LOCUS       JX112360               15562 bp    DNA     linear   BCT 04-JAN-2017
DEFINITION  Pseudomonas alkylphenolica strain KL28 epm gene cluster, complete
            sequence; and Cro/CI family transcriptional regulator gene, complete
            cds.
ACCESSION   JX112360
VERSION     JX112360.1
KEYWORDS    .
SOURCE      Pseudomonas alkylphenolica
  ORGANISM  Pseudomonas alkylphenolica
            Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
            Pseudomonadaceae; Pseudomonas.
REFERENCE   1  (bases 1 to 15562)
  AUTHORS   Lee,K., Lim,E.J., Kim,K.S., Huang,S.L., Veeranagouda,Y. and
            Rehm,B.H.
  TITLE     An alginate-like exopolysaccharide biosynthesis gene cluster
            involved in biofilm aerial structure formation by Pseudomonas
            alkylphenolia
  JOURNAL   Appl. Microbiol. Biotechnol. 98 (9), 4137-4148 (2014)
   PUBMED   24493568
REFERENCE   2  (bases 1 to 15562)
  AUTHORS   Lee,K., Lim,E.J., Cho,A.R. and Veeranagouda,Y.
  TITLE     Pseudomonas alkylphenolia complete epm gene cluster
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 15562)
  AUTHORS   Lee,K., Lim,E.J., Cho,A.R. and Veeranagouda,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-MAY-2012) Department of Microbiology, Changwon
            National University, Sarim-dong, Changwon-si, Kyongnam 641-773,
            Korea
REFERENCE   4  (bases 1 to 15562)
  AUTHORS   Lee,K., Lim,E.J., Cho,A.R. and Veeranagouda,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2014) Department of Microbiology, Changwon
            National University, Sarim-dong, Changwon-si, Kyongnam 641-773,
            Korea
  REMARK    Amino acid sequence updated by submitter
FEATURES             Location/Qualifiers
     source          1..15562
                     /organism="Pseudomonas alkylphenolica"
                     /mol_type="genomic DNA"
                     /strain="KL28"
                     /isolation_source="soil"
                     /culture_collection="JCM:16553"
                     /culture_collection="KCTC:22206"
                     /type_material="type strain of Pseudomonas alkylphenolica"
                     /db_xref="taxon:237609"
     misc_feature    1..15562
                     /note="epm gene cluster"
     CDS             complement(51..458)
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|HTH_3,DBD-SUPERFAMILY|0043707"
                     /protein_id="AFP55611.1"
                     /translation="MNGIGARLREERERLGLTQRVFGDIGGVEPNAQGKYESGERTPKA
                     DYLAAVATRGVDALYVLSGTRTLVLQGSLSPEEERLLGAFRGLPAADQAAIAQLLGSLA
                     NALPRAYAKVSRRDSSVFGREVGNGEIAAMR"
     repeat_region   882..916
                     /rpt_type=inverted
     gene            1113..2420
                     /gene="epmD"
     CDS             1113..2420
                     /gene="epmD"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding domain dehydrogenase EpmD"
                     /protein_id="AFP55600.1"
                     /translation="MNISIFGLGYVGAVCAGCLSARGHQVLGVDISQAKVDMINQGKSP
                     IVEPGLEQLLLDGVNHGRLRGTTDVQAAVLATEMSLLCVGTPSKKNGDLDLVYMEAVCR
                     EIGMALRDKGSRHTIVVRSTVLPGTAKNVVIPLLEKASGKRAGVDFGVAVNPEFLREST
                     AIQDYDFPAMTVIGELDQQSGDLLESLYKGLDAPVIRKSIEVAEMIKYTCNVWHATKVS
                     FANEIGNIAKASGVDGREVMDVVCQDYKLNLSRYYLRPGFAFGGSCLPKDVRALTYRAG
                     QLDVEHPLLASIMDSNRNQVRNAFELIARQDKRRIGLLGLSFKSGTDDLRESPLVELAE
                     MLIGKGYELSIYDANVEYARVFGANREYIESKIPHVSSLLCSDLSQVINGADVIVLGNN
                     DDSFAQALDVADGKQVIDLVGFMPHGSNSSQQGICW"
     gene            2577..4100
                     /gene="epm8"
     CDS             2577..4100
                     /gene="epm8"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q52463|4.D.1.1.9"
                     /protein_id="AFP55601.1"
                     /translation="MERQQQPPSGARRAAMSLCAWACLFGLIALASEMIAPAYLDPSHH
                     SFIFFIGTLGMWRYGNAIVHYARGMYFLHWKFPRIRKAVERMGDAALPAHMFMVVTSFR
