LOCUS       KY574598               15691 bp    DNA     linear   BCT 19-SEP-2017
DEFINITION  Escherichia albertii strain T20150072 O-antigen biosynthesis gene
            cluster, partial sequence.
ACCESSION   KY574598
VERSION     KY574598.1
KEYWORDS    .
SOURCE      Escherichia albertii
  ORGANISM  Escherichia albertii
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15691)
  AUTHORS   Naumenko,O.I., Zheng,H., Senchenkova,S.N., Wang,H., Li,Q.,
            Shashkov,A.S., Wang,J., Knirel,Y.A. and Xiong,Y.
  TITLE     Structures and gene clusters of the O-antigens of Escherichia
            albertii O3, O4, O6, and O7
  JOURNAL   Carbohydr. Res. 449, 17-22 (2017)
   PUBMED   28672166
REFERENCE   2  (bases 1 to 15691)
  AUTHORS   Zheng,H. and Xiong,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2017) State Key Laboratory for Infectious Disease
            Prevention and Control, National Institute for Communicable Disease
            Control and Prevention, Chinese Center for Disease Control and
            Prevention, P.O.Box 5, Changping, Beijing 102206, P.R. China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: SOAPdenovo v. 1.04
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15691
                     /organism="Escherichia albertii"
                     /mol_type="genomic DNA"
                     /strain="T20150072"
                     /serotype="O6"
                     /db_xref="taxon:208962"
     misc_feature    <1..>15691
                     /note="O-antigen biosynthesis gene cluster"
     CDS             1..894
                     /codon_start=1
                     /transl_table=11
                     /product="UTP-glucose-1-phosphate uridylyltransferase GalF"
                     /protein_id="ARO73527.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEIV
                     AAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNVRQ
                     GEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRSQVL
                     AKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLSADIW
                     PELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKYGLRNL
                     KEGAKFRKGIEKLLNE"
     CDS             1429..2490
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="ARO73528.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEYKLSDILLLTICAVISGAEGWE
                     DIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCINPAKFHECFINWMRDCHCSDDKD
                     VIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLNMMD
                     IKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPKHDS
                     YAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTV
                     RYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDGCKIRRGNAAELFSGIRHIA
                     INILTNDKVFKHG"
     CDS             2677..3840
                     /codon_start=1
                     /transl_table=11
                     /product="GT4"
                     /protein_id="ARO73529.1"
                     /translation="MKSTYSKIKIAHVQLMPLLSGVQRVSLQEFELLPNEQFDINLICK
                     ESGPLTDYLDDSVRAFFVPTLCRNISPIEDMKSLISLYKLLKKEKYDIVHTHSSKTGIL
                     GRIAARLAGVPCVVHTVHGFAFESTKRKSVKLVYKWLEIFAAKCTTRLICLHNEDKEIC
                     IKELYVDPMKISVIPNGVDLEKFAPAINKGDLKEKILGLKRNSFVFTMVGRLWPQKNPL
                     YFAEAAKYIIENNLIPDSVFVIVGDGELMNDLKYNYQTDMNLKKRLLLLGWRNDIPNIL
                     KASDVFVLPSLWEGMPLAILEAQSTGLPCIVSNINGNNCLVKNEFDGFLIELNDIDSFI
                     NALIRVTDDKVLNIMSKNCRNKIVNEFNIVKRVDKIKNLYIDMVVNK"
     CDS             4691..6205
                     /codon_start=1
                     /transl_table=11
                     /product="Wzx"
                     /protein_id="ARO73530.1"
