLINSLWSNRSLIFQMTKRDILSKYKGSAIGVVW
                     SLINPILMLTIYTLVFSVVFKARWGNAAVDQPKTQFAVILFVGMIIHSFLAECLVRAPN
                     LILQHANYVKKVVFPLEILPVVTVLSAIFQSVISGTVLIMAFFVFNGYVHWTLIFAPLV
                     FLPLVILALGLTWLISSLGVYVRDIGQSIGVIVTVLIFLSPVFYPLSALPQWMQSIVLL
                     NPLTYIIEQSRAVIVWGQLPDFLGLAIYSVNALWISYACYFWFQKTRGGFADVI"
     gene            5315..6670
                     /gene="wzt"
     CDS             5315..6670
                     /gene="wzt"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q50864|4.D.1.3.4"
                     /protein_id="ANF28854.1"
                     /translation="MSSDLFAIEVNNVSKCYQVYDNPLKRLKEIIIPKKQQREGKVERL
                     YHDEFWALQDVSFRLKKGETVGIVGKNGSGKSTLLQIIAGTLTPTTGSVKIAGRVAALL
                     ELGAGFNNDFTGRENIYLNAVLLGLSKAEIDEKIDDIISFADIGHFIDSPVRSYSSGML
                     VRLAFAIQAQIEPEILIVDEALAVGDARFQAKCFARLKQLKERGTSILFVSHATEQIVT
                     HCDSAILINDGVMLMQDSPRVIVNKYLDLLFGKTDKKIEVKEETESSIEQHRAEGHLQD
                     ISNNLFAFEDGLSFEQRPNYNPSEYRWGDLKAEIVDYQLIQDGKHYPLVIESEHAIHLQ
                     FYIKFKDAVYRPIFGIALKTKEGVTIYNTNSEYQDIDMSRQFNAGDIMHASFSMPVNLA
                     SGDYFISVGIASCDEGGAVVPHDRRYDSIHLSVVNRVQFIGLVNLDTHISLK"
     CDS             6702..11105
                     /note="orf8"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase
                     methyltansferase"
                     /protein_id="ANF28855.1"
                     /translation="MKQLQVSFMFQLESLGYFYDNSLNVWKREDYINIGYNDGDEAEER
                     LAKIIRDAKDISLFSSELRNKCADWQTLYHLTSQRGNVLRPFAHLLKGDVLEIGAGCGA
                     ISRFLGENGGNILALEGSQRRASIAASRTRDLDNVQVVAERFDQFNPGRQFDAITLIGV
                     LEYAPMFGNGESPEKDLLIQVRKLLKPGGFLFIAIENKLGLKYFAGAPEDHIRQSMYGI
                     EGRYKSGEAKTWGYEELKQLLTETGYRYSDVLLPFPDYKLPTSIITPAGSITTGFDAST
                     LAVQSVMSDPQLPAHLTFSLQNCFPEIFKNKLGVSLANSFIFAASIDTEIPLNDSILAY
                     HYSTQRKHQYAKETVFSQNSKNEIDVAYHFFQKEYREPDTNRYVQIRKEGDKYFRGTLL
                     GQQFINVLANENWQFDDIIKLMKQWLSALEKFIKNEGDKLEFSCQVDFSLPAYFMDAIP
                     QNIIITDEGPVLFDIEWQGIDRIELGHLLFRGILLILNQAKVRPLVGQELITRKEFVQK
                     VMTSLGLVFKEDTLQGYIRKESQFQHQVTGRDINELLLWNEERPLAPVTVKHSHACLYF
                     ASNGEEFTEQNTHSFILAPGINTIRCQVNGNNQLPITKLRFDPTDSSGWFTIHSLSIKN
                     NHGEVVWQAGADSEDVDYQDILKLQEGMLNVYYSTSHDPQILLKNVPPLTELYLEAELE
                     IIQNMSLASFLEENNTHPYSPVLKKLNEISAQLEETCLKLAKTDSLNNDVEAKLRQTED
                     HLDSTTAQLNEKSTQLENIQSQLTAVTSQLTHVNALHAAAMTRINALEASTSWKVTAPL
                     RALGKVRHRTKVLASVTCRIYRQHGLRKIISRGFATLQREGWRGIVGRLRQQRMMQSFH
