LOCUS       JABKKG010000006        15363 bp    DNA     linear   BCT 25-MAY-2020
DEFINITION  Prevotella sp. PINT PINT_6, whole genome shotgun sequence.
ACCESSION   JABKKG010000006 REGION: 163785..179147
VERSION     JABKKG010000006.1
DBLINK      BioProject: PRJNA630669
            BioSample: SAMN14906829
KEYWORDS    WGS.
SOURCE      Prevotella sp. PINT
  ORGANISM  Prevotella sp. PINT
            Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales;
            Prevotellaceae; Prevotella; unclassified Prevotella.
REFERENCE   1  (bases 1 to 15363)
  AUTHORS   Galvez,E.J.C., Iljazovic,A. and Strowig,T.
  TITLE     Distinct polysaccharide utilization as determinants for
            interspecies competition between intestinal Prevotella spp.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15363)
  AUTHORS   Galvez,E.J.C., Iljazovic,A. and Strowig,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-MAY-2020) Microbial Immune Regulation (MIKI),
            Helmholtz Centre for Infection Research - HZI, Inhoffenstrasse 7,
            Braunschweig 38124, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: JUL-2017
            Assembly Method        :: SPAdes v. 3.10.0
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 394.23x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/20/2020 15:13:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 4.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,852
            CDSs (total)                      :: 2,788
            Genes (coding)                    :: 2,750
            CDSs (with protein)               :: 2,750
            Genes (RNA)                       :: 64
            rRNAs                             :: 5, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 3, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 3 (5S)
            tRNAs                             :: 53
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 17 of 38
            Pseudo Genes (incomplete)         :: 15 of 38
            Pseudo Genes (internal stop)      :: 10 of 38
            Pseudo Genes (multiple problems)  :: 4 of 38
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15363
                     /organism="Prevotella sp. PINT"
                     /mol_type="genomic DNA"
                     /submitter_seqid="PINT_6"
                     /strain="PINT"
                     /isolation_source="feces"
                     /host="Mus musculus"
                     /culture_collection="DSM:103738"
                     /db_xref="taxon:2736291"
                     /country="Germany: Braunschweig"
                     /collection_date="Jul-2017"
                     /collected_by="Iljazovic/Lesker"
     gene            1..2229
                     /locus_tag="HPS57_09935"
     CDS             1..2229
                     /locus_tag="HPS57_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="NPD82286.1"
                     /translation="MKRTSLLALTFAATVASSAATEKRDTTASVTQEKKEERNVMLNA