                     /translation="MVKMFDSMHVRVAVNFASKITFALISIITIPVIKSKLGVNAYGVL
                     TFFLSIQVVILLLEGGMSATMARGLLNKKYKIYNISKAEYQSVYFIFFILVSLFVFSIF
                     IVFNHNISTLIYNLDDRKVYSFISADHVILLAGAMIALQFYIIYYEALFVAYEKQVSYG
                     IHIIVFNVIKTIISLFLLIVFNLGLDVYFLVHVFCTFILIILLHIKAIKNGLLEITTLR
                     VKKIYTKISFMKEEFHFSKNILCVSILSAIAFQADRLFVTKYMGISAVGMYGIAYTLST
                     VPTLFTSSLYNVIYPRLIILIREQSSSAISFFELVSKLIYIIIIPTCVIISLNSTQILN
                     IWIGKHEGIISLLLSILIIATLFQGLQVIPFALQMAQERLKFTVFMNAIFVPSLILAYY
                     LVSIHKILLYISIVWSVYNIITFFSLTIYLYAKNKMYTRLLILYFNICLCAVVTVVICS
                     IFKNQESQIFLSLVNISLQFFMSMSLCIILLFWKTIYGALRCVCCR"
     CDS             6187..7356
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase 2 family protein"
                     /protein_id="ARO73531.1"
                     /translation="MCLLSVVIATHNRAGYAKKSIESILSIDVEEFQLIIHDTSDNNVL
                     EEFCAEIQDARLKYIHCNELLSMTENFNRALEYVQGEYTIMIGDDDTILSTAIKYCRYA
                     KEKKIDVISQKISAEYCWPDFRSKISGDFNASSLIVNKFTSNQVTYEGRKQYYEAVKNC
                     FQGTASLPKLYHGIVKTSLLLKIKENTGTYFHGVSPDISIALSLAYNCERYIEVDYPIT
                     LPGSSGGSNAGRSAMRTHVGKLDDDPHMKRFKSYVWPNLLPRYFSVETVWAQAGLVTIQ
                     KIDNNKGFNFIKFYALCLIKHRPYKKFIIDEIKKYKKVKGGFNCIIYAKIIYHMSYFYI
                     NGISNLSKKIIKYVFKKLKSQEKQKNIKANDIFEARMIIEKELSKETFE"
     CDS             7353..8438
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy"
                     /protein_id="ARO73532.1"
                     /translation="MIIFSTFLLFYFFIFLSLLPVLIKEGARGNGKYFYFFDLYIYLVL
                     ASLIIYIICTRDIFSDSDTERYLSILEYYRINGFYLFDKNYIFAVYTYLYSLYIDGYYM
                     YMIIPLITLLYSYCKLVKPLKYPVYSFSLVLIATFPVFWQLSASAFKQCIAISFILLAL
                     VHIIQTKYKQAVLLSLIASSFHFTALTFFIFSLFFYIRKERVSLNLFFIIWFFSVIFSI
                     SGLNSYIPSIIPFYDLVGESKVNIYLESTNNATGFRVDFFLFSLAPVLIIFINRTLVIK
                     DHYIKMIALSYLYFNSIYNIMSFIPFSDRVAAYSWCLSPIILLWSALKCRSSIYMLSSI
                     GIALLNPLIFMCYNSMFIFLK"
     CDS             8445..9458
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|Q6MMD5|9.B.18.2.1"
                     /protein_id="ARO73533.1"
                     /translation="MFNGKKILITGGTGSFGNAVLRRFLETDIQEVIIFSRDEKKQDEM
                     RTLYRNDKMKFIIGDIRDYTSVLAATSDVDYIFHAAALKQVPSCEFYPWEAVKTNIIGT
                     ENLLNAAKQNKVKKTICLSTDKAVYPINAMGISKAMMEKVMTAKARTFNNEDSVVCGTR
                     YGNVMASRGSVIPLFIQQIQTNQPLTITDPEMTRFMMTLDDAVDLVLFAFNNGQNGDIF
                     VQKAPAATIAVLAKALVELMGVPNHPIKVVGSRHGEKIYETLLSREEMAKAIDHGDYYQ
                     IQPDNRDLNYDKYLSEGSKKIELFDDYNSHNTYRLNVDEMKKLLLKLNIIQNVLNS"
     CDS             9471..10340
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2-acetamido-2,6-dideoxy-L-lyxo-4-hexulose"
                     /protein_id="ARO73534.1"
                     /translation="MQKILILGVSGMLGHTLFRFLSSQQDLLVTGTVRHITQEIKLVSS
                     KNIVLMRDVFNKEKLENIINSNDVIINCIGAIKQKYDNKNTDYILLNSYFPHLLNEICI
                     ESNKRLIHFSTDCIFNGEKGNYDDGSLSNVIDMYGKSKYLGEVYGDKTLTLRTSLIGHE
                     LKSSYSLIDWFLNSNSTVNGYTKAIFSGLPTIEIAHFLYEHVLQSQLHGIYNLSAAPIS
                     KFDLLTLVNEVYKLNKTIVPKSDFVIDRSLDSHRLRSLTKYTPPDWNTLVVKMYLDYLS
                     LGALLNER"
     CDS             10330..10797
                     /codon_start=1
                     /transl_table=11
                     /product="wclT"
                     /protein_id="ARO73535.1"
                     /translation="MKDNLSFSKLISEAEINSRNRSHLNLHESFSDPIQKTLICCCSNT
                     YIPPHYHKFAHQSELFIRLKGCFSLLIFSVDGVLTRRITIDEENPLYEIKPGVIHTVVA
                     LDRHNILLEIKRGPYVESEAKNFPDWVTLENSDNQLDLERLRTLKVGDKLS"