                     AAAPFTLNAENAVTAQGNSVSAPTVQPTTIVYNQREGYHLKQGEHEYCYVVPAQPDGLA
                     TLISDMTIKPLFSIVVPIYNTPLDLLHELIESIEAQWYPQWELILANDCSTDPQLYGAL
                     EKITNPKIKIVHLKTNSRISGATNVAIEHATGDYIVFTDHDDLLTPDCLYEMAVCINNE
                     DPDFIYSDEDKLSEDGRYVQPHFKPDWSPDTMMSTMFTCHASCVKKSLLSKTGLLRSEF
                     DGCQDWDFVLRVAEHTNKISHIAKVLYHWRIIPQSIASNLAAKEYVLQASINVRKDALK
                     RRGLAGEVESIKDYPGYFRVNYFPINKPLVSIIIPTRDNYNVLSRCIDSLLKGTKYDNY
                     EIIILDNGSTSPETLSYLEQLSKLDKIKTIRHDHPFNFSELNNIGVSNSRGDVLLFLND
                     DTEIINDDWLERMIGYAQLPHIGAVGAKLYYPNKKCVQHAGIVNLADGPGHAMLGLNEE
                     EPGYFMRNKLEYNWIAVTGACLMIERHKFEKIGRFDEDFPIAYNDVDLCFRLISSGYYN
                     VVCQAVELYHHESVSRGVDHIDVEKAQRLHMEKQRLYQKHPRYYQYDPFFNINLHPNGI
                     NFEVGC"
     CDS             11111..12928
                     /note="orf9"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="ANF28856.1"
                     /translation="MNTISGPEFNRYDAAIKYFEDMPVDALRNAKVISFDFFDTLFTRP
                     LADPEDAFDILGKQLAIDNFRKKRQLAQIEAFRHMSASGKKEINIEGIYAAFADNSRTA
                     EEMANAEYQLELKLVEPNTIIFDLFRSLKDAGKTVVVTSDMYLPARFFEQALEKFGVTG
                     VAVFSSSDENATKRDSGEIFDVIAQKLQVAPGDILHLGDNEISDVIRPREKGVKAYLYR
                     SHYLGTICQGLPLSLSIAQGMLKSHDDYHDLQAFKSLGFAYGGAASLGFLKWIKNQTVL
                     HQIDHVLFLSRDGYVMQKMVERGDVGDFPRSTYFYGSRIALTLATINDQNFVHYIPFLL
                     SGAEGLQPCEILERIGVAAPAPEVMASFGLSAETPLTAGLFSKMANFLYAWRWQILLVC
                     RRNRRGLFMYLQQLGIKSGERIALVDVGWSGTTQEAFVHAVKQFMDIDIHGYYFCLANT
                     AEKNRRKAFLQMHAMYSTENADNSIIDMIYENRVAVELFFSAPHDSIIGYEAFTGKPVG
                     DRGRGLKNDLTHSISDICAGAMLFVKSYNELCKRLSLELTPHELTMSMLSLVSNKKWQD
                     YSAITNISNFDAWGSSRLKSLCAKDYLNS"
ORIGIN
        1 tacgcgctct ggctgcgata gccaggggcg gtagcgtgca taaattatgc ccgcaagcag
       61 gaaaaataag cagtagatat ccttcaataa gccaaattac acaggtcaag caatattatg
      121 aagattctgg ttacaggtgg ggcgggattc attggttccg cagttgtacg taacattatc
      181 aataacacaa aggatgaggt tttggttgtt gatagcctta cctacgcggg caatcttgaa
      241 tcgctggcac ctgttgcgca aaattcacgc tatcgctttt cccataccga tatcactaat
      301 agcgtaaaaa tcgaagaatt gtttaacgaa tatgcaccag atattgtgat gcacctcgcg
      361 gcagagagcc atgtcgatcg ctcaattgat ggtcctgcag cttttattca taccaacgta
      421 atcggtactt ttgtgctttt ggacgctgca cgtaattatt attcaaaatt aaacgcagag
      481 aaaaaggcaa gttttcgttt tcatcacatt tctaccgatg aggtatatgg cgatttacat
      541 ggaaccgatg atttgttcac agaagatacg ccttatgcgc cgagtagccc gtattcagcc
      601 tcgaaagcat caagcgatca tctggtaagg gcatggaatc gcacctacgg cctgccgaca
      661 gtgataacaa actgttcaaa taactatggt ccatatcatt ttcccgagaa gctcattccg
