LOCUS       AB812043               14195 bp    DNA     linear   BCT 13-MAY-2015
DEFINITION  Escherichia coli genes for O-antigen biosynthetic locus, partial
            sequence, strain: H319.
ACCESSION   AB812043
VERSION     AB812043.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Iguchi,A., Iyoda,S., Kikuchi,T., Ogura,Y., Katsura,K., Ohnishi,M.,
            Hayashi,T. and Thomson,N.R.
  TITLE     A complete view of the genetic diversity of the Escherichia coli
            O-antigen biosynthesis gene cluster
  JOURNAL   DNA Res. 22 (1), 101-107 (2015)
   PUBMED   25428893
  REMARK    DOI:10.1093/dnares/dsu043
REFERENCE   2  (bases 1 to 14195)
  AUTHORS   Iguchi,A., Iyoda,S. and Ohnishi,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2013) Contact:Atsushi Iguchi University of
            Miyazaki, Faculty of Agriculture; Gakuen-kibanadai-nishi 1-1,
            Miyazaki, Miyazaki 889-2192, Japan
FEATURES             Location/Qualifiers
     source          1..14195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="H319"
                     /db_xref="taxon:562"
                     /note="serogroup:O96"
     gene            <1..906
                     /gene="wcaM"
     CDS             <1..906
                     /gene="wcaM"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein"
                     /protein_id="BAQ01414.1"
                     /translation="NYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILIS
                     DHVIERINCTNGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENG
                     KHFVIRNIKARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGK
                     YLSIPQNFRVNNIQLDNTHLAYKLRGIQISAGNAASFVALTNIEMKRASLELHNKPQHL
                     FMRNIKVMQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDR
                     VNHHIVNVEKINFRLPERRE"
     gene            1069..1974
                     /gene="galF"
     CDS             1069..1974
                     /gene="galF"
                     /note="O096_01"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase"
                     /protein_id="BAQ01415.1"
                     /translation="MDEIMTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIV
                     DEIVAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIM
                     NVRQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGR
                     SQVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKYG
                     LRNLKEGAKFRKGIEKLLSE"
     gene            2309..3556
                     /gene="wzx"
     CDS             2309..3556
                     /gene="wzx"
                     /note="O096_02"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P37746|2.A.66.2.1"
                     /protein_id="BAQ01416.1"
                     /translation="MKINTTLNKNILYLAVVQGSSYILPLITFPYLVRVLGPELFGVLG
                     FCQASMQYLVLLTDYGFNWTATQQVAKNKNDIVKLTRIFWSVFFAKVFLASISFILLAA
                     CCFLIERYKELWFVLFSFSPLVLGNVIYPVWFFQGMEKMKWITICTITARCLVIPLTFI
                     FVKNGQDVWVAALIQGMVNLLAGLLGLCLIKKKRWVNTIIVDYIDIKQCLKDGWHVFIS
                     TSAISLYTTSTTVILGFVAGPLAVGYFNVANTIRNAAQGLLTPFTQSIYPRINAVFDSD
                     YLQAIKIIKKSLRYVGGLAFLGSVFLYLLAPFIIKIGVGGGYEESISVLRIMAFLPFII
                     VLSNIFGVQTMLTHNYKRQFSQILLVSGVANLIIIYPLVIMYSANGAAISLLVTELFVT
                     AQMYLFLRAKKIYLIK"
     gene            3568..4665
                     /gene="glf"
     CDS             3568..4665
                     /gene="glf"
                     /note="O096_03"