     CDS             10832..11629
                     /codon_start=1
                     /transl_table=11
                     /product="GT2|GT2_Glycos_transf_2"
                     /protein_id="ARO73536.1"
                     /translation="MLPLISIITPTYNRAYTLDRLYNSLVTQDSEKFEWIIIDDGSADE
                     TRKKCLGYINDSIFKIQYYKQNNSGKPAAINLGVTKAVGDYIFIVDSDDALTLNAIGTI
                     YDCIVKYTVASDFEFSGLGFRKSFFDGKMLGKHIERNTNSPMLLHSTDCNNLFKVDLAY
                     CFKRELMIKYPFPKFKDEKFVPELFIWNKITDVAKIIFFPNVVVYYCEYLADGLTNNFK
                     KQLRHNPIGFAVYYKDQFHREYSIWGKIKMAIRYIQCIIYKFI"
     CDS             11632..12750
                     /codon_start=1
                     /transl_table=11
                     /product="GT4"
                     /protein_id="ARO73537.1"
                     /translation="MQGSSIHLISCYDFYMKILHVVPQLKHGGVETAVFNSIERIRKSG
                     YDFNVLTIERGNCFNNSYVKVEHCLNSSVYNPVCFYKFIRFIHKEKPDLIVYSLWKSAL
                     LGIALCLLKPFLKSKPRTALIIHNTRFAHFVDYIITKLSLKITDSIYFDSEKSKAYFLN
                     RDYATGEVISFIDKKMVAKKFNNINGEIKFVFIGRLHSQKGVDRALKYIHLFKKQHEEI
                     RFDIYGPDEGELNNIINKVNKLGLQDKVVYKGVVDNNKVNDILTQYDFYLQFSHFEGMA
                     MSVVDAMQVGVIPIVNNVGEIPNYVKNNINGIILDDKKLGDENYLNLNISCLLNIIKND
                     GGYFLYNNAIKTFQDNNTYVDDYIKKIINANN"
     CDS             12870..14276
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="ARO73538.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIAE
                     NPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGNTF
                     FKDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILSKIAAVA
                     EDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQTFTEW
                     NNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEPLSLIT
                     ESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYAQGFSQL
                     RAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPYFKQIADD
                     YQQALRDVVAYAVQNGIPVPTFSAAVAYYDSYRAAVLPANLIQAQRDYFGAHTYKRIDK
                     EGVFHTEWLD"
     CDS             14525..15691
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="ARO73539.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISPI
                     VDKEIQQFLQSDKIHFNATLDINEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIKDV
                     VEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVIGER
                     SERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYAESLG
                     LNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAIVDANR
                     TRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVIIYEPMMK
                     EDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
ORIGIN
        1 atgacgaatt taaaagcagt tattcctgta gcgggtcttg ggatgcatat gttgcctgcc
       61 actaaggcga ttcccaaaga gatgctaccg atcgtcgaca agcctatgat tcagtacatt
      121 gttgacgaga ttgtggctgc agggatcaaa gaaatcctcc tggtaactca tgcgtccaag
      181 aacgcggtcg aaaaccactt cgacacctct tatgaattag aatctctcct tgagcagcgc
      241 gtgaagcgtc aactgcttgc ggaagtgcag tccatctgcc caccgggcgt gaccattatg
      301 aacgtgcgtc agggcgaacc tttaggttta ggccactcca ttttgtgtgc acgacccgcc
      361 attggtgata acccatttgt cgtggtgctg ccagacgttg tgatcgacga cgcgagtgcc
      421 gatccgctgc gttacaacct tgctgccatg attgcgcgct tcaacgaaac gggccgtagc
      481 caggtgctgg caaaacgtat gccgggtgac ctctctgaat actcggtcat ccagaccaaa
      541 gaaccactgg accgtgaagg taaagtcagc cgcattgttg aatttatcga aaaaccggat
      601 cagccgcaga cgctggactc agacatcatg gccgttggtc gctatgtgct ttctgccgat
      661 atttggccgg aacttgaacg cactcagcct ggcgcatggg ggcgtattca gctgactgat
      721 gccattgccg aactggcgaa aaaacagtcc gttgatgcca tgctgatgac tggagacagt
