LOCUS       KJ778804               12933 bp    DNA     linear   BCT 29-MAR-2016
DEFINITION  Escherichia coli strain P 9b serotype O69:K-:H38 O-antigen gene
            cluster, complete sequence.
ACCESSION   KJ778804
VERSION     KJ778804.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12933)
  AUTHORS   DebRoy,C., Fratamico,P.M., Yan,X., Baranzoni,G., Liu,Y.,
            Needleman,D.S., Tebbs,R., O'Connell,C.D., Allred,A., Swimley,M.,
            Mwangi,M., Kapur,V., Raygoza Garay,J.A., Roberts,E.L. and Katani,R.
  TITLE     Comparison of O-Antigen Gene Clusters of All O-Serogroups of
            Escherichia coli and Proposal for Adopting a New Nomenclature for
            O-Typing
  JOURNAL   PLoS ONE 11 (1), E0147434 (2016)
   PUBMED   26824864
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12933)
  AUTHORS   Yan,X., Fratamico,P.M., Tebbs,R.S., O'Connell,C.D., Swimley,M.,
            Baranzoni,G.M., Debroy,C. and Liu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2014) Molecular Characterization of Foodborne
            Pathogens Research Unit, USDA-ARS, 600 East Mermaid Lane, Wyndmoor,
            PA 19038, USA
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: CLC Genomics Workbench v. 7.0
            Coverage              :: >50X
            Sequencing Technology :: IonTorrent
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="P 9b"
                     /serotype="O69:K-:H38"
                     /db_xref="taxon:562"
     misc_feature    1..12933
                     /note="O-antigen gene cluster"
     gene            108..1193
                     /gene="rmlB"
     CDS             108..1193
                     /gene="rmlB"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="AIG62806.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLAD
                     VSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIVGT
                     YVLLEAARNYWSALDGDKKNSFRFHHISTDEVYGDLPHPDEVNNKEQLPLFTETTAYAP
                     SSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEGKALP
                     IYGKGDQIRDWLYVEDHARALYIVVTEGKAGETYNIGGHNEKKNIDVVLTICDLLDEIV
                     PKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWYLSNTKW
                     VDNVKSGAYQSWIEQNYEGRQ"
     gene            1193..2092
                     /gene="rmlD"
     CDS             1193..2092
                     /gene="rmlD"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="AIG62805.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPEG
                     VAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHYST
                     DYVFPGTGEIPWQEADATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGNNFA
                     KTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVASGTTT
                     WHDYAALVFEEARKAGIPLALNKLNAVSTTAYPTPARRPHNSRLNTEKFQQNFALVLPD
                     WQVGVKRMLNELFTTTAI"
     gene            2150..3028
                     /gene="rmlA"
     CDS             2150..3028
                     /gene="rmlA"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="AIG62804.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTLM
                     LAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGGDD
                     CALVLGDNIFYGHDLPKLMDAAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISLEEK
                     PLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMMGRGY
                     AWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFINAEQVKILAEPLKKNAY
                     GQYLLKMIKGY"
     gene            3033..3578
                     /gene="rmlC"
     CDS             3033..3578
                     /gene="rmlC"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="AIG62803.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFFESFNQKVFEEAVGRKVEF
                     VQDNHSKSCKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVNLS
                     AENKRQLWIPEGFAHGFLVLSDEAEFVYKTNNYYYQKHERSILWNDKILNVEWPFTSNL
                     ILSSKDMSASLFTVAEKF"
     CDS             3596..4951
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-reducing hydrogenase subunit beta"
                     /protein_id="AIG62802.1"
                     /translation="MSIQNVIDNGYCVGCGACKLALGESVEIKMYEDGFYNASIKPNAN
                     IEIAEKICPFSDSSTNETILGASLYGDKKFDKRVGYFDDIYIGYVVDNQDRLTSSSGGL
                     TTWFAKKLLINKEVDAVIHVGHGTHMFDYIITENILDLDLQNNKKSRYYPVSFSELINK
                     INQSDKKYLFIGIPCFVKSIRLAQKEGMVQNIKFVISLLCGHMKSKDFATSLAWQIGVK
                     PDDLGSVDFRVKEDNKKANDYNIEVEDTCNKKYMHQSSSLFGTNWGLGFFKHHACDFCD
                     DIAGELADATLGDAWLPKYINDSQGTNILIVRNDVLNKLLEQYQEEIVLETANVEDFYE
                     SQAGNFRNRREGLLVRVQNEKKWTPRKRLEIINSDIPLQRRKLYLVRQKLSEQSIKKFQ
                     IAKKIGSIYSFKLLMVPDIFRYKMIEKNLISALKETIRIVAPRKLVKIFKKR"
     gene            4956..6470
                     /gene="wzx"
     CDS             4956..6470
                     /gene="wzx"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="AIG62801.1"
                     /translation="MHRQVFRNVFLNVTTFLFNVVTGLYLTPFLIKYLGLEVYGILPLA
                     LFLTFYIGVITQALTASVNRFFIANLQTNNIKEANVVFNTAFCIIVTYSVLQFALLYYP
                     IQNIDQYFSIPTHSRDDARRLFESVLLSFSLALLTSIFSVSLFALNRLDLIQCVQLIKI
                     IIKFITIILFFEHHCIGIQFVGIAMILSELIALILTICLWRKLTPEINLKLFYFSKKKV
                     GELSKFAMWLIIDQVGYVLFIKMDLLLVNKFFGAKESGRYSIATQFSDLLRSFAGLMAG
                     VLAPVIMILHAKNELERIVTVTKTFVMILSLTIAIPISIICVFSQELIHFWLGQDINIQ
                     TLIWIVTFPLIINLGVLPLFSINVAFKKVKLPSVLNIVLALVGVFVSIILIRNTELGLL
                     AVAAGFGFSLTLKNAVFIPIYAALNLRIDKLTFLTVHVRTILFTFFYVCFLILVKSKLS
                     SNLYGFLAELTVLIIFGFIISIFFYSRDERKYITNTLSDKLKAKVK"
     CDS             6467..7600
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase"
                     /protein_id="AIG62800.1"
                     /translation="MKNKIGILNFQYSDHNYGAVLQAVALENVLKQLGKNAEHINFIPK
                     KKGTKNIKQFIIEFLASIGLKPFIYRVFNKKVIIKNKVEGSEIFEDFRNKYLNRTKAFH
                     SLNDLMELEDSYSSVIVGSDQVWRPKMYSDLTEIEIYFLSFISQKTKKISYAASFGVER
                     WEYDKNDNVTHKAQKYLNKFNSISVREKSGVDICNSVFDIRAEHVLDPTLLIGRDFFDN
                     LIKDKQPLVQKTISYYKLDLCESFLAGIEHLANLTGYETNNIYYKQLNNGSYEYYPVLD
                     WLSMINSSSIIITDSFHCVCFSILFNKSFYCCLNKERGASRLESLLNELGLQDRLISEE
                     ELSRIYTIVDINYSPVEDILNKHRKQSIEFLVSAIDG"
     gene            7605..8489
                     /gene="wfgH"
     CDS             7605..8489