                     /codon_start=1
                     /transl_table=11
                     /product="GT2|GT2_Glycos_transf_2"
                     /protein_id="BAQ01417.1"
                     /translation="MYDYLIVGAGLFGAVCAHELNKKGKKLLVIDRRDHIAGNAYTENN
                     GGIQVHKYGAHIFHTNDKDIWNYVNSFVEFNRFTNSPLASYGDKLYNLPFNMNTFYQLW
                     GVKTPAEALAIINEQRKVLGDKTPSNLEEKAISLVGFDIYEKLIKGYTEKQWGRKATDL
                     PSFIINRLPVRFTFDNNYFSDLYQGIPIGGYTKMVEKMLDGIETRLGVDFLKNRDSFRK
                     IADKIIYTGPIDEFYDYCFGMLEYRSLRFENEVINSNNYQGNAVINYTESHVPYTRIIE
                     HKHFDPIETNHTVITREYPCEWKKGDEPYYPVNDNLNMELFKKYKQLANSENNIIFGGR
                     LGEYKYYDMHQVIKSALNLMVKLDK"
     CDS             4662..5576
                     /note="O096_04"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ01418.1"
                     /translation="MKVQHRVAVVLVTYNRVALLDRAIQALYAQTYPVHKIIVVNNAST
                     DDTANYLAQKNDIVHLQLPKNTGGAGGFHEGIKKACSMDVDFIWVMDDDAVPNNNTLKG
                     LINDAQFLEASGDNWGFLCSKVVSEDNESINVPSISRKRNRSGYLSWSEKAENGLIGVD
                     TATFVSVLMKKEIPFLVGLPVKEMFIWGDDTEYTWRISTLKNCYLSANSTIYHKRISAS
                     ALSVVTEKEKTRLNWYIYYYRNNLYARRKHGSKKDMLVFLISTIKDIKDIFCKSKNNKL
                     LRLKCLLIGIFKGVVFNPQIKNV"
     gene            5614..6864
                     /gene="wzy"
     CDS             5614..6864
                     /gene="wzy"
                     /note="O096_05"
                     /codon_start=1
                     /transl_table=11
                     /product="GT2|GT2_Glycos_transf_2"
                     /protein_id="BAQ01419.1"
                     /translation="MTSSNKILSLLFGVILFLPLFPVQLNETQYHVTLAILLGLILIFL
                     MSWKQLGGFKGGSPVLYYYLCLQLLLILSGVMGVDTVKEYQDLISLFRPMVLLSSFYLL
                     YCLTSRNRDFFRTLIKPMKFICMAMFIWAVYESIIQNNIFQSINYLLYKMERKDDIKNV
                     AVTFFFLPYYSAFVSVFMIAFFYTCRYTYKIRGTYKYIFMSFCCIVLTQSKTGVVAALF
                     LIVCCELISSSQLKRIFCAVCVIILFFISFYFYDYLLMYLNLYFPGNFTRTVSQLADDP
                     SSAYTLSARIEQFLSIYSQTMQHIPFIGYGLGRDLLIESWPATIYVRYGWIGICVFLIF
                     NFFLIFKGICYYLTHTSNAFRYQAIIFSFWIISTLISQMSAMMTENTKTSFIYLVYLVI
                     FMQLIYKPHKINKSNLG"
     CDS             6869..7990
                     /note="O096_06"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ01420.1"
                     /translation="MGKHALLVNGSYLPTVGGVENSIRSIAAELVKEGWKVDVVCSDEG
                     KYSRYEKTNNVSVYRYAKKGFFRSLYNSFRVLKEINKKYQLIICRNHTLFIILKFAGYK
                     NIHYIIPGVYHYQNKSEMRGSLSKKIKYASNVFIQIVSFNISKLKYVFSPTMEDQVCSV
                     SIFNKSVKKIYPGVDDTRFYKLDRKLKNNLKLALGFDNDSKIVLGLGRFVDVKNFEHLI
                     KCVPYLPCDYKVVLVGGGDNLTVYKKIIGELNLINRVFIFESTLEPEKFYQIADVFCLT
                     STYEPFGQVLIEASFCGLNIVAFDSDLKGIDTATNSIFIHDCDFYFPVSSFELSDFAKE
                     IINAVTCNRSSLCQNEFNKQYNWKALIERLSID"
     CDS             7968..8930
                     /note="O096_07"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ01421.1"
                     /translation="MRDYQLIKYSFIIPCYNVSKYILTAIESIPVREDIEIIIIDDGST
                     DGLSNAISSYKGNKIIYFHKENGGVSSARNKGIDLATGKYLLFFDGDDYYSEDLINYLD
                     KGFDNAQAKMITFGYNHLANNRLKYYSPGTTGIKSTRVLIGQILLRQSFQCMCSFAVES
                     DLVKNNNLRFNEVTYYYEDIEFQLKVMSLMNFVFVIDKPLFFYISRKKSATNSFVNEKH
                     FSLFYAIDRLENILDADMNDSLTYFRWYSLFWIFRLAFKNGCTKKSISILKTLKLSNFK
                     YNLIPCSQGRFKYNLIWLFQKFPIPFISFILNLRAIRNN"
     CDS             8945..9868