                     IPTHTTFKVYQSVFQEVQRLKVPCTVIASIVEKGDEQFIKQIMRNEVKDRDDIKLVIVR
                     ARGTGKRDGLAHAFRALSRQMPLEDSVVGVVDGDTMMLPGCVERAVKLFAYLPKVGGLT
                     TNEFCEVEGSNLMRQWHSMRFVQRHINMCSMALSHRVLTLTGRLSFFRASVMTNPDFIR
                     DVEADFLEHWRLGRFQFLTGDDKSSWLSLMRAGWDTFYVPDSNTLTVEHPPDNSFWRAT
                     RLLMFRWYGNSLRQNFRATALLGRARLGLFTLYVLLDQRVSMWTCLMGLTASVVAGLVF
                     GIQYLLVYLFWVLLSRSIVTVLFLFAGHPVSPVYPFVLYYNQIVGSVMKIYALFHMDQQ
                     RWTRQKTTLSNGSVDFDAALNRWSSKAMLFSSIAIFFGVISVLLELTQR"
     gene            4116..5279
                     /gene="emp44"
     CDS             4116..5279
                     /gene="emp44"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane PilZ domain protein Epm44"
                     /protein_id="AFP55602.1"
                     /translation="MSSSSTPTVPGKTAANVVHEAIDERQYVRTRINARVTLAAAGQPT
                     VETTLQDISLGGIGLRHDKPLTIGSLYNASIRLRLNQVDLSLDSKVRIVSQRGEEVGAQ
                     FVDLDAQKRDILRYIISAYLSGEIADINGLFNVMQRENYIKERKQQHASARSSSDRLKA
                     VTGTLLYAAAGLAALGFVAYKSYLLFFRIPAVQAQVSANAQLISMPDNGYVKFLLQPGQ
                     TEVKAGQPLANISTQLATSFTSPADMKAVADLSPGDLQTLLGRATIETVINSPCDCEVY
                     FPSKPLDGYGYKQAPLMHLLPRDEGLFVTANVPFDKLADISRVTSVDLKVFGLDQHFSG
                     KVVDSRVDEANKNLALTIAPDTPLPRQAYQKPVAVDLFLGLPFTASR"
     gene            5300..6751
                     /gene="epmK"
     CDS             5300..6751
                     /gene="epmK"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic SEL1 domain protein EpmK"
                     /protein_id="AFP55603.1"
                     /translation="MNAMMPSRLLLAGLCVACASAVSAGQSLEQIRYALYKDPSANVSS
                     DLRLLSDRGDLASKLLLGDVLANTDGASRQEVLGLYKEAFADGRGQIPALASLARLLDR
                     TPSLQKGQRDWVAQALTRYPATLDPRNASTTLEVFLVYPQLVSNDQAQHLLALYQQSCL
                     LNCRPELYRAVLAERQGDRQTAEHWYQQAVRVDVRAIDRYYRFLGEDQDRAFPAFARSL
                     EAQRPTLAVEIVHRIGSLLDSINSVQRADLESDRQERQVRAMGTAQVQPDAAQLAREKA
                     RSDELELARAEALRWRDHAVERGYVPAIASKASYMISDPTAHNAEETQALINQVQAHEP
                     TRAKALQASFYMVNNWLTLDPQKARALIDELIASHYPDAQLLLADYYSKGVEDEPDQDK
                     ALQIFQAQAQAGSTTAWYRMATLHAYGRAICHDPVKAYTYAQVALELGESGARSLIKRL
                     EKTLKKDDIDRALAARADLLKEATL"
     gene            6748..8205
                     /gene="epmE"
     CDS             6748..8205
                     /gene="epmE"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane porin protein EpmE"
                     /protein_id="AFP55604.2"
                     /translation="MKAHHSLFGALLALTPLYPAMAAEEITGPEADSNEPVRVLPSDGS
                     APVITSEFFNKLTVQTGYGPENSEVGRSREMFYSLRYEPSFAWYSPEKRWAKWMVYGRL
                     WLNYDSSQSISTQNENSNSFDREQPEGWYAELRELYVKRNLLGDDPRFSLSVGRQRYFD
                     DYGIWWDDSLESLRFNYNDTFSRGFFAVGQKFYNYNSDVNSLADSEERTTYLMGEYAYR
                     WSERNWAGVRLMHENDHSGHDPEDRYDFKGMRYGLFFKADDLDVSPLFSDYHLELAGLD
                     GKVDNTDGNGTTSTGNNASGWAALGEIGKRFHDLPWTPRVALRGGLTDKPSNENDGFRL
                     NAIQSDRITRQGSYSTRLTSSFVSLDLRNLSYYGISLETNPTPRSALDLRVTDLKLRDA
                     DGTLPVRIDGDQRSERNRAIAQGNHGGGKDVGQMYDLNYYWKMFPVAIDGKHLDVNALV
                     SASYLNAGNALDSGDDYQLSVGVVISY"
     gene            8220..9647
                     /gene="epmI"
     CDS             8220..9647
                     /gene="epmI"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P39580|2.A.50.2.1"
                     /protein_id="AFP55605.1"
                     /translation="MIFASNVFLFLYLPLFLAVYFLARPQWRSTVIVAASYIFYAWWRP
                     DFLLLFVAITYWNYWFGLRIKARLDAGDKRWALRLLWIGIVGNLSTLGYFKYANFGAEV
                     LAALLAPLGINTWSLETIFLPLGISFYVFHALSYIVDIYRKDATPTNNFIDFAAFVALF
                     PHLVAGPILRYSQLAPQLRQRTHSLELFSLGVCRFMLGFIMKVLIADQLAPINVLFISE
                     GTLQLSDAWFGLVVSTLQLYFDFAGYSHMALGLALMMGFRFPENFNQPYVSQSITEFWA
                     RWHMTLAHFLRDYVYMPLVRKRVAGALPALVYTMLLSGLWHGASFAFIFWGLFFGLGMV