                     /gene="wfgH"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="AIG62799.1"
                     /translation="MSHIIPVVIMTRNEGAYLLKCVQSIINTVTIPYHIYIIDNNSSTL
                     EQEEVLKIIEYKFNDKVNIIRNRTNRWVLGLNKTLIELSKNKEQPYFFLTDGDIDFSRC
                     TAKPCWLSYLVTHMNSNVSLGKIGLSLNWDYISTTKELAAVYEQERSLYNESKKINDLY
                     VSPVDTTAALFRWDWSIEGRPSLYPDHIRYLRPELYSCRTPLDINVEHMGWYKYCAESG
                     DTKNLEEKIVCFTMVGGDVKNEILDKVRFFYKVIYKSLSGPVKKAWYFRRYYYLLKYFL
                     FKGVRRFDGQNCL"
     gene            8518..9633
                     /gene="wzy"
     CDS             8518..9633
                     /gene="wzy"
                     /codon_start=1
                     /transl_table=11
                     /product="GT2|GT2_Glycos_transf_2"
                     /protein_id="AIG62798.1"
                     /translation="MYIFRSEFVLAIFIFHLLFIILALYARKLDNKVLDYIFLILATTL
                     ALIISLFRPGFGVDEPSYLEAFINFKNNADTFPFDYSFKILYEILSNTGIQIEYFNNTV
                     SSLYIILLSITAIVTSSKNNKCFYFLMLLFSFTSLDLMFNAYRQAFAFIFIYNSLFLFL
                     REKKIKGALLAVISFGFHWSAVILILFYFLSRFLKNKYSYLILLLLFPFVLISMFYPLG
                     LMGIGYNVLLFLPLNELFKAHVLAYLEVDNISSASFYILNLFGRLPLAISVLTFYAFIL
                     IFYKKIRMKRIIAMIVLMAVYCLVFMEMAYSFRNYYWVLPFFPIIALDYAESNQAKIHS
                     RMIGIAALHILIALPTFYSSGINSMIFNTGN"
     CDS             9653..10561
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="AIG62797.1"
                     /translation="MCSLKVSALLVSYNPDINRLSEVLNSIHSQVDHLVLVDNGSKNKN
                     DILALIADYEGVYTLLNDNNIGLASAQNVGIDYIIDNNLSDFIVFFDQDSVLQSGFISA
                     LLETYTNLVTQNIKLAAVGPSFIDPVNNKQYPATVYAGPFIKRVDLERKPVEATFIIAS
                     GCFVAIEALKKIGKMKDELFIDYIDVEWCLRARSLGYKIYISPKAVMKHTIGDNRVSIF
                     GRTISVHSPLRRYYLVRNSFYMIRLDYIPIGYKIREIFFNVVRICISLALSDDKKKILH
                     YTITAIKDGVSKKFGPCKKIL"
     gene            10564..11712
                     /gene="epsD"
     CDS             10564..11712
                     /gene="epsD"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|E8RJP5|9.B.97.1.10"
                     /protein_id="AIG62796.1"
                     /translation="MIKIAHIQLLPMLSGVQRVCLDELSRLDKKKYQRYLICKEPGKLS
                     DEATAIGVQCIFLPELQRNISFKMDLIALYKLYRIIKIYKFDIVHTHSSKPGVLGRIAA
                     RLNGVKLILHTVHGFSFPAAQNRFQYYIFWIMEKIGSICGDKLICLHKDDAVIAQEKLF
                     IHKNKISIIENGVDTNKFKKRNNNEIDKLSTYFNIDRNSDVVVGMIGRLWPQKNPMLLL
                     YAAKNIINNNKRVKFLFVGDGELKDSMNDYIIQNKLTQNITICGWCNDVSSYLNIFDIF
                     VLPSLWEGMPLAILEAESSSLPCIVSNIPGNRSIIRHTVDGYLFSLDNPSELESYINIL
                     IEDKERRIILGNNARVKILESYKIENRILKLDELYSSISLKE"
     CDS             11732..12715
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="AIG62795.1"
                     /translation="MRIKILDHMKGAAILAVVFIHSSGYLAVSNIGTFEWYIGIISRQF
                     VNFAVPIFLFISGFLSFSRDIHDTPYYLKKKLLRIIIPYLSWSCFYFFLLFIFNNQSFA
                     VKNIIAQLILGTGIGVGYFVIVLVQFIMLTPLINAITSIKIHIMIICGFTIIGVGINYM
                     SVKIDCLKLFSQFPYSAIPFFVWYPFYHLGFVFNKYNISCSKWSNSSILFWILSFSLTL
                     AISEGIFWGYTGNYSFAVSQLKLSSLVLSLCVCMVVYFYLNHDINYKYLSQLGTMSYGI
                     YLTHMLFVWFYHYLFSYTSVFIRHLVISCGFIFILSTISSVTLVFL"