                     SDANKPREIQIGLPSEDINVYENGLPAVYSSAVHKLSAHWRSDNSLGEVSLMTPSESA
                     IATGNIAYSVNSNSRWGEKDFHAVLSYKANHFGWQNFDMNVSGEITDNLLFTASVYQN
                     FDPGSFKLKFTDYADRTQLYHLGLTHLLPNNRGRISLMYKHSRNENPGNFSSAAPFIY
                     KGDGSVEELPGFKLGTSSYVPHNGSFHYMDIMTGEMKTWNLGDDDRNMANELTLVADY
                     RFNKEWKGSLSFKWMDAPRAKYVDFGGSSISEVCADDNYMLDDGSIYTGLKEGRRTWL
                     HDGKVKNVLLTTELQKTFRTNTLKLGLNQWHYSLDYHSASMQWDATVEEYPRMLNSTY
                     GYNELSPEYTKGYENKLAFYATDQWQATRRLNIFAGARLEYYRMNADRIPMTRFNGFH
                     IGATSPVDGSTVAVENIVKNKLNYAATLRATYTLNHGFGLTADATVATRFPRINEYAG
                     TGPTEEQYKRVTIPLVRGGMTYRNSWLDLTSMVSYIGKTNNIDQQNLTRPGSTESKTV
                     LLIYGIQTIGWTTSAEITPFKGAHLHALLTLQKPTYKDYNASVTFSDGTGMAVNANGN
                     IVKEIPQTIIELDPSYNITDDLRLWLSFRYFGKTYANLQESLFFNGRWESFGGINWKV
                     NKNLSLNATVINFLNQKGASGTINGSELISKEDAHLFAGHYMSGNYLRPFTLEFGMKL
                     EF"
     gene            2248..4482
                     /locus_tag="HPS57_09940"
     CDS             2248..4482
                     /locus_tag="HPS57_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="NPD82287.1"
                     /translation="MKPVLLSLLLLSSATNIDAQRIDISTSKTDLILKVGDNKRLYQT
                     YLGEKLLNKSDGDILPWHVKGGSDGSVSERGWEVYPGSGAEDYYEPALAMTHYDGNLT
                     TILQYSDHTQQQTPFGAITEIRLKDKLYPVEVTLHYESYYKENIIKTWATISNNGKSA
                     VALWRYASAMLYFNRSEYYLTEFSSDWAKEAQMRTQPLCFGKKVIDTKLGSRAAMHTH
                     PFFEIGLDTPASENRGEVLMGTLAWTGNFQFTFEVDNVGNLRILPSINPYASTYEVAA
                     KESFATPEFIFTLSQHGTSEGSRDLQRWARLYQLKDGMGDRMTLLNNWENTYFDFDQN
                     LLAQLMNEAKELGVDMFLLDDGWFGNKHPRKNDRAGLGDWQPTKSKLPGGIHALTESA
                     KTAGVKFGLWIEPEMVNPKSELFEKHPDWAYTLPDRKTYYYRNQLVLDLNNPEVQEHV
                     YKVVEDILRNNPDVAYFKWDCNSPITNIFSHYLKEKQQQLYIGHVRGLYKVLQRIKKN
                     FPDTPMMLCSGGGARCDYEALKYFTEFWCSDNTDPVERLFIQWGFSQFFPAKAMCAHV
                     TSWNKQTSVKFRTDVASMGKLGFDIGLKDLSPTDLLYVQNAIKNWNRLKPAILDGVQY
                     RLVSPYESNHTATMYTTEDATKAVLFAFDIHPRFAEKLHKVKLQGLIPTANYRVKEIN
                     VMPMIDTTKSAKQRSSAAPNPSYKSTWASDNKVFSGDFLMKVGLDVFTAQETQSHVIE
                     LTKE"
     gene            complement(4599..6431)
                     /locus_tag="HPS57_09945"
     CDS             complement(4599..6431)
                     /locus_tag="HPS57_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="NPD82288.1"
                     /translation="MKRYIKQITGVFALSSLALLSSCSLDEKNPGGFTFDTVTMTEDG
                     YQAILNQIYFGAERVLYGTDGYMQLTEADTDLWTTKANDGNSNTQYFWFFGGASANTT
                     YTNTIWNAIYDGIGSCNTAIAYAANPPISEAKRNNSIAQARFMRAVYYFNAVEQFGGV
                     VKVTDLGVPNYAPERTDPLTIYKEVIIPDLEFATQYLDKGTDATCTTPTKKSALGFLA
                     KACLQTYEYGSTEFLQKGMDAAKALITDCENGGSQYGAYMYSNFEDVFKQDNSWENKE
                     ALWKHRWYSGSDGHGSSNGAQKCNRNDEMFLAAINHFAARQDNPQARQTWEGGQTGSF
