LOCUS       AOMX01000002            6585 bp    DNA     linear   BCT 04-DEC-2017
DEFINITION  Escherichia coli O157:H7 str. TW14313 Contig2, whole genome shotgun
            sequence.
ACCESSION   AOMX01000002
VERSION     AOMX01000002.1
DBLINK      BioProject: PRJNA187385
            BioSample: SAMN05001776
KEYWORDS    WGS.
SOURCE      Escherichia coli O157:H7 str. TW14313
  ORGANISM  Escherichia coli O157:H7 str. TW14313
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6585)
  AUTHORS   Abu-Ali,G.S., Kotewicz,M.L. and Elkins,C.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2013) US Food and Drug Admin., 8301 Muirkirk Road,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Velvet v. 1.2.07; Bowtie v. 2
            Genome Coverage              :: 173.0x
            Sequencing Technology        :: Illumina GAIIx
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI
            Annotation Date              :: 10/29/2014 09:31:17
            Annotation Pipeline          :: NCBI Prokaryotic Genome Annotation
            Annotation Method            :: Best-placed reference protein set;
            Annotation Software revision :: 2.8 (rev. 450396)
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA;
            Genes                        :: 5,683
            CDS                          :: 5,407
            Pseudo Genes                 :: 138
            CRISPR Arrays                :: 1
            rRNAs                        :: 22 (5S, 16S, 23S)
            tRNAs                        :: 104
            ncRNA                        :: 12
            Frameshifted Genes           :: 53
            ##Genome-Annotation-Data-END##
            Annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (released 2013). Information about the Pipeline can be
            found here: http://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria available from US Food and Drug Admin, Laurel MD.
            Pipeline
            GeneMarkS+
            repeat_region
FEATURES             Location/Qualifiers
     source          1..6585
                     /organism="Escherichia coli O157:H7 str. TW14313"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Contig2"
                     /strain="TW14313"
                     /serovar="O157:H7"
                     /host="Homo sapiens"
                     /db_xref="taxon:1286877"
                     /country="USA"
                     /collection_date="2006"
     gene            1..1050
                     /locus_tag="H260_21035"
     CDS             1..1050
                     /locus_tag="H260_21035"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_000445155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="PJR32594.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWEI
                     GRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEIRQ
                     TVSGELTPLPWSTYVRLLSVKNADTRSFYEKETLRCGWSVRQLERQIATQFYERTLLSH
                     DKSAMLQQHAPAETHILPQQAIRDPFVLEFLELKDEYSESDFEEALINHLMDFMLELGD
                     DFAFVGRQRRLRIDDNWFRVDLLFFHRRLRCLLIVDLKVGKFSYSDAGQMNMYLNYAKE
                     HWTLPDENPPIGLVLCAEKGVGEAHYALAGLPNTVLASEYKMQLPDEKRLADELVRTQA
                     VLEEGYRLR"
     gene            complement(1110..1574)
                     /locus_tag="H260_21040"
     CDS             complement(1110..1574)
                     /locus_tag="H260_21040"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_001480287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="PJR32595.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDNI
                     FMNVMTFNTQSPVEKKAELHEQYIDIQLLLKGEERILFGTAGTARQCEEFHHEDDYQLC
                     SAIENEQTINLKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(1571..2446)
                     /locus_tag="H260_21045"
     CDS             complement(1571..2446)
                     /locus_tag="H260_21045"
                     /EC_number="2.7.1.60"
                     /inference="EXISTENCE: similar to AA
                     sequence:SwissProt:Q8X9H0.2"
                     /note="catalyzes the phosphorylation of the
                     N-acetylmannosamine (ManNAc) liberated from