ORIGIN
        1 tctggtagct gtaaagccag gggcggtagc gtgcattaat acctctatta atcaaactaa
       61 gagccgctaa tttcacagca tgctctgaag taatatggaa taaaaaagtg aagatacttg
      121 ttactggtgg cgcaggattt attggttctg cagtagttcg tcacattata aataatacgc
      181 aggatagtgt tgttaatgtc gataaattaa cgtacgccgg aaacctggaa tcacttgctg
      241 atgtttctga ttctgaacgc tatgtttttg aacatgcgga tatttgcgat gctgctgcaa
      301 tggcgcggat ttttgctcag catcagccgg atgcagtgat gcacctggct gctgaaagcc
      361 atgtggatcg ttcaattaca ggccctgcgg catttattga aaccaatatt gttggtactt
      421 atgtcctttt ggaagcggct cgcaattatt ggtctgctct tgatggcgac aagaaaaata
      481 gcttccgttt tcatcatatt tctactgacg aagtctatgg cgatctgcct catcctgacg
      541 aagtaaataa taaagaacaa ttacccctct ttactgagac gacagcttac gcgcctagta
      601 gtccttattc cgcatcaaaa gcatccagcg atcatttagt ccgtgcgtgg aaacgtacct
      661 atggtttacc gaccattgtg actaactgtt cgaataacta cggtccttat cactttccgg
      721 aaaaattgat tccactagta attcttaatg ctctggaagg taaggcatta cctatttatg
      781 gcaaagggga tcaaattcgt gactggctgt atgttgaaga tcatgcgcgt gcgttatata
      841 tcgtcgtaac cgaaggtaaa gcgggtgaaa cttataacat tggtggacac aacgaaaaga
      901 aaaacatcga tgtagtgctc actatttgtg atttgttgga tgagattgta ccgaaagaga
      961 aatcttaccg tgagcaaatt acttatgttg ccgatcgccc gggacacgat cgccgttatg
     1021 cgattgatgc agagaagatt agccgcgaat tgggctggaa accgcaggaa acgtttgaga
     1081 gcgggattcg taaaacggtg gaatggtacc tgtccaatac aaaatgggtt gataatgtga
     1141 aaagtggtgc ttatcaatcg tggattgaac agaactatga gggccgccag taatgaatat
     1201 tctccttttc ggcaaaacag ggcaggtcgg ttgggaacta cagcgtgctc tggcaccttt
     1261 gggtaatttg attgctcttg atgttcactc cactgattat tgtggtgatt ttagtaatcc
     1321 tgaaggtgta gctgaaaccg tcaaaaaaat tcgccctgat gttattgtta atgctgcggc
     1381 tcataccgca gtagataagg ctgagtcaga acccgaattt gcacaattac tcaatgcgac
     1441 cagcgttgaa tcaattgcaa aagcggctaa tgaagttggg gcctgggtaa ttcattactc
     1501 aactgactac gtatttccgg ggaccggtga aataccatgg caggaggcgg atgcaaccgc
     1561 accgctaaat gtttacggtg aaaccaagtt agctggagaa aaagcattac aagagcattg
     1621 tgcgaagcac ctaattttcc gtaccagctg ggtctatgca ggtaaaggaa ataacttcgc
     1681 caaaacgatg ttgcgtctgg caaaagagcg tgaagaatta gccgttatta atgatcagtt
     1741 tggtgcgcca acaggtgctg aactgctggc tgattgtacg gcacatgcaa ttcgtgtggc
     1801 actgaataaa ccagaagtcg caggcttgta ccatctggta gccagtggta ccacaacctg
     1861 gcacgattat gctgcgctgg tttttgaaga ggcgcgcaaa gcaggcattc cccttgcact
     1921 caacaagctc aacgcagtat caacaacagc ctatcctaca ccagctcgtc gtccgcataa
     1981 ctctcgcctt aatacagaaa aatttcagca gaactttgcg cttgttttgc ctgactggca
     2041 ggttggtgtg aaacgcatgc tcaacgaatt atttacgact acagcaattt aatagttttt
     2101 gtatcttgtt cgtgatggtg gaacaagata aattaaaagg aatgatggaa tgaaaacgcg
     2161 taaaggtatt attttagcgg gtggttctgg tacacgtctt tatcctgtga ctatggctgt
     2221 cagtaaacag ctattaccta tttatgataa accgatgatc tattatccgc tctctacact
     2281 gatgttggcg ggtattcgcg atattctaat tataagtacg ccacaggata ctcctcgttt
     2341 tcaacaactg ttgggtgacg ggagccagtg gggcctgaat cttcagtaca aagtgcaacc
     2401 aagtccagat ggtcttgcgc aagcatttat catcggtgaa gaatttatcg gtggtgatga
     2461 ttgtgctttg gttcttggtg ataatatttt ttacggtcac gatctgccga agttaatgga
     2521 tgccgctgtt aacaaagaaa gtggtgcaac ggtgtttgcc tatcacgtta atgatcctga