                     MPTRHLLNLFVQEDGSLDPRFHKSFVTEWNCNRDYTWTDGDAKTYGKDASVVGKTLKF
                     QENILDKTQNSKAIGDLAIKFVMPQDAGYASEVANAANASYLLVDYKKVYSDADRNVI
                     MTSGNAENHWRYFYPSLSKHNSSNIYVVNESKNRFANLNATFIMRMAEVYLIAAELDI
                     YLNGGSGAMAYINKVRTRAGANALSGTASVRTVLDERGRELCGEYSRFYDLKRTGMFK
                     DANYLQETHPDLAKFFTPEYALRPISTTYLATITNGAGWQNPGY"
     gene            complement(6444..9527)
                     /locus_tag="HPS57_09950"
     CDS             complement(6444..9527)
                     /locus_tag="HPS57_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="NPD82289.1"
                     /translation="MQKKKTFVNKAMKAMLVAGFAATMSPLSALAGGSVNTFVQARQN
                     AIKGTVVDEAGEPMIGVTIKVKNSQAAAITDLDGNFSVNASDGATIEISYVGYITQTV
                     KAHNGMKVQLAPDSQVMDEVVVIGYGTVKKRDVTGAVASIKNEDIVIAPTSNAMEALQ
                     GKVAGMDIVKGSGQIGDEPEILLRGTRSIYGSNEPLFIIDGIPGSYSEVNPSDIETID
                     VLKDASSTAIYGSAGANGVVIITTKRGQTGKVRVNFDAYYGWSGAPNYENSMTGDEWV
                     NYHQEAYKYEHGNYAADMATLFGNQDYLDAYNAGKWIDWVDLVSGNKATTQKYSLSVT
                     GGTDKTKIYASAIYSKDKGLLSNEQRDKYALRLNVDQEINKWAKVGFTSNLTYSNHDR
                     GVKNTFTRALTAFPLGDAYDAEGNIVSEYINGQYSPLGDFIPNQYANETHTTYVNATG
                     FLELTPFKGMSIRSQISGSTSHARQGLYWGALCNANRPTYAGTPHAEKVFNDSWSYTW
                     ENVFAYNTTIAKDHSIGGTFITSWSESQSEPTTAGGSGQTMDQWLFHRLNAATSQYIK
                     SEYTQTQKMSYAVRFNYSYKGRYLFTASNRWDGVSWLSEGNKWESFPAAAVAWRISDE
                     AFMGGMSSWLDNLKVRIGWGITGNSGGIGAYGTQSQPYAYSSSGVTVDGKIVPFYQYT
                     GTYASPDLTWEKSYNWNIGLDFSVLNSRIDGSVEWFSTKTRGLLFARQMPSTTGITGW
                     GSPLKVWQNLAKTSNHGVEFTINSRNIRTKDFSWNTSLTGTWQKERIEELPDGDLIAE
                     NLFVGSPIKAVYGYKYAGIWGTDTDKATLDAYGVEPGFIKVETNEKFDAKGVSDNGVH
                     KYGEDDRRILGHRNPNWILGLNNTFTYKNLDLTIYAMGRFGQTIESSMLGYYTADNSL
                     TKNQPSGVDYWTEDNQGAFYPRPGTGGKQATVYSSLRVVDGSFIKIKNITLGYTLPKS
                     WTRKALIENCRVYFTTYNPFIFSMDSRLNDTDPETGGADSFPTYKQYVIGINLTF"
     regulatory      10370..10552
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            10553..12919
                     /locus_tag="HPS57_09955"
     CDS             10553..12919
                     /locus_tag="HPS57_09955"
                     /inference="COORDINATES: protein motif:HMM:NF019335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="NPD82290.1"
                     /translation="MKKSVLSLGIIMSTHLAYAGGVSEKDAGKSGSVSPTELSDTTIT
                     LQEVSIRGNFCNERETPLNLTTITPQDIRLHFTSPNYVEMLNGVPGVYATASTGSYGD
                     ASLNMRGFKQDNISIMLNGIPIQGLTSGSMYWSNWMGLAEATYSVQVQKGMGGSMLAD
                     CAMGGMVNIITKTGNGLPNGSFALSVTDEGLTKGVLNYSTGTLRHGWNIDAMVSYTRG
                     HGFVECSDVSTFSYMLSVSKLLGAHNTLIFTALGSPETHDQRNTELSAAEVEKYGRGY
                     SKNWGWLNGEKYSIGRNHYFKPYFTLQHILDGERFSMKNSVYVAIANGGGRSTYGQKG
                     SIPIISHQTTDGHIDFDAVQAENRASADGSQNIMLDYLSGHTQAGAIASAEYLVTKEM
