LOCUS       AF417111                3969 bp    DNA     linear   BCT 20-OCT-2003
DEFINITION  Ralstonia solanacearum PehC (pehC) and ExuT (exuT) genes, complete
            cds.
ACCESSION   AF417111
VERSION     AF417111.1
KEYWORDS    .
SOURCE      Ralstonia solanacearum K60
  ORGANISM  Ralstonia solanacearum K60
            Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Ralstonia.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Gonzalez,E.T. and Allen,C.
  TITLE     Characterization of a Ralstonia solanacearum operon required for
            polygalacturonate degradation and uptake of galacturonic acid
  JOURNAL   Mol. Plant Microbe Interact. 16 (6), 536-544 (2003)
   PUBMED   12795379
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Gonzalez,E.T. and Allen,C.
  TITLE     Identifying the role of pectolysis during Ralstonia solanacearum
            pathogenesis on tomato
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3969)
  AUTHORS   Gonzalez,E.T. and Allen,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2001) Plant Pathology, University of
            Wisconsin-Madison, 1630 Linden Drive, Madison, WI 53706, USA
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Ralstonia solanacearum K60"
                     /mol_type="genomic DNA"
                     /strain="K60"
                     /db_xref="taxon:1091042"
     gene            170..2209
                     /gene="pehC"
     CDS             170..2209
                     /gene="pehC"
                     /note="exo-polygalacturonase"
                     /codon_start=1
                     /transl_table=11
                     /product="GH28"
                     /protein_id="AAL24033.1"
                     /translation="MPKQEHSSARAAGRQSRHRSHSPARRAFVVWSGASAGAALLGALP
                     GCGGDGGSDATVGTASTADATATHHADAIWGESGAVARIIASLQGVARSMFPERDFVVT
                     DYGAQPCAIVDAVNPYTDASKSPLSAGADKTPASGAFDSRPAFTAAIAACNAAGGGRVV
                     VPAGNWYCAGPIVLLSHVHFHLGADCTIYFSPNPDDYAKDGPVDCGTNGKLYYSRWQSN
                     DCLNYGAPIYARNQSNIALTGEGDSSVLNGQAMTPFAGSGNTSMCWWTFKGTKGAYGVV
                     DASTPSQASGNPNNVDLRTAAPGIADALYAKLTDPATPWQQDQNYLPALSEAGVAVAQR
                     IFGKGHYLRPCMVEFIGCTNVLMETYRTHATPFWQHHPTDCTNVVIRGVTVDSIGPNND
                     GFDPDACDNVLCEGMTFNTGDDCIAIKSGKNLDTAYGPAQNHVIQDCIMNSGHGGITLG
                     SEIGGGVQQIYARNLTMRNAFYATNPLNIAIRIKTNMNRGGYVRDFHVDNVTLPNGVSL
                     TGAGYGSGLLAGSPINSSVPLGVGARTSANPSASQGGLITFDCDYQPAKDAIRTRPAQV
                     QNIHISNVRASNATVGGTTGSCFQAIVAQGPVAFDYNGPTPAPAAPPIAGVTITDCDFG
                     SPVAAGPAGATAPGPIYAYNVSGITLTNVRIGTQLYNTTVSDTR"
     gene            2285..3605
                     /gene="exuT"
     CDS             2286..3605
                     /gene="exuT"
                     /note="galacturonate transporter"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|P0AA78|2.A.1.14.2"
