LOCUS       CR626927                7749 bp    DNA     linear   BCT 06-FEB-2015
DEFINITION  Bacteroides fragilis NCTC 9343, complete genome.
ACCESSION   CR626927
VERSION     CR626927.1
DBLINK      BioProject: PRJNA46
            BioSample: SAMEA1705957
KEYWORDS    complete genome.
SOURCE      Bacteroides fragilis NCTC 9343
  ORGANISM  Bacteroides fragilis NCTC 9343
            Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Cerdeno-Tarraga,A.M., Patrick,S., Crossman,L.C., Blakely,G.,
            Abratt,V., Lennard,N., Poxton,I., Duerden,B., Harris,B., Quail,M.A.,
            Barron,A., Clark,L., Corton,C., Doggett,J., Holden,M.T., Larke,N.,
            Line,A., Lord,A., Norbertczak,H., Ormond,D., Price,C.,
            Rabbinowitsch,E., Woodward,J., Barrell,B. and Parkhill,J.
  TITLE     Extensive DNA inversions in the B. fragilis genome control variable
            gene expression
  JOURNAL   Science 307 (5714), 1463-1465 (2005)
   PUBMED   15746427
REFERENCE   2  (bases 1 to 7749)
  AUTHORS   Cerdeno-Tarraga,A.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2004) Cerdeno-Tarraga A.M., submitted on behalf of
            the Pathogen Sequencing Unit, Sanger Institute, Wellcome Trust
            Genome Campus, Hinxton, Cambridge CB10 1SA E-mail: amct@sanger.ac.uk
COMMENT     Accession number for plasmid pBF9343 in this genome project:
            CR626928.
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Bacteroides fragilis NCTC 9343"
                     /mol_type="genomic DNA"
                     /culture_collection="ATCC:25285"
                     /culture_collection="NCTC:9343"
                     /type_material="type strain of Bacteroides fragilis"
                     /db_xref="taxon:272559"
     misc_feature    <1..>7749
                     /note="PS_2/PS_A lipopolysaccharide biosynthesis locus;
                     label: PS_A"
     gene            <1..117
                     /gene="wzx3"
                     /locus_tag="BF9343_1301"
     CDS             <1..117
                     /gene="wzx3"
                     /locus_tag="BF9343_1301"
                     /old_locus_tag="BF1369"
                     /note="Identical to previously sequenced Bacteroides
                     fragilis putative flippase Wzx SWALL:Q93QW4 (EMBL:AF189282)
                     (482 aa) fasta scores: E(): 3.8e-179, 100% id in 482 aa,
                     and to Streptococcus pneumoniae putative oligosaccharide
                     repeat unit transporter CPS19cJ SWALL:Q9RQG9
                     (EMBL:AF105116) (481 aa) fasta scores: E(): 3.5e-54, 32.7%
                     id in 480 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|O26447|2.A.66.2.10"
                     /protein_id="CAH07082.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_1301"
                     /db_xref="EnsemblGenomes-Tr:CAH07082"
                     /db_xref="GOA:Q5LFL1"
                     /db_xref="InterPro:IPR002797"
                     /db_xref="InterPro:IPR029303"
                     /db_xref="UniProtKB/TrEMBL:Q5LFL1"
                     /translation="MGQSIKNNFLLNLSTTITGLLFPLITFPYASRILMADGIGQVQFF
                     QSIIDYVSLCTALGIPLYAVREIARIRDNKELRSRTTIEILLLHAILTLVGYIVVFILA
                     KTVAKIEIDASLFFLLSTTLFFNTIGVAWFYQAIEDFKYITLRSLFVRILSLVALFIFV
                     KTKQDLFYYAGILVIGTVGNNIFNFFRLRKYIKLSKGEFKRLNLLRHLIPALKIFILNL
                     VISIYVNLDSVMLGFLKNEESVGYYAAATRLTKAILGIVSSLGAVLLPRFSNMITNGQK
                     EEFQLLANKAASFTIALSLPMSVGLIFMAAPIIHIFCGNGFEPSILTLKLVAPIVLFIG
                     LSGIIGMQILYPQGREKYVIISTMVGACINLLINYLLIPQYGQYGAALGTVIAEFMVTV
                     IMILLGRKYLPINILSKQNLHYLIGSIVISILLAFLFVFPLHEVNYLLIGILLSVIVYY
                     AYLLMIKDTLALQLKKLLLSIFKQ"
     misc_feature    1..60
                     /gene="wzx3"