                     KVLGGVQYQYYDTWSKMKVLDLLGGNHWYDTTSKQNVGVGDYTGARYGRTTHHASGYI
                     QGQYATERLTANIGASLFSGTYSRHNDITGERSRWAHGFGGGVRGGVLYHILKGDAGG
                     NRDLSVYLNGGYNSRLPYAGVYLASSDLSITNDIVNEKNLLGELGVRASWQGGGAELS
                     GYIASWRDKTLTVNITKRADEAAEKYNITGLNALHRGIELAVHQRLLPWLRAKAFFST
                     ASWRWKSDGKGVTYDSFSGETLKEYKIYCNNLHVGDAPQTQAGLTLDGRIPLSAALGS
                     KAGALYFNAGWQLNARMYADFEPSSRTDEDAPDAYRLPSYSLVDATVGWEGSVSKGLR
                     LNLFACCRNLFDAHYIERGIDGTTHDLESFRGYWGAPRQFSFGMRLAF"
     gene            complement(13033..15363)
                     /locus_tag="HPS57_09960"
     CDS             complement(13033..15363)
                     /locus_tag="HPS57_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="NPD82291.1"
                     /translation="MRRFLQHIAIMALTLIVSLSAKAAVITQSGWFETAYAKWEPVNG
                     ASDYQVFIKPVGGTYTQLDKELVRQYPGYYRADAVGLSAGSYVMKIVPVISGTADESK
                     AMETAAMNVMPHDRNGFAHYNRSEGVGAYNNDGTLKSNARVIYVTGNNAKTITLGVMQ
                     DKEVSFTGLQAIIGAFEKGKETRPLAIRIIGTIKGEDMDKFGSSAEGLQIKGKSGAYN
                     ANITLEGIGNDAAIHGFGILCRSISSVEFRNFAIMLCMDDCLSLDTDNHNIWIHNMDF
                     FYGSTGGDSDQAKGDGTVDIKGKSSHVTVSYNHFFDSGKCSLGGMKSETTDCWMTYHH
                     NWFDHSDSRHPRIRTAFYHVYNNYFDGNSKYGVGVTSGGSSFVESNYFRNCKYPMLIS
                     KQGTDAEGSGTFSGEPGGVNKAYNNFIKGERKVQYYDGSQTDGKWDAVLAKSRDESVT
                     ATALAGGSSYNNAADTEARKAVPASAIETPEAARTTTQAYAGRCEQGDFKWTFNNSIE
                     DENYGVISELKNAVVNYQSTLIGFADGTAIKNGGAQPAGDGLAVDGGNGKGIDPDKNE
                     EYVPSWAGGGGTITSGDYVIGTDKEYFWTIADNATETRKLLDNGTITLNEGSSFQTTS
                     NVAGSDGTVFSDKVGSLQLVKSTGTATFYWKDGIQSIAFYIARTGSMKGDILTSDDGT
                     TFTKHSDYTGNKGTKDIAVTPAEPCKYVRITNTATGSLHIQGIKIATVSGSTGVDGIE
                     TVVSDIERMYDITGRRIASPVKGQLYIKGGKKHIAQ"
ORIGIN      
        1 atgaaaagaa catctctact tgctttgacc tttgcggcaa ccgtcgcaag cagtgcagcc
       61 acggaaaagc gtgacacaac agcatccgtc acgcaggaaa agaaggagga acgcaacgtc
      121 atgcttaacg cctccgacgc caacaaaccg cgtgaaatcc aaatcggact gccaagcgag
      181 gacatcaatg tctatgaaaa cggactccct gccgtctatt cttccgctgt gcataagctc
      241 tccgcccatt ggcgcagcga caattccctt ggcgaagtgt cgctcatgac tccttcggaa
      301 tcggcaatcg ccacgggaaa catagcatac tccgtcaaca gcaacagccg atggggagag
      361 aaagacttcc atgccgtact ctcatacaag gctaaccact tcggttggca gaactttgac
      421 atgaacgttt caggggaaat caccgacaat ctgctcttca cagcgtccgt ctatcagaac
      481 ttcgacccgg gcagtttcaa actgaaattc acggattatg ccgaccgcac acagttatat
      541 catctcggtt tgacgcacct tctgccaaac aaccgcggac gcatatccct catgtataag
      601 cattcacgta acgaaaaccc gggcaatttt tccagtgctg caccgtttat atataaaggc
      661 gacggcagcg ttgaggaact tccgggcttc aaactgggaa caagttctta tgtgccacac
      721 aacggttctt tccactacat ggacattatg accggcgaaa tgaagacttg gaaccttggg
      781 gacgatgacc ggaacatggc aaatgagctt actctggtcg ccgattaccg cttcaacaaa
      841 gaatggaagg gcagcctctc gttcaaatgg atggacgctc cacgcgccaa atatgtggat