                     /protein_id="AAL24034.1"
                     /translation="MNTIKGLRWWMIGLVLTGLIMNYLARNALAAAAPEVNKVLNITTQ
                     QYGYIVAAFQAAYMVMQPVAGYVLDMLGTKLGFALFAGAWSVVCILHSTAGNWVSLAVF
                     RALLGVTEAAGFPSALRATSEWFPAKKRSIATGWFNIGSSVGALVAPPLVVWCILHGDW
                     RLAFSAIGALGVVWSVLWFTLYRVPAQHPRLSADKHDYIRAGQEAPDDRAGTAKPSWKA
                     IITSRRFWGIAIPRFLSEPAWQTFNFWIPLYMATQRHMNLKEIAMFAWLPFLAADVGCV
                     LGGYLAPWYQKRFSTSLITSRKMVMVTGAVCMIGPACIGLATSPYTAIALFCVGGFAHQ
                     TLSGALYTLTADVFGKHEVGTAVGLAGMSGFLGGTLFSLAVGALASTIGYNPLFAALAL
                     FDIVAAIVMWNVLREPRGPIAMPTGAGAADLGAAPRAAGG"
ORIGIN
        1 cgatgtgtgc gttcggcggg cccgggctgg acaccttgtt cgtgacctcg atccgcgtgg
       61 aaggcgatgc gctgtcgggc ggcaccttcg ccctgcgtcc tggcacacgg ggcctcgatg
      121 aaccaccatt gcgcatggat tgacccctga actttcacag gagaaagaca tgcccaaaca
      181 ggaacactcc agcgcgcgcg ctgccggccg acagtcgcgg catcgctcgc attcgccagc
      241 gcggcgcgca ttcgtcgtct ggtcgggcgc atcggccggc gccgcgctgc tcggcgcgct
      301 gccgggctgc ggcggcgatg gcggcagcga cgccacggtc ggcacggcca gcaccgcgga
      361 tgcgacggca acacaccacg cagacgcgat ctggggcgaa tccggcgcgg tcgcgcgcat
      421 catcgcctcg ctgcagggcg tggcccgatc gatgtttccc gagcgcgact tcgtggtgac
      481 ggactacggc gcccagcctt gcgccatcgt cgatgcggtc aacccctata ccgacgccag
      541 caaatcgccg ctcagcgcgg gcgccgacaa gacgcccgcg agcggcgcct tcgattcgcg
      601 ccccgcattc accgccgcca tcgccgcctg caacgcggcc ggcggcgggc gggtggtggt
      661 gccggccggc aactggtact gcgccggccc gatcgtgctg ctcagccatg tccacttcca
      721 cctgggcgcc gactgcacca tctacttcag ccccaacccg gacgactacg ccaaggacgg
      781 cccggtcgac tgcggtacca acggcaagct ctattacagc cgctggcagt caaacgattg
      841 cctgaactac ggcgccccca tctacgcgcg caaccagagc aatatcgccc tgaccgggga
      901 aggcgacagc tccgtcctca acgggcaggc catgacgccg ttcgcgggca gcggcaacac
      961 cagcatgtgc tggtggacct tcaaaggcac caagggcgca tacggcgtcg tcgatgcttc
     1021 cacgccgtcg caggcatccg gcaacccgaa caacgtcgac ctgcgcacgg cggcgccggg
     1081 catcgccgat gcgctttacg ccaagctgac cgatccggcc acgccctggc agcaggacca
     1141 gaactacctg cccgcgctgt ccgaggccgg ggtggcggtg gcgcagcgca tcttcggcaa
     1201 gggccactac ctgcggccgt gcatggtcga gttcatcggc tgcaccaacg tgctgatgga
     1261 gacctaccgc acccacgcca cgccgttctg gcagcaccat ccgaccgact gcaccaacgt
     1321 ggtcatccgc ggcgtgacgg tcgacagcat cggccccaac aacgacggct tcgatccgga
     1381 cgcgtgcgac aacgtgctgt gcgagggcat gaccttcaac acgggcgacg actgcatcgc
     1441 catcaagtcc ggcaagaacc tcgacaccgc ctacggcccg gcgcagaacc acgtgatcca
     1501 ggactgcatc atgaacagcg gccacggcgg catcacgctc ggcagcgaga taggcggcgg
     1561 cgtgcagcag atctacgcgc gcaacctgac gatgcgcaac gcgttctatg cgaccaatcc
     1621 gctgaacatc gccatccgca tcaagaccaa catgaaccgc ggcggctacg tccgggactt