     2581 acgctatggt gtcgttgagt ttgataaaaa cggtacggca attagcctgg aagaaaaacc
     2641 gctacagcca aaaagtaatt atgcggtaac tgggctttat ttctatgata acgacgttgt
     2701 agaaatggcg aaaaatctta agccttctgc ccgcggtgaa ctggaaatta ccgatattaa
     2761 ccgtatctac atggaacaag ggcgtttatc tgttgccatg atgggacgtg gatatgcatg
     2821 gttagacacg gggacacatc aaagcctgat tgaggcaagc aacttcattg ctacaattga
     2881 agagcgtcaa gggttgaaag tttcctgtcc agaagaaatt gcttaccgta aagggtttat
     2941 caatgctgag caggtgaaaa tattagctga accgttgaag aaaaatgctt atggtcagta
     3001 cctgctaaaa atgattaaag gttattaata aaatgaacgt aattaaaaca gagattccag
     3061 acgtattaat tttcgaaccg aaagtttttg gtgacgagcg cggtttcttt ttcgagagct
     3121 ttaaccagaa ggtttttgag gaagctgtag gccgcaaagt tgaatttgtc caggataacc
     3181 attcgaagtc ttgtaaaggt gttttacgcg gactgcatta tcagttagaa ccttatgcac
     3241 aaggaaagtt ggtgcgctgc gttgttggtg aagtttttga cgtagctgtt gatattcgta
     3301 aatcgtcgcc tacctttggt aaatgggttg gggtgaattt atctgctgag aataagcggc
     3361 aattgtggat ccctgaggga tttgcgcatg ggtttttggt gctgagcgac gaggcagaat
     3421 ttgtttataa gacaaacaat tattattatc aaaagcatga aagaagcatt ctttggaacg
     3481 ataaaattct caacgtagaa tggccattta cttctaactt aattctttct tcaaaagata
     3541 tgagcgcgtc attattcact gttgcagaaa aattttaatt ggttttggaa ataaaatgtc
     3601 tatacaaaat gtaatagata atggttactg cgtcggatgt ggtgcttgta aattggctct
     3661 tggagagtca gtggaaataa aaatgtatga agatggcttt tataatgctt cgataaaacc
     3721 taatgcaaat attgaaattg ccgagaaaat ttgtccattt tctgatagtt caacaaatga
     3781 aactatctta ggcgcatcac tttatggaga taaaaaattt gacaagcgtg taggatattt
     3841 tgacgacatt tatattgggt atgtagttga taatcaggac cgactaactt ccagttcagg
     3901 tggacttact acctggtttg ctaaaaagtt attaataaat aaagaggttg atgcggtaat
     3961 tcatgtaggg catgggacgc atatgtttga ctacattatt actgagaata ttttagatct
     4021 tgatttacag aataataaaa aatccagata ttatcctgta agtttttctg aattgataaa
     4081 taaaataaac caatcagata aaaaatacct tttcattggc attccatgtt ttgttaaatc
     4141 aataagatta gcgcagaaag aaggaatggt tcagaatatt aaatttgtga tttcattact
     4201 ctgcggacat atgaagtcca aagatttcgc tacgagtctt gcgtggcaga ttggcgttaa
     4261 acctgatgat ctgggtagtg tagactttcg tgtcaaggag gataataaaa aagcaaatga
     4321 ttataatata gaagttgaag atacttgtaa taagaaatat atgcatcaaa gctcttcgtt
     4381 gtttggcact aattgggggc taggcttttt caaacatcat gcatgtgatt tttgtgatga
     4441 cattgcaggt gaattggcgg atgcaacttt aggtgatgct tggttaccga agtatataaa
     4501 tgattcacaa ggtactaata tacttattgt aagaaacgat gttttaaata aattacttga
     4561 acaatatcaa gaagaaatag tgcttgaaac tgcgaatgtc gaggattttt atgaaagtca
     4621 agctgggaat tttcgtaatc gacgtgaagg gttacttgtt cgtgtccaaa atgaaaaaaa
     4681 atggactcct agaaaaaggt tggaaattat taactccgat attccattac aacgtcgtaa
     4741 attgtattta gttcgtcaga agttaagtga acaaagtata aagaaatttc aaatagctaa
     4801 aaagataggt tcaatatatt cattcaaatt gttaatggtt cctgatattt ttagatataa
     4861 aatgattgag aagaatttaa tatcggcgtt aaaagaaact ataagaatag tggcgccaag
     4921 aaaacttgta aaaatattca aaaaacggta gctttatgca taggcaagtt tttcgaaacg
     4981 tatttctaaa tgtgacgaca tttttattta atgtagttac tggattatat ttaactccat
     5041 ttttgattaa atatttaggg ttggaagtgt atggtatact tcctttagca ttgtttttaa
     5101 ccttttatat tggcgtgata actcaggccc ttacggcatc agtaaatcga ttttttattg