      901 ttcggtggaa gctccatatc agaagtctgt gccgacgaca actatatgct tgacgacggt
      961 tctatatata ccggactgaa ggaaggcaga agaacttggc ttcatgacgg caaggtgaaa
     1021 aatgtccttc tcacaacgga gctgcagaag actttccgca ccaacacact gaaactcgga
     1081 cttaaccaat ggcactattc actggactat cattcagcct ccatgcagtg ggacgccaca
     1141 gtcgaggaat atccccgtat gcttaactcc acttatggct ataacgaact gagcccggaa
     1201 tacacaaaag gctacgaaaa caaacttgcc ttctatgcca cggaccaatg gcaggcaacg
     1261 cgacgcctta acatcttcgc cggagcacgc cttgagtact accgcatgaa cgctgaccgc
     1321 atcccgatga cccgcttcaa cggcttccat attggagcga cttcgcctgt cgacggctca
     1381 acagtggctg tggaaaacat tgtgaaaaac aagctcaact atgccgccac gctccgtgcc
     1441 acctacactt tgaaccatgg tttcggactt accgcggacg caaccgtagc gacacgattc
     1501 ccacgcatta acgaatatgc cggcaccgga ccgacagaag agcagtacaa acgcgtaacc
     1561 atccctctcg tccgtggcgg aatgacatac cgcaattcat ggcttgacct cacttccatg
     1621 gtcagctaca tcggaaagac aaacaacatc gaccagcaaa acctcacacg ccccggaagc
     1681 accgaaagca agacagtcct gctcatctac ggcatacaaa ccatagggtg gacaacatcg
     1741 gctgagataa cgcccttcaa aggggcacac ctccacgctt tgctgacact ccaaaagcct
     1801 acatacaagg attacaacgc atcagtgaca ttttctgacg gcaccggcat ggctgtgaat
     1861 gccaacggga acattgtaaa agagattcca cagacaataa tagaacttga cccaagctat
     1921 aacatcaccg atgacctgcg tctttggctc agtttccgct atttcggcaa gacctacgca
     1981 aaccttcagg aatcactttt cttcaacgga cgctgggaat ccttcggcgg aataaactgg
     2041 aaagtgaaca aaaacctcag cctcaacgcc actgtcatca acttcctaaa tcaaaaaggg
     2101 gcaagcggca caatcaacgg aagcgaactt atctcgaaag aggatgctca tctgtttgcg
     2161 ggacattaca tgagcggcaa ctatcttcgt ccgttcacac ttgaatttgg catgaaactt
     2221 gaattttaac ctaacaatat acaaacaatg aaacctgtac tactatcatt attgctctta
     2281 tcttcagcaa cgaacatcga cgcacagcgc atcgacatct caacatcaaa aaccgacctg
     2341 atactgaaag tgggagacaa caaacgcctc taccaaacct atctcgggga aaaactactg
     2401 aacaagtccg acggtgacat cctgccttgg cacgttaagg gcggcagcga tggaagtgtc
     2461 tcggaacgag gatgggaagt ttatcccggt tccggtgcgg aggactacta cgaaccggca
     2521 cttgccatga cccactatga cggaaatctt acgacaatcc tgcaatattc cgaccataca
     2581 cagcagcaga cgcccttcgg agcaataacg gagatacgcc tgaaggataa gctctaccct
     2641 gtggaagtga ctctccatta tgagtcctac tacaaagaga acatcataaa gacatgggca
     2701 acaatcagca acaacggtaa aagtgcggtg gcattgtggc gctatgcctc ggcaatgctc
     2761 tacttcaacc gcagcgaata ctatctcact gagttctcaa gcgactgggc aaaggaggcg
     2821 cagatgcgca cccaaccgct ctgtttcgga aagaaagtca tcgacacaaa gttgggaagc
     2881 cgtgccgcca tgcacacaca cccgttcttc gagataggat tggacacgcc tgcaagcgag
     2941 aaccgtggcg aggttctcat gggaacactt gcatggactg ggaatttcca attcactttt
     3001 gaggtggaca atgtggggaa tctgcgcatc ctgccgtcca taaaccctta tgcctcaacc
     3061 tacgaggtgg cggcaaaaga gagttttgcc acacctgagt tcatcttcac actatcacag
     3121 cacggcacca gcgagggaag ccgcgacttg cagcgttggg caaggctcta ccaactcaaa
     3181 gacggcatgg gcgaccgcat gactttgctc aacaactggg agaacactta cttcgatttc
