LOCUS       GU299798               11203 bp    DNA     linear   BCT 20-JUL-2011
DEFINITION  Escherichia coli strain G1189 O antigen gene cluster, complete
            sequence.
ACCESSION   GU299798
VERSION     GU299798.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11203)
  AUTHORS   Perepelov,A.V., Li,D., Liu,B., Senchenkova,S.N., Guo,D.,
            Shashkov,A.S., Feng,L., Knirel,Y.A. and Wang,L.
  TITLE     Structural and genetic characterization of the closely related
            O-antigens of Escherichia coli O85 and Salmonella enterica O17
  JOURNAL   Innate Immun 17 (2), 164-173 (2011)
   PUBMED   20501518
REFERENCE   2  (bases 1 to 11203)
  AUTHORS   Perepelov,A.V., Li,D., Liu,B., Senchenkova,S.N., Guo,D.,
            Shashkov,A.S., Feng,L., Knirel,Y.A. and Wang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2009) TEDA School of Biological Sciences and
            Biotechnology, Nankai University, 23 Hongda Street, Tianjin 300457,
            P.R. China
FEATURES             Location/Qualifiers
     source          1..11203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="G1189"
                     /db_xref="taxon:562"
                     /note="serogroup: O85"
     misc_feature    1..11203
                     /note="O antigen gene cluster"
     gene            1103..2239
                     /gene="mnaA"
     CDS             1103..2239
                     /gene="mnaA"
                     /note="UDP-N-acetyl glucosamine-2-epimerase"
                     /codon_start=1
                     /transl_table=11
                     /product="MnaA"
                     /protein_id="ADC54973.1"
                     /translation="MNRKILVVFGTRPEAIKMIPVVQELKKEKDFIVKVCITAQHRQML
                     DQVLNIFNIQPDYDLNVMESGQTLDDITLKVLKGVSSVLKDFSPDLVLVHGDTTTTFAS
                     SLACYYQKIDVGHVEAGLRTGDIYSPWPEEGNRRLTAVLAKYHFSPTKDASFNLKREGY
                     NPDCIFVTGNTVIDSLMRAKDIIKDNESLQKEFSNKFGFIGNDKKLILVTGHRRENFGL
                     GFENVCKSLAEVAYKYTNVNIVYPVHLNPNVQEPVKRILGDIPNIFLIEPQEYLPFVYL
                     MNRAYLILTDSGGIQEEAPSLGVPVLVTREVTERPEAVKAGTVKLVGTTPIKIVSAIDE
                     LLLDDNKYKKMSRAHNPYGDGLASVRICDILTKVLTAI"
     gene            2266..3384
                     /gene="wzy"
     CDS             2266..3384
                     /gene="wzy"
                     /note="O antigen polymerase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ADC54974.1"
                     /translation="MVVLYFSIILLSSLFFTTSFSFYGLSFYGWLAAFILLLPFVRRVK
                     KGKVFIIILPAISIMSFFLSKGHELDGLINNVKLNGIILITVGAMYLIITSDIMKKIPP
                     KIMSNIIAVVMITHVLIAFFQFVYWYAFGGDLDVSSIFGGSGHRSFYYGIYRITGVFDE
                     PAIYSTFIMSFCFLRFYLMGRNDWVNYVGVVSIILSLSFAGIVIVFLYLLVSETWDKKI