      721 ctgattatat taaatgcttt ggcaggaaaa cctttaccgg tttacggtga tggttcacag
      781 attcgcgatt ggctgtatgt agaagatcat gcgcgcgcgc tatataaagt agcaaccgaa
      841 ggtaaaattg gcgaaaccta taatattggc ggtcacaacg agcgtaagaa tattgatgta
      901 gttaaaacta tatgttccat tcttgaccgt ttgattgtaa ataaacccaa agatattgcc
      961 agtttcgccg aattgatcac ttttgtaact gaccggccgg gacatgatat gcgttacgca
     1021 atagacgcaa caaaaattaa gaatgactta ggttgggttc ctgaagaaac ctttgagaca
     1081 gggattgaaa aaaccgttca ttggtatctg gataacaaag aatggtggca gcgggtatta
     1141 gacggttctt atgctggtga acgtttaggc ttaacaaact gagacaaata agatgaaagg
     1201 tattgtactt gctggcggtt ccggaacacg tttatacccc attactcaag gcgtatctaa
     1261 acaactcttg cctgtttatg ataagccaat gatttattac ccgatttctg tgctgatgct
     1321 ggcaggaatc aaagatatcc tgataataac cacaccagaa gatatgcccg cgttccagcg
     1381 cttacttggt acaggcgagc agtttggtat taatctttcc tgggcggtgc agccttctcc
     1441 agacggactg gcgcaggcat ttattattgg tgaagaattt atcaataatg agccatgcgc
     1501 actggttctc ggtgataata tttatttcgg tcaaagtttt ggaaaaaaac tggaggccgc
     1561 ggcgcaaata acttctggtg cgacggtatt tggctaccag gtacttgacc ctgaacgttt
     1621 tggtgttgtg gaattcgacg ccaactacag agcgttatct attgaagaga agccgttgaa
     1681 acccaaatcg cactgggcag taacgggtct gtatttctat gacaataaag ttgtagaaat
     1741 ggcgaaagaa gtcaaacctt ctgcgcgcgg tgagctggaa attacaacac tgaaccaaat
     1801 gtatctggag cgcggagaat tgcaggttga attacttggc cgcggtttcg catggctgga
     1861 tactggcacg cacgacagcc tggttgaagc atcgcagttt attcacacta ttgagaaacg
     1921 tcagggcatg aaggttgctt gccttgaaga aattgcgttt cgcaaaggct ggatgaataa
     1981 agagcaagta gctgagcaaa ttaaaaaatt atcaaaaact gattatggcc gttatctgca
     2041 attattgatc agtgaaaaag attaatcatg ctgcgaagaa gagaatacca tgaagatttt
     2101 attaattgga aaaaacggcc aggttggctg ggaactgcag cgctctttat caactctcgg
     2161 tgaactggtt gcagtagatt attttgacac tgaattatgt ggtgacctga ctaaccctga
     2221 aggtattgcc tgtactattc gcagcgtaaa accagatgtg attgtgaatg cagctgcaca
     2281 tactgcggtg gataaagctg aaagtgaaag agagctttct caattattaa atgccgacag
     2341 cgtagaagta atcgctaaag aggcagcaaa acttggagca ttattagtac attattcgac
     2401 tgattatgtt tttaatggtg caggtacaca ttttcgaaaa gaagatgagc ccactggccc
     2461 cttaaatgtc tatggtgaaa ccaagctagc tggcgaaatt gctgtggcaa caaataatcc
     2521 acgtcatttt attttcagaa ctagttgggt atatgcgacc aggggcgcaa actttgcgaa
     2581 aacaatgctc cgtctggcta aagaaaaaaa gcaattagcc attattaatg atcaatatgg
     2641 cgcccctact ggagcagaat tgctggcgga ttgtactgcg attgcaatcc gtgcagcagt
     2701 aaacgatgtt tcgctatatg gtacgtatca ccttgttgcc agtggggaaa ccacctggta
     2761 tgactatgca caatatgtgt tctctgttgc tcgcgaactc ggtgaacccc tttctgttga