     5161 caaatttaca aactaataac attaaagagg ctaatgttgt ttttaacact gctttttgta
     5221 tcatagttac atattcggta ttgcagtttg ctctcctata ctatcctata caaaatatag
     5281 accagtattt ttctataccg acgcatagca gagatgatgc ccggagattg tttgaatcag
     5341 ttttactcag tttctctctt gcgcttttaa catccatttt tagtgtttcg ctttttgcat
     5401 taaacaggtt ggatttgatt caatgcgtcc agttaatcaa aattattata aagttcatta
     5461 ccataatatt attctttgaa catcattgca taggtatcca gtttgtagga atagcaatga
     5521 ttctgtcaga gttaatagct ttaattttga cgatttgttt atggagaaaa ttgacaccgg
     5581 aaataaattt aaaactattt tacttcagta aaaagaaagt aggagagctt tcaaagtttg
     5641 caatgtggtt gattattgat caggttggat atgttctttt tattaaaatg gatttattat
     5701 tggtaaacaa attctttggg gcgaaggaat ctgggcgcta ttcaattgca acgcaattta
     5761 gtgatttact aagatcattt gcagggctta tggcgggagt tcttgcacct gtaataatga
     5821 tactacacgc caagaatgaa ttagaaagaa ttgtaaccgt taccaaaacg tttgtgatga
     5881 ttctatcgct gacaatagcg ataccaattt ctataatctg tgtattttct caagagctga
     5941 ttcatttctg gctaggacaa gatataaata tccaaacttt aatttggatt gtgacttttc
     6001 cattaataat taatttgggc gtcttaccat tattttctat aaatgtagcg tttaaaaaag
     6061 ttaaactacc tagtgttcta aatatcgtat tggcactagt tggcgtattt gtctcaatta
     6121 tacttattcg aaatactgaa ctgggtttac ttgcagtagc ggctgggttt ggtttttccc
     6181 tgaccctcaa aaatgcggta tttataccta tttacgcggc actaaattta agaatagata
     6241 aattaacatt tctaactgta catgtaagaa ctattttatt tacatttttt tatgtatgtt
     6301 ttctgatttt agttaagtca aaactgtcta gtaatcttta cgggttttta gctgaattaa
     6361 cagtgcttat tatatttggc tttataatta gtatcttttt ttattctcgg gatgaaagga
     6421 agtatattac taatacactt tcagataaat taaaagcgaa ggtaaaatga aaaataaaat
     6481 tggtattcta aattttcaat attcggatca taattatggt gcagtcttac aagctgttgc
     6541 actagaaaat gttttaaaac agctagggaa aaatgctgaa catattaatt ttattccaaa
     6601 gaagaaaggt accaaaaata tcaaacaatt tataattgaa tttttagctt ctattggttt
     6661 aaaacctttc atttatcggg tatttaacaa aaaagttatt ataaaaaata aagttgaagg
     6721 atctgagatt tttgaggatt tcaggaataa atatttgaac agaacaaaag cgtttcattc
     6781 cctcaatgac cttatggaat tagaggatag ttacagttct gttatcgttg gaagtgatca
     6841 agtctggcgc cccaaaatgt actcagacct tacagaaatt gaaatttatt tcctttcttt
     6901 tatttcacag aaaacaaaaa aaatttctta tgcagcaagt tttggtgttg agcgatggga
     6961 atatgacaaa aatgataatg tgacacataa agcacaaaaa tatctgaaca aatttaattc
     7021 tatttctgtt agagaaaaat caggagtaga catttgtaat agtgtatttg atattagagc
     7081 tgagcatgtt ctggatccaa cactattgat cggacgtgat tttttcgaca atcttattaa
     7141 agacaaacaa ccattagttc aaaagacaat aagttattat aagttagatc tttgtgagtc
     7201 ttttttagcg ggaatagagc atcttgctaa tctaaccggc tatgagacta ataatattta
     7261 ctacaaacaa ctgaataatg ggagttatga atattatcca gttttagatt ggctaagcat
     7321 gattaattca tcttcgataa taataacaga ctcttttcat tgtgtatgct tctcaatttt
     7381 atttaataaa agcttctatt gttgtcttaa taaagaaaga ggagcatcga ggcttgaaag
     7441 tttattgaat gaactggggt tgcaagacag attaatctct gaagaagaac tatcgagaat
     7501 ttatacaata gttgatatca attattctcc tgtagaagat attttgaata agcacaggaa
     7561 acagtcaata gaatttttag tttcagcaat tgatggataa ttaaatgagt catataattc
     7621 cagtagttat tatgacacgg aatgaaggtg cttacctttt gaaatgtgtc caatcaatca
     7681 ttaatactgt tactataccc tatcatatat atattattga taacaattct tcaacattgg