                     IFISIMIFVVCLFVASFYPEIYGSVLTRLETLQAGNDGSSNLKFQYVNFWLSDEQRILF
                     GNGMVGYYDGKPKFFESAFDLTFIFTTITTYGVVLSIPLFLVLYYYLFKGQKRKNQLLL
                     LIISLKMTSIIFPFFWVFSGIIIAYEKNKYSS"
     gene            3359..4411
                     /gene="wfbQ"
     CDS             3359..4411
                     /gene="wfbQ"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="GT4"
                     /protein_id="ADC54975.1"
                     /translation="MKKINIVVDDLSTKGGVESVVTSLANGFIKKGVKVNFICVKKCKP
                     NFEIHKDINIIYLLDKWSKLRKYRVLIRFFREKLNTEDILYTNSVINTLLAILFAPKKT
                     AKYACDHNQYNAVNRLWAFFRFLLYKRLEGVIVLTQHDLIKYRKINSNCKLFDNPVSSS
                     FFYTDKKSSATVKKYILNIGRLEKQKGQDLLLAAWNLVNKKGYELWICGDGSQKQTLEQ
                     QTKLYGLTTSVKFLGNRDDIDILLRGAYCNVLSSRFEGKPVSLIESKVVGCPNISFNCK
                     TGPAEIINHKYDGLLIDAGNIEALAKGIQLLIDNPIIRDEYAKNACMNNERYMLENICE
                     EYLSFFNIKA"
     gene            4420..5640
                     /gene="wzx"
     CDS             4420..5640
                     /gene="wzx"
                     /note="O antigen flippase"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ADC54976.1"
                     /translation="MNLKRNGILFYALTILNGLVPLITISFMSRILTSDQLGNYLLSFT
                     IMNVLGMVVDFGISISGVRTLKKNNLNLTLDEFICSSFLLKFFVFFLVSICYLVFFTYT
                     ATEEEMFFIVTLLGVFITGMDSLWILQVSGDLYKILTKNIIANILYVMMVMLVAYITKD
                     YLSTLILFVIYKLYILLLTINEVRKLYVLSRNNFSIINFISVFKSTAGIAMFRFFALSY
                     TSANGILLNYLTNKYQVALYLAFEKLNKAILFIATPLTQALLPLFVGGKNNNKLIKYII
                     SIIIISVFILVIGNLLSKEIVGLFIGTRYLNDDTSEFFFYMTIVSPLILLSNAIGMLYF
                     IPCKLDRFLNCIICSAAFINIICVLYFVHSDVNGALQMARVVAFSELWVTLFMAVVAII
                     HGRKYGR"
     gene            5630..6796
                     /gene="wfbR"
     CDS             5630..6796
                     /gene="wfbR"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="GT4"
                     /protein_id="ADC54977.1"
                     /translation="MEGKILYICSDFYPMSTGYSNAFSNFLDAIGNYQVDILTTTKDAS
                     LKKSAINRAIFKSKVIYGCGALDYIVNSLILYGNYRRLIRKYNYDLIFIETFDDALFLS
                     LIPESEYKTIIVRIHSTSETEYTYFFPGKKYKLNRFLQRKIIAPKIKNICSTSAFHNGF
                     IKKNMFYDNLYDICQKNFFVLPNTMPDVKNFFCKDKNNEEKVKLFSLGRLNREGMCQKG
                     QHDLIMAISLLGKSFSDIASCTIVGDGEYYEYLESLIYKLGLSECIKLIRKMSHDEIIS
                     HLSLSDIVVLPSRFEGMPMFALEALYTQNLCLFSNTGGLSDMIVNEDYSYPPQDIDALA
                     KAIDRAIHLNKSDLYNEKMNSYEKALSFSYENVKNKFDKIFKMVVSGI"
     gene            6793..7908
                     /gene="wfbS"
     CDS             6793..7908
                     /gene="wfbS"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="WfbS"