                     /note="O096_08"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="BAQ01422.1"
                     /translation="MNTHKVIAVIVTYRRKNFLDKVLSALLNQTVPLHKVIVVDNNSSD
                     GTDDIVKEYINKFPEVISYHNTHENLGGAGGFYTGMKLIEKYNYNYDYAWLMDDDLIPN
                     NDCLEIMLTANIKGIIQPMRFNLDGSCAELSALKYDLNSFFRLKPKGDTVKDFVASNPL
                     LPDFINIETVPFEGPLIHKDIVTAVGLPDPRFFIFGDDTDYSIRTLKLNYPIICMPRAR
                     ASRLLINNQRNDLLSWKGFFMLRNLFHLYFKHGNGYIARTKPYIIAVCYLLLCLVKGNF
                     KQSLITVDALKSARLLMNNDKYKPKA"
     gene            9987..11393
                     /gene="gnd"
     CDS             9987..11393
                     /gene="gnd"
                     /note="O096_09"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="BAQ01423.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIAE
                     NPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGNTF
                     FQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIAAVA
                     EDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQTFTEW
                     NNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEPLSLIT
                     ESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYAQGFSQL
                     RAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPYFKQIADD
                     YQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGAHTYKRIDK
                     EGVFHTEWLD"
     gene            11642..12808
                     /gene="ugd"
     CDS             11642..12808
                     /gene="ugd"
                     /note="O096_10"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="BAQ01424.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSPI
                     VDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIRDV
                     TEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVIGER
                     SERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYAESLG
                     LNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAIVDANR
                     TRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVIIYEPVMK
                     EDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            12898..13932
                     /gene="wzz"
     CDS             12898..13932
                     /gene="wzz"
                     /note="O096_11"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen chain length determinant protein"
                     /protein_id="BAQ01425.1"
                     /translation="MAIIAKLWRLKIISSVRVMMRVENNNVSGQNHDPEQIDLIDLLVQ
                     LWRGKMTIIISAIVAIALAIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGSAA
                     PKVSEIQSMLIGRFSTTFSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETPEGA
                     QKQLAQYIQQVDDQVNEELEQDLKDNIALQMKNLQDSLKTQEVVAQEQKDLRIRQIQEA
                     LQYANQAQVTKPQIQQTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQTRQNLL
                     DIDNLDVDKLDIHAYRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRN
                     YNAK"
     gene            complement(14029..>14195)
                     /gene="hisI"
     CDS             complement(14029..>14195)
                     /gene="hisI"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="BAQ01426.1"
                     /translation="VGEEGVETALAATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTV
                     IENLRKRHQ"
ORIGIN
        1 aattacgcca tcttacgcca gggatttcat aaccagataa tcggtgccaa catcaccaat
       61 tgtaagttca gcgacttaca gggcgatgcc attgaatgga acgtggcgat taacgaccgt