                     IERKYNLATKITTPYHLGRNLRTFVLIILSMPLFFTNDLRHSLDIYQALFGFNGLGSLD
                     LYVLGASKMAMSFAAVALCWLVLAGSNNVRYYAGNKEQYFMRHVGGLKAVLLWAGFALA
                     LSSLAANSFSPFLYFQF"
     gene            9657..10751
                     /gene="epmJ"
     CDS             9657..10751
                     /gene="epmJ"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain protein EpmJ"
                     /protein_id="AFP55606.1"
                     /translation="MYPVMSSASKVNGILFGVVLAGMFVYSLPPVFSFAKSQSDAWNLF
                     VDGKLLRKFEQVYDKRFFLREPSVELWANAQYHLFDEGTKGVVLGKDGWLYTNQEYRVP
                     NNLQANLEGQLAQIAEVEQKLKAHNKRLILLPLPMKVDVYGAHTERAVDPRAISLYDRF
                     IAGLQAQKTEVTPVRSAFLYNSAGPELFLKSDTHWSPEGARLTAYELARQRPDLLGDER
                     YVSHKVAEKTVKGDLMNYIQFNHAQLAPQFGPSQIALYETLKAEQNTDALFADEQQSLL
                     LVGTSYSKIDDWNFTGFLKEALNRDLLSVAVEARGPFEAMNQFLASDDLKNPQIDTVIW
                     EFPLRTLLAHRLGSLSRPGQTQHF"
     gene            10771..11415
                     /gene="epmF"
     CDS             10771..11415
                     /gene="epmF"
                     /codon_start=1
                     /transl_table=11
                     /product="Epm biosynthesis protein EpmF"
                     /protein_id="AFP55607.1"
                     /translation="MNRSLKHLLTASALLLVSTQGVAAEGNADLYDAVAPADSAFVRVL
                     NLSESNIDVTLSGKVNPQRVAAGQLSGYRFTPAGNHKIAVGAKAIEPQLKANAASTVVY
                     DGNTLTLIADKYVNEPKKAQIAFYNLTPAKVALKTVDGKSVVVDTLGKDQTGSRMVNEI
                     KIGFAAYDQQQKIAQFDELFLKKGRSYSYVLLPQGNGYRSMSLANSIDPSE"
     gene            11424..12818
                     /gene="epmX"
     CDS             11424..12818
                     /gene="epmX"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain protein EpmX"
                     /protein_id="AFP55608.1"
                     /translation="MKSLFALGLAVTGALFSVHGAAADSNPATCKGLECLMCPGLSNPQ
                     HYAEGRMKLMREIVPGKDRWLFRSAVDLTNDFGIPAPMRPEFARLMDTFHRQGIQVAMA
                     IQPTRGLMHRDKLYADKLHGFDFARASGNLSSYLQQLRNGGAIVAPMMQVVQNPPEGEF
                     FFRRDHHWTPAGAEATARLVAEEVRRQPFYASLDKKQYRTEPGVMVPKDGTLNLALSYI
                     CGNNYGFQYVRGYQTIPADQGADALFDDAPDPQVILVGDSNAAAREDESKQFNFDGYLK
                     QFLDVDILNYALPGVGQDGSLLEYLLSENYKPKAPPKLIIWELPANYQLESELMYRQLI
                     PAIKGGCPVSTEVMTNRLQRPALKVNERIELLSNAGQARKAVNAGFLDIRISDKNVKDF
                     YVIVYYDNGARDKVWFRREAVVTGGQYYLELSRAKEFAGANLLSVFMEPTKPGSAATQV
                     ETRLCL"
     gene            12809..13924
                     /gene="epmL"
     CDS             12809..13924
                     /gene="epmL"
                     /codon_start=1
                     /transl_table=11
                     /product="PL5"
                     /protein_id="AFP55609.1"
                     /translation="MPVKTWSALALGLCASLQLSHSLAASSIWPPRATAQSAMIADYRT
                     LQCSKGPPAPYTGSLQMQSKYDQRDSSKSTLRSSPDANSAKIGKQVKQFIGGLIYASKR
                     FQRAKTPQQANMALACQDQWLQRWAEAQALLSADASSTGMAARKWAVAAMASTLLMTQA
                     ASDGKLQLSAQQRDWFRQLGERIIAEYQPRRAQGFAWFNNHDYWAAWAVAATGMLIGRD
                     DFIQWADGNLRLGLQQAVKARDGSYAYLPLEVARGKLAATYSQYALVPLVLLAESARVN
                     GLPWSAQDQQTLELLGNFAARSILDPDELPELKGQSQITVAPYKLAWLIPFLQRAPDHR
                     LARQLYDSEDGDVDNYSQIGGPLKPLYPKLP"
     gene            13942..15402
                     /gene="epmG"
     CDS             13942..15402
                     /gene="epmG"
                     /codon_start=1
                     /transl_table=11
                     /product="CASH domain protein EpmG"
                     /protein_id="AFP55610.1"
                     /translation="MAMRPLSLCCLAALLCAGTAQAASQSLPQQQSLSVISNSEHQAQL
                     QQLQQQTAQAVQLQVTERPRPAGRASVSLQPMFSSQSGSWPFEPFVNNGLFRAIAGYQA
                     HHPQAVLIHGGSITLGQLHDALNNPRILKPYKDGYLLSYPLMIGSDGGLVLEGTSLYLY
                     SYSGTALINQGWLGLNQSRLESVAGDKPGSTDRAWRPFVVAWAGSHTQIIGSTLARLGY