     2821 ggaagttaaa ggtgttgata caaccgcgta tccaaccccc gctaaacgtc catcaaactc
     2881 tcgcttgtcg aatttaaaat ttcagactgc ttttgctact gttattcctg actggaaagt
     2941 gggtgtgaag cgtgttgtga gtgaagtgct tggaaagtaa ataaatgaac gtaatcaaga
     3001 ctgaaattcc agaagtatta atttttgagc cgaaagtatt tggtgatgca cgtggttttt
     3061 tctttgaaag cttcagtgaa aaaatttttg aagaagccgt aggggaaagg ataaaatttg
     3121 tgcaggataa tcattcacaa tcacaaaaag gggtactgcg cggtttacat taccagcttg
     3181 atccacatgc gcagggcaaa ctggtacgtt gcattgaagg cgaagtattt gatgtagctg
     3241 tcgatatccg aaaaaaatct gcaacttttg gccactgggt cggggttatt ttaagcgctg
     3301 aaaataagcg acaactttgg atacctgaag gattcgctca tggtttttta acactaagcg
     3361 aaactgcgca attcgtatat aaagcaacaa attattatgc accacaatca gagcgttgca
     3421 ttatatggaa cgatccgcaa atcaacatcg aatggccaga gcgggatcaa gtacaactgt
     3481 cggcaaaaga tgaaaaaggg cttctcttat caggtgcgga aacctatctc taacatgaag
     3541 atggataaga gagtcgcaat actattgtgc acttacaacg gaagtaagtt tttacgcaaa
     3601 caacttgact caattacaaa tcaaacccat cagagctggg ttatctttgc atctgacgat
     3661 ggttctactg acgatacgtt ggatattatc agagaatatc agcaaacata tggaggagac
     3721 agggttgtct tactgcgtgg tcccggtaaa ggttttgcat ggaattttat tagtgcactt
     3781 gagtcctgtg gcgagggttt tgattattat gcctttagcg atcaggatga cgaatggatg
     3841 gaaaacaaat tatcacatgc tctgagcttg ttgaatgata atccacaaaa gcctgttgtc
     3901 tattgcggta gaacattact gacaaatgaa gaaggggatg agattggttt ctcccctctt
     3961 tttgataaaa gaccgtcttt tcgtaatgca ttagtacaga gtattgcggg tggcaatacc
     4021 atggtactca ataagcaagc cagagatatt gttataaaaa cgcctaaatg gcaggaaatc
     4081 gtttcgcatg actggtgggt ttatatactc attacaggat gtggaggaga gatatattac
     4141 gatcctttgc caatgataaa atatcgacag caccaggaaa acattattgg atcgaatctc
     4201 tcaatgttag ctcgcattca gcgaatcaaa aagctgatgg ctggccattt taaacgctgg
     4261 aatgataaaa atattgagct gcttaagccc ttcacagaca aattaacgcc ggaaaatgcc
     4321 agaattttaa acagctttga taacaatcgg aacgcgaaca tttttttacg catcaaaatg
     4381 atttttgaag caaaattata tcgccaaaca attttcggaa atattgcatt aatcgttgct
     4441 attattttca aaaaaattta acggcaatag acaatttatt tttgaggtag agatgtgagt
     4501 ggaaagctaa ataaggggtt atcgggttcg cttataaact cgttatggag taaccgcagc
     4561 cttatttttc agatgacgaa aagggatatt ttaagtaagt ataagggatc ggcgattggt
     4621 gttgtttggt ccctcattaa ccccatcctt atgctaacta tctataccct tgttttttct
     4681 gttgttttta aagctcgctg gggcaatgcg gcagtagatc aaccaaagac gcaatttgct
     4741 gttattttgt ttgtagggat gattattcat tcatttctgg ctgagtgctt agttagagcg
     4801 ccaaatctta tcttacagca tgcgaattac gtcaaaaaag ttgtatttcc actagaaata
     4861 cttcctgtgg ttaccgtctt gtctgcaata tttcaaagtg ttattagtgg tactgtcctg