     1681 ccatgtcgac aacgtgacgc tgcccaacgg cgtcagcctt accggcgccg gctacggcag
     1741 cggcctgctg gcgggcagcc cgatcaacag cagcgtgccg ctgggcgtgg gcgcacgcac
     1801 cagcgccaat ccgtccgcgt cgcagggggg gctcatcacc ttcgattgcg attaccagcc
     1861 ggccaaggac gccatccgca cacggcccgc gcaggtgcag aacatccata tctccaacgt
     1921 gcgcgcgtcc aacgcgacgg tgggcggcac gacggggtcg tgcttccagg ccatcgtcgc
     1981 gcaagggccg gttgccttcg actacaacgg cccgacgccc gcgccggcgg ctccgcccat
     2041 cgcgggcgtc acgatcaccg actgcgattt cggcagcccg gtggcggccg gcccggccgg
     2101 cgcgacggcc ccgggcccga tctacgccta caacgtcagc ggcatcacgc tcaccaacgt
     2161 cagaatcggc acccagctct acaacaccac ggtgagcgac acacgctgac ggaccggcac
     2221 cgcggcagcc cagatatcac aacacgccaa caggcgggat catcaaggag gcgacaggag
     2281 gcgacatgaa cacgatcaaa gggctgcgct ggtggatgat cgggctggtg ctgaccggcc
     2341 tgatcatgaa ttacctggcg cgcaacgcac tggcggccgc tgcacccgaa gtcaacaagg
     2401 tgctcaacat cacgacgcag cagtacggct acatcgtcgc ggcgttccag gcggcgtaca
     2461 tggtgatgca gccggtggcg ggctacgtgc tggacatgct cgggaccaag ctgggctttg
     2521 cgctgtttgc cggggcgtgg tcggtggtct gcatcctgca cagcacggcg ggcaactggg
     2581 tgtcgctggc ggtgttccgc gccctgctgg gcgtgacgga ggccgccggc ttcccgtccg
     2641 ccctgcgcgc gacctcggaa tggttcccgg ccaagaagcg ctcgatcgcc accgggtggt
     2701 tcaacatcgg ctcgtcggtg ggtgcgctgg tggcaccccc cctggtggtc tggtgcatcc
     2761 tgcatggcga ctggcggttg gccttttccg cgatcggcgc gctcggggtg gtgtggagcg
     2821 tgctgtggtt cacgctctat cgcgtgccgg cccaacatcc gcgcctgtcg gcggacaagc
     2881 acgactacat ccgcgccggg caggaggcgc cggacgatcg tgcgggcacc gcgaagccgt
     2941 cgtggaaagc gatcatcacc agccgccgct tctggggcat cgccatcccg cgcttcctgt
     3001 cggagccggc gtggcagacc ttcaacttct ggatcccgct ctacatggcg acgcagcggc
     3061 acatgaacct caaggagatc gccatgttcg cgtggctgcc gttcctggcg gcggacgtcg
     3121 gctgcgtgct gggcggctac ctggcgccgt ggtaccagaa gcggttttcc acgtcgctga
     3181 tcacctcgcg caagatggtg atggtgacgg gcgcggtctg catgatcggt ccggcctgca
     3241 tcggcctggc gacaagccct tacaccgcga tcgcgctgtt ctgcgtcggc ggctttgcgc
     3301 accagaccct gtcgggcgcg ctgtacacgc tgaccgcgga tgtgttcggc aagcacgagg
     3361 tcggcacagc ggtggggctg gcgggcatgt cgggcttcct gggcggcacg ctgttctcgc
     3421 tggccgtcgg cgcactggca tcgaccatcg gctacaaccc gctgttcgcg gcgctggcgc
     3481 tgttcgatat cgttgccgcg atcgtcatgt ggaatgtcct gcgcgagccg cgcggtccga
     3541 tcgcgatgcc gacaggggca ggcgccgcag acctgggcgc cgccccgcgt gcggccggcg
     3601 gctgacgccg tcgcgttgtt tgatcgaatc ttcatttgac gcgccgtcgg cgggcggcgt
     3661 accatgcctg cacggctgac ggacagaccc gacgccgcca atctcttgag ggagcctcat
     3721 gagcaccaac accaagcgtt tcgacgcgct ggttttcatc ggtcgtttcc agccgctgca
     3781 tcgtgcacac atcgaagtgc tgcgccgggc cctgacgcct tgccgacacc gtctgcgtgc
     3841 tgatcggctc gaccgacaag ccgcgcacca tcaaaaaccc gttttcttcg acgaacgccg
     3901 gcagatgatc cttgtccgtg ctgaacgagg ccgaccgcga gcgcgtgagg atcgccgccg
     3961 tccaagatt
//