      121 gatattttga tctccgacca tgtcatcgag cgcatcaact gtactaatgg caaaatcaac
      181 tggggcattg gcataggcct tgcgggaagt acctacgata acaactaccc ggaagaccag
      241 gcagtgaaaa actttgtcgt ggcgaatatc acgggatcgg attgtcggca gttgatccat
      301 gttgaaaatg gtaaacattt tgttattcgt aatatcaaag cccgcaatat cacgccggat
      361 ttcagtaaga aagcaggcat tgataacgcc acagtcgcta tttacggttg tgacaatttc
      421 gtgattgata atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt
      481 aaaggcaaat atctctcgat accgcaaaat ttccgagtga ataatattca actggataat
      541 acccaccttg cttacaaatt gcgcggcatc caaatctccg ccgggaatgc tgcctccttt
      601 gtggcactga ctaacattga gatgaagcgt gcctcgctgg agcttcacaa caaaccgcaa
      661 catcttttta tgcgtaatat caaggtgatg caggaatcct ctgttggacc cgcattgagc
      721 atgaacttcg acatgcgcaa agacgttcgc ggcgtcttta tggcgaaaaa agaaacactg
      781 ctgtcacttg caaatgttca tgcggtgaat gaaaaagggc aaagctccgt cgatatcgac
      841 agagttaatc accatattgt taatgtggaa aagattaact ttagattgcc ggaacggagg
      901 gagtagattt gcgaccattc ctggaaaaat ggagccatac ttaggaacaa tgctactgca
      961 atccacaacg aagcggcgta acatcataag taattcagta atcaattcag ggtaattgat
     1021 gctggcgaaa aaaatcgaac aagctataat tcagcaacca ttttacaggt ggatgaaata
     1081 atgacgaatt taaaagcagt tattcctgta gcgggtcttg ggatgcatat gttgcctgcc
     1141 actaaggcga tacccaaaga gatgctaccg atcgtcgaca agccaatgat tcagtacatt
     1201 gttgacgaga ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcatccaag
     1261 aacgcggtcg aaaaccactt cgacacctct tatgaattag aatctctcct tgaacagcgc
     1321 gtgaagcgtc agctgctggc ggaagtgcag tccatctgtc cgccgggcgt gacaattatg
     1381 aacgtgcgtc agggagaacc tttaggtttg ggccactcca ttttatgtgc acgacctgcc
     1441 attggtgaca atccatttgt cgtggtgctg ccagacgttg tgatcgacga tgccagcgcc
     1501 gacccgctgc gctacaacct tgcagccatg attgcgcgct tcaatgaaac gggccgcagc
     1561 caggtgctgg caaaacgtat gccaggtgac ctctctgaat actctgtcat ccagaccaaa
     1621 gagccgctgg atcgcgaagg taaagtcagc cgcattgttg aattcatcga aaaaccggat
     1681 cagccgcaaa cgctggactc agacatcatg gccgttggtc gctatgtgct ttctgccgat
     1741 atttggccgg aactagaacg cactcagcca ggtgcatggg gacgtattca gctgactgat
     1801 gccattgccg aactggcgaa aaaacagtcc gttgatgcca tgctgatgac tggagacagc
     1861 tacgactgcg gaaaaaaaat gggctatatg caggcgtttg tgaagtatgg tctgcgcaac
     1921 ctcaaagaag gggcgaagtt ccgtaaaggg attgagaagc tgttaagcga ataatgaaaa
     1981 tctgaccgga tgtaacggtt gataagaaaa ttataacggc agtgaagatt cgtggcgaaa
     2041 gtaatttgtt gcgaattttc ctgccgttgt tttatataaa caatcagaat aacaacgagt
     2101 tagcaatagg attttagtca aagttttcca ggattttcct tgtttccaga gcggattggt
     2161 aagacaatta gcgtttgaat ttttcgggtt tagcgcgagt gggtaacgct cgtcacatcg
     2221 taggtatgca tgcagtgctc tggtagctgt aaagccaggg gcggtagcgt gtgtaaaaaa
     2281 cacattaaca ttattctgat ggatacgtgt gaaaataaat acgactttaa ataaaaatat
     2341 tttatattta gctgttgttc aagggagttc atatattctt ccattaatca cttttcctta
     2401 tttagtgaga gtgttaggtc ccgagttatt tggggtgcta ggtttttgtc aggcatcaat
     2461 gcaatacttg gtgttattaa ctgactatgg atttaattgg acggcaactc agcaagtagc
     2521 taaaaataaa aatgacatcg taaaattaac taggattttt tggtctgttt tttttgcaaa
     2581 agtattcttg gcaagtattt cgtttatact tttagctgca tgttgttttc ttattgaaag
     2641 atataaagaa ttgtggtttg tattattttc atttagtccc ttggtattgg ggaatgtgat
     2701 ttatcctgta tggttttttc agggtatgga aaaaatgaaa tggataacta tatgtactat
     2761 tacggctcga tgtttagtga tacctttaac atttatcttt gttaaaaatg gacaagacgt
     2821 atgggttgct gcacttatcc aggggatggt taacttatta gcaggcctac tgggattatg
     2881 tttgattaag aaaaaaagat gggttaatac gattatcgtt gattatattg atattaaaca