                     N-acetyl-neuraminic acid by the nanA protein; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="PJR32596.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDAL
                     SALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLGQLTDLPTIAIN
                     DAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLADPHG
                     PVCGCGRTGCVEAIASGRGIAAAAQGELAGADAKTIFMRAGQGDEQAQQLIHRSAHVLA
                     RLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHDAGLLG
                     AALLAQGEKL"
     gene            complement(2443..3132)
                     /locus_tag="H260_21050"
     CDS             complement(2443..3132)
                     /locus_tag="H260_21050"
                     /EC_number="5.1.3.9"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_020231101.1"
                     /note="Converts N-acetylmannosamine-6-phosphate to
                     N-acetylglucosamine-6-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="PJR32597.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQA
                     GAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADIIAI
                     DGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTPETP
                     EEPDLTLVKRLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQWYNT
                     AMKKAVL"
     gene            complement(3180..4670)
                     /locus_tag="H260_21055"
     CDS             complement(3180..4670)
                     /locus_tag="H260_21055"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_001701158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P41036|2.A.1.12.1"
                     /protein_id="PJR32598.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLTE
                     VQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLACGF
                     APGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVAAQV
                     YSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILYRGEH
                     RIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCTAIFISFMVQSTGKRWPTGVMLM
                     VVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFLGDWLGT
                     RKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIGGYFDTDQ
                     RAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLIGLDMPSRV
                     QRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4779..5672)
                     /locus_tag="H260_21060"
     CDS             complement(4779..5672)
                     /locus_tag="H260_21060"
                     /EC_number="4.1.3.3"
                     /inference="EXISTENCE: similar to AA
                     sequence:SwissProt:P0A6L4.2"
                     /note="catalyzes the formation of pyruvate and
                     N-acetylmannosamine from N-acetylneuraminic acid; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH33"
                     /protein_id="PJR32599.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYVG
                     GSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDAVS
                     AVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPGVGA
                     LKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQGIVK
                     ALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGPVDEKY
                     LPELKALAQQLMQERG"
     gene            complement(5794..6585)
                     /locus_tag="H260_21065"
     CDS             complement(5794..6585)
                     /locus_tag="H260_21065"
                     /inference="EXISTENCE: similar to AA
                     sequence:RefSeq:WP_000523840.1"
                     /note="Transcriptional repressor of the nan operon that
                     encodes proteins involved in sialic acid utilization;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DBD-Pfam|GntR,DBD-SUPERFAMILY|0037767"
                     /protein_id="PJR32600.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMIR
                     RREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADTII
                     GELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQSLD
                     NNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVSYQQH
                     IAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
ORIGIN
        1 atggaatcgc tctcagagag aacctcaaca ggctaccagc aaatccacga cggtattatt
       61 cacctggtcg atagcgcccg gacggaaacg gtacgtagcg ttaacgcgct gatgaccgcg
      121 acatactggg aaattggccg acgaattgtc gaatttgaac aaggtggcga ggccagggct
      181 gcgtatggtg cgcagctaat caagcgacta tcaaaggatt taagtctaag gtataagcgt
      241 gggttctctg caaaaaactt acgccaaatg aggctttttt acctcttttt tcaacatgtt
      301 gaaattcgcc agacagtgtc tggcgaatta acaccattgc cctggtccac ttacgtccgt
      361 ttactttccg ttaaaaacgc tgacacccgc agcttttatg aaaaagagac gctccgctgt
      421 ggctggtctg ttcgccagct agaacggcaa attgcgaccc aattttatga gcgaacactg
      481 ctgtcacatg acaaatcagc catgctgcaa caacacgctc ctgccgagac gcatattctt
      541 ccgcaacagg cgatacgcga tccctttgtg ctcgaatttc tggaattgaa agatgaatac
      601 tcagaatccg attttgagga ggcgctgatc aaccacctga tggatttcat gctggaactt
      661 ggggatgatt ttgcctttgt tggtcggcag cgaaggttac gcattgatga caactggttt
      721 cgtgtcgatc tgctgttttt ccaccgccgt ttacgctgcc tgctaatcgt cgatctaaaa
      781 gtgggcaaat tcagctatag cgatgccgga cagatgaata tgtatctcaa ctacgccaaa
      841 gagcactgga cgctaccgga tgaaaatccg cccatcggtc tggttctctg tgcagagaaa
      901 ggagtcggag aagcgcatta tgcactggct ggtttgccta acaccgttct ggcgagtgaa
      961 tataagatgc aactacctga tgaaaaacga ctcgcggatg aactcgttcg aacacaggcg
     1021 gtgctagaag aaggctatag actccgttaa tttcagcggt ttcgaattct gcagacactg
     1081 tctgcagaat ctttaaaacg aactaagttt taagccatta aatcagcctt aaccttcacc
     1141 accacctttt taatctcacc aggttcgccg acaacgcatc ctggtttatg cggttctccc
     1201 ggcataaaca cggcgaacat tcccggtttt aagttgatgg tttgctcgtt ttcaatggcg
     1261 ctgcaaagct ggtaatcatc ttcatggtgg aactcttcac actggcgcgc agtgcctgcc
     1321 gtgccaaaca gaatccgttc ctcacctttt aataacagct ggatatcaat gtattgctcg
     1381 tgcaattccg cttttttctc gacgggcgat tgagtgttaa acgtcatgac attcataaaa
     1441 atattgtcgc cctgtaattc gtaacgaccc ggcgcttttt cttgcggtct ggcagctaat
     1501 gccagcgtta acgcgtcctg taacgcagga tgtaacccag cagacggtaa tgactgtact
     1561 tcacccatca tcataatttt tctccctggg ccaacagcgc agccccaagt aaacctgcat
     1621 catggcggta gtgcgccgcc agtaaatcaa catgaaatgc cgctggctcc tgcgccagat
     1681 acgtttccac cagcgccaga tacccttctg ccagaccaac gctgccaccg accaccacgc
     1741 actgacaatc agttgtggct ttaatatcag cgatcagcct tgccagcaca tgtgcggagc
     1801 ggtgaatcag ctgctgcgcc tgctcgtcgc cctgcccggc gcgcatgaaa atagttttcg
     1861 catccgcgcc agccaactcc ccctgcgctg ccgctgcaat accgcgacca gaggcaatcg
     1921 cttccacgca acctgtgcgt ccacagccgc agactgggcc gtgtggatcg gcaagcgtat
     1981 gcccgatgtg ccccgccaga ccgccagggc cggtaagcag tttgccgccg ctcactacac
     2041 cgccgccgac gccagtggaa acggtgataa acaccatatc ggttatatcg ccttccagcg
     2101 cctgatactc tgcccatgct gcggcctggg cgtcgttaat ggcaatggtc ggcaaatcgg
     2161 taagttgccc cagcgttttg actaacggaa agtgtagcaa tccaccaaga ttatgcggat
     2221 tcagcgccag caaactgccg tcgcgaataa ttccggttga agcgatggca acccgctgcg
     2281 catgagcttg caacggagag actaatgcgg ataaggcatc acgcaaggct tctggtgtct
     2341 ggctggctgg cgtaggaagt tcacgacgat cgcggatctg cccgtcagcg ccaatcagcg
     2401 cggcggcaag tttagtaccg ccgatatcaa tcgccagtgt ggtcatagca ccgccttttt
     2461 catcgctgtg ttgtaccact gacaaatgtg ttcaagacgc gtaatggcag aaccgaccgt
     2521 caccgcccac gcgccgtggc gcatcgcatc agccgcctga gcaggcgtgt tgtaacgccc
     2581 ttcggcaatc acccgacatc cggcgtcgct caaccttttc accagcgtca gatccggctc
     2641 ttctggcgtt tcaggcgtgg tatagccaga aagcgtagtg ccaataattt cggctcccag
     2701 cttttggcat gccaggccgt cttccggcgt tgagcagtcg gtcatcgcca gtaaaccgtg
     2761 atgatgaata cgtgccagca gcgtttcaac aggcaccgga cgcgggcggt cggtgccgtc
     2821 aatggcgata atgtccgcgc ccgcctgcgc cagcgcatcg acatcttcaa tataggccgt
     2881 gatgcgtacc ggagaatcct ccagatcgcg tttcacaatt ccaataatcg gcacgctcac
     2941 caccgcacgc gtggcttgca gatttgccac accttcgatg cgaatggcaa ccgcgcccgc
     3001 ctgctctgcc gctaatgcca tggctgcgac gatttcgggt ttatcgagcg ggctgtccgg
     3061 aaccggctgg caggagacaa tcaggccacc gttagcagcg attttttgat ccagttgtgc
     3121 aagtaacgac atacatcttc ccttagcgaa aggcccgata cagagaccgg gcaacaggat
     3181 taacttttgg ttttgactaa atcgtttttg gcgctgccaa acggcacggc accgctaaat