                     /locus_tag="BF9343_1301"
                     /note="14 probable transmembrane helices predicted for
                     BF1369 by TMHMM2.0 at aa 9-31, 46-65, 85-104, 114-136,
                     143-165, 169-191, 211-233, 248-270, 294-316, 331-351,
                     358-378, 383-405, 418-440 and 445-464"
     gene            114..1208
                     /gene="wcfM"
                     /locus_tag="BF9343_1302"
     CDS             114..1208
                     /gene="wcfM"
                     /locus_tag="BF9343_1302"
                     /old_locus_tag="BF1370"
                     /note="Identical to previously sequenced Bacteroides
                     fragilis putative galactopyranose mutase WcfM SWALL:Q93QW3
                     (EMBL:AF189282) (364 aa) fasta scores: E(): 5.9e-144, 100%
                     id in 364 aa, and to Escherichia coli UDP-galactopyranose
                     mutase Glf or B2036 SWALL:GLF_ECOLI (SWALL:P37747) (367 aa)
                     fasta scores: E(): 2.6e-88, 60.22% id in 352 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS biosynthesis related
                     UDP-galactopyranose mutase"
                     /protein_id="CAH07083.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_1302"
                     /db_xref="EnsemblGenomes-Tr:CAH07083"
                     /db_xref="GOA:Q5LFL0"
                     /db_xref="InterPro:IPR004379"
                     /db_xref="InterPro:IPR015899"
                     /db_xref="InterPro:IPR016040"
                     /db_xref="UniProtKB/TrEMBL:Q5LFL0"
                     /translation="MKKKYDYLIVGAGLYGSVFAYKAQRNGKKCLLIDKRPHNGGNIYC
                     ENIEGINVHKYGAHIFHTSNKEVWDFVNAIVEFNRYTNSPIANYKGKLYNLPFNMNTFY
                     ALWGTKTPEEAKMKIEEQRREAGILTPKNLEEQAISLVGKDIYEILIKGYTEKQWGRKA
                     TELPAFIIKRLPVRFTFDNNYFNDKYQGIPVGGYNKLINGLLDGIEVRTNTDFFQNKEY
                     FKGLADKILFTGKIDEYYNYQFGRLEYRTVEFETQVLDCENYQGNAVVNYTEREVPYTR
                     IIEHKHFEFGTQPKTVISKEYSSEWKDGSEPFYPVNDERNNSLYLEYKKLVDREKNVLF
                     GGRLAEYKYYDMNVIVEKVINSDL"
     misc_feature    114..173
                     /gene="wcfM"
                     /locus_tag="BF9343_1302"
                     /note="Signal peptide predicted for BF1370 by SignalP 2.0
                     HMM (Signal peptide probability 0.844) with cleavage site
                     probability 0.589 between residues 20 and 21"
     misc_feature    558..1160
                     /gene="wcfM"
                     /locus_tag="BF9343_1302"
                     /note="Pfam match to entry PF03275 GLF, UDP-galactopyranose
                     mutase, score 374.6, E-value 8.7e-110"
     gene            1205..2080
                     /gene="wcfN"
                     /locus_tag="BF9343_1303"
     CDS             1205..2080
                     /gene="wcfN"
                     /locus_tag="BF9343_1303"
                     /old_locus_tag="BF1371"
                     /note="Identical to previously sequenced Bacteroides
                     fragilis putative glycosyltransferase WcfN SWALL:Q93QW2
                     (EMBL:AF189282) (291 aa) fasta scores: E(): 1e-121, 100% id
                     in 291 aa, and to Methanobacterium thermoautotrophicum
                     rhamnosyl transferase MTH348 SWALL:O26448 (EMBL:AE000820)
                     (313 aa) fasta scores: E(): 5.5e-14, 29.06% id in 320 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="GT2_Glycos_transf_2|GT2"
                     /protein_id="CAH07084.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_1303"
                     /db_xref="EnsemblGenomes-Tr:CAH07084"
                     /db_xref="GOA:Q5LFK9"
                     /db_xref="InterPro:IPR001173"
                     /db_xref="InterPro:IPR029044"