      781 tacgactgcg gtaaaaaaat gggctatatg caggcgttcg tgaagtatgg actacgcaac
      841 ctgaaagaag gggcgaagtt ccgtaaaggg attgagaagc tgttaaacga ataatgaaaa
      901 tctgaccgaa tgtaacggtt gataagaaaa ttataatggc agtgaagatt cgttgcgaaa
      961 gtaatttgtt gcgaatattc ctgccgttat tttatataaa caatcagaat aacaacgagt
     1021 tagcaataag attttagtca aagttttcca ggattttcct tgtttccaga gcggattggt
     1081 aagacaatta gcgtttgaat ttttcaggtt tagcgcgagt gggtaacgct cgtcacatcg
     1141 tagacatgca cgcagtgctc tggtagctgt aaagccaggg gcggtagcgt atctagggga
     1201 agatcatcgt gtgcggtaag cacaaaaatg caaaaaggaa cgttttgcat gtaataagca
     1261 taagcttatg aagagatcag ggaacaggga aagatcacgt aacgccggtt ttttgtacta
     1321 aataattcgc actaaatgtt caataattga gacattcctt attgtctaaa actgtttttc
     1381 acagcttata tatgatcaaa tactccttac ataaataagg agaacaaaat ggaacttaaa
     1441 aaactgatgg aacatatttc tattataccc gattacagac aagcctggaa agtagaatat
     1501 aaattgtcag atattctact attgactatt tgtgccgtca tttctggtgc agaaggttgg
     1561 gaagatatag aggattttgg tgaaacgcat ctcgattttt tgaagcaata tggtgatttt
     1621 gaaaatggta ttcctgtcca tgataccatt gccagagttg tatcctgtat taatcctgca
     1681 aaatttcacg agtgctttat taactggatg cgtgattgcc attgttcaga tgataaagac
     1741 gtcattgcaa ttgatgggaa aacgctccgg cactcttatg ataagagtcg tcgcagggga
     1801 gcgattcatg tcattagtgc gttctcaaca atgcacagtc tggttctcgg acagatcaag
     1861 acggatgaaa aatctaatga gattacagct attcctgaac ttcttaacat gatggatatt
     1921 aaaggaaaaa tcatcacaac ggatgcgatg gggtgccaga aagatattgc agaaaagata
     1981 caaaaacagg gaggtgatta tttattcgct gtaaagggaa atcaggggcg actaaataaa
     2041 gctttcgagg aaaaatttcc gctgaaagaa ttaaataatc caaagcatga cagttacgca
     2101 attagtgaaa agagtcacgg cagagaagaa atccgtcttc atattgtttg cgatgttcct
     2161 gatgaactta ttgatttcac gtttgaatgg aaaggactga aaaaattatg cgtggcagtc
     2221 tcctttcggt caataatagc agaacaaaag aaagagccag aaatgacggt cagatattat
     2281 atcagttctg ctgatttaac cgcagagaag ttcgccacag caatccgaaa ccactggcac
     2341 gtggagaata agttgcactg gcgtctggat gtggtaatga atgaagacgg ctgcaaaata
     2401 agaagaggaa acgcagcaga attattttca gggatacgac acatcgctat taatattttg
     2461 acgaatgata aggtattcaa gcacggttaa gacgttcgat gaagacagcg actacctgcg
     2521 tcagtctttg tgaggagcgg actctagtaa tccaactttg ttgttaggtt ttactcgcgg
     2581 aataatcatg ggcgaatttt attaaaaatg tatattttcc taattaaaaa catgtttaaa
     2641 aaagaaacac aactttcaag ctaggttact tatgatatga agtcaacata ttctaaaatt
     2701 aaaattgcac atgttcaatt gatgccacta ctgagtggtg ttcagcgagt atcgttacaa
     2761 gaattcgaat tattaccaaa tgaacagttc gacattaatt taatttgcaa agagagtgga
     2821 ccattaactg attatttaga tgatagtgta agggcttttt ttgtcccgac attatgtaga
     2881 aatatatcgc ccatagagga tatgaaatca ttaatatctt tatataaatt attgaaaaaa
     2941 gaaaaatatg atatcgtcca tacacattcc tctaaaactg gtattctagg gcggatagct
     3001 gcgcggctgg caggagttcc ttgtgtagta catactgtac atggctttgc ctttgaaagc
     3061 acaaaaagaa aatcagtaaa gctcgtatat aaatggctgg aaatatttgc agccaaatgc
     3121 actacccgat taatttgtct tcataatgaa gataaagaaa tatgtataaa agaactatat
     3181 gttgatccaa tgaaaatttc tgtaattcca aatggagttg atttggaaaa attcgctcca
     3241 gcaattaata aaggagactt aaaagaaaaa atattagggt taaaaagaaa tagctttgta
     3301 tttaccatgg tgggacggtt gtggccacaa aaaaatccgt tatattttgc tgaagcagca
     3361 aagtatatta tagaaaataa cttaatacct gattcagttt ttgtcatagt tggtgatggg
     3421 gagttgatga atgatttaaa atacaattat caaacagata tgaatttaaa aaaaaggtta
     3481 ttacttttag gctggcgaaa tgatatccca aacatattaa aagcaagtga cgtttttgtt
     3541 cttccttctt tatgggaagg aatgcctctt gccatattag aagctcaatc tacagggtta
     3601 ccatgtatcg tttctaatat caatggtaat aattgtttgg ttaagaatga atttgatgga
     3661 ttcttaattg aacttaatga tattgatagc tttataaatg cgttaattag agtgacagac
     3721 gataaagtct taaacattat gtcaaaaaat tgccgtaata aaattgttaa tgaatttaat