     3241 ggtttgccgt cgatagcgtc atgagtacgc aacgcttccg ggcgcagcca acgctgaaca
     3301 cgagaaggca tatccagacc aatcagcagg atcaccacaa atgtcagact gaacgagagc
     3361 gatgccagtg cagtacccag atccagacgt tgagcgatca acgcgccgat gattggggcc
     3421 agtgcgccac ccaatgcgcc aacgttatag gtaaagccca ggcccgctgc acgctggtcg
     3481 gtatcgaaat aaccgccaat cagtttcggt aagatcccgg cgatcccttg tccaagcatt
     3541 tgctggaaga acagtaacag accgagcacc cagacgtttg caccgccaat agcaaatacc
     3601 ggaataatca gcagctgcga ggccaacaga ctacaaacgt acgctttgcg ggttcccagc
     3661 cagtcaccga ggaagccacc gacgcagcac cccaccgccg cgccaaagcc actaaagaac
     3721 agcacattgg cgacagtatg agggttataa gccagatcgg ttttcagata cgttggcagc
     3781 agcgcctgaa tcggccatga gtagaggaaa gcaaacaaca cgaccaccat cagcattacg
     3841 ccagttggcc agcgtttgcc cgtactctgc accataaagc tgataaagat tgcggtgcat
     3901 aacagcccaa gaacagcgac gatcgcggca ttttgcaggt taccggcgaa gcagaaccac
     3961 agcgcagtag ccgccgccag tgtcattacg atattggcaa tgcgatgttc accacggtag
     4021 agaatatcca ccattgtgcg tactggtgct ttacctgcgt gtttctcttt ccagtcttct
     4081 gcttccggga tgtttttacg cagccagaga gcaaagatga ttggcaaaat gccgataaag
     4141 aacagcgcac gccagcccca gaccggaacc accaggctgt agacctgagc ggcaacgacg
     4201 gcccctacag agaagcctga aatcagaaag ccactggctt tgttacgcag atgttttggc
     4261 cagctttcaa tgacataggt ggcgctggaa ccgtattcac ccgccatccc catgccgatg
     4321 accagacgag cgataaacat ggtgatgtag cctggcgcaa agccgcatgc cagcgtcccg
     4381 gccgagaaga gaacgatgct ggtgaccatt gccagacgac gcccgtagcg gtcacccata
     4441 gcgccgagca tcaggccgcc gaaccagcga gagataaagg ctgcggagat cagacttgcc
     4501 gcctgcaccg tcgtcagccc gaattcacct tgtacttcgg tgagtaccag ggcgattaaa
     4561 acgaaatcaa aaccgtcaag cagatatccc aaccaggcag cggaaaatgc ccgccattgt
     4621 gcacgattga gatggcgata ccacgggata ttctgggttg tagtactcat tgtgagtctc
     4681 ccgcggtggg cgatgcccac acgctttggt atgaaaattg tagggtacag atgcgtttat
     4741 ttcccctcac ccggtagggg cgagcgaggg gaaacaactc acccgcgctc ttgcatcaac
     4801 tgctgggcca gcgccttcag ttctggcaga tatttttcat ctaccggacc aaacggtttg
     4861 cggcacagcg gcacagaaac gacatccata taatgcagaa cagttttcag gccgcggaat
     4921 acacccgttt tgatcagtaa atcaatgact ttgttgcatt cagtttgcag tttctgcgcg
     4981 gtctggatat cgccttcttt cagcgcctta acgatcccct gatagcgcca gcccatgatg
     5041 ttgtaggtac taccgatacc accatcagcg cccgccagca ggccagaggc gaagatttcg
     5101 tcgtaaccgt tatagagcac cagatcagga tgttcacgac ggatctgctc catctgatag
     5161 agatcgccag aggtctgttt cagcgcacct acgccaggca atgtaacaag tgtgttgatc
     5221 tgatccaggg tcagttttac cccactcagg gctggaatgt tgtacaccac catcggcaaa
     5281 ccatccgccg aatcaataat tgcccgatag tgatcgcagt gttcttcaaa gctgaaagga
     5341 tagtagaacg gcgtgacggc ggagacggca tcgaagccat aacgtttagc cgatgccgca
     5401 agttgttggc tttcggcggt gctgacgcaa ccgacgtggg cgatgagttt aatcttacct
     5461 ttcgcctctt cggcgacgat ttccagtacc tgttcacgct cggaaaggct ttgtacaaag
     5521 gcttcgccgg tcgaaccacc cacgtataaa ccgtcaatgc cctgctgaat attgaactga
     5581 accaggcggc gcagactcgc tttatccagt gcttgttgtt gatcaaaagg agtcaggagt
     5641 gcagccatta cgccacgtaa attcgttgcc ataaatacct ctgaagtgat gcttgtctga
     5701 taaacgatat acctttatac ctgttatacc agatcaatta agcaacaccc catacagaaa
     5761 gcttataatg cgatctgctt cactaaagtg gcattatttc tttttgttgg tggtctgacc
     5821 gaaggcgtgc caggtagcag agacgctgtt gagatgcgat tgcaacgcac gatcggcttc
     5881 gtcaggatcg tggcggcgga tcgcgtcaac gatcgcaata tgctgttgat agctaacgtt
     5941 gttatgctcg tgcagagcct gatcagccac ggttggacgt gcggcgatca gccagtcgag
     6001 cagggcaacg tggatcgcca tgaagattgg gttaccgggg atctccgcca gcacgcggtg
     6061 gaaatcaacg tctgagcgaa tgaatgccgc gttgttatcc agcgactgac tgttgatttc
     6121 cagtgctttt gccagcaaat cgatttgctc atcggtggcg tgttcagccg catagcgcac
     6181 caggctggat tcaaagaaca gacgtaattg ttcgaaatgg gcaatcccac caggatgaga
     6241 aaggaaatct ttcgccatgc cggaaagctc gccgatgatg gtgtccgcag aaggacgtga
     6301 gacgcgggca cgttcgccgt tgtttatttg caccagacct ttgcgtttta acgctgccag
     6361 cgcttcacgc accgaaggac gcccgacgtt aaagaacgcc atcagttcgc gttcagacgg
     6421 taattgttca ccttcgccaa attcacgacg gcggatcatc tgttccagct cttcttccac
     6481 catttcggag agttttttac gcgccagcgg gcggctacgc aagttgcgac caattgcagg
     6541 tgaagaatct tcggtttgcg aatcaaatgc gttcataagg cccat
//