                     /protein_id="ADC54978.1"
                     /translation="MIIFLRSYSISKDSRLLKYISICKENNIRYKIIGWDKDHNEQYRD
                     DAYYFKGGFKLGKKWLNVMAIFKWWIFIFYLIIFRIKNNITAIHSVDLDCGIVGLIISK
                     LLRAKHIYDIYDVYSANRNMRGLIKALIDSLERFVCRKSTYFIMPESFRFEQLGIDQDN
                     INYNNFIEIENVPQIDRNIELRYISPGQYDFYLAYAGSLEPRHRGLENLLKAVSICPNV
                     FLDIAGVGPLTALCKKYSQQFNNICFHGGLTPQQVYELEANAQIIIGMYYKTRDNHLFA
                     SPNKYYEHLALGKVMLTTSGTPPGMKVEKYKTGFAIGESLQDIIDFLQSLNGKEDIIDN
                     FSINAYRIWVDKYSDYNERYLSKQYMKMVGK"
     gene            7908..9008
                     /gene="glf"
     CDS             7908..9008
                     /gene="glf"
                     /note="UDP-galactopyranose mutase"
                     /codon_start=1
                     /transl_table=11
                     /product="Glf"
                     /protein_id="ADC54979.1"
                     /translation="MYDYLIVGSGLFGSVFAYEMKKKGYSIKVIEKRAHIGGNIYCEEI
                     EGINVHKYGAHIFHTNEKEIWEYVNSFVEFNRFTNSPVALSKGEIYNLPFNMNTFNKMW
                     GVTSPSAAKNKIEQQRKEIGNKEPANLEEQAISLVGRDIFETLIKEYTEKQWGRECKNL
                     PAFIIKRLPVRFTYDNNYFNDRYQGIPIGGYNRIIEKMLEGVDVQLNTDFFDNKDYWIS
                     SARKIVYTGPIDRFFNYEFGELDYRTLSSEHEIKNESNFQGNAVVNYIDSSYEFTRIIE
                     HKHFEFGEQDKTVITKEFPKEYVNGDEPYYPVNDSENNRKYAKYKLKTSQFDNVIFGGR
                     LAEYKYYDMHQVIASALKAVSKEVSQ"
     gene            9005..9919
                     /gene="wfbU"
     CDS             9005..9919
                     /gene="wfbU"
                     /note="glycosyltransferase"
                     /codon_start=1
                     /transl_table=11
                     /product="GT2_Glycos_transf_2|GT2"
                     /protein_id="ADC54980.1"
                     /translation="MKMPRVICIIVTFNREHLLQQTLDAVLNQTFPLQNIIVIDNNSFD
                     NTRSLVSKLSENNPKIEYHNTGANIGGAGGFHRGFAVASKYKYDYLWLMDDDLKPEIEC
                     LYNLISSATEHGIYQPIRINVDGSCAELSPVVYDLKNPFFMNPKRLSVINLYKRMNNAV
                     YSPFDIHGVPFEGPLISRDVIQCIGYPNPNFFIFYDDLDYSLRARKAGFEIKCIPSARA
                     VRLLKNNQRSDLTSWKGYFMFRNLFYIHRTYGENIFVRLKPFLLVLGYLVFLIFRFKFN
                     QVPTILSAFKDSRKLDNSELHKP"
ORIGIN
        1 attgtggctg cagggatcaa agaaatcctc ctggtaactc acgcgtccaa gaacgcggtc
       61 gaaaaccact tcgacacctc ttatgaatta gaatctcttc ttgagcagcg cgtgaagcgt
      121 caactgcttg cggaagtgca gtccatctgt ccgccgggcg tgaccattat gaacgtgcgt
      181 cagggcgaac ctttaggttt aggccactcc attttgtgtg cacgacccgc cattggtgac
      241 aacccatttg tcgtggtgct gccagacgtt gtgatcgacg acgcgagtgc cgatccgctg
      301 cgttacaacc ttgctgccat gattgcgcgc ttcaacgaaa cgggccgcag tcaggtgctg