     7741 agcaagaaga agttctaaaa attattgaat ataagtttaa tgataaagta aatattatta
     7801 gaaatcgtac taacaggtgg gttctgggtt taaataagac acttattgaa ttgagtaaaa
     7861 ataaagaaca accctatttc tttttaactg atggtgatat tgacttttca agatgcaccg
     7921 ctaagccatg ttggttatca tatttggtta cccatatgaa ttctaatgta tctctgggga
     7981 aaattggctt atctctcaat tgggattaca tttcaactac aaaagaattg gcggctgttt
     8041 atgaacagga gagaagtcta tataatgaaa gtaaaaaaat aaatgattta tatgtgtctc
     8101 ctgttgatac aacagcggca ttatttcgat gggattggtc tatagaagga agaccaagct
     8161 tatatccaga ccatattaga taccttagac cagaacttta ttcatgtcga acaccattag
     8221 atattaatgt agagcatatg ggatggtata aatattgtgc agaatctgga gatacaaaaa
     8281 atttagagga aaaaatagtc tgcttcacta tggttggtgg tgatgttaaa aacgaaattt
     8341 tagataaggt tcgttttttc tataaggtta tatataaatc attgtctggt ccagtaaaaa
     8401 aagcatggta tttcaggaga tattattatc ttttgaaata ttttcttttt aagggcgttc
     8461 gcagattcga tggtcaaaac tgtctttgaa atgtaatgta attattggag tgtaactttg
     8521 tatatatttc gaagtgagtt tgtactagcg atatttattt ttcatttgtt attcataata
     8581 ttggccttat atgcacgaaa acttgataat aaggtattag attatatatt tcttatattg
     8641 gcaactacat tagccttaat tatttcactc tttagacctg gctttggagt tgatgaacct
     8701 tcttatcttg aggcatttat taatttcaaa aataatgctg acacatttcc attcgattat
     8761 tcatttaaaa tattatatga aatattatct aatacgggca tacaaattga gtatttcaat
     8821 aacacagtca gctctttata tattatttta cttagtataa cggcgatagt aacatcgagt
     8881 aaaaataata agtgttttta tttcttgatg ttattgttca gttttacttc attagatcta
     8941 atgtttaatg cttacaggca agcatttgca tttatattta tttataactc tttatttctt
     9001 tttttgagag aaaagaaaat caagggggct ttattagctg taatctcttt tgggtttcat
     9061 tggtcagcag ttatcctgat cttattttat tttttatcga ggtttcttaa gaataaatat
     9121 tcttacctta ttttactttt attatttcca tttgtattaa tctcgatgtt ttatccatta
     9181 ggtttgatgg ggataggata taatgtgttg ttatttcttc ctttgaatga attattcaaa
     9241 gctcatgtgt tagcgtattt agaagttgat aacatttcta gcgcgtcgtt ttatatactt
     9301 aatctctttg gtcgactacc attggctata tcggtattaa ctttttatgc ttttatactg
     9361 attttttaca aaaaaataag aatgaagcgt ataatagcaa tgattgtctt gatggctgtt
     9421 tattgtctag tgtttatgga gatggcatat agttttagaa attattattg ggttctacca
     9481 tttttcccta ttattgcttt agattacgca gaatctaatc aagcgaaaat tcattcaaga
     9541 atgataggca ttgctgctct tcatatcttg atagcattgc ctacttttta cagttcaggt
     9601 ataaactcta tgattttcaa tactggcaat tagtattgaa tatggaaaaa aaatgtgttc
     9661 attaaaagtt agcgctttac tcgttagtta caatccagat attaatagat taagtgaggt
     9721 tcttaatagt attcattctc aagtagatca tctagtttta gttgataatg gttcaaaaaa
     9781 taaaaatgat atacttgctc tcattgccga ttatgagggg gtttatacac tgcttaatga
     9841 taataatata ggtttagcct ctgctcaaaa tgttggtatt gattatatta ttgacaataa
     9901 cctttccgat tttatcgtgt tcttcgacca agatagtgta ttgcagtcag gttttattag
     9961 tgcgctactc gaaacgtata ctaatttagt aactcagaat attaaattag cagcagtagg
    10021 accatcattt attgatcctg taaataataa acaatatcct gcgactgttt atgcaggacc
    10081 atttattaaa cgagtagatc ttgaaagaaa acctgtagaa gctaccttca taattgcttc
    10141 tggatgcttt gttgccattg aggcattgaa aaaaattggg aaaatgaagg atgaattgtt
    10201 tatagactat atcgatgttg aatggtgtct cagagctagg agtttaggat acaaaatata
    10261 tatttcacct aaagctgtaa tgaaacatac tattggagat aatcgagtat ctatctttgg
    10321 gcgaacaata tctgttcatt caccattacg tcgatattat cttgtaagga atagctttta