     4921 attatggctt tttttgtatt taatggttac gttcactgga ccttgatatt tgcgccatta
     4981 gtgtttctac cgctagtaat tttagcgctt ggtttgacct ggcttatatc ttccttagga
     5041 gtatatgttc gtgatatcgg tcaatctatc ggtgttatag taactgtctt aatattcctc
     5101 tctcctgttt tttatcctct ttccgcttta ccacagtgga tgcaatcaat tgttttgctt
     5161 aacccattaa cttatattat tgagcaatca cgtgctgtta ttgtatgggg acaattacct
     5221 gatttcctgg gcttagcaat atattcagtg aatgctcttt ggattagcta cgcttgctat
     5281 ttctggttcc agaaaacaag gggaggattt gctgatgtca tctgatttat ttgccattga
     5341 agttaataac gtcagcaaat gttatcaagt atatgataat cccctaaaac gattaaaaga
     5401 aattattata ccaaaaaaac aacagcgcga aggcaaagtc gaacgattgt accatgatga
     5461 gttttgggcg ctgcaggatg ttagctttcg ccttaaaaaa ggtgaaacag tggggatcgt
     5521 tggtaaaaat ggctccggta aatcaaccct tttacaaatt attgccggta cactgacgcc
     5581 tactaccggt agcgttaaaa ttgctgggcg ggttgctgcc ctgcttgaat taggcgccgg
     5641 ttttaataat gattttacgg gtcgtgaaaa tatttatctc aatgcagtat tgctcggtct
     5701 gagcaaagca gaaatcgatg agaaaataga tgatatcatt agttttgcag atatcggaca
     5761 ttttatcgat tcgccggtac gatcctattc aagcggcatg ttggttcggc ttgcgtttgc
     5821 tatacaagcg cagatagaac ccgagatcct gattgtagat gaagctttag cagttggcga
     5881 tgcaagattt caggcgaaat gttttgcgcg cttaaagcag ttaaaagaaa gaggaacttc
     5941 gattctcttt gtttctcatg caacagaaca gattgtaacg cattgtgatt ctgccatttt
     6001 aattaatgat ggagtcatgc tcatgcaaga cagtcctcgt gtaatagtaa ataaatatct
     6061 cgatcttctt tttggtaaaa cagataaaaa aattgaagtt aaagaagaga ctgagtccag
     6121 tattgaacaa caccgtgctg aaggccattt gcaagatata tcgaataatc tgtttgcttt
     6181 cgaggatggg ttgagttttg aacaacgacc taattacaat ccttcagaat atcgctgggg
     6241 agatctgaaa gcagaaattg tcgattacca gctgattcag gatggtaaac actatccgct
     6301 ggtcattgag agcgaacatg ctattcatct gcaattttat ataaaattta aagatgccgt
     6361 ctatcgtccc atctttggta ttgcacttaa aactaaagaa ggggttacca tttataatac
     6421 taacagtgag taccaagata ttgatatgtc cagacaattc aatgctggag atatcatgca
     6481 cgcttctttt tctatgcctg ttaatcttgc atccggcgat tatttcatct cggtgggcat
     6541 tgcaagctgc gatgaaggcg gagcggtcgt tcctcatgac agacgttatg actctataca
     6601 tttgagtgtt gtgaaccgag tgcaatttat cggcctggtg aatttagaca ctcatattag
     6661 tctgaaataa aatagtttca ttgcagagta acacacaagc gatgaagcaa ttacaggtga
     6721 gttttatgtt tcaacttgaa agtctaggtt atttttacga taatagcctt aatgtatgga
     6781 agcgggaaga ttacattaac attggttata acgatggtga tgaagcagaa gaacgtctgg
     6841 ctaaaatcat tcgtgacgcg aaagacatca gcctcttttc atctgaatta cgtaataaat
     6901 gcgctgactg gcaaacgctt tatcacctga cttctcaacg cggtaatgta ctgcgtcctt
     6961 tcgcgcattt gctgaaaggt gatgtattgg aaattggcgc aggatgcgga gcgattagcc
     7021 gtttcttagg tgaaaacggc gggaacatcc tggcgctgga aggtagccag cgtcgtgcat