      361 gcaaaacgta tgccgggtga cctctctgaa tactccgtta ttcagaccaa agaaccactg
      421 gatcgtgaag gtaaagtcag tcgcatcgtc gaatttatcg aaaaaccgga tcagccgcag
      481 acgctggact cagatatcat ggccgttggt cgctatgtgc tttctgccga tatttggccg
      541 gaacttgaac gcactcagcc tggtgcatgg gggcgtattc agctgactga tgccattgcc
      601 gaactggcga aaaaacagtc cgttgatgcc atgctgatga ccggcgacag ctacgactgc
      661 ggtaaaaaaa tgggctatat gcaggcgttt gtgaagtatg gactacgcaa cctgaaagaa
      721 ggggcgaagt tccgcaaagg tattgagacg ctgttaagcg aataatgaaa atctgaccga
      781 atgtaacggt tgataagaaa attataacgg cagtgaagat tcgtggcgaa agtaatttgt
      841 tgcgaatatt cctgccgttg ttttatataa acaatcagaa taacaaagag ttagcaatag
      901 gattttcgtc aaagttttca ggattttcct tgtttccaga gcggattggt aagacaatta
      961 gcgtttgaat ttttcggatt tagcgcgagt ggttaacgct cgtcacatcg taggcatgca
     1021 tgcagtgctc tggtagctga aaagccaggg gcggtagcgt gtcgtttttt gtaaacataa
     1081 tttttacatt gctgagagat atatgaaccg aaagatactg gtagtatttg gaacgcggcc
     1141 agaagctatt aaaatgattc ctgttgttca ggaattgaaa aaggaaaaag actttattgt
     1201 gaaagtctgt atcactgctc aacatagaca aatgcttgat caggtattga acatttttaa
     1261 tattcagcct gactatgact taaatgtgat ggaaagtggg caaacattag acgatataac
     1321 attaaaggtt ctcaaagggg tatcgtcagt attgaaagat ttctcgccag acttagtatt
     1381 agtccatgga gataccacaa ctacttttgc tagtagcttg gcgtgttatt atcaaaaaat
     1441 tgatgtcggg catgttgaag ctggtttacg tactggcgat atttattcac catggccaga
     1501 agaaggtaat agaaggttaa ctgccgtatt agctaaatat cacttctcgc caacaaaaga
     1561 tgcgtcgttt aatttaaaac gagaggggta caatcccgat tgcatatttg taacaggaaa
     1621 tactgttatt gattctttaa tgagagccaa ggatataatc aaagataatg aaagtttgca
     1681 gaaggaattt tctaataagt ttggttttat tggtaatgac aaaaaactga tattggtaac
     1741 aggtcataga agagaaaatt tcggattggg atttgaaaat gtttgtaaat cgttagccga
     1801 ggtggcatat aaatatacta acgtaaacat tgtataccct gttcatctta atccaaatgt
     1861 tcaggaacct gtaaaaagga tattgggtga tatacctaat attttcttga tagaaccgca
     1921 agaatatctt ccatttgttt atctaatgaa tagggcttac ctgattctta cagactcagg
     1981 aggaattcag gaagaagcac cgtcgctagg cgtaccagta ttggttacaa gggaggtaac
     2041 agaaaggcca gaggctgtta aagcagggac ggttaaatta gtcggtacaa cccccataaa
     2101 aatagtatct gctattgatg aattactcct tgatgataat aaatataaaa aaatgtcacg
     2161 agcgcataat ccatacggag atggtttagc atctgttaga atatgtgata tattaactaa
     2221 agtattaact gcaatataaa gaatgttaac taagtgactt atattatggt ggttctttat
     2281 tttagtatta tattgctgtc atctcttttt tttacaacaa gttttagctt ttatggattg
     2341 tcattttatg gctggctggc agcatttatt cttttacttc catttgttag gagagtaaag
     2401 aaagggaaag tatttataat aatattacct gcaatatcaa taatgtcttt tttcttatca
     2461 aaaggccatg agcttgatgg attaataaac aatgtgaaat taaatggtat aattttgata