                     NANLSRGLSTALSSQQAPGTAQATLIVRNSQFNELSSAVELQHTQAQINDSRFEQSQQY
                     AIDVRDSQVNLVNNQLRGIDNNSGIRLRGQTVARVERNLILGAHKAAIEISEQRGALLL
                     SANLIGDSRGNGLQLRNLAPNAQAPLLIEDNLIGNSQGSAVDASEVSGAILVNNRIGNT
                     PEYAISLRNAAPLAGPLTLSGNTLGKAGKAVVRVEGLQQVVLGRNRFEGKPLLQNLLIG
                     DLLTIQGPLLDATQRQEHVVRVNRNGSQ"
     repeat_region   15451..15479
                     /rpt_type=inverted
ORIGIN
        1 ggatcccgcc accttagcaa aaaaccaggc gctagagcta acggaacacg tcatcgcatc
       61 gctgctattt cgccgttgcc cacttcacgg ccaaaaacac tcgagtcacg cctggaaacc
      121 ttcgcatagg cccttggcag agcattggca agactaccga gcaattgcgc gatggcggcc
      181 tgatcggccg cgggcaagcc gcgaaaagcc cccagcaagc gctcctcctc cgggctcaga
      241 ctgccctgaa ggacaagcgt acgtgtaccg ctcaacacat acagggcatc gacgccacgc
      301 gttgccaccg ccgccaggta atcggccttg ggcgttcgct cgccactctc gtacttgccc
      361 tgggcattgg gctcgacccc accaatgtca ccgaacaccc gctgagtcag ccccagacgc
      421 tccctttctt cccgtagacg ggcaccgatt ccgttcattg cgatccatgt ccttgataca
      481 tcacccagag gggcaactac gtaagaccta caggataggc cagtcgcaca gcactatgca
      541 caggaacacg agtactgcac aagccggcac atgcctcacc tgcctgaggt tgtctgtaca
      601 aggaatttac tcggcgctcg acggccttag aaccgaatat cggcaggtaa actttcatcc
      661 cggcgaaaat ggcactttag tgctactcaa caactatcta aacgccatca ctcaccacaa
      721 ttcaagccaa caaaaaagtt caatgacaac agcaaaaccc agcaacaaac ccgccaaaac
      781 gtcgtcgaaa acgcaataaa actcatttta ttggcgccat aaaaccgcca gagcaacaaa
      841 gcctgttttt tgacacacaa cttattggca actttaagtt attgatttat aagtgttttt
      901 taaacttaaa aagcaaatat caattagttt tgaattaaca acaacagatt gcttgcgtaa
      961 ctaaaaattt cccccttagg atagaaataa gacaacgaac aacacggcca tcgcctgttc
     1021 agtgcgcgat atgtttatgc gtgctcgcct cgtcgacatc accgataaac cgagtaaccg
     1081 ggtcggcatt gccgaacccg gaagagggaa ctatgaacat cagtattttt ggactgggtt
     1141 atgtgggtgc cgtgtgtgcc ggctgtttgt ccgcccgcgg ccaccaggtg ttgggcgtcg
     1201 acatttctca ggccaaggtc gacatgatca accagggcaa atcgccgatt gtcgaacccg
     1261 ggcttgagca actgttgctc gatggtgtga atcatggccg cctgcgcggc accactgatg
     1321 ttcaggccgc cgtgctggcg accgagatgt cgctgctgtg cgtcggcacg ccgagcaaga
     1381 aaaacggtga cctggacctg gtctacatgg aagcggtgtg tcgcgaaatc ggcatggccc
     1441 tgcgcgacaa aggcagccgc cataccatcg tggtgcgcag cacggtactg ccaggcacgg
     1501 cgaaaaatgt ggtcatccca ctgcttgaga aagcttcggg caaacgcgcc ggtgttgatt
     1561 tcggggttgc cgtgaacccc gagttcctgc gcgaaagcac tgcgatccag gactacgact
     1621 tcccggccat gaccgtgatc ggcgagctgg accaacagtc cggcgacctc cttgagtcgc
     1681 tgtacaaggg ccttgatgca ccggtgatcc gcaagtcgat cgaagtcgcc gagatgatca
     1741 agtacacctg caacgtctgg cacgcgacca aggtcagctt tgccaacgaa atcggcaaca
     1801 ttgccaaggc ctccggtgtc gatggccgcg aagtgatgga tgtggtctgc caggactaca
     1861 aactcaatct gtcgcgctac tacctgcgtc caggcttcgc cttcggtggc tcttgcctgc
     1921 ccaaggacgt gcgcgccctc acctaccgcg ccgggcaact ggacgttgag cacccgctgc
     1981 tggcctcgat catggacagc aaccgcaacc aggtgcgcaa tgccttcgag ctgatcgctc
     2041 gccaggacaa acgccgcatc ggcctgctcg gcctgagctt caagtccggt actgacgacc
     2101 tgcgcgaaag tccgctggtg gaactggccg agatgctgat cggcaagggt tacgagctga
     2161 gtatctatga cgccaacgtc gagtacgcac gggttttcgg cgccaaccgc gagtacatcg
     2221 aatcgaagat ccctcacgtc tcgtccctgc tctgcagcga cctcagccag gtcatcaacg
     2281 gggctgacgt gattgtgctg ggcaacaacg acgatagctt cgcccaggcc ctggatgtcg
     2341 ctgatggcaa acaggtcatc gacctggtcg gcttcatgcc ccatggcagc aacagcagcc
     2401 aacagggtat ctgctggtaa cgcctgagac tccgtagaga gcacccggca agcatcccag
     2461 gcctgcgcct gagcgaactg cactcctgcc tctgtctgcc tgccgcactg cggccgggcc
     2521 tggcaagtgc gcgtttcgct caggctcagg cgcctgtaca acggagcaac ggttagatgg
     2581 aacgacagca acaaccgcct tcaggcgcac gacgggcagc catgagctta tgcgcctggg
     2641 cctgcctgtt cggcctgatc gccctggcat cggagatgat cgccccggcg tacctggacc
     2701 cgagccacca cagcttcatc ttcttcatcg gcaccctggg catgtggcgc tatggcaatg
     2761 ccatcgtcca ctacgcgcgc ggtatgtact tcctgcactg gaagttcccg cgcatccgca