     2941 gtgcttaaag gatggttggc acgtgtttat ttcaacatca gctataagtc tttatacaac
     3001 aagcacaaca gtgattctcg ggtttgtggc gggacctttg gctgtagggt attttaatgt
     3061 tgcaaatact atacgtaatg ccgcgcaagg gttgctaaca ccttttactc aatctatata
     3121 tcctagaatt aacgcggtct ttgatagtga ttacttacag gcaataaaaa taataaaaaa
     3181 atcgctacgc tatgttggag gtttagcttt tttgggatca gtattcttat atctattggc
     3241 tccatttata ataaaaatag gtgtcggagg ggggtatgaa gagtcaatat ctgttttgcg
     3301 aataatggca tttttaccat ttattattgt gttaagtaat atatttggtg ttcaaacaat
     3361 gttgactcat aattataaga gacagtttag ccaaatactg ctagttagtg gcgttgcaaa
     3421 tcttataatt atttatccat tagtaataat gtattctgct aatggtgctg caatttcttt
     3481 attagtaacg gagttgtttg ttacggctca aatgtactta tttttacggg ctaaaaaaat
     3541 atatttaata aagtaatagg agatgatatg tacgactatc ttattgttgg tgcggggttg
     3601 tttggggctg tttgtgcgca tgagttgaat aaaaaaggga aaaaattatt ggtaattgat
     3661 cgaagggacc acattgctgg taatgcttac actgaaaata atggtggtat tcaagttcat
     3721 aaatatgggg cgcatatatt ccatacaaat gataaagata tatggaatta tgttaatagt
     3781 tttgttgagt ttaatcggtt tactaattca cctcttgcta gttatggaga taaactttac
     3841 aatttaccct ttaatatgaa tacgttttat cagctttggg gagttaagac gcctgctgaa
     3901 gcattagcta taattaatga acagcgaaaa gttttaggtg ataagacacc tagtaatctt
     3961 gaggaaaagg ctatttcttt agtcggcttt gatatttatg aaaaattgat caaaggatat
     4021 actgaaaagc aatggggaag aaaggccact gatcttccat cttttattat caatcggttg
     4081 ccagtacgat ttacttttga caataattat ttttctgatc tttatcaggg gatacctatt
     4141 ggtggttata ccaagatggt ggaaaaaatg ttagatggta tagaaactag gcttggtgtc
     4201 gattttttaa agaatagaga ttctttcaga aagatagctg ataaaattat ttatactggg
     4261 ccgatagatg agttttatga ttactgcttt ggaatgctcg aatatagatc tttaagattt
     4321 gaaaatgaag ttattaatag taataattat caaggaaatg ctgttataaa ttatactgaa
     4381 tcacatgtac catacacaag aataatagaa cacaaacact ttgacccaat tgaaacaaac
     4441 catacagtta tcacaagaga atatccttgt gaatggaaaa aaggtgatga accttactat
     4501 ccagtaaatg acaatctgaa catggaatta ttcaaaaaat ataaacaatt agcaaattct
     4561 gaaaataata tcatttttgg tggacggctt ggggagtata aatattacga tatgcatcaa
     4621 gtaattaaat cggcattaaa tttgatggtg aaattagaca aatgaaggtt caacatagag
     4681 ttgctgtcgt tttggtaaca tataaccgtg ttgcattgct agatagagct attcaagcat
     4741 tatatgcgca aacatatcca gtccacaaaa ttatagttgt aaataatgct tctactgatg
     4801 ataccgcaaa ttatttagca caaaaaaatg atattgttca cttacaactc cctaaaaata
     4861 caggtggggc tggtggtttt catgaaggta taaaaaaagc ttgtagcatg gatgttgatt
     4921 ttatttgggt catggatgat gatgcagttc caaataataa tacactaaaa ggtctaatta
     4981 acgatgcaca atttctcgaa gcatctggtg ataattgggg atttttatgc agtaaggttg
     5041 ttagcgaaga taatgaatct attaatgtac catctatatc aaggaaaaga aatcgttcag
     5101 gttatttatc atggagtgaa aaggctgaaa atggtctaat aggtgttgat acagcaacat
     5161 ttgtatcagt acttatgaaa aaagaaatac cttttttggt tgggcttccc gttaaagaga
     5221 tgtttatttg gggagatgat actgaataca cttggcgaat tagcacgcta aaaaattgtt
     5281 atttatctgc aaatagtact atttatcata agagaatcag tgctagtgct ttaagtgttg
     5341 taacggaaaa agaaaaaaca agacttaatt ggtatattta ttattacagg aataatttat
     5401 atgcgcggag aaaacatggt tctaaaaaag atatgcttgt ttttcttatt tcaactatta
     5461 aagacataaa ggatattttt tgtaaatcca aaaataataa attactacgt ctaaagtgtc
     5521 tattgattgg tatttttaaa ggggtggtgt ttaatcccca aattaagaac gtttaaacac
     5581 attaaaagac taaagttata tggtgaatat attgtgactt ctagtaacaa aatactttca
     5641 ttattatttg gggttatcct ttttctgcct ctatttcctg ttcagctaaa tgaaacacaa
     5701 tatcatgtga ctttagccat tttattaggt cttatcttaa tctttcttat gtcgtggaaa