     2521 acagtaggcg caatgtatct tattattacc tctgatatca tgaaaaaaat accacctaaa
     2581 ataatgagta acattattgc tgtagtaatg attactcatg ttttgattgc atttttccaa
     2641 tttgtttatt ggtatgcttt cggaggagat ctcgatgtca gttcgatttt tggaggaagt
     2701 ggtcatcgct cattttacta tggcatttat agaataactg gagtgtttga cgagccagct
     2761 atttattcaa catttatcat gtctttctgt tttttacgtt tttatttaat ggggagaaat
     2821 gactgggtga attatgttgg tgttgtcagt ataatattat cattgtcgtt tgcgggtatt
     2881 gttatagtat ttctttattt gcttgtttct gaaacttggg ataaaaaaat tatttttata
     2941 agtataatga tttttgtggt ttgcttattt gtagcttctt tttatcctga aatatatggt
     3001 tcagtattaa ctcgtttaga aaccttacaa gctgggaatg atggaagcag taacttaaag
     3061 tttcagtacg ttaacttttg gttgagtgat gaacaacgta ttttatttgg caatggaatg
     3121 gttgggtatt atgatggaaa gcctaagttt tttgagtctg catttgattt gacttttatt
     3181 tttacaacca ttacaactta tggtgttgtg ttatcaattc ctctattttt agttttatat
     3241 tattacttat ttaaaggaca gaagagaaaa aatcaattat tgcttctaat tataagtttg
     3301 aaaatgacat ctataatatt tccatttttt tgggtcttca gtggtatcat aatagcatat
     3361 gaaaaaaata aatatagtag ttgatgatct atctactaaa gggggagtgg aatcagtagt
     3421 aacttcacta gccaatgggt tcattaaaaa aggagtaaaa gtaaacttta tatgtgtaaa
     3481 aaaatgtaaa cctaattttg aaattcataa agatataaat ataatatatt tgcttgataa
     3541 atggagtaaa ttacggaagt atagagtgtt aataagattt ttcagggaaa aattaaatac
     3601 cgaggacata ttatatacta actctgtaat taatacatta ctggctatcc tttttgctcc
     3661 gaaaaaaaca gctaaatatg catgtgatca taatcagtat aatgcagtaa atagactatg
     3721 ggcttttttc cgatttttgc tatataaaag gttggaagga gtaattgttc taactcaaca
     3781 tgatctgatt aaatatagaa aaataaactc taactgcaaa ctgtttgata atcctgttag
     3841 ttcttccttc ttttatacag ataaaaaatc ttcggctact gttaagaaat atatattaaa
     3901 tattggcagg ttggaaaaac agaagggaca ggatctactg ttagccgcat ggaatttggt
     3961 gaataaaaaa ggatatgaac tatggatttg cggagatggt agtcagaagc aaacactcga
     4021 acaacagaca aaactctatg gattaacgac atcagttaaa tttcttggaa atagagatga
     4081 tattgatatc ctactaagag gtgcatattg taatgtatta tcctctcgat ttgaaggaaa
     4141 gccagtgagt ctaattgagt caaaagttgt agggtgtcct aacatttcct ttaattgcaa
     4201 aactggtcca gctgagataa ttaatcataa atatgatgga ttattaattg atgcggggaa
     4261 tattgaggcg ttagccaaag gaatacagtt gttaatagac aatcctatta ttagagatga
     4321 gtatgcgaaa aatgcatgta tgaacaatga gagatatatg ctagaaaata tctgtgaaga
     4381 atatcttagc ttctttaata ttaaagcata aattatatta tgaatctaaa aagaaatggg
     4441 attttatttt acgctctaac tattttaaat ggtttggtgc ctcttataac aatatcgttt
     4501 atgtcaagaa tattaacaag tgatcaactt ggtaattatt tgctttcatt tacaatcatg
     4561 aatgtattag gcatggttgt agatttcggg atatcgattt caggtgttag aacattaaaa
     4621 aaaaataatc tcaatttaac acttgatgaa tttatttgtt cctccttttt attaaaattt