     2821 aggccgtcga gcgcatgggc gatgcggcgc tgcccgcgca catgttcatg gtggtgacca
     2881 gttttcgcat ccccacccac accaccttca aggtctacca gtcggtgttc caggaggtgc
     2941 aacggctcaa ggtgccgtgc acggtgattg cctcgatcgt cgaaaaaggc gacgagcagt
     3001 tcatcaagca aatcatgcgc aacgaggtca aggaccgcga cgacatcaaa ctggtgatcg
     3061 ttcgcgcccg tggtaccggc aagcgtgatg gcctggccca tgccttccgc gccctgtcgc
     3121 ggcagatgcc gctggaggat tcggtggtcg gtgtggtcga tggcgacacc atgatgctgc
     3181 cgggctgcgt cgaacgggcg gtcaagctgt tcgcttacct gcccaaggtt ggcggcctga
     3241 ccaccaacga gttctgcgag gtcgaaggca gcaatctgat gcgccagtgg cactcgatgc
     3301 gctttgtcca gcggcacatc aacatgtgct ccatggccct gtcgcaccgg gtgctgaccc
     3361 tgaccggacg gctgtcgttc tttcgcgcca gcgtcatgac caatccggac ttcatccgcg
     3421 acgtcgaagc cgactttctc gaacactggc gcctgggccg cttccagttc ctcaccggtg
     3481 acgacaagtc gtcgtggttg agcctgatgc gtgctggctg ggacaccttc tacgtgcccg
     3541 acagcaatac cctgaccgtc gagcatccac cggacaacag cttctggcgc gccacccgcc
     3601 tgctgatgtt ccgctggtac ggcaactcgc tgcgccagaa ctttcgtgcc accgccctgc
     3661 tcggccgcgc gcgcctgggc ctgttcaccc tgtatgtgct gctcgatcag cgggtgtcga
     3721 tgtggacctg tctgatgggc ctgaccgcct cggtggtcgc aggcctcgtg ttcggcatcc
     3781 agtacctgct ggtgtacctg ttctgggtgc tgctgtcgcg cagcattgtc accgtgctgt
     3841 ttctgttcgc aggccacccg gtgagcccgg tgtacccctt cgttctttat tacaaccaga
     3901 ttgtcggctc ggtgatgaag atctacgcgc tgttccatat ggaccagcag cgctggaccc
     3961 gacagaaaac cactttaagc aacggcagcg tcgacttcga tgccgccctg aaccgctggt
     4021 cctccaaggc gatgctgttt tcttccatcg cgattttctt cggggtcatc tcggttcttc
     4081 tcgaactcac gcaacgttaa ggaaagccgc ctgccatgag tagctcaagt acacctacag
     4141 ttcccggtaa aactgcagcc aatgtcgtcc atgaagcgat cgacgaacgc cagtacgtgc
     4201 gcacccggat caatgcccgc gtcaccctgg cagcggccgg ccagccgact gtggaaacca
     4261 ccttgcagga catttccctg ggcggtatcg gcctgcgtca cgacaaaccg ctgaccatcg
     4321 gcagcctgta caacgcctcg atccgcctgc gcctgaacca ggtcgacctg agcctggaca
     4381 gcaaggtgcg catcgtctcc cagcgcggcg aagaagtcgg cgcccagttc gtcgacctcg
     4441 atgcgcagaa gcgcgacatc ctgcgctaca tcatcagcgc ctacctgtcg ggcgagattg
     4501 ccgacatcaa cggcctgttc aacgtgatgc agcgcgagaa ctacatcaag gaacgcaagc
     4561 aacagcacgc cagcgcgcgc agcagcagcg atcgtctcaa ggccgtgacc ggcactctgc
     4621 tctacgccgc tgccggtctc gccgccctgg gctttgtggc ctacaagagc tacctgctgt
     4681 tcttccgcat tcccgcggtg caggcccagg tctcagccaa cgcccagctc atcagcatgc
     4741 ccgacaacgg ctatgtgaag ttcctcctgc agcccggcca gaccgaggtg aaagccggcc
     4801 agccgctggc caatatctcc acccagctgg ccaccagctt caccagcccg gcggacatga
     4861 aagccgtggc cgacctgtcg ccaggcgatc tgcagacgct gctcggacgc gccaccatcg
     4921 agacggtgat caacagcccg tgcgactgcg aggtttactt cccgagcaag ccgctggatg
     4981 gctacggcta caaacaggcg ccgctgatgc acttgctgcc gcgtgacgaa ggcctgttcg
     5041 ttaccgccaa tgtgccgttc gacaagctgg ccgatatctc ccgggtcacc tcggtggacc
     5101 tgaaggtgtt cggcctggac cagcacttca gcggcaaggt ggtcgactcg cgtgtcgatg
     5161 aagccaacaa gaacctcgcc ctgaccatcg ctccggacac cccgttgcca cgccaggcct
     5221 accagaagcc ggtggcggtg gacctgttcc tgggcttgcc gttcacggca tcgcgttgag
     5281 cgcaaaggaa ggattggcga tgaacgcgat gatgccctcc cgtctgcttc tggcaggcct
     5341 gtgcgtagcc tgtgccagtg ccgtgtcggc cggccagagc ctggagcaga tccgctacgc
     5401 gctgtacaag gaccccagcg ccaatgtcag tagcgacctg cgcctgctgt ccgatcgtgg
     5461 cgacctggcc tccaagctgc ttctcggcga tgtcctggcc aacaccgacg gcgccagccg
     5521 tcaggaagta ctcggcctgt acaaggaagc attcgctgac ggccgtgggc agattccggc
     5581 gctggcctcg ctggcacgtc tgcttgatcg caccccgagc ctgcaaaaag gccagcgtga
     5641 ttgggtagcc caggccttga cgcgctatcc ggcaaccctc gacccgcgca atgccagcac
     5701 caccttggag gtgtttctgg tctacccgca actggtctcc aacgaccagg cgcagcacct
     5761 gctggcgctg taccagcaat cgtgcctgct caactgccgc cccgagctgt accgtgcagt
     5821 gctggctgaa cgccagggcg atcggcagac cgccgagcac tggtaccagc aagcagtgcg
     5881 cgtggatgtg cgggccatcg atcgctacta ccgcttcctc ggtgaagacc aggaccgcgc
     5941 cttcccggcc ttcgcccgta gcctcgaagc ccagcggcca accctggcgg tggagatcgt