     5761 caattaggag gtttcaaagg cgggagccca gtgctttatt attatctctg cttgcaatta
     5821 ttattgattc ttagcggcgt aatgggggtt gatacagtta aagaatacca agatttaatt
     5881 agcttatttc gtcctatggt tttattgtca tcattttatt tactttattg tctgacatct
     5941 agaaatagag atttctttag gacacttatt aagccaatga aatttatatg tatggctatg
     6001 tttatatggg ctgtttacga gagcattata caaaataaca tatttcaaag tattaattat
     6061 cttttataca aaatggagcg taaagatgac attaaaaatg ttgcagtaac attctttttt
     6121 ttaccttatt attctgcttt tgtatctgtc tttatgatag cattttttta tacatgtcgt
     6181 tacacttata aaataagagg aacatataaa tatatattca tgtctttttg ttgtattgtt
     6241 cttacacaat cgaaaacggg cgttgtagct gcgttgtttt taattgtttg ttgcgagtta
     6301 atatcaagtt cacaattaaa aagaatattt tgtgcagtat gtgtgattat tttgtttttt
     6361 atatcgttct atttttacga ttatttatta atgtatctaa atttatattt ccctggaaat
     6421 tttactcgaa ctgtttcaca acttgcggat gatcctagta gtgcatatac attatctgcg
     6481 agaattgagc agtttctgag catctatagt caaacaatgc aacatatacc atttataggt
     6541 tatggattag ggcgtgattt attaatagag tcgtggcctg ccactatcta tgttcgatat
     6601 ggttggattg gtatttgtgt atttttaata ttcaattttt ttctaatatt taaagggatt
     6661 tgttattatt taacgcatac aagcaatgca ttccgatacc aagcgattat tttttcattt
     6721 tggataatta gtacgcttat ttcacaaatg agtgcaatga tgactgaaaa tacaaagaca
     6781 tcattcatat acttggtata tttggtcata tttatgcaac taatctacaa gcctcataaa
     6841 attaataaat caaatctagg ttaatcaaat gggtaaacat gcattactag taaatgggtc
     6901 ataccttcct actgtcggtg gagtagagaa ttcaattcgc agtatagctg cagagcttgt
     6961 taaagaaggt tggaaagtcg atgtggtatg tagtgatgaa ggtaagtaca gtcgttatga
     7021 aaaaacaaat aatgtaagtg tgtatcgtta tgccaaaaaa ggtttttttc ggtcattata
     7081 taattcgttt agagtattaa aagaaataaa caaaaagtat caactaatta tctgtaggaa
     7141 tcacactctt tttataattc ttaaatttgc tggatataaa aacattcatt acataattcc
     7201 tggtgtttac cattatcaaa ataaaagtga aatgagaggc tcattatcaa aaaaaataaa
     7261 atatgctagc aatgttttta tccagattgt ttcttttaat atttccaaat taaagtatgt
     7321 attctcaccc acaatggaag atcaagtctg ttcagtatct atatttaata agtctgttaa
     7381 aaaaatatat ccaggtgtgg acgatacaag attctataag ctagatagaa aattaaaaaa
     7441 taatttaaag ttagctttgg gatttgataa tgattcaaaa attgttcttg gtctcgggag
     7501 atttgtagat gtcaaaaatt tcgagcatct tattaaatgt gttccttatt tgccctgtga
     7561 ctataaagta gtcttggttg gtggggggga taatttaacg gtttataaaa aaataatagg
     7621 tgagcttaat ttaattaacc gtgtttttat tttcgaaagt acattagagc cagaaaaatt
     7681 ttatcaaata gctgatgttt tttgcctgac atcaacatat gagccctttg gccaagtttt
     7741 gatagaagct tcattttgtg gtttgaacat tgtcgcattc gattctgatc tgaaaggtat
     7801 tgatactgct actaattcaa tttttattca tgattgtgat ttttattttc cagtatcttc
     7861 ctttgagcta tctgattttg ctaaagaaat tattaatgca gttacatgca atagaagctc
     7921 attatgccaa aatgagttta ataaacagta taactggaaa gctttaattg agagattatc
     7981 aattgattaa atattcattt atcataccat gttacaatgt atccaaatac atcttaactg
     8041 ctattgagag tattcctgtc agggaagata tagaaattat cattattgat gatggttcaa
     8101 cggatggttt aagtaatgct atttcttcat ataaaggaaa caaaataata tattttcaca
     8161 aagaaaatgg tggtgtttca tctgcaagaa ataagggaat tgatttagcc acaggtaaat
     8221 atttgctttt ttttgatgga gatgattact attccgagga tctcattaat tatcttgata
     8281 aaggttttga taatgctcaa gcaaaaatga ttacttttgg ttataatcac ctcgcgaaca
     8341 atagattaaa atattattca cccggaacga ctggcataaa aagcacgaga gttttaatag
     8401 ggcaaatatt attacgacaa tcatttcagt gtatgtgttc attcgcggtg gaaagtgatc
     8461 ttgttaaaaa taataatctt agatttaatg aagtaactta ttattatgaa gatattgaat
     8521 ttcagcttaa agtaatgtca ttgatgaatt tcgtttttgt aattgataaa ccattatttt