     3241 gaccagaatc ttcttgcgca acttatgaac gaagcaaagg aactcggagt ggacatgttc
     3301 ctcctcgacg acgggtggtt tggcaacaag catccccgta agaacgaccg ggcaggtctt
     3361 ggcgactggc aaccaacgaa atcaaaactg cccggtggca tccacgccct tacagagtca
     3421 gcaaaaacgg caggagtgaa attcggacta tggatagaac ccgagatggt aaaccctaaa
     3481 agtgaacttt ttgagaaaca tcctgactgg gcttacaccc tccctgaccg caagacatac
     3541 tactatcgca accaacttgt ccttgacctc aacaaccctg aggttcagga gcatgtctac
     3601 aaagtggtgg aagacatact taggaacaac ccggacgtgg catatttcaa atgggactgt
     3661 aattctccga taacaaacat attctcacac tacctcaagg agaagcagca gcaactctac
     3721 atcggtcatg tgcgcggact ctacaaagtg ctgcagcgca tcaagaagaa tttccccgac
     3781 accccaatga tgctctgctc gggtggcgga gcacgctgcg actacgaggc actgaaatac
     3841 ttcaccgagt tctggtgctc tgacaacaca gaccctgtgg aacgactctt catacaatgg
     3901 ggcttctccc aattcttccc tgccaaggct atgtgcgccc atgtgacaag ctggaacaag
     3961 cagacttccg tgaaattccg caccgatgta gcgtcaatgg gcaagttggg ctttgacatc
     4021 ggactcaaag acctctctcc cacagacctc ctctatgtcc aaaacgccat aaagaactgg
     4081 aaccgtctga aaccggcaat cctcgacggt gtgcagtacc gtctcgtttc gccttacgaa
     4141 agcaaccaca ctgccacaat gtacaccacg gaagatgcca caaaggctgt cctcttcgcc
     4201 ttcgacatac accctcgctt tgccgagaag ctccacaaag tgaagctgca aggtctgatt
     4261 ccaacggcaa actatcgcgt taaagaaata aatgtcatgc ctatgataga cacaacaaag
     4321 tccgcaaagc agagaagttc tgccgctcct aatccgtcat acaagtcaac atgggcttcc
     4381 gacaataagg tcttcagcgg cgattttctc atgaaggtcg gtcttgatgt gttcactgcg
     4441 caagagacac agagccatgt catcgaactg acaaaggagt aatgtcatga aataagagac
     4501 cttctaacac aacaaatccg tgatacatgc cctcttttga gagtgtatca cggattacat
     4561 gttaatcaga cgactgaaca agtcgtcagt tcattttatc aatagccggg gttctgccat
     4621 ccggcgccat tggtgattgt cgccaaatat gtcgtggaga tagggcgcag agcatactca
     4681 ggagtgaaga acttagccag gtcagggtgt gtctcctgca gatagttggc atccttgaac
     4741 ataccggtgc gcttcaggtc atagaaacgg ctgtactcgc cacacaactc cctgccacgc
     4801 tcgtcaagca cagtgcgtac agaggcagtg cctgagaggg cgtttgcccc tgctcttgtg
     4861 cgcaccttgt taatatacgc catcgcacca ctgccaccgt tgagataaat gtcaagctcg
     4921 gcagcgataa ggtaaacctc agccatgcgc atgatgaacg tagcattcag gtttgcgaaa
     4981 cggttcttgc tctcgttaac aacatagatg ttgctggagt tgtgcttgct caatgaagga
     5041 tagaagtaac gccagtggtt ctctgcattg ccggaagtca tgattacgtt acggtcggcg
     5101 tcactgtaca ccttcttata gtccacaagc agataagaag cattggcagc attggcaacc
     5161 tccgatgcgt aaccggcatc ctgcggcatg acaaacttaa tggcaaggtc accgatagcc
     5221 ttggaattct gtgtcttgtc aaggatgttc tcctggaatt tcagggtctt tcctacgaca
     5281 gaagcgtcct tgccataagt ctttgcgtcg ccgtccgtcc atgtgtagtc acggttgcag
     5341 ttccactctg tgacaaagga tttgtggaaa cgcgggtcaa gtgagccgtc ttcctgcacg
     5401 aaaaggttga gcagatgccg ggtaggcatg aagctgcctg tctgtccgcc ttcccatgtc
     5461 tgtcgcgcct gtggattgtc ctgacgcgca gcgaaatggt tgatggcagc gaggaacatc
     5521 tcatcattgc ggttacactt ctgcgcaccg ttggatgagc catggccgtc gcttccggaa