     3781 atagttaaaa gagtggataa aattaaaaat ctatatattg atatggttgt taataaatga
     3841 ttgtgtgatt tttgaatgtt tatatcaata tcgcactgta tattttattt tatgtggatt
     3901 tattatgaat ataaaggcat ggtaattcac atcattattt atatacagac gctgttattg
     3961 gtatagagaa atatcatata acattagttt tcgcacaaaa tgtaaaaaat gaaagattta
     4021 ttactaagta agactatttt tgataaccta tatattatca aatactctgt acataaagga
     4081 gggaaatgga acatgaaaaa taatagtcgt atttctgtta accaattcca aacaacctgg
     4141 gaaagtggag cgtaaattat tagatattta ctactgatta tttgctcctg cttcgtcatt
     4201 tctgacgcag aagtatggga gtatacaaag gatgtttgtg agacgtatgt cgatttcttg
     4261 aagcaatacg atgatttggg acatggtact cttgttcata ataccatagc tgagttctat
     4321 tccgtatcag tcctattaac tggttacgtg atgttattct tcagattata aagacatcat
     4381 tgcagtaggt ggaacttaat agcggagcag aataaagagt ttaaaatgat attcaatatt
     4441 gtatcagttc tgctggttgt ttaaattctg agaagtccgc gatcgtgatt tgaaactgct
     4501 agaatgttga gaataatctg cgctggtgtc tggatgtagt aatgaatgaa gagaagtgct
     4561 caataagata agcaaagcag ctatggacag tgattagccc gcatcaattc ttgcaggggg
     4621 cgtgttttta ttatctttcc ctgattattg ggtgactgtg tgacttatac attctcttat
     4681 tttgagtgtg atggtaaaaa tgtttgactc tatgcatgtt cgggttgctg taaattttgc
     4741 atcaaagata acatttgctt tgatatctat aattacaatc ccagttataa aatcaaaatt
     4801 aggagtaaat gcttatggtg tcctaacatt ctttctatca atccaggtag ttatattact
     4861 gcttgagggg ggaatgagtg ccacaatggc tagaggacta ttgaataaaa aatataagat
     4921 atacaatata agtaaagcag agtaccaaag tgtttatttt atctttttta tcttagtgtc
     4981 attatttgta ttttcaattt ttattgtctt taatcataat atttccacat tgatttacaa
     5041 ccttgatgat aggaaagtct atagttttat tagtgctgat catgttattt tgctggctgg
     5101 tgcaatgatt gccttgcagt tttatatcat atattatgaa gcattatttg tggcttatga
     5161 aaaacaagtc tcttatggaa ttcatataat agtatttaat gtaatcaaaa ccataatatc
     5221 attattcttg ttgattgttt ttaatcttgg gttggatgtt tattttttag ttcatgtatt
     5281 ttgtaccttt atacttatta ttttattgca cattaaggca ataaaaaacg gattattaga
     5341 aataacaaca ttgcgagtaa aaaaaatata cacaaaaata tcatttatga aagaagaatt
     5401 tcatttttct aaaaatatat tatgcgtgtc cattctttct gcaattgcat tccaagctga
     5461 cagactattt gttacaaaat atatgggtat tagtgctgtt ggaatgtatg gcattgctta
     5521 tacattatca acagtaccaa ctttatttac atcatcattg tataatgtta tctatccaag
     5581 acttattatt ttaataagag agcaaagcag cagtgcgatt tcattttttg aattagtatc
     5641 gaaattaata tatattatca taatacccac ttgtgtgatt atttcgctta attcgaccca
     5701 aatcctaaat atttggatag gaaagcacga aggaataata tcattgctgt taagtattct
     5761 aataatagcg acgttatttc aggggttgca ggtgattcct tttgcactac agatggctca
     5821 agaacgacta aaatttacag ttttcatgaa tgctatattt gtgcccagcc taatattggc
     5881 atattatttg gttagtattc ataaaattct gttatacatc tccatagtgt ggtcggtata
     5941 taatataata acttttttct ctttaactat ttatttatat gctaaaaata aaatgtatac
     6001 aagactactt attttatatt ttaatatatg cttgtgtgct gtcgtaacag tagttatatg
     6061 tagcattttt aaaaatcaag aatctcaaat attcttgtct ctagttaata tttctcttca
     6121 gttttttatg tcgatgagtt tgtgcataat actgttgttc tggaaaacta tatatggagc
     6181 attgagatgt gtttgttgtc ggtagtaatt gcgactcata atcgcgcggg atatgctaaa
     6241 aaaagcatag aaagtattct ttcaattgat gttgaagaat ttcaactaat tattcatgat
     6301 acaagcgata ataatgtact tgaggaattt tgcgctgaaa tccaggatgc tagattaaaa
     6361 tatatccatt gcaatgaatt attgtcgatg acagaaaatt ttaatagagc attagaatat
     6421 gttcagggag aatatactat aatgatagga gatgatgata cgattttatc gactgcgata
     6481 aaatattgta gatatgcgaa agaaaaaaaa attgatgtaa tctcgcagaa gatatctgca
     6541 gagtattgtt ggccggattt tagatctaag atctcagggg attttaacgc atcgtctctg
     6601 atagtgaaca aatttacaag taaccaagtt acttatgagg ggcgtaaaca atactatgaa
     6661 gcagttaaaa actgttttca aggtactgca agcctcccca agttatatca tggtattgtt
     6721 aaaacttctt tattgttaaa gataaaagaa aatacaggta cttactttca tggggttagc