                     /db_xref="UniProtKB/TrEMBL:Q5LFK9"
                     /translation="MKIFAVVVTYNRLALLKKVIDLLKRQTRQLDSIVIVNNGSTDGTK
                     EWLEQEKNITLINQSNSGGAGGFETGVKYAYENEADWIWMMDDDVFPNIDCLEKLLGWT
                     SISQCIQPRRYYSDDVEVNFEQWLDPITYSKFGYWQEKSFNNGKKFCAINVGCFEGMFV
                     SKSIVNKIGFPDKRFFIAEDDTIYGFAASFYTNVILVSDAIMVRARRSSDRSVSPMYTY
                     YACRNFHLVYESLNLLLDKKNRFLYIKYIYQFVHQIYLSIFLYDNKMKHLISVFRGFYD
                     CFRKKNGATY"
     misc_feature    1214..1720
                     /gene="wcfN"
                     /locus_tag="BF9343_1303"
                     /note="Pfam match to entry PF00535 Glycos_transf_2,
                     Glycosyl transferase, score 101.2, E-value 1.7e-27"
     gene            2085..3389
                     /gene="wzy3"
                     /locus_tag="BF9343_1304"
     CDS             2085..3389
                     /gene="wzy3"
                     /locus_tag="BF9343_1304"
                     /old_locus_tag="BF1372"
                     /note="Identical to previously sequenced Bacteroides
                     fragilis putative polymerase wzy SWALL:Q93QW1
                     (EMBL:AF189282) (434 aa) fasta scores: E(): 2.1e-170, 100%
                     id in 434 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS biosynthesis related integral
                     membrane protein"
                     /protein_id="CAH07085.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_1304"
                     /db_xref="EnsemblGenomes-Tr:CAH07085"
                     /db_xref="UniProtKB/TrEMBL:Q5LFK8"
                     /translation="MTSTSFFIIKAISFIVFVYNLASSDWSNLSRVLLFIISFFVFLLV
                     TNVYQRKVKIVTLGYLVYFFLAFLAQNVGPYAIIYNSHIQNLIQRFPNICVPNDHYTLY
                     FLIFIFVSTVIYIYLLCIDKISIEQKVWVYMPTRQEARKIMMAFFIILLPIWILGNRSY
                     ATILVPFTSYFIISIWKYPFTRKIVVFIIGFIASVLILISQLFSRFIFVQYVFPFILIW
                     FLNNGLLFSRKEKISYKVIGFFLLFCSLAILYGIISEMMKLNANFDGNYTFNDMFEVLS
                     EPQLLQNWLSRQTYRIFDIWSHLGGNIIDYIDQKGYLWGITYVKSFAPILGFDYVSLPL
                     ISANMIGADYAQPGLVAEGYANWGIYGAIINMIFVFFVAELLFDYFLRKQSTLNLLLYI
                     GTFSQVLLDGGTINSIIFMSIFCFLTCLFNAKIKF"
     misc_feature    order(2097..2150,2178..2231,2256..2324,2382..2450,
                     2508..2561,2571..2624,2643..2702,2712..2771,2790..2858,
                     3060..3128,3165..3233,3300..3368)
                     /gene="wzy3"
                     /locus_tag="BF9343_1304"
                     /note="12 probable transmembrane helices predicted for
                     BF1372 by TMHMM2.0 at aa 5-22, 32-49, 58-80, 100-122,
                     142-159, 163-180, 187-206, 210-229, 236-258, 326-348,
                     361-383 and 406-428"
     gene            3400..4443
                     /gene="wcfO"
                     /locus_tag="BF9343_1305"
     CDS             3400..4443
                     /gene="wcfO"
                     /locus_tag="BF9343_1305"
                     /old_locus_tag="BF1373"
                     /note="Bacteroides fragilis putative glycosyltransferase
                     WcfO SWALL:Q93QW0 (EMBL:AF189282) (347 aa) fasta scores:
                     E(): 6.7e-142, 100% id in 347 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="unknown"
                     /protein_id="CAH07086.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_1305"
                     /db_xref="EnsemblGenomes-Tr:CAH07086"
                     /db_xref="InterPro:IPR007345"
                     /db_xref="UniProtKB/TrEMBL:Q5LFK7"
                     /translation="MRKILLTYGDIKTINIGDYIQSIAAKQFFDDDNYIFFNRDELKLY
                     KGEPAKVIMNAWMTYKPYNWPPSSQVYPLFVALHINSSAESRFLSHDSIKYLKKYEPIG