     6001 gcaccgcatc ggctcgctgc ttgacagcat caacagcgtg cagcgcgccg acctggaaag
     6061 cgaccgtcag gagcgccagg tacgcgccat gggcactgcc caggtgcaac ctgacgccgc
     6121 gcagctggcc cgggaaaaag cgcgcagcga cgaactggag ctggctcggg ccgaggccct
     6181 gcgctggcgt gaccatgctg tcgagcgcgg ctatgtaccg gcgatagcgt ccaaggccag
     6241 ctacatgatc tccgacccca ccgcgcataa cgcggaagag acccaggccc tgatcaacca
     6301 ggtgcaagcc catgagccga cccgcgccaa ggccctgcaa gcctcgttct atatggtcaa
     6361 caactggtta accctggacc cgcaaaaagc ccgcgcgctg attgatgaac tgatcgccag
     6421 ccactacccc gatgcccagt tgctgctcgc cgactactac agcaagggcg tagaggatga
     6481 gccggaccag gacaaggccc tgcagatttt ccaggcccag gcccaggccg gctcaaccac
     6541 ggcctggtac cgcatggcca ccctgcacgc ctatggccgg gccatctgcc atgacccggt
     6601 caaggcctac acctatgccc aggtggcgct tgaactgggc gaaagtggtg cccgcagcct
     6661 gatcaaacgc ctggaaaaga ccctgaaaaa ggatgacatc gaccgtgccc tggcggcgcg
     6721 cgccgatctg ttgaaggagg caaccctgtg aaagcgcatc actccctgtt cggtgccctg
     6781 ctggccctga caccgctgta cccggccatg gccgccgaag aaatcaccgg ccccgaagca
     6841 gacagcaacg aaccggtgcg ggtgctgccc agcgacggca gtgcgccggt gatcacctcg
     6901 gagttcttca acaaactgac cgtgcagacc ggctacggcc cggaaaactc cgaggtcggc
     6961 cgcagccgcg agatgttcta cagcctgcgc tacgagccat cgtttgcctg gtactcgccg
     7021 gaaaagcgct gggccaagtg gatggtctat ggccgcctgt ggctgaacta tgactccagc
     7081 cagtcgatca gcacgcagaa cgagaacagc aacagcttcg accgcgaaca gcctgaaggc
     7141 tggtacgccg aactgcgcga gctgtacgtc aaacgcaacc tgctcggcga tgacccgcgc
     7201 ttctcgctgt cggtcggccg ccaacgctac ttcgatgact acggcatctg gtgggacgac
     7261 agcctggagt cgctgcgctt caactacaac gacaccttct cccgtggctt ttttgccgtg
     7321 gggcagaagt tctacaacta caacagcgac gtcaacagcc tggccgacag cgaagaacgc
     7381 accacctacc tgatgggtga atacgcctac cgctggagcg agcgcaactg ggccggcgtg
     7441 cgcctgatgc acgagaatga ccacagcggc cacgacccgg aagatcgcta cgacttcaag
     7501 ggcatgcgct acgggctgtt cttcaaggcc gatgacctgg atgtcagccc gctgttcagt
     7561 gactaccacc tggagctggc cggtctcgat ggcaaggtcg acaacaccga tggcaacggc
     7621 accaccagca ccggcaacaa tgccagcggc tgggcagccc tgggcgagat cggcaagcgc
     7681 tttcacgacc tgccctggac gccacgggta gctttgcgtg gcggcctgac cgacaagcca
     7741 agcaacgaaa acgacggctt tcgcctcaat gccatccagt ccgaccgcat cacccgccag
     7801 ggcagctaca gcacgcgcct gaccagttcc ttcgtcagtc tcgacctgcg caacctgagc
     7861 tactacggta tcagcctgga aaccaacccg accccgcgca gtgccctcga cctgcgcgtg
     7921 accgacctga aactgcgcga tgccgacggc accctgccag tgcgcatcga cggtgaccag
     7981 cgcagcgaac gcaaccgcgc cattgcccag ggcaaccacg gtggcggcaa ggatgtcggg
     8041 caaatgtacg acctcaatta ctactggaag atgtttcccg tggccatcga cggcaagcac
     8101 ctggacgtca acgccctggt cagcgccagc tacctcaatg ccggcaacgc cctggacagc
     8161 ggtgatgact accagctgag tgtcggcgtg gtgatcagct actaaggacg gaccctccga
     8221 tgatcttcgc ctccaacgtc tttctgttcc tctacctgcc gttgttcctg gccgtgtact
     8281 tcctcgcccg gccgcagtgg cgctcgaccg tgatcgttgc tgccagctac atcttctatg
     8341 cctggtggcg gccggacttt ttgctgctgt ttgtcgccat cacctactgg aactactggt
     8401 tcggcctgcg catcaaggcg cgcctggatg ccggcgacaa acgctgggcc ctgcgcctgc
     8461 tgtggatcgg tatcgtcggc aacctgtcga ccctgggcta cttcaaatac gccaactttg
     8521 gcgccgaagt gctcgccgcc ctgctcgcgc cgctgggtat caacacctgg agcctggaga
     8581 ccatcttcct gccgctgggc atctccttct atgtgttcca cgccctgagt tacatcgtcg
     8641 acatctaccg caaggacgcc acgccaacca acaacttcat cgacttcgcc gccttcgttg
     8701 ctctgttccc gcacctggtc gccggcccga tcctgcgtta cagccagctg gcgccgcagc
     8761 tgcgccagcg cacccactcc ctggagctgt tttccctggg cgtgtgtcgt ttcatgctcg
     8821 gcttcatcat gaaggtgctg atcgccgacc agctggcacc gatcaacgtg ctgttcatca
     8881 gcgaaggcac cctgcagctc agcgacgcct ggttcggcct ggtggtctcg accctgcagc
     8941 tgtacttcga cttcgccggc tacagccata tggccctggg cctggcgttg atgatgggct
     9001 tccgttttcc ggagaacttc aaccagccct acgtctcgca gagcatcacc gagttctggg
     9061 ctcgctggca catgaccctg gcgcatttcc tgcgcgacta cgtgtacatg ccgctggtgc
     9121 gcaaacgggt ggccggggca ctgccggcac tggtctacac catgctgctg tcggggctgt