     4681 tttgtttttt tcttagtgtc aatatgctac ttggtatttt ttacttatac cgctactgag
     4741 gaggagatgt tttttatagt tactctatta ggagtattca ttacaggcat ggatagttta
     4801 tggatactgc aggtgagtgg tgatttatat aaaatactaa cgaaaaacat tatcgcaaat
     4861 atattatatg taatgatggt aatgttagtt gcatatataa ctaaagatta tttgagtaca
     4921 cttattttat ttgtaattta taagctttac atattattat taacaataaa cgaggttcgc
     4981 aaattatacg tcttatcaag aaataacttt agtataataa attttatttc tgtattcaag
     5041 agtacagctg gaatagctat gtttcgcttt tttgctcttt catatacctc ggcaaatggt
     5101 atcttattga attaccttac aaataaatat caggttgcgc tgtacttagc atttgaaaaa
     5161 ctaaataaag ctatactttt tatagcgacg cctcttacgc aagcattatt accattattt
     5221 gttgggggaa agaataataa caagttaata aaatacataa tatctatcat tattatttct
     5281 gtattcatat tagttattgg caacttatta tcaaaagaaa tcgtcggtct gtttataggt
     5341 acgagatatt taaatgatga tacatcagag ttcttttttt acatgacaat tgtttcgcca
     5401 ctaatacttt taagcaatgc gattggtatg ctttatttta ttccatgtaa acttgataga
     5461 tttcttaatt gtataatatg tagcgcagca tttataaata ttatttgtgt tttatatttt
     5521 gttcattcag atgtaaacgg agctttacag atggctcgtg ttgttgcatt tagtgaatta
     5581 tgggtaacgc tattcatggc ggtcgtggcg attatacatg ggagaaaata tggaaggtaa
     5641 aattttatat atatgctcgg atttttatcc gatgagtact ggttattcga atgcgttcag
     5701 taattttctg gatgcaattg gaaattatca agtagatata ttaacaacaa caaaagatgc
     5761 ctcattaaaa aaaagtgcta ttaatagagc aatttttaaa tctaaagtta tttatgggtg
     5821 tggtgcgttg gattatattg ttaactcttt aattttatat ggaaattaca ggaggttaat
     5881 tagaaaatat aattatgatc tcatattcat tgaaacattt gatgatgcat tatttttatc
     5941 attaataccc gagtcagaat acaaaacaat tattgttagg attcattcaa cttcagaaac
     6001 agaatataca tatttttttc caggtaaaaa atataaactc aacagattct tacaaagaaa
     6061 gataatagca ccgaagatta aaaatatatg ctctactagt gcatttcata atgggtttat
     6121 caaaaaaaat atgttttatg ataatcttta tgatatatgc cagaagaatt tttttgtgtt
     6181 gcctaataca atgcccgatg taaaaaattt tttttgtaaa gataaaaata atgaagaaaa
     6241 agttaagcta tttagcttag gtcgattgaa tagagaaggg atgtgtcaaa aaggtcaaca
     6301 tgatttaatt atggccatat ctttactagg taaaagcttt tcggatattg cttcatgtac
     6361 aattgtaggt gatggagaat attatgaata tctagagtca ttaatttaca aattaggact
     6421 tagcgaatgt atcaaactga ttcgtaagat gtctcatgat gaaataatta gtcacttaag
     6481 tttgtctgac attgtggtgt tgccatctcg ttttgaggga atgcctatgt ttgcccttga
     6541 agcattatat actcaaaatt tatgtctatt cagcaacaca ggtggtttaa gtgatatgat
     6601 agttaacgaa gattacagct accccccgca agatattgat gcattggcca aagcgattga
     6661 tcgggcaata cacttaaaca aaagcgattt gtataatgaa aagatgaatt catatgaaaa
     6721 ggcgctatct ttttcttatg aaaatgttaa aaataaattt gataaaatat ttaaaatggt
     6781 agtatcagga atatgattat ttttttacgt agttactcta tctccaaaga tagtcggctc