                     CRDYHTMNILKGKGVNAYFSGCLTTTLGKTYKYNGKREGIYIVDPLSYMPNGNNFFEIM
                     KAVVQTVFYMKPVLKILRNYKKNNRFTINISKVGIGRLLLITKSYLLLRKLVVPDVLYN
                     AIFITQFNMSNEYSSESERFARADELLTKMASAQYVITSRIHCALPCLGFETPVVYIRN
                     LSESKKSTCRLGGLESLFNVITVKGENVTSNFFDGLFKRDSSFKNKIDFVEYRDRLINI
                     CNKFMMS"
     gene            4440..5576
                     /gene="wcfP"
                     /locus_tag="BF9343_1306"
     CDS             4440..5576
                     /gene="wcfP"
                     /locus_tag="BF9343_1306"
                     /old_locus_tag="BF1374"
                     /note="Similar to Bacteroides fragilis putative
                     glycosyltransferase WcfP SWALL:Q93QV9 (EMBL:AF189282) (378
                     aa) fasta scores: E(): 8.5e-155, 100% id in 378 aa, and to
                     Streptococcus pneumoniae capsular polysaccharide WciL or
                     sp0353 SWALL:Q9AHC8 (EMBL:AF316639) (372 aa) fasta scores:
                     E(): 5.1e-40, 35.86% id in 368 aa, and to Streptococcus
                     thermophilus Eps3J SWALL:Q8GMA0 (EMBL:AY057915) (366 aa)
                     fasta scores: E(): 4.9e-33, 31.81% id in 374 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="GT4"
                     /protein_id="CAH07087.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_1306"
                     /db_xref="EnsemblGenomes-Tr:CAH07087"
                     /db_xref="GOA:Q5LFK6"
                     /db_xref="InterPro:IPR001296"
                     /db_xref="UniProtKB/TrEMBL:Q5LFK6"
                     /translation="MRDGKPIEILHIVGTRPVGGIGALLKNINTSIDLNKFHFTYVFSA
                     DSNIGDFDNYVRKLGSDIVVFPSYHLKYLFLYLKVIFCFYKRNAKKYDIIHVHSANTGV
                     LDLLFAKIYGIRIRILHSHSTKYSSKKIRSIRNYFLQFPTIYLANTYFACGYKAAEFLF
                     GKKRLEKVYIIHNAIFSKKFIYNATVRERVRVELKIENDCLLLGHIGNFTKEKNHGFLI
                     DILEELLNINDNVKLLLVGDGQLRSEIEEKVKLKRLQKYVCFLGRRTDISELLQAMDFL
                     IFPSFFEGLPVTLIEAQASGLRCVVSDRITLETKITNNISYLNLNRDPSTWAEVVWKLF
                     EDKTFIRKDTSKEICESGYDIHLESEYLERIYLNLLHK"
     misc_feature    5004..5510
                     /gene="wcfP"
                     /locus_tag="BF9343_1306"
                     /note="Pfam match to entry PF00534 Glycos_transf_1,
                     Glycosyl transferases group 1, score 94.9, E-value 1.3e-25"
     gene            5573..6379
                     /gene="wcfQ"
                     /locus_tag="BF9343_1307"
     CDS             5573..6379
                     /gene="wcfQ"
                     /locus_tag="BF9343_1307"
                     /old_locus_tag="BF1375"
                     /note="Similar to previously sequenced Bacteroides fragilis
                     putative glycosyltransferase WcfQ SWALL:Q93QV8
                     (EMBL:AF189282) (268 aa) fasta scores: E(): 7.5e-112, 100%
                     id in 268 aa, and to Bacteroides thetaiotaomicron putative
                     glycosyltransferase BT1709 SWALL:AAO76816 (EMBL:AE016932)
                     (273 aa) fasta scores: E(): 6.4e-25, 32.83% id in 268 aa,
                     and to Bacteroides thetaiotaomicron putative
                     glycosyltransferase BT1645 SWALL:AAO76752 (EMBL:AE016932)
                     (269 aa) fasta scores: E(): 1.6e-24, 31.83% id in 267 aa,
                     and to Erwinia amylovora amylovoran biosynthesis glycosyl
                     transferase AmsE SWALL:AMSE_ERWAM (SWALL:Q46635) (266 aa)
                     fasta scores: E(): 2e-23, 33.33% id in 270 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycosyltransferase"
                     /protein_id="CAH07088.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_1307"
                     /db_xref="EnsemblGenomes-Tr:CAH07088"
                     /db_xref="GOA:Q5LFK5"