     9181 ggcacggcgc aagcttcgcc ttcatcttct ggggtctgtt ctttggcctg gggatggtca
     9241 tcgaacgcaa atacaacctg gcgaccaaga tcaccacgcc ctaccacctg ggacgcaacc
     9301 tgcgcacctt cgtgctgatc atcctgagca tgccgctgtt cttcaccaac gacctgcgtc
     9361 acagcctgga catctaccag gcgctgttcg gcttcaacgg cctgggctcg ctggacctgt
     9421 acgtgctcgg agcctcgaag atggccatgt cgtttgccgc cgtcgccctg tgctggctgg
     9481 tgctggccgg cagcaacaac gtgcgctact acgccggcaa taaagagcag tacttcatgc
     9541 gtcatgtcgg tggcctcaag gccgtgctgc tgtgggccgg tttcgccctg gcgctgagca
     9601 gcctggcagc gaactccttt tcaccctttc tgtacttcca gttctaggag acggccatgt
     9661 acccggtcat gagttcggcg agcaaggtca acggcatcct ctttggcgta gtgctggcag
     9721 ggatgttcgt ctattccctg ccgccagtgt tcagcttcgc caaaagccag agcgatgcct
     9781 ggaacctgtt tgtcgatggc aagttgctgc gcaaattcga gcaggtctac gacaagcgct
     9841 tcttcctgcg cgaaccctcg gttgaactgt gggccaacgc ccagtaccac ttgttcgacg
     9901 aaggcaccaa aggcgtggtg ctgggcaagg acggctggct gtacaccaac caggagtacc
     9961 gcgtaccgaa caacctgcaa gccaacctgg aaggccagct ggcgcaaatc gccgaggtcg
    10021 agcaaaagct caaggcgcat aacaaacgcc tgatcctgct gccgctgccg atgaaggtgg
    10081 atgtctatgg cgcccacacc gagcgtgcgg tcgatccgcg ggcgatcagc ctgtacgacc
    10141 gcttcattgc cggcctgcag gcgcagaaaa ccgaggtcac gcccgtgcgt tcggccttcc
    10201 tgtacaacag cgccggcccg gaactgttcc ttaaatccga cacccactgg agcccggaag
    10261 gcgcgcggct gacggcctat gaactggccc gtcagcgccc tgacctgctc ggcgatgagc
    10321 gctacgtgtc acacaaggtc gcggaaaaaa ccgtcaaggg cgacctgatg aactacatcc
    10381 agttcaatca cgcccaactg gccccgcagt tcggccccag ccagatcgcc ctgtacgaaa
    10441 ccctcaaggc cgaacagaac actgacgcgt tgttcgccga cgaacagcag agcctgctgc
    10501 tggtcggcac cagctacagc aagatcgacg actggaactt caccggtttt ctcaaagagg
    10561 cgctcaaccg cgacctgctc agtgtcgcgg tggaagcccg cggcccgttc gaggcgatga
    10621 accagttcct cgccagcgac gacctcaaga acccgcagat cgacacggtg atctgggagt
    10681 tcccgctgcg caccctgctc gcccaccgcc tgggctctct gagccgtccc ggccagaccc
    10741 agcatttctg acactttcaa ggagatcgac atgaaccgtt cgctcaagca cctgctcacc
    10801 gccagcgcct tgttgctggt cagcacccaa ggcgtggccg ccgaaggcaa cgccgacctg
    10861 tacgacgccg tggccccggc cgactccgcc tttgtgcggg tgctcaacct gtccgagagc
    10921 aacatcgacg tgactctgag cggcaaggtc aacccgcagc gggtcgccgc cggacaactg
    10981 agtggctacc gtttcacccc ggccggtaac cacaagatcg ccgtcggtgc caaggccatc
    11041 gagccacagc tcaaggccaa tgccgcgagc accgtggtct atgacggcaa cacgctgacg
    11101 ctgattgccg acaagtacgt caacgagccg aaaaaagcgc agatcgcctt ctacaacctg
    11161 accccggcca aggtcgcgct gaaaaccgtc gatggcaaga gtgtggtggt cgacaccctg
    11221 ggcaaggacc agaccggtag ccgcatggtc aacgaaatca agatcggttt tgccgcctat
    11281 gaccagcagc agaaaatcgc ccagttcgac gaactgttcc tcaagaaagg ccgttcctat
    11341 agctacgtac tgctgcctca gggcaacggc tatcgcagca tgagcctggc caacagcatc
    11401 gacccgagcg agtgagcggc gccatgaaaa gcctattcgc ccttggcctg gccgttaccg
    11461 gcgcgctgtt cagcgtgcac ggcgcggccg ccgacagcaa cccggccacc tgcaaaggcc
    11521 tggagtgcct gatgtgcccc ggcttgagca atccccagca ctatgccgaa gggcgcatga
    11581 agctgatgcg cgaaatcgtg ccgggcaagg atcgctggct gtttcgctcg gcggtcgacc
    11641 tgaccaacga cttcggcatc ccggcgccga tgcgcccgga gttcgcacgc ctgatggaca
    11701 ccttccatcg tcagggtatc caggtggcca tggccatcca gccaacccgc ggcctgatgc
    11761 accgcgacaa gctgtacgcc gacaaactgc atggtttcga cttcgcccgc gccagcggca
    11821 acctgagcag ttatctgcaa caactgcgca acggcggtgc catcgtcgca ccgatgatgc
    11881 aggtggtgca gaacccgccc gagggcgagt tcttcttccg ccgcgatcac cactggacgc
    11941 cggcgggcgc tgaagccacc gcccgcctgg tggccgagga agtacgccgt cagccgttct
    12001 atgcctcatt ggacaagaag cagtaccgca ccgagcctgg ggtgatggtg cccaaagacg
    12061 gcaccctgaa cctggcgctg agctatatct gcggcaacaa ctacggtttc cagtacgtgc
    12121 gtggctacca gaccatcccc gcggaccagg gcgccgatgc cttgttcgac gacgcgccgg
    12181 atccgcaggt gatcctggtc ggcgacagta acgccgccgc ccgggaagac gagagcaagc
    12241 agttcaactt cgacggctac ctcaagcagt tcctcgacgt cgacattctc aactacgccc
    12301 tgcccggcgt cggccaggac ggctcgttac tcgaatacct gctgtcggag aactacaagc