     6841 ttaaagtata tatctatatg caaagaaaat aatatacgtt ataagattat cggttgggat
     6901 aaagatcata acgaacaata tagagatgat gcctattatt ttaaaggtgg ctttaaactt
     6961 ggaaaaaaat ggctgaacgt gatggctatt tttaaatggt ggatctttat tttttatctg
     7021 attattttca ggataaaaaa taatattacc gccattcatt ctgtagatct tgattgtgga
     7081 attgttggtc taattataag taaattactt agagcaaaac atatatatga catatatgat
     7141 gtttattcgg ctaaccgtaa tatgagaggc cttattaagg cattgattga ttcacttgag
     7201 cggtttgtgt gtcggaagtc cacatatttt attatgcctg agtcattccg ttttgagcag
     7261 ttaggtatag accaagataa tatcaattat aataatttta ttgaaattga aaatgttcct
     7321 caaattgata ggaatattga attaagatac atatctcccg gtcagtatga tttttatctg
     7381 gcttatgctg gttcattaga accaagacac agaggattag agaacttatt gaaagctgta
     7441 tcaatttgtc ctaatgtatt cctcgacata gctggtgttg gaccattaac tgctttgtgt
     7501 aaaaaatact ctcagcaatt taataatatt tgttttcatg gtggtttaac acctcaacaa
     7561 gtctatgaat tagaggctaa cgcccaaatt attatcggta tgtactataa aacaagagat
     7621 aatcatcttt ttgcatctcc gaataaatat tatgagcact tggcacttgg taaagtcatg
     7681 ttgacaacca gcggcacccc tcctggaatg aaagttgaaa agtataaaac tggatttgca
     7741 attggtgaaa gtttacaaga cattatagat tttttacaat ctcttaatgg aaaagaggat
     7801 ataatagata atttttcaat aaatgcatat aggatctggg tagacaaata ttcggattac
     7861 aatgaaagat atttatctaa acaatacatg aaaatggtgg gtaagtgatg tacgattatt
     7921 taatagttgg ttcaggtctt tttggttcgg tatttgcata tgaaatgaaa aaaaaaggat
     7981 actcgataaa agtaatagaa aaaagagcgc acattggagg gaatatttat tgtgaggaaa
     8041 ttgaaggtat taatgtacat aaatatggtg ctcatatttt tcacactaat gaaaaagaaa
     8101 tctgggaata tgtgaattct tttgttgaat ttaatcgatt cactaattct ccggtagctt
     8161 tatctaaagg tgagatttat aacttaccat ttaatatgaa tacatttaat aaaatgtggg
     8221 gggtgacgtc tccctcagca gcaaaaaata aaattgaaca acagagaaaa gaaattggta
     8281 acaaagagcc agccaatttg gaagaacaag caatatcatt agttggtcga gatatttttg
     8341 aaactttaat aaaagaatat acagaaaaac aatgggggcg tgaatgcaaa aacttacctg
     8401 cattcattat taaacgtctt cctgttagat ttacctatga caataattat tttaatgatc
     8461 gatatcaagg aataccgatc ggtggatata atagaataat tgagaagatg ttagagggtg
     8521 tagacgttca attaaataca gacttttttg ataataaaga ttactggatt agtagcgcac
     8581 gaaaaattgt ttatacaggc cctattgatc gcttttttaa ttatgagttt ggtgagttgg
     8641 attaccgtac attatcatct gagcatgaga ttaagaatga aagtaatttt caaggaaatg
     8701 ctgtagttaa ttatattgat tcaagctatg aatttactcg aattattgag cataaacatt
     8761 ttgaatttgg agagcaggat aaaacagtta ttacgaaaga gtttcccaaa gagtatgtaa
     8821 atggagatga accgtactat ccggttaatg actctgaaaa taatagaaaa tatgccaaat
     8881 ataagttgaa aacaagccaa ttcgataacg ttatttttgg tggaagacta gcagaatata
     8941 agtattatga tatgcaccaa gtaattgcat ctgcattgaa agccgtaagt aaagaggttt
     9001 ctcaatgaaa atgcctagag ttatatgtat aattgtcact tttaatcgcg agcatttact