                     /db_xref="InterPro:IPR001173"
                     /db_xref="InterPro:IPR029044"
                     /db_xref="UniProtKB/TrEMBL:Q5LFK5"
                     /translation="MKLAVISSIYKNDSLSKIKQSLQSLLNQENVTCDIYLYCDGPVSI
                     DIVHFLDSFDQNSLYIFKQEQNRGLAHALNELLNIVLTKEYEYIARMDADDISMPERFV
                     KQIAFMNSHPDIDCLGTWAIEIDDDGKEYFRKKMPITHEECLELFKKRDCMIHPTVMFR
                     RSYFEKAGLYPEDTYFGEDTMMWAKGFKSGCKFANVPEYLFKFRLDSNFFERRRGWKHA
                     KSIYTLRHRVNRMLGFGWKEDCYALLYAMAKLMPKSILDIIYKTVR"
     misc_feature    5582..6085
                     /gene="wcfQ"
                     /locus_tag="BF9343_1307"
                     /note="Pfam match to entry PF00535 Glycos_transf_2,
                     Glycosyl transferase, score 70.3, E-value 3.4e-18"
     gene            6408..7631
                     /gene="wcfR"
                     /locus_tag="BF9343_1308"
     CDS             6408..7631
                     /gene="wcfR"
                     /locus_tag="BF9343_1308"
                     /old_locus_tag="BF1376"
                     /note="Similar to previously sequenced Bacteroides fragilis
                     putative amino sugar synthetase WcfR SWALL:Q93QV7
                     (EMBL:AF189282) (407 aa) fasta scores: E(): 1.5e-157, 100%
                     id in 407 aa, and to Streptococcus suis Cps7G cps7G
                     SWALL:Q9RFX1 (EMBL:AF164515) (404 aa) fasta scores: E():
                     1.6e-67, 44.92% id in 394 aa, and to Streptococcus
                     pneumoniae capsular polysaccharide biosynthesis protein,
                     putative sp1837 SWALL:Q97P07 (EMBL:AE007475) (408 aa) fasta
                     scores: E(): 1.9e-62, 43.48% id in 407 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DegT/DnrJ/EryC1/StrS family amino sugar
                     synthetase"
                     /protein_id="CAH07089.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_1308"
                     /db_xref="EnsemblGenomes-Tr:CAH07089"
                     /db_xref="GOA:Q5LFK4"
                     /db_xref="InterPro:IPR000653"
                     /db_xref="InterPro:IPR015421"
                     /db_xref="InterPro:IPR015422"
                     /db_xref="InterPro:IPR015424"
                     /db_xref="UniProtKB/TrEMBL:Q5LFK4"
                     /translation="MKIPFSPPYIDEAVINEVVDSLRSGWITSGPKVKALEEEIKSFSG
                     AKEVLCVNSWTSGAIMMLRWLGVKEGDEVIVPAYTYSATALAVLHAGAKPVMVDSGTDF
                     NISVEAVRKAITPKTKAIIPVDIAGFPCDYERIMALVQEPEMVKLFRSESPVQEKLGRI
                     LVMNDAAHSLGARYSSRQRTGCETDVAIFSLHAVKNVTTAEGGAICLNLPKPFDNTELY
                     KELRMTSLNCQTKDAFSKSKAGGWRYDIVGFGMKINMADVNAAIGLAQIREYPELLKER
                     KRVFNAYSDAFSACDWAIVPPSVDGEKESSYHIYALRIKDFTEEQRDRMIDEIAKSEVA
                     VNVHFIPMPMLSFFKSMGYDIKDYPQAYQNFKSEISLPIYPQLDSEKLNFIIETVKAAY
                     ATVIAENR"
     misc_feature    6429..7595
                     /gene="wcfR"
                     /locus_tag="BF9343_1308"
                     /note="Pfam match to entry PF01041 DegT_DnrJ_EryC1,
                     DegT/DnrJ/EryC1/StrS aminotransferase family, score 291.7,
                     E-value 7.9e-85"
     gene            7663..>7749
                     /gene="wcfS"
                     /locus_tag="BF9343_1309"
     CDS             7663..>7749
                     /gene="wcfS"
                     /locus_tag="BF9343_1309"
                     /old_locus_tag="BF1377"
                     /note="Similar to previously sequenced Bacteroides fragilis
                     putative undecaprenyl-phosphate galactose
                     phosphotransferase WcfS SWALL:Q93QV6 (EMBL:AF189282) (198
                     aa) fasta scores: E(): 4.8e-76, 99.48% id in 195 aa, and to