    12361 ccaaggcgcc gcccaagctg atcatctggg agttgccggc caactaccag ctggaatccg
    12421 agctgatgta ccgccagctg attccagcga tcaagggcgg ctgcccggtg tccaccgagg
    12481 tcatgaccaa ccgcctgcag cgtccggcgc tgaaggtcaa cgagcgtatc gaactgctca
    12541 gtaacgcagg ccaagcgcgc aaggcggtga acgctggctt cctcgacatc cgcatcagcg
    12601 acaagaacgt caaggatttc tacgtgatcg tctattacga caacggcgcg cgcgacaagg
    12661 tctggttccg ccgcgaggcg gtggtcaccg gtggtcagta ctacctggaa ctgagccgcg
    12721 ccaaggagtt tgccggggcc aatctgctct cggtgttcat ggagccgacc aagcccggca
    12781 gcgctgctac ccaggtggaa acccgactat gcctgtgaag acctggagcg cactggccct
    12841 gggtctgtgc gccagcctgc aactgagcca cagcctggcg gccagctcga tctggccgcc
    12901 acgtgccact gcgcagtcgg cgatgatcgc cgactaccgc accctgcagt gcagcaaggg
    12961 gccacccgcg ccctataccg gcagcttgca gatgcagagc aagtacgacc agcgcgattc
    13021 cagcaagtcg accctgcgca gcagtcccga tgccaacagc gcaaagatcg gtaagcaggt
    13081 caaacagttt atcggcggct tgatctatgc cagcaagcgt ttccagcggg cgaaaacgcc
    13141 ccagcaagcg aacatggccc tggcctgcca ggaccagtgg ctgcaacgct gggccgaagc
    13201 ccaggccctg ctcagcgccg acgccagcag caccggcatg gccgcgcgca agtgggcggt
    13261 ggcggcgatg gcctcgacct tgctgatgac ccaggccgcc agcgacggca aattgcagct
    13321 gtcggcgcag cagcgcgact ggttcaggca gttgggcgaa cgcatcattg ccgagtacca
    13381 gccgcgccgg gcgcaaggtt ttgcctggtt caacaaccat gactactggg ccgcttgggc
    13441 ggtagccgca accggcatgt tgatcgggcg tgacgacttc atccagtggg ccgacggcaa
    13501 cctgcgcctt ggcctgcaac aggcggtcaa ggcccgcgat ggcagctatg cctacctgcc
    13561 gctggaagtc gcccgcggca aactggccgc cacctatagc caatacgccc tggtgccgct
    13621 ggtgttgctg gccgagtcgg cgcgggtcaa tggcttgccc tggagcgcgc aagaccagca
    13681 gaccctggaa ctgctgggca atttcgccgc acgcagcatc ctcgatcccg acgaactgcc
    13741 cgagctcaag gggcaatcgc agatcaccgt agcgccttac aagctggcct ggctgatccc
    13801 cttcctgcag cgcgcaccag accatcgcct ggctcgccag ctgtacgaca gcgaagacgg
    13861 cgacgtcgac aactacagcc agattggcgg cccgctcaag ccgctgtacc ccaaactgcc
    13921 gtaacccaag gacgaatcgc catggccatg cgccctcttt ccctttgctg cctcgccgcc
    13981 ctgctctgcg caggcacggc ccaggcggca agccaaagcc tgccacagca gcagtcgctg
    14041 agcgtgatca gcaacagcga acatcaggca cagttgcaac agctgcaaca acagactgcc
    14101 caggccgtgc agttgcaggt cacggaacgc ccgcgtccgg ccgggcgtgc cagtgtcagc
    14161 ctgcaaccga tgttttcctc gcagagcggc agctggccgt tcgaaccgtt cgtcaataat
    14221 ggcctgttcc gcgccattgc cggctaccag gcacatcacc cgcaggcggt actgattcat
    14281 ggcggcagca tcactctggg gcaactgcac gacgcgctga acaacccgcg gatcctcaag
    14341 ccctacaagg acggctacct gctcagctat ccactgatga tcggcagtga tggcggtctg
    14401 gtgctggaag gcaccagcct gtacctgtac agctactcgg gcacggcgct gatcaaccag
    14461 ggctggctgg gcctgaacca gtcacgcctg gaaagtgtcg ccggcgacaa accgggcagc
    14521 accgaccgtg cctggcgccc gttcgtggtg gcctgggccg gcagccatac ccagatcatc
    14581 ggctcgaccc ttgcacgcct gggttacaac gccaacctgt cccgtggcct gagcaccgcc
    14641 ctgagcagcc agcaagcgcc gggcacggcc caggctacgc tgatcgtgcg taacagccag
    14701 ttcaacgagc tgtccagcgc cgtcgagttg caacacacgc aagcgcagat caacgacagt
    14761 cgtttcgagc agtcacagca gtacgccatc gacgtacgcg acagccaggt caacctggtc
    14821 aacaaccaac tgcgcggcat cgacaacaac agtggcatcc gcctgcgcgg acagacggtg
    14881 gcacgggtcg agcgcaacct gatcctcggc gcgcacaagg ccgccatcga aatcagcgag
    14941 cagcgcggcg ccctgctgct cagcgccaac ctgatcggcg acagccgcgg caacggcctg
    15001 caactgcgca acctggcacc caacgcccag gcgcccctgc tgatcgagga caacctgatc
    15061 ggcaacagcc agggcagcgc cgtggatgcc agcgaagtga gcggcgcaat cctggtcaat
    15121 aaccgtatcg gcaacacccc ggaatatgcc atcagcctgc gtaatgccgc gccattggca
    15181 ggtccactga ccctcagtgg caacaccctg ggcaaggcag gcaaggcagt ggtacgggtc
    15241 gaaggactgc agcaggtggt gctcgggcgc aatcgcttcg aaggcaaacc gctgttgcag
    15301 aacctgttga tcggtgacct gctgaccatt caggggccgc tgctcgacgc cacccagcgc
    15361 caggagcacg tggtgcgcgt caaccggaac ggtagtcaat agtccgctgc atgcgcacga
    15421 agcattcatc ctcgcccatt gcttgaaacc cccggcgctg gtacagggcc tgcgccgggt
    15481 tgcttttgaa cactgtcaga cgtatcagcg gtaaacgccg ctcggcagcc cagccggcca
    15541 ggtgctccaa tacctgggta cc
//