     9061 gcaacaaaca cttgatgcag tattaaatca aacattcccc ctacaaaata taattgttat
     9121 agataataat agcttcgata atacacgcag tttagtttct aaattgagcg aaaataatcc
     9181 caaaatagaa tatcataata ctggagcgaa tattggaggc gctggcgggt ttcatagagg
     9241 ttttgctgtt gcctcaaaat ataaatatga ttatttatgg cttatggatg atgatctaaa
     9301 gcctgaaata gaatgtcttt ataacctgat atcctctgca actgaacatg gtatctatca
     9361 acctataaga ataaatgtag atggctcatg tgcagaactt tcccctgttg tttatgattt
     9421 aaaaaatcct ttttttatga atccgaagcg attatctgtc atcaacctct ataaacgtat
     9481 gaacaatgct gtatatagtc cttttgatat tcatggagtt cctttcgagg ggccattaat
     9541 atctagagat gtaattcaat gtattggtta tcccaatcct aatttcttta ttttttatga
     9601 cgatcttgac tactccttga gggcaagaaa agcagggttt gaaatcaaat gtattccaag
     9661 tgccagagcg gttagactac taaaaaataa tcagcgtagt gatttgacat cttggaaagg
     9721 atattttatg tttcgtaatc ttttctatat tcatcgtacc tatggcgaaa atatattcgt
     9781 taggttaaaa ccatttctgc tggttctggg gtatcttgtg tttttaatct ttagatttaa
     9841 gtttaatcaa gttcctacta ttttatctgc atttaaagat tcacgaaaac ttgataattc
     9901 tgaattacat aaaccataaa agggttttat aattgccaat gttaactgta attaaaccat
     9961 aatgctaaca aacccaaaca tggtgtatca taaaatgatt ttattagtta ataaaaagta
    10021 agttttttac atgacgtagc ttatttgcac atatgagatg tgcctagaat ataatatact
    10081 catttctgtg tgaatataaa atttatacct acgcttactt agtttgtata attatcttga
    10141 tgagctaaga gataatataa tttgcaaaat taatttattg tgaattaatt tgataatgct
    10201 aatgcataga cataacttgt aaaattaaac atcagtttgt tttttttatt aatctacatc
    10261 attaagaata aggatttggc attgtgggtt taataacatc atagtattat tcactcttac
    10321 tatttatagg ttggcatata acgaaaaatg ttaattgata gactgatatg tagatattca
    10381 tttctcatgt taatgagatt ggaataatgt gttgttaatt tgtcttgttt ataaatttac
    10441 ttactgatat ttgtgattat tcatcatata acttcttgta aatgagcatc ctctagcaat
    10501 taatgttgtc cttcataagc aagctgcatc ttcgcggcat tttccgaaag gagtaaacaa
    10561 tgtcaaagca acagattggc gtagtcggta tggcagtgat ggggcgcaat cttgcgctta
    10621 acatcgaaag ccgtggttat accgtctcta ttttcaaccg ttcacgtgag aaaacggaag
    10681 aagtgattgc cgaaaatcca ggcaaaaaac tggttcctta ctatacggtg aaagagtttg
    10741 ttcaatctct ggaaacgcct cgtcgtatcc tgttaatggt gaaagcaggt gcaggcacgg
    10801 atgctgctat tgattccctc aagccatacc tcgataaagg tgacatcatc attgatggtg
    10861 gtaacacctt cttccaggac actattcgtc gtaaccgtga gctttctgca gaaggcttta
    10921 acttcatcgg taccggtgtt tccggtggtg aagaaggtgc gctgaaaggt ccttccatta
    10981 tgccaggtgg acagaaagaa gcctatgaac tggttgcgcc gattctgacc aaaatcgccg
    11041 cagtggctga agacggtgag ccatgcgtta cctatattgg tgccgatggc gcaggtcact
    11101 atgtgaagat ggttcacaac ggtattgaat acggcgatat gcaactgatt gctgaagcct
    11161 attctctgct taaaggtggc ctgaaccttt ccaacgaaga act
//