                     Bacteroides thetaiotaomicron glycosyltransferase BT0611
                     SWALL:AAO75718 (EMBL:AE016928) (197 aa) fasta scores: E():
                     5.7e-39, 57.21% id in 194 aa, and to Shigella sonnei ORF8S
                     wbgy or ORF8G SWALL:Q9S0U8 (EMBL:AB028134) (196 aa) fasta
                     scores: E(): 1.6e-38, 55.89% id in 195 aa"
                     /codon_start=1
                     /transl_table=11
                     /product="gnl|TC-DB|H8E4X1|9.B.18.1.2"
                     /protein_id="CAH07090.1"
                     /db_xref="EnsemblGenomes-Gn:BF9343_1309"
                     /db_xref="EnsemblGenomes-Tr:CAH07090"
                     /db_xref="GOA:Q5LFK3"
                     /db_xref="InterPro:IPR003362"
                     /db_xref="UniProtKB/TrEMBL:Q5LFK3"
                     /translation="MIRFFDIVFSLLGILLLSPVFLLLYIAICLESKGGGFYKQLRVGR
                     YGGDFYVYKFRSMRVGADKKGLITVGGRDPRITRTGYLIRKYKLDELPQLFNVLKGDMS
                     LVGPRPEVRKYVDLYTDEQKKVLSVRPGITDYASIEYVDENMILGEASDPDRAYIEQIM
                     PDKIRYNMKYICNRSVKEYFKIIFLTFWSIIR"
     misc_feature    7669..>7749
                     /gene="wcfS"
                     /locus_tag="BF9343_1309"
                     /note="Pfam match to entry PF02397 Bact_transf, Bacterial
                     sugar transferase, score 95.2, E-value 1.1e-25"
     misc_feature    7681..7749
                     /gene="wcfS"
                     /locus_tag="BF9343_1309"
                     /note="1 probable transmembrane helix predicted for BF1377
                     by TMHMM2.0 at aa 7-29"
ORIGIN
        1 tatttattga taggtatact gttatctgtt attgtttatt acgcatattt attaatgata
       61 aaagataccc tcgcattaca actaaagaaa ttattacttt caatatttaa acaatgaaaa
      121 aaaaatatga ctatctaatt gtcggagccg gactttatgg ttctgttttt gcatataaag
      181 cgcaacggaa tgggaaaaaa tgtttgctta ttgacaaacg tccacacaat ggaggaaata
      241 tttattgcga aaatattgag ggtataaatg tacacaaata tggtgcacat atttttcata
      301 cttctaataa agaggtttgg gactttgtaa atgccatagt ggaatttaac cgctatacta
      361 attcccctat tgccaattat aaaggcaaat tatataattt accttttaat atgaatactt
      421 tttatgcttt atgggggaca aaaactccag aggaagcaaa aatgaagata gaagagcaaa
      481 ggagagaagc ggggattctt acgccgaaga atctagaaga acaggctatc tctttagtag
      541 gaaaggatat ttatgaaata ttgataaaag gatatactga gaaacagtgg gggcggaaag
      601 caactgaact tcctgctttt ataatcaagc gtttacctgt cagatttact tttgataata
      661 attactttaa tgataaatat caggggattc ctgttggtgg ctataataaa ttgataaatg
      721 gtttattaga tggtattgaa gttagaacaa atacagactt ttttcaaaat aaagaatatt
      781 ttaaaggtct tgcggataaa attctattta ctggcaaaat tgatgaatat tataattatc
      841 aatttgggag attggaatat cgtactgttg aatttgaaac acaagtgctt gattgtgaga
      901 attatcaagg gaatgcagtt gtgaattata ctgaaaggga agtgccttat actcgtatta
      961 tagagcataa acattttgaa tttggaacac agcccaaaac cgtaatttcc aaagaatatt
     1021 cttcggaatg gaaagatggt agtgaaccct tttatcctgt taatgatgag aggaataatt
     1081 ctttgtattt ggagtataag aaattggtcg atagagagaa gaatgttttg tttggaggga
     1141 gattagctga gtataagtat tatgatatga atgtgattgt ggaaaaagtt ataaatagtg
     1201 acttatgaag atatttgctg tagttgtgac ctataatcgt ttagcattac taaaaaaggt
     1261 catagattta cttaaaagac agacacgtca attagatagt atagttatag taaataatgg
     1321 ctctactgat ggaacaaaag aatggttaga acaggagaaa aatataacat taataaatca
     1381 atctaattct ggtggggctg gtggattcga aacgggggta aaatatgcat atgagaatga
     1441 ggcagattgg atttggatga tggatgatga tgtttttcct aatatagatt gtttggaaaa
     1501 actcttaggg tggacgagta tttctcaatg tatccaacca cgtaggtatt attcggatga
     1561 tgtagaagta aattttgaac aatggttgga tccaattact tatagtaaat ttggttattg
     1621 gcaagaaaaa tcatttaata atggtaaaaa gttttgtgct attaatgtag gctgttttga
     1681 agggatgttt gtttcgaaaa gcattgttaa taaaatagga tttcctgata agcgtttttt