     7081 ctattgctgc gagccgcact cgtgatttgg ataacgtgca ggttgttgcg gagcgtttcg
     7141 accagtttaa tccgggacga caatttgatg cgattactct cattggcgtt cttgaatatg
     7201 ctccgatgtt tggtaatgga gaatctccag aaaaggattt acttattcag gttcgcaaat
     7261 tgcttaagcc tggaggtttt ttattcatcg ctattgagaa taagttaggt cttaaatact
     7321 ttgctggcgc gcccgaagac catattcgac aatctatgta tggcattgaa gggcgatata
     7381 aatctggaga agcgaaaacg tggggttatg aagaactcaa acaattactc actgaaactg
     7441 gttatcgcta ttcagatgta cttctgccat ttcctgacta taaattacca acatcaatta
     7501 taactccagc aggaagtata acgacaggtt ttgacgcctc tacgcttgct gtgcagagtg
     7561 taatgagcga ccctcaatta cccgcgcatc taacattctc cctgcaaaat tgttttcctg
     7621 aaattttcaa aaataagctg ggtgtttcgc ttgctaattc atttattttc gccgcgagca
     7681 tagatactga aataccgtta aacgattcca ttcttgcata tcattacagt acgcaacgta
     7741 agcatcaata tgcaaaagaa acggtattca gtcaaaatag taagaatgaa atcgatgttg
     7801 cttatcactt tttccaaaag gaatatcgtg aacctgatac aaacagatac gttcagataa
     7861 gaaaagaagg tgataaatat tttcgaggaa ctctacttgg tcaacagttt attaatgtgc
     7921 tcgctaatga aaattggcag tttgatgata ttatcaagtt aatgaagcag tggcttagtg
     7981 ctctggaaaa gtttattaag aatgaaggcg ataagcttga gttttcttgt caggttgatt
     8041 ttagtctgcc tgcatatttt atggatgcaa ttccgcaaaa tattatcata acagatgaag
     8101 gacctgttct gtttgatatt gaatggcagg gaattgacag gattgaatta ggtcaccttc
     8161 tgtttagagg tattctttta atactcaatc aagccaaagt gcggccactt gtcggacaag
     8221 aactcattac tcgtaaagaa ttcgtacaaa aagttatgac atctttggga ttagttttta
     8281 aagaagatac tctacaaggc tatatacgca aggaatctca atttcagcat caggtcacgg
     8341 ggcgtgatat taacgagtta ttgctctgga atgaagaacg ccctcttgcg cctgttacgg
     8401 taaagcacag ccatgcttgt ttatactttg catcaaatgg agaagagttt actgaacaga
     8461 atacgcactc gtttattctg gcccccggta taaatacgat tcgctgtcag gttaatggta
     8521 acaatcagtt acccattacg aaattacggt ttgatccgac ggatagctct ggttggttta
     8581 cgatacattc attgagtatt aaaaataatc atggagaggt tgtttggcaa gcgggggcgg
     8641 acagtgagga tgttgattac caggatattc ttaagcttca ggaaggtatg ctgaatgttt
     8701 attactcgac ctcacacgat ccacaaattt tattaaaaaa tgtacctcct ctcacagaac
     8761 tctatttgga agctgagctg gaaattattc aaaacatgtc tttggcttca ttcctggaag
     8821 agaataatac tcatccttac tcgcctgtat taaaaaaact gaacgaaata tccgcacagt
     8881 tagaagagac gtgtttaaaa ctggccaaaa cggattccct aaataatgat gtggaagcaa
     8941 aattgagaca gaccgaagat catttagatt caacaaccgc gcaattaaat gaaaaaagca
     9001 ctcagttgga aaatatacag agccagctca ctgcagtaac gtcacaactt actcatgtaa
     9061 atgctctaca cgccgccgca atgacgagaa taaatgcatt agaagcatcc accagttgga
     9121 aagttactgc gcccctgcgt gctctcggta aagtaagaca ccgaactaag gtgttggctt