    10381 tatgattaga ttggattata taccaattgg atataaaatt agagaaatat tttttaatgt
    10441 agttcgtatt tgtatcagct tagctctatc tgatgataaa aaaaagatac ttcattatac
    10501 aataactgca attaaagatg gtgttagtaa aaaattcgga ccttgtaaaa aaatactcta
    10561 attatgatca aaatagctca tattcaatta ttgccaatgc ttagtggtgt tcagagagtg
    10621 tgtttggatg agctctcaag attggacaaa aaaaaatatc aaagatactt aatttgtaaa
    10681 gaaccaggta aactcagtga tgaggctact gctattggcg ttcaatgtat ctttcttccc
    10741 gagcttcagc gcaatatcag ctttaagatg gacttaatag cgttgtacaa attatatcgg
    10801 ataataaaga tatataaatt cgatattgtt catacacact cttctaagcc aggtgtttta
    10861 gggcgtattg cagccaggct gaatggtgtt aaactgatac ttcacactgt gcacggtttt
    10921 tcttttcctg ctgcacaaaa taggttccaa tattatatat tttggatcat ggaaaaaatt
    10981 ggctcaatat gtggagataa attaatatgt ctgcataaag atgatgcagt gattgctcag
    11041 gaaaaattat ttattcataa gaataaaatt tccataattg aaaatggcgt cgacactaac
    11101 aaattcaaga agagaaataa taatgaaata gataaattat ctacatattt taacatagat
    11161 agaaattcgg atgttgtggt cgggatgata ggtagactgt ggccacaaaa aaaccccatg
    11221 cttttattat atgcagcaaa aaatataatc aacaataata aaagagtaaa attcttgttt
    11281 gtcggcgatg gtgagttaaa agactcaatg aacgattata ttattcaaaa taaacttacc
    11341 cagaatatta ccatttgtgg ctggtgtaat gatgttagtt cttatttgaa tatatttgat
    11401 atttttgtat taccatcttt atgggagggt atgcctttgg caattttaga agccgaaagt
    11461 agttcgttgc cctgtattgt ttcaaatatc ccgggaaaca ggagtataat caggcataca
    11521 gttgatggct atctcttttc attagataac ccatctgaac ttgaatctta cattaatata
    11581 ttaattgagg ataaagaaag acgtattatc ttgggtaata acgccagagt caaaattctt
    11641 gaaagctata aaatagaaaa taggattttg aaattagatg aactttatag ttcaatttct
    11701 ttaaaagaat agcgttgtat gttgagggga tatgcggata aaaatactcg accatatgaa
    11761 aggtgctgct attttagctg tggtatttat tcattcttca ggttatcttg ctgtttctaa
    11821 tataggaaca tttgaatggt acatcggtat aatatcgcgg caatttgtta attttgcagt
    11881 gcctattttt ttatttatat ctggtttttt atctttttct cgagatatac atgatacacc
    11941 atactatctc aagaaaaaat tattaagaat tattatacct tatctctcat ggtcttgttt
    12001 ttacttcttt cttttattca tatttaataa ccaaagtttc gctgtcaaaa atattatagc
    12061 tcagttgatt cttggtacgg gaataggtgt gggatatttt gtgatcgttt tagtgcaatt
    12121 tattatgctg acaccgttaa ttaatgcgat tacatctata aaaatacata ttatgattat
    12181 atgtggtttt actatcatag gtgtggggat taattatatg tctgtgaaga ttgattgctt
    12241 gaaactgttt tcacagtttc cttattccgc cattcctttt tttgtatggt atccttttta
    12301 tcatttgggc tttgttttca ataagtataa tatatcttgt tcaaagtggt ctaatagttc
    12361 catcttattt tggattttat ctttttcact tacattagcc atttctgaag ggatattttg
    12421 gggttatact gggaactatt catttgctgt gtctcaactt aaactatcat ctttggttct
    12481 ctctttatgc gtttgtatgg ttgtttattt ttatctaaac catgatataa attataaata
    12541 cctttctcaa ttagggacaa tgtcctacgg gatttatctt actcatatgt tgtttgtttg
    12601 gttctatcat tatctctttt cgtatacgag tgtgtttata aggcatttag ttatctcgtg
    12661 tggctttatc tttatattaa gtacaatatc atcagttacc ttagtttttt tataagaaaa
    12721 attttgggga gatattccgt atatattgta gggtaggtga ttgcttgata tttatactgt
    12781 tttaagaaga agcctaattt atctcaatac tgggcatttc actagagctg tattagtata
    12841 tttgtcacat tctcatccgt atttctaatg tatttttcag agcaggagac ttaaatgtca
    12901 aagcaacaga tcggcgtcgt cggtatggca gtg
//