     1741 tatagcggaa gatgatacaa tatatgggtt tgcagctagt ttttatacca acgtaatttt
     1801 agtatctgat gctatcatgg ttcgtgctag aagatcatcg gatcgtagcg ttagcccaat
     1861 gtatacttat tatgcatgtc ggaattttca tttagtatat gagtcactta atttattatt
     1921 ggataagaaa aataggtttt tatatattaa atatatttat caatttgttc atcagattta
     1981 tttatctatt tttttatatg ataataagat gaaacactta atatctgttt ttcgtggctt
     2041 ttatgattgc tttcgtaaaa aaaatggagc tacctattaa ttaaatgact agtacttctt
     2101 tctttattat taaggctata tcttttatag tatttgttta taatttagcg agttctgatt
     2161 ggagtaatct ttctcgagta ttgttattta taatttcgtt ttttgtcttt ttattagtaa
     2221 caaatgtata tcaaagaaaa gtaaaaatag taacactagg ttatttggtc tatttctttt
     2281 tggctttttt agctcaaaat gtaggtccat atgcaattat ttacaattct cacatacaga
     2341 atttgataca gaggtttcct aatatttgtg taccaaatga tcattacact ttatactttc
     2401 ttatttttat ttttgtttca actgttattt atatttatct tttatgtatt gataaaattt
     2461 ctattgagca gaaagtatgg gtatacatgc ctacaagaca agaagctcga aaaataatga
     2521 tggcattttt tataatcttg ttgcctatat ggatattagg gaataggtcc tatgctacaa
     2581 ttctagttcc ttttacaagt tattttatca tttctatttg gaaatatcct tttacaagga
     2641 aaatagtagt atttattatt ggttttattg catcggtatt gatccttatt tcgcaattat
     2701 tttctcggtt tatattcgtt caatatgtgt tcccttttat tttaatttgg tttttgaata
     2761 atggattgct attttcaaga aaagaaaaaa taagttataa agttattggc ttttttctat
     2821 tgttttgttc tttagcgatt ttgtatggta tcatttcaga gatgatgaag ttaaatgcaa
     2881 attttgatgg aaactatact ttcaatgata tgtttgaagt attaagtgag ccgcaattat
     2941 tgcaaaattg gttatctagg caaacatatc gtatttttga tatttggtca catttggggg
     3001 ggaatataat agattatatt gaccagaaag gatatttatg gggaattaca tatgtaaaga
     3061 gctttgcacc tatattaggt tttgattatg taagccttcc attaatttca gcaaatatga
     3121 ttggtgcaga ttatgctcaa cctggtttgg tggcagaagg gtacgctaat tggggaatat
     3181 atggggctat aattaatatg atatttgttt ttttcgttgc agagttattg tttgattatt
     3241 ttttacgaaa acaatctacc ttaaatttgt tactttatat tggaactttt tcccaggtat
     3301 tactagatgg ggggaccatt aattctatta ttttcatgtc tatattttgt ttcttaactt
     3361 gtttgtttaa tgccaaaatc aaattttaat attatatata tgaggaagat attattaaca
     3421 tatggagata ttaaaaccat aaatattggt gattatattc agagtatcgc cgctaaacaa
     3481 ttttttgatg atgataatta tatatttttc aatcgtgatg aattaaagtt gtataaaggg
     3541 gaacctgcaa aagtcataat gaatgcttgg atgacttata aaccatataa ttggcctcct
     3601 tcatctcagg tttatccatt atttgttgca ttacatatca attcttctgc agaaagtcgc
     3661 tttttgtcac atgatagtat taagtattta aaaaagtatg aacccatagg gtgccgcgat
     3721 tatcatacaa tgaatatttt aaaaggaaaa ggtgttaatg cttatttttc gggatgtctt
     3781 actactactt tgggtaaaac ttataaatat aatggaaaac gtgaggggat atacattgtt
     3841 gatccacttt catacatgcc taatgggaat aatttttttg aaataatgaa agctgttgta
     3901 caaactgttt tttatatgaa gcctgtgcta aagattctta gaaattataa aaagaataat
     3961 agatttacta ttaatatatc caaagtagga ataggacgtt tattgttaat aaccaagtct
     4021 tatttgcttt tgagaaagct agtagttcct gacgtattat acaatgcaat atttataaca
     4081 caatttaata tgtctaatga atattcttct gaatcagaaa gatttgctcg ggctgatgaa
     4141 ttattaacta aaatggcctc agctcaatat gtgataactt ctagaatcca ctgtgctctt
     4201 ccttgcttag gctttgagac ccctgtggtt tatattagaa atctatcaga aagcaagaag
     4261 agcacttgtc gattaggggg acttgaatct ctatttaatg tgataactgt gaagggggaa
     4321 aatgttactt ccaatttttt tgatggacta tttaaacgag attcttcttt taagaataaa
     4381 attgattttg tagaatatag agatcgatta attaatatat gtaataaatt tatgatgtca
     4441 tgagggatgg aaagcccatt gaaatactgc atattgtagg gactagacct gttggaggaa
     4501 ttggtgcttt attgaaaaat ataaacactt ctattgattt aaataaattc cattttacat
     4561 atgtgttttc tgccgatagt aatattggag attttgataa ttatgtgaga aaattaggta
     4621 gcgatattgt tgtattccct tcatatcatt tgaaatattt atttttatat ttaaaggtga
     4681 ttttctgttt ttataagaga aatgcaaaaa aatatgatat aattcatgta catagtgcta