     9181 cagttacctg tcgcatatat cgccaacacg gcttaagaaa aattatctct cgtggctttg
     9241 ctacgctgca acgcgaaggt tggaggggga tcgtcggacg gcttcgtcag cagcgtatga
     9301 tgcaatcatt tcatgccgcc gctcccttta cgctaaatgc tgaaaatgct gttaccgcac
     9361 aaggcaattc agtctctgca ccaacggtgc aaccaactac tatcgtttat aatcagcgtg
     9421 aaggatatca tttaaagcag ggggagcatg agtattgtta tgtcgtgccg gcacaacctg
     9481 atgggctggc aaccttaatt agtgatatga cgatcaaacc gctgttttcc attgtggtgc
     9541 cgatttacaa cactccattg gatctgttgc atgagctcat agaatctatc gaagcgcagt
     9601 ggtatcccca atgggaactg attctggcca atgactgcag tacagatccg cagctgtatg
     9661 gggcactgga aaagataact aatcctaaga tcaaaattgt tcatcttaag acaaacagcc
     9721 gtatctccgg tgcgacaaat gtcgctattg aacatgcaac aggtgactac attgtcttta
     9781 cagaccatga cgatctttta acgcctgatt gcttatatga gatggctgtc tgcattaata
     9841 atgaagatcc agattttatc tacagcgacg aagataagct ctccgaagac gggcgttacg
     9901 ttcaaccgca cttcaaacct gactggtcac cagatactat gatgagtaca atgttcactt
     9961 gtcatgcctc ttgtgttaaa aaatcattat tgtcaaaaac agggttattg cgctctgaat
    10021 ttgatggttg tcaggactgg gattttgtat taagagtggc agagcacaca aacaaaataa
    10081 gccatattgc aaaagtactt tatcactggc gtataatacc tcagtctatt gcttcaaatc
    10141 tggcggctaa agaatatgtt cttcaggcat cgataaatgt tcgtaaagat gccttaaaac
    10201 gtagaggctt agcgggcgag gtcgaatcaa ttaaagatta tccaggctat tttagggtaa
    10261 actacttccc aattaataaa cctttagtaa gtattataat cccaacgcgt gataattata
    10321 atgttttgag tcgctgtatt gattcacttt taaagggaac aaaatacgac aattatgaga
    10381 ttatcatact tgataatggt tcgacttcac cagagacgct atcctatctc gaacagttaa
    10441 gtaagctgga taaaataaaa acaataaggc atgatcatcc ttttaatttt tctgaattaa
    10501 ataacattgg cgtgagtaac tcgcgtggag atgtattgtt attcttaaat gatgatactg
    10561 aaattatcaa tgatgattgg ctcgaacgca tgattggata cgctcaactt ccacatattg
    10621 gggcagtagg agcaaagtta tactatccaa ataagaagtg cgtacaacat gctggaatcg
    10681 ttaatctcgc tgacgggccc ggtcatgcga tgttaggctt aaatgaagaa gagccaggct
    10741 atttcatgcg aaataaatta gaatataatt ggatagcagt tacgggtgca tgtcttatga
    10801 ttgaaaggca taagtttgag aaaattggac ggttcgatga agattttcct atagcctata
    10861 atgatgttga tttgtgcttt agactcattt cttcgggata ttataatgtc gtttgtcagg
    10921 ctgttgaact ttaccatcat gagtcagtta gtcgaggtgt agatcacata gatgttgaaa
    10981 aggcccaacg actccatatg gaaaagcaac gtctttatca aaaacatcct cgttattatc
    11041 aatacgatcc attctttaat attaatttgc acccgaatgg cattaatttt gaggtaggtt
    11101 gctgatgctt atgaacacca tttctgggcc tgaatttaat cgctatgatg ctgcaattaa
    11161 atattttgaa gacatgccag ttgacgcgtt gcgaaatgcc aaagttatct cctttgattt
    11221 tttcgatacg ctatttaccc gaccactcgc cgatcctgaa gatgcctttg atattcttgg
    11281 aaaacaactg gctatcgata atttcaggaa gaagagacag ctcgcgcaaa tagaggcatt