     8581 tttatatatc gagaaagaag tcagcaacca attcatttgt taatgaaaaa catttttctc
     8641 ttttttatgc tatagataga cttgaaaata ttcttgatgc agatatgaat gattccttaa
     8701 catattttag atggtattct cttttttgga tttttagatt ggcatttaaa aatggatgta
     8761 caaagaaaag tattagtatt ctgaaaacat tgaaattaag caattttaaa tacaatctta
     8821 ttccttgctc tcaaggacga tttaaatata atctcatttg gctttttcag aagttcccta
     8881 tcccttttat ttcttttatt ctaaatttac gcgcaattag aaataattaa cgagcatatt
     8941 cataatgaac actcacaaag taattgctgt gatagtgaca tatcgaagaa aaaactttct
     9001 tgataaagta ctatcagccc ttctcaatca aacagttcct ttacataaag taattgttgt
     9061 tgataataat agttcagatg gtacggacga tattgttaag gaatatatta ataaatttcc
     9121 tgaagttatt agttaccata acacccatga aaatttagga ggggcaggag gtttttatac
     9181 aggaatgaag cttatcgaaa aatataacta taactatgat tatgcttggt taatggatga
     9241 tgatctcatt ccgaacaacg attgtttaga aataatgcta acagcgaaca tcaagggtat
     9301 aatacagccg atgcgcttta atcttgatgg ctcatgtgct gaattatctg cgttaaaata
     9361 tgatctcaac tcttttttta gattaaagcc taaaggagat acagttaaag attttgtagc
     9421 aagtaatcca ttactaccgg atttcattaa tattgagacc gttccttttg aagggccatt
     9481 aatacataaa gatattgtaa ctgctgttgg tttgcctgat ccacgatttt ttatttttgg
     9541 tgatgataca gattattcta tacgaacatt aaaattgaat tatcctatta tatgtatgcc
     9601 ccgagctaga gcaagtcgcc tcttaataaa taatcaaaga aacgatttgt tatcttggaa
     9661 aggttttttt atgttgcgca atctttttca cctttatttt aaacatggca atggctatat
     9721 tgcaagaaca aagccttata ttattgctgt gtgttactta ctactatgct tggtaaaagg
     9781 taattttaag caatctttaa ttacagtaga tgctttaaaa agcgcacgat tgttgatgaa
     9841 taatgataaa tataaaccta aagcttaatg aatacgcttt ttgtatatga aaaacatatt
     9901 atatttgtaa tttaatgcag acagtagatt tgataaaaaa catgttcagt ccgcaacccg
     9961 cggtaacccc ctgacaggag taaacaatgt caaagcaaca gatcggcgtc gtcggtatgg
    10021 cagtgatggg gcgcaacctt gcgctcaaca tcgaaagccg tggttatacc gtctctattt
    10081 tcaaccgttc ccgtgagaaa acggaagaag tgattgccga aaatccaggc aagaaactgg
    10141 ttccttacta tacggtgaaa gagtttgttg aatctctgga aacgcctcgt cgcatcctgt
    10201 taatggtgaa agcaggtgca ggcacggatg ctgctattga ttccctcaag ccatacctcg
    10261 ataaaggtga catcatcatt gatggtggta ataccttctt ccaggacacc attcgtcgta
    10321 atcgtgagct ttctgccgaa ggctttaact tcattggtac cggtgtctcc ggtggtgaag
    10381 aaggcgcgct gaaaggtcct tccattatgc ctggtgggca gaaagaagcc tatgaactgg
    10441 ttgcaccgat cctgaccaaa atcgccgcag tggctgaaga cggtgagcca tgcgttacct
    10501 atattggtgc cgatggtgca ggtcactatg tgaagatggt tcacaacggt attgaatacg
    10561 gcgatatgca gctgattgct gaagcctatt ctttgcttaa aggtggcctg aatctttcca
    10621 acgaagaact ggcgcagacc tttaccgagt ggaataacgg tgaactgagc agctacctga
    10681 tcgacatcac caaagatatc ttcaccaaaa aagatgaaga cggtaactac ctggttgatg
    10741 tgattctgga tgaagcggct aacaaaggta ccggtaaatg gaccagccag agcgcgctgg
    10801 atctcggtga accgctgtcg ctgattaccg agtctgtgtt tgcacgttat atctcttctc
    10861 tgaaagatca gcgtgttgcc gcatctaaag ttctctctgg cccgcaagcg cagccagcag
    10921 gcgataaggc tgagtttatc gaaaaagttc gtcgtgcgct gtatctgggc aaaatcgttt
    10981 cttacgctca ggggttctct caactgcgtg cggcgtctga agagtacaac tgggatctga
    11041 actacggcga aatcgcgaag attttccgtg ctggctgcat catccgtgcg cagttcctgc
    11101 agaaaatcac cgatgcatat gccgaaaatc cgcagatcgc taacctgctg ctggccccgt
    11161 acttcaagca aatcgccgat gactaccagc aggcgctgcg cgatgtcgtt gcttatgcag
    11221 tacagaacgg tatcccggtt ccgaccttcg ccgctgcggt tgcctattat gacagctacc
    11281 gtgccgctgt tctgcctgcg aacctgatcc aggcacagcg tgactatttc ggtgcgcata
    11341 cttataagcg cattgataaa gaaggtgtgt tccatactga atggctggat taatctgatt