     6781 cctgatattt caattgcatt gtcgttagcg tataactgcg aacgatatat tgaagtggat
     6841 tatccaatta cccttccagg atcttcgggg ggaagtaacg caggtagaag tgcaatgcgt
     6901 actcatgttg gtaaattaga cgacgatcca catatgaaaa gatttaaaag ttatgtctgg
     6961 ccaaatttat tgccaagata tttcagtgta gagactgtgt gggctcaggc aggcttagta
     7021 acaatccaaa aaatagataa taataaagga tttaatttta ttaagttcta tgcattatgt
     7081 ttgattaaac atcgacccta taaaaaattt attattgatg agataaaaaa atataagaaa
     7141 gtaaaaggag ggtttaattg tataatttat gcaaaaatta tttatcacat gtcttatttt
     7201 tatattaatg gaatatcaaa tctttctaaa aaaattataa aatatgtctt caaaaaactt
     7261 aagtctcaag agaagcaaaa aaatataaaa gcaaatgata tttttgaagc taggatgata
     7321 attgaaaagg aattatcaaa ggaaacattt gaatgattat attttctact tttctacttt
     7381 tctacttttt tatttttctt tcattgcttc ctgttttaat taaagaggga gctagaggga
     7441 atggtaaata tttttatttc tttgatttat atatttactt agtattagct agtcttatta
     7501 tttatataat ttgtacacga gatatttttt ctgactccga tacagaacgt tatcttagta
     7561 tattagaata ttatagaata aatggctttt atctttttga taagaactat atttttgctg
     7621 tgtatacata tttgtactct ctatatatag atggttacta tatgtatatg ataatcccac
     7681 ttattaccct gctatattca tattgtaagt tagtaaaacc actaaaatat cctgtttata
     7741 gtttttcttt agtattaata gcaacatttc ctgttttttg gcaattgagc gcaagcgcat
     7801 ttaaacaatg cattgcaata tcttttattc ttttagcatt agtgcatatt atacaaacaa
     7861 aatataaaca agcggtgctt ttatctctta tagctagttc cttccatttc acagcattaa
     7921 cgttttttat attttcgttg tttttctata ttagaaagga aagggtcagt ttaaatttat
     7981 tttttattat ttggtttttt agtgtcattt ttagcataag cggattaaat tcgtatattc
     8041 cttcaattat acctttttat gatttagtgg gtgaaagtaa ggtcaatatt tatcttgaat
     8101 caacaaataa tgcaactggt tttcgtgttg atttcttttt gttttcacta gcccctgtgt
     8161 taattatatt tattaacaga acattagtaa taaaagatca ctatattaaa atgattgcat
     8221 taagttattt atatttcaat tctatatata atataatgtc attcatccca ttttcagata
     8281 gagttgccgc atattcatgg tgtctttccc ccattattct attatggagt gctcttaagt
     8341 gtagatcatc aatttatatg ttaagttcta tagggattgc gttattaaat cctttgattt
     8401 ttatgtgcta taattcaatg tttatttttt taaagtgagg atatatgttt aacggtaaaa
     8461 agattttaat cactggtggt actgggtcat ttggtaatgc agtgttacgt cggtttcttg
     8521 agacagatat tcaagaggtt attattttca gtagagatga aaaaaaacaa gatgagatga
     8581 gaacgctata tcgcaatgat aaaatgaaat ttattatagg tgatattaga gattatacat
     8641 ctgttttagc tgcaactagt gacgttgact atatattcca tgctgcggct ttaaaacagg
     8701 tgccttcatg tgaattttat ccatgggaag cagttaaaac gaatataatt ggcacagaaa
     8761 atttattaaa tgctgcaaaa caaaataaag ttaaaaaaac aatttgtttg agcactgata
     8821 aagctgtgta tccaattaac gcaatgggaa tttcgaaggc tatgatggaa aaagtgatga
     8881 cagcaaaggc taggacgttc aacaatgaag attcagttgt ttgcggaact cgttatggca
     8941 atgttatggc atcacgcggc tctgttatcc ctctgttcat tcagcagatt caaacaaatc
     9001 aaccattaac tataacagac cctgaaatga ctagattcat gatgacgtta gatgatgctg
     9061 tagatctcgt attatttgca tttaataacg ggcaaaacgg tgacattttt gttcagaaag
     9121 cgcctgccgc gacaattgct gtgcttgcta aagcattagt tgaattgatg ggggtgccaa
     9181 accatcctat taaagtagtt ggttcacgtc atggggaaaa gatatatgaa accttactta
     9241 gccgtgaaga aatggcaaaa gcgattgatc atggagatta ttatcagatt caaccagata
     9301 atcgtgatct gaattatgac aaatatttaa gcgaagggag taaaaaaatt gagctgtttg
     9361 atgattacaa ttcccataat acatatagac tcaatgttga tgaaatgaaa aaattattgt
     9421 taaaactcaa tatcattcaa aatgtgttaa atagttaagg ataatttata atgcaaaaaa
     9481 tattgatctt aggtgtgagt ggtatgcttg ggcataccct ctttcgtttt ttatcatctc
     9541 agcaggatct tttagtaact ggtacagtac gccatattac gcaagaaata aaattagtat
     9601 catctaagaa tatagtcctg atgagagatg tctttaacaa agaaaaatta gaaaacatta
     9661 taaattcaaa tgatgttatt ataaattgca ttggcgcaat taagcagaaa tatgataata