    11341 tcgtcatatg agcgcaagcg gaaaaaaaga aatcaatatc gaaggcatct atgctgcctt
    11401 tgctgataac tcccgcactg cagaagaaat ggcaaatgca gaatatcaac tggaacttaa
    11461 actggttgag ccaaacacaa taatatttga ccttttccgg agcttgaaag atgccgggaa
    11521 aacggttgtg gtaacttccg atatgtacct acctgcgcgt ttttttgagc aagcgctgga
    11581 aaagttcggt gttactggcg tagccgtatt tagttcttcg gatgaaaacg ccacaaaacg
    11641 agattcgggt gaaatatttg atgtgatcgc gcagaagcta caggtggcgc ccggggatat
    11701 tcttcatctt ggtgataatg aaatttctga cgttattcgt ccgcgtgaga aaggcgttaa
    11761 ggcttatctc taccgttcac attatttagg gacaatttgc cagggactcc cgctctctct
    11821 ttctattgcc caagggatgc ttaaaagcca tgacgattac catgatctcc aggcgtttaa
    11881 aagcctgggt tttgcttatg gtggcgcggc gtcgcttggc ttccttaaat ggattaaaaa
    11941 tcagactgtc ttgcatcaaa tcgaccatgt gctctttttg tcacgcgatg gatacgtgat
    12001 gcaaaaaatg gttgaacggg gtgatgttgg ggattttcct cgctccactt acttttatgg
    12061 ttcacgtatt gcgcttacgc ttgcgaccat caatgaccaa aactttgttc attacattcc
    12121 atttttactg tctggtgctg agggtttgca accctgcgag atccttgagc gtataggcgt
    12181 ggcggcaccc gcacccgaag ttatggcttc gtttggttta tcagcagaaa ctccactaac
    12241 tgctggactg ttttcgaaaa tggccaactt tttatatgcg tggagatggc agattttgct
    12301 ggtttgccgt agaaaccggc gtggcctctt tatgtacttg cagcagttag gaattaaaag
    12361 tggtgagaga attgctctcg tcgatgtggg ctggagcggc acgacacaag aggcttttgt
    12421 gcatgctgtg aaacagttta tggatattga tattcatggc tactatttct gtctggcaaa
    12481 cacggcagag aaaaacagaa gaaaagcgtt tttgcaaatg cacgcgatgt attcaactga
    12541 gaatgctgat aattctatta tagatatgat ctatgaaaac cgcgttgcag ttgagctttt
    12601 tttctctgcg ccacacgatt caattattgg ctacgaagcc tttactggaa agccagtggg
    12661 tgatcgtggt cgcggcctaa aaaacgattt aacgcatagt atctcagata tatgtgccgg
    12721 cgcgatgtta tttgttaaaa gttataatga actctgcaag cgcctaagtc ttgaattaac
    12781 ccctcatgaa cttacgatga gtatgcttag cttagttagt aataaaaaat ggcaggatta
    12841 ttcagcaatt acgaacatct caaattttga tgcgtgggga agctcacgat taaaatcgct
    12901 ttgcgcgaaa gattatttga atagttaaat gaactattta ctttcattta ttgcccctct
    12961 gttagtttaa cgcctgctgt attgctctta tttataaata agagcaatgt taaattaata
    13021 atgcagtgca ataggtttgt gaaaattata ataaatgtag tgacttagtc tactgtttaa
    13081 agaagtttgt gaatatcact ctcttatcag ttatacgttt acgcaagtgc tatgatttat
    13141 tgcatcttta tatataatta ataataaaaa ttatccaggt atcgtgatgc ttgttatata
    13201 tcaatgatgc ttgtgctacc tggtttcaag gattccaact catagcaaca tggcaaaata
    13261 gtgtcgtttt aatacatgct atctacggat taggaccagc caatcattct tgcgtgggta
    13321 cccagcgtgt taaatctacg ttactataat ccttacttaa gccctctggc ctggctgtca
    13381 gagggttacc acacctgaca ggagttttgc atgtctaagc aacaaattgg cgttgt
//