    11401 taaatcaatt aatcaatgca aggcccggag aaaccctccg ggctttttta ttatacaaag
    11461 cggcaggtta tggccttttt ttataattta tagttaaaaa cgcgatataa tacagcgccg
    11521 cacagcagga tcgctgcctt gacagttcat ctacatcagc gttaaaaatc ccgcagtaga
    11581 tgaagttgtg gtggtggatt aatgaccact ctaaatgttt aaccggaaga agtcagagct
    11641 aatgaaaata acaatttcag gaacaggtta tgttggtctt tcaaatggta ttctgattgc
    11701 gcaaaatcac gaagtggttg cactggatat cgttcaggcc aaagtggaca tgcttaacaa
    11761 gaagcagtca ccgattgttg ataaggagat tgaagagtat ctggcgacta aagatctcaa
    11821 tttccgcgct acgacagata agtatgacgc gtataaaaat gccgattacg ttattattgc
    11881 cacgcctacc gattatgacc cgaaaacaaa ttattttaat acctcaagcg tggaagcggt
    11941 cattcgcgat gtgacagaaa ttaatcccaa cgcggtaatg gtgattaaat ctacaattcc
    12001 ggtaggtttc accaaatcaa ttaaagaacg tctgggtatc gataatctta ttttctctcc
    12061 ggaatttctc cgtgaaggta aagcccttta cgataatctc catccttcac gtattgtcat
    12121 tggtgagcgt tcagaacgcg cagaacgttt tgctgcgttg ttacaggaag gcgcgattaa
    12181 gcaaaatatc ccgaccctgt ttaccgactc cactgaagca gaagcgatta aacttttcgc
    12241 taatacctat ctggcgatgc gcgtagcata ctttaatgaa ctggatagct atgcagaaag
    12301 tttaggtctg aatactcgcc agattatcga aggcgtttgt ctcgatccgc gtattggcaa
    12361 ccattacaac aatccgtcgt ttggatatgg tggttattgt ctgccgaaag ataccaagca
    12421 gttactggcg aactaccagt ctgtgccgaa taacctgatt tcggcaattg tcgatgctaa
    12481 ccgcacacgt aaagacttta ttgccgatgc cattttgtca cgcaaaccgc aagtggtggg
    12541 tatttatcgt ctgattatga agagcggttc agataacttc cgcgcgtctt ccattcaggg
    12601 gattatgaag cgtatcaagg cgaaaggcgt tgaagtgatc atatacgagc cagtgatgaa
    12661 agaagactca ttcttcaact ctcgcctgga acgtgatctc gccaccttca aacaacaagc
    12721 cgacgtcatt atctccaacc gtatggcaga agagcttaag gatgtggcag acaaagtcta
    12781 cacccgcgat ctctttggca gtgactaaca tcttgttatc agggcgattt tcgccctgat
    12841 tctcttatgc tccctttgta ataattcatt atttttatca tttatcctat agcattcatg
    12901 gcgattatcg ctaaactatg gcgacttaaa attatttcgt ccgttagggt aatgatgaga
    12961 gtagaaaata ataatgtttc tgggcaaaac catgacccgg aacagattga tttgattgat
    13021 ttactggtgc agttgtggcg tggcaagatg acaattatca tctctgccat tgtcgctatt
    13081 gccctggcta tagggtatct ggccgttgcc aaagaaaagt ggacttctac agctatcgtt
    13141 actcaacctg atgtggggca aatcgctggt tataacaatg ccataaatgt gatttatggt
    13201 tcggcagcgc cgaaagtgtc tgagatccag tcgatgttaa ttggtcgttt cagcacgaca
    13261 ttttctgcat tagcagaaac gctggataat caggaagaac ctgaaaaact gaccattgag
    13321 cctaccgtta aaaatcagtc attaccttta gcggtttctt atgtgggtga aacacctgaa
    13381 ggtgcgcaga aacagctggc gcagtatatc cagcaagttg acgaccaggt aaatgaagaa
    13441 ctggaacaag acctgaaaga caacatcgcg ctgcaaatga aaaacttgca ggattctctg
    13501 aagacccagg aagtggttgc gcaggagcag aaagatctgc gtatccgtca gattcaggaa
    13561 gcgttgcagt atgcgaatca ggcgcaggtg acaaagccgc agattcagca gacacaggac
    13621 gtaacccagg atactatgtt ccttttgggg agcgaagcgc tggagtcgat gattaagcac
    13681 gaggcgactc gtccgttggt gttctcatca aactactatc agactcgtca aaacctgctg
    13741 gatatcgaca atcttgacgt tgataaactt gatattcatg cttaccgtta tgtgatgaaa
    13801 ccgacgttac ctattcgtcg cgatagcccg aaaaaggcaa ttaccttgat tctggcggtg
    13861 ctgctgggcg gcatggttgg cgcggggatt gtgttggggc gtaatgctct gcgtaattac
    13921 aacgcgaagt aatctttgca gctttaaaga aaacgggcag ggtggtgaca ccttgcccgt
    13981 tttttttgcc ggatgcgaca acaacatcgc atccgcttac cccgcaactc actgatgccg
    14041 tttacgcagg ttctcaatta ctgtcgttaa atccagatcc tgatcctgca acaacaccag
    14101 caggtgatac atcaaatcag atgcctcgtt ggtcagttca aagcggtcat gtactgtcgc
    14161 tgccagtgcg gtttccaccc cttcttcacc cactt
//