     9721 aaaatacaga ttatattcta ctaaattcgt actttccaca tcttcttaat gaaatttgta
     9781 tagaatctaa taaaaggcta attcatttct caacagattg tattttcaac ggtgagaaag
     9841 gaaattatga tgatggtagt ttatcaaatg ttatagatat gtatggtaaa tctaaatatt
     9901 taggtgaggt ttatggtgat aaaacactta ctttaaggac atcattaatt gggcatgaat
     9961 taaaatcatc gtatagtttg attgattggt ttttgaactc aaactcaact gtcaatggtt
    10021 atacaaaagc tattttcagt ggattgccaa ccatagagat tgcgcatttt ttatatgagc
    10081 atgttttaca atcccagttg catggtattt ataatttatc agcagcacca atttcaaaat
    10141 tcgatttatt gactttagtc aatgaagtat acaaacttaa taaaaccatc gttcctaaaa
    10201 gtgactttgt gattgatcgt tctcttgact ctcataggtt gcgttcatta actaagtata
    10261 cacctcctga ctggaatact cttgttgtaa agatgtattt agattactta agtttagggg
    10321 cattgcttaa tgaaagataa cttatctttt tctaaactga tttctgaagc tgagattaac
    10381 tccagaaatc gttctcacct taatttgcat gaatcttttt ctgatcctat acagaaaact
    10441 ttgatttgct gttgttcgaa cacgtatatt cctcctcact atcataaatt tgcccatcaa
    10501 agtgaattgt ttattagatt aaaaggatgt tttagtctat taattttttc tgttgatgga
    10561 gtgcttaccc gtcgtataac tatagatgag gaaaatcctc tttatgagat aaaaccaggt
    10621 gttattcata cggtggtagc tttagacaga cataacattt tacttgaaat taagagggga
    10681 ccgtatgtcg aatctgaagc gaaaaatttt cctgattggg taacattaga aaatagcgat
    10741 aatcaacttg atttagagcg attgcgaaca ttgaaggtcg gtgataagtt atcataacaa
    10801 ggctattata aatttatttt aggtgatatc tatgcttcca ttaatatcta ttattacacc
    10861 gacatataat agagcatata ctctagatcg gttatataat tctttagtaa cacaagactc
    10921 tgaaaaattt gaatggataa taatcgacga tggtagtgca gatgaaacac ggaagaaatg
    10981 tttaggttat ataaatgatt ccatttttaa aatacaatat tacaaacaaa ataatagtgg
    11041 taaacccgca gctatcaatt taggagtaac caaagctgtt ggcgattata tttttattgt
    11101 agatagtgat gatgctttaa cattaaatgc aattggtact atttatgact gtattgtaaa
    11161 atacacagtc gcatctgact ttgaattttc cggcttagga tttagaaaat ctttttttga
    11221 tgggaaaatg ctcggcaaac atattgagcg gaatacgaat tcacctatgc tgttacatag
    11281 cacagattgt aataatttat ttaaagtcga tttagcatat tgttttaaac gagagttgat
    11341 gattaaatat cctttcccaa agtttaaaga tgaaaaattt gttccagaac tgtttatatg
    11401 gaataaaatt acagatgttg ccaaaataat attttttcct aatgttgttg tatattattg
    11461 tgagtatctg gctgatggac ttacaaataa ttttaaaaaa caacttcgac ataatccaat
    11521 tggctttgca gtatactata aagatcagtt ccatagagaa tattctatat gggggaaaat
    11581 aaaaatggca ataagatata tccaatgtat tatatataaa ttcatatagt catgcagggt
    11641 tcatccattc atttaattag ttgctatgat ttttatatga aaatattaca tgttgtacca
    11701 cagttaaagc atggtggtgt cgaaactgct gtttttaatt ctattgaaag gataagaaag
    11761 agtggatatg attttaatgt tttgactatt gaaagaggaa actgctttaa caattcatat
    11821 gtaaaagtag aacactgctt aaattcttct gtatacaatc cagtttgttt ttataaattt
    11881 attaggttta ttcataaaga aaaacctgat ttgatagttt attctttatg gaaatctgca
    11941 ttgttaggaa ttgcattgtg tcttttaaaa ccttttctca aaagtaagcc tagaaccgcg
    12001 ttaattatac ataatactag gtttgcacat tttgttgatt acataataac aaaactttca
    12061 cttaaaataa ccgactctat atattttgat tcggaaaaat caaaagctta ctttctgaat
    12121 agagattatg caacaggcga ggttattagt tttatcgata aaaagatggt ggcaaaaaaa
    12181 tttaataata tcaatggtga aattaagttt gtatttattg gtaggttgca ctctcaaaaa
    12241 ggggttgata gggcattaaa atatatacat ctttttaaaa agcaacatga agaaatacga
    12301 tttgatatat atggtccaga cgaaggagaa ttgaataata ttataaataa ggtaaataaa
    12361 ttaggattgc aggataaagt tgtctataaa ggtgttgtcg ataataacaa agttaatgat
    12421 atattaaccc aatatgactt ttatcttcaa ttcagccatt ttgaaggaat ggcaatgtct
    12481 gttgttgatg cgatgcaggt tggggttatt ccaatcgtta ataatgttgg tgagatacca
    12541 aattacgtaa aaaacaatat taacggcatt atcttagatg ataaaaagct tggtgatgaa
    12601 aactatctta atttaaatat atcatgctta ttaaatatca taaagaatga tggtggatat
    12661 tttttatata ataacgcgat aaagacattt caggataata atacttatgt agatgactat