     4741 atacaggagt attagacctt ttatttgcaa agatatatgg aatacggata aggattttac
     4801 atagtcacag tacaaaatat tctagtaaaa aaataaggtc cattagaaat tattttttgc
     4861 aatttcctac aatatattta gcaaacactt attttgcatg tggatacaaa gctgctgaat
     4921 tcctttttgg aaaaaagaga cttgaaaaag tttatattat tcataatgca attttttcta
     4981 aaaaatttat ttataatgca actgttagag agagagttag agttgagttg aaaatagaga
     5041 atgactgttt actgttgggg catatcggta actttacgaa agaaaagaat catggttttt
     5101 taatagatat attggaagaa ttattgaata ttaatgacaa tgttaagttg ctgttagtag
     5161 gggatggcca acttagaagt gaaatagagg aaaaagtaaa attaaaaaga ttgcagaagt
     5221 atgtgtgctt tttaggtcgg agaactgata tatcagaatt attacaagct atggattttt
     5281 taatttttcc ttcttttttt gaaggtctac cagtgactct gattgaagcg caagcttctg
     5341 gtttaagatg tgtcgtttcg gatcgtatta ctttggaaac aaagataacg aataatataa
     5401 gttatcttaa tctcaatcga gatccttcaa cttgggctga agttgtttgg aagttgtttg
     5461 aagataaaac ttttatcagg aaagatacgt ctaaagaaat atgtgaatca ggatatgata
     5521 tacatttaga gagtgaatat ctcgaaagaa tatatttaaa tcttttacat aaatgaaatt
     5581 ggctgtcatt tcttctatct acaaaaatga ttcgctttct aaaataaagc aatccttaca
     5641 aagtttgttg aatcaagaaa atgttacttg tgatatttat ttatattgtg atggtcctgt
     5701 ttcgattgat attgtgcatt tcttagactc atttgatcaa aactcacttt atatatttaa
     5761 gcaagaacaa aatagaggat tggctcatgc actaaatgaa ttattgaata tcgttcttac
     5821 aaaagaatac gaatatattg ctcgtatgga tgctgatgat atttccatgc cggaacgttt
     5881 tgtgaagcag atcgcattta tgaattcaca tccggatatt gattgccttg gtacttgggc
     5941 aatagaaatt gatgatgacg gaaaagaata ttttcgaaaa aaaatgccga taactcatga
     6001 agaatgtttg gaactgttta aaaagcggga ttgtatgatt catcctactg tgatgtttcg
     6061 acgttcatat tttgagaaag ctggtttata tcccgaagac acctactttg gagaagatac
     6121 tatgatgtgg gctaaaggtt ttaaaagtgg atgtaaattt gccaatgtac ccgaatatct
     6181 gtttaagttt agattggatt caaacttctt tgaaagaagg cgggggtgga aacatgcaaa
     6241 atctatttat actttacgtc atcgtgttaa tagaatgtta gggttcggat ggaaggaaga
     6301 ttgttatgca ttgctatatg ctatggccaa attaatgcct aaatctattc ttgacataat
     6361 ctataaaaca gtacgttgat tttacttttc ttataaaaca taaactcatg aaaatacctt
     6421 tttcaccacc ttatattgac gaggcggtca tcaacgaagt cgttgattcg ttacgttccg
     6481 gttggatcac atccggtccg aaagtgaagg ctttggagga agaaattaaa tctttctcag
     6541 gagcaaaaga agtgctttgt gtcaattcct ggacatcagg agctattatg atgttgcgtt
     6601 ggctgggagt gaaggagggc gatgaagtga ttgttcctgc ctatacttac agtgcaacgg
     6661 cattggctgt gctccatgcc ggagccaaac cggtaatggt ggattccgga actgatttta
     6721 atatttcggt ggaggctgtt cgtaaagcta ttactcctaa aacaaaggcg attatacccg
     6781 tagatattgc tggttttccc tgcgattatg aaagaatcat ggcactggtg caggaaccag
     6841 aaatggtaaa actgttccgt tcggaatctc ctgtgcaaga aaaattggga cgcatcttgg
     6901 tgatgaatga tgctgcccac tctttgggag cacgttacag tagccgtcag cgtaccggtt
     6961 gtgaaactga tgtggctatc ttttcacttc atgccgtgaa aaatgtgact actgccgaag
     7021 gtggggccat ttgcctgaat ctgcctaagc cgttcgataa tacggagttg tataaagagc
     7081 tacggatgac aagcttgaac tgccagacca aggatgcttt ttcgaaatct aaagccggag
     7141 gatggcgtta tgatattgtc ggtttcggga tgaaaatcaa tatggccgat gtaaatgccg
     7201 ccataggact ggcacagata cgagaatatc cggaattgct gaaagaacga aagcgggtgt
     7261 ttaatgctta tagcgatgct ttttcggctt gtgactgggc catcgttccg ccttctgtgg
     7321 atggggaaaa ggaaagctct tatcacattt atgctttgcg tattaaggac tttaccgaag
     7381 aacaacgcga ccggatgatt gatgaaatcg ctaaaagtga agtggctgtc aatgtgcact
     7441 ttataccgat gccgatgttg tcgttcttca aatcgatggg atatgatata aaggattatc
     7501 cacaggctta tcagaacttt aagagcgaga tatcattgcc gatatatccc caattggata
     7561 gtgaaaaatt gaatttcatt attgaaaccg taaaggcggc ctatgcgacc gtaattgcag
     7621 aaaaccgata gtatatttac aaacactaaa tagataagtt gcttgatccg tttttttgat
     7681 atcgtttttt cccttttggg aattcttctg ctttctcccg tttttcttct tctttatata
     7741 gccatttgt
//