    12721 ataaagaaaa taataaatgc taacaactga tatcatgtgt taaaattgat tatttaaaat
    12781 ggtttatata gctcctacta aataatttgg ctttttcgta atggctgtgt agccgcaatc
    12841 tcgcagtaac cccctgacag gagtaaacaa tgtcaaagca acagatcggc gtcgtcggta
    12901 tggcagtgat gggacgcaac cttgcgctca acatcgaaag ccgtggttat accgtctcta
    12961 ttttcaaccg ttcccgtgaa aagacggaag aagtgattgc cgaaaatcca ggcaaaaaac
    13021 tggttcctta ctatacggtg aaagagttcg ttgaatctct tgaaacgcct cgtcgcatcc
    13081 tgttaatggt gaaagcaggt gcaggcacgg atgctgctat tgattccctg aaaccatatc
    13141 tcgataaagg cgatatcatc attgatggtg gtaacacctt cttcaaggac accattcgtc
    13201 gtaaccgcga gctttctgca gaaggcttta acttcatcgg taccggtgtt tccggtggtg
    13261 aagagggcgc gctgaaaggt ccttccatta tgcctggtgg gcagaaagaa gcctatgagc
    13321 tggttgcacc gatcctgagc aaaatcgccg cagtggctga agacggcgaa ccgtgcgtta
    13381 cctatattgg tgccgatggt gcaggtcatt atgtgaagat ggttcacaac ggtattgaat
    13441 acggtgatat gcagctgatt gccgaagcct attctctgct aaaaggtggc ctgaacctta
    13501 ccaacgaaga actggcacag acctttaccg agtggaataa cggtgaactg agcagctacc
    13561 tgatcgacat cactaaagac atcttcacta aaaaagatga agacggtaac tacctggttg
    13621 atgtgattct ggatgaagcg gctaacaaag gtaccggtaa atggaccagc cagagtgcgc
    13681 tggatctcgg cgaaccgctg tcgctgatta ccgagtctgt gtttgcacgt tatatctcgt
    13741 ctctgaaaga tcagcgtgtt gccgcgtcta aagttctctc tggtccgcaa gcgcagccag
    13801 caggagacaa ggctgagttt atcgagaaag ttcgtcgtgc gctgtatctg ggcaaaatcg
    13861 tttcttatgc tcagggcttc tctcagctgc gtgcggcgtc tgaagagtac aactgggatc
    13921 tgaactacgg cgaaatcgcg aagattttcc gtgctggctg catcatccgt gcgcagttcc
    13981 tgcagaaaat caccgatgct tatgccgaaa atccgcagat cgctaacctg ctgctggctc
    14041 cgtacttcaa gcaaattgcc gatgactacc agcaggcact gcgtgatgtc gttgcttatg
    14101 cagtacagaa cggtattccg gttccgacct tctccgcagc ggttgcctat tacgacagct
    14161 accgtgctgc tgttctgcct gcgaacctga tccaggcaca gcgtgactat tttggtgcgc
    14221 atacttataa gcgtattgat aaagaaggtg tgttccatac cgaatggctg gattaatctg
    14281 atttaaccaa taataaaatt gaggcccggc gtatattgca ccgggctttt ttttgccaaa
    14341 tatcttatag actaaatttc actgcttaat attaacttaa taaatatcag ctattcttat
    14401 aaagaaaatc tgaattgttt ttcgttgcgt tgaccatctg acaacgtagc gttaaaactt
    14461 ttagctctta tcaggatgct aaaaacatca tgattcacag ttaagttaat tctgagagca
    14521 tgaaatgaaa atcaccattt ccggtactgg ctatgtaggc ttatcaaacg ggcttctaat
    14581 cgcacaaaat catgaggttg tggcattaga tattttacca tcacgcgttg ctatgctgaa
    14641 tgatcggata tctcctattg tcgataagga aattcagcag tttttgcaat cagataaaat
    14701 acactttaat gccacattag atataaatga agcctaccgg gatgctgatt atgtcatcat
    14761 cgccactcca accgactatg atcctaaaac taattatttc aatacatcca gtgtagaatc
    14821 agtaattaaa gacgtagttg agataaaccc ttatgcggtt atggtgatca aatcaacggt
    14881 tcccgttggt tttaccgcag cgatgcataa aaaatatcgt actgaaaata ttatattctc
    14941 cccggaattt ctccgtgagg gtaaagccct ttacgataac cttcatccgt cacgtattgt
    15001 catcggtgag cgttcagaac gcgcagaacg ttttgctgcg ttgttacagg aaggcgcgat
    15061 taagcaaaat atcccgaccc tgtttaccga ttccactgaa gcagaagcga ttaaactttt
    15121 cgctaatacc tatctggcga tgcgcgtggc gtactttaac gaactggata gctatgcaga
    15181 aagtttgggt ctgaatacgc gtcagattat cgaaggtgtt tgtctcgacc cgcgtatcgg
    15241 caaccattac aacaatccgt cgtttggtta tggtggttat tgtttgccga aagataccaa
    15301 acagttattg gcgaattatc agtctgtgcc gaataacctg atttcggcaa ttgtcgatgc
    15361 aaaccgcacg cgtaaagact ttattgccga tgccattttg tcacgcaaac cgcaagtggt
    15421 gggtatttat cgtctgatta tgaagagcgg gtcagataac ttccgtgcgt cttccattca
    15481 ggggattatg aaacgtatca aggcgaaagg cgttgaagtg atcatctacg aaccgatgat
    15541 gaaagaagat tcattcttca actctcgcct ggaacgtgat ctcgccacct tcaaacaaca
    15601 agctgacgtc attatctcca accgtatggc agaagagctt aaggatgtgg cagataaagt
    15661 ctacacccgc gatctctttg gcagcgacta a
//