     5581 taccagcgat gcttccacaa agcctccttg ttctcccagc tgttgtcctg cttgaagaca
     5641 tcctcaaagt tggaatacat gtatgctcca tactgcgaac cgccattctc acagtcggtt
     5701 ataagagcct ttgctgcgtc catacccttc tgcaggaact ccgtagaacc atactcgtaa
     5761 gtctgaaggc aagcctttgc gaggaatccc aatgccgatt tctttgtggg agtcgtgcaa
     5821 gttgcgtcgg ttcccttgtc aagatattgc gtagcgaact caaggtcagg gataatcacc
     5881 tccttatata ttgtcaatgg gtcagtgcgc tcaggcgcat agttaggcac gccaaggtca
     5941 gtgaccttca ccactccgcc aaactgctcc acagcattga aatagtaaac agcgcgcatg
     6001 aagcgagcct gtgctatgct gttgttgcgc tttgcctcgg aaataggcgg attggcagca
     6061 taagcgatgg cagtgttgca gcttccgata ccgtcataaa tagcattcca gatagtgttt
     6121 gtgtatgtcg tgttagcact cgcaccgcca aagaaccaga aatactgtgt gttgctgttg
     6181 ccgtcattgg cctttgttgt ccaaaggtct gtgtcagcct ctgtcaactg catgtagccg
     6241 tcagtgccgt agaggacacg ctccgcgccg aaataaatct ggttaaggat agcctgatag
     6301 ccgtcctctg tcatggtcac agtgtcgaag gtgaagcctc ccgggttctt ctcgtccaag
     6361 gagcaagagc tgagaagtgc caatgatgat aatgcgaaga ctcctgttat ctgctttatg
     6421 tatcttttca taagtctgtt tcgttagaag gttaggttga tgccgatgac atactgcttg
     6481 taggtcggga agctgtcagc tccgccggtc tcagggtcag tgtcgttaag gcgtgagtcc
     6541 attgagaaga tgaacggatt gtaggttgtg aagtagacgc ggcagttctc tatcaatgcc
     6601 ttgcgtgtcc agctctttgg caatgtgtag ccgagagtga tgttctttat cttgatgaaa
     6661 gagccgtcaa cgacacggag tgaagagtag acagtggcct gcttgcctcc tgtacccggg
     6721 cgtggataga acgctccctg attgtcctct gtccaatagt cgacgcctga cggctggttc
     6781 tttgtcagac tgttgtctgc cgtatagtag ccgagcatgc tgctctctat ggtctggccg
     6841 aaacgtccca tggcatagat tgtcaggtca aggttcttgt aagtaaaggt gttgttcaga
     6901 ccgagaatcc agttagggtt tctgtgaccg agaatgcggc gatcgtcctc gccatacttg
     6961 tgcacaccat tgtcagaaac acctttagca tcaaacttct cgtttgtctc aacctttatg
     7021 aagcccggct ccacgccata agcgtcaagc gtagccttgt cagtgtcagt gccccagatt
     7081 cctgcatatt tgtatccata gacagccttt atcggtgagc cgacgaacag gttctcagcg
     7141 atgaggtcgc cgtcagggag ttcctctatc ctttctttct gccatgttcc ggtgagagat
     7201 gtgttccaag agaagtcctt tgtccttatg ttacggctgt tgatagtaaa ctcaacgccg
     7261 tggttgcttg tcttggcaag gttctgccat accttcaatg gtgagcccca gccggtgatg
     7321 cctgtagtgc tcggcatctg tcgtgcgaag agcaggccac gagtctttgt gctgaaccac
     7381 tcgacagagc cgtcgatgcg gctgttaagg acagagaaat ccagaccgat gttccagttg
     7441 taggacttct cccatgtgag gtcaggactt gcgtaagtgc ctgtgtactg gtagaaaggc
     7501 acgattttgc cgtccactgt cacgccggaa ctgctgtaag cgtaaggctg tgactgtgtg
     7561 ccgtaagcgc cgatgccgcc ggagttgcct gtgatacccc aaccgatgcg caccttcagg
     7621 ttgtcaagcc aactgctcat gcctcccatg aacgcctcgt cagagatgcg ccatgccacg
     7681 gcagctgccg ggaaactctc ccatttgttg ccttcggaaa gccatgagac accgtcccaa
     7741 cggttggagg ctgtgaagag atagcgtcct ttgtaagaat agttgaagcg tacggcataa
     7801 gacatcttct gagtctgtgt gtactcagac ttgatgtact gtgatgttgc agcgttcagg
     7861 cggtggaaaa gccattggtc cattgtctgt ccggaaccgc cggcagttgt cggctcgctc
     7921 tgtgactctg accatgatgt gatgaacgtg ccaccgatgc tatggtcctt tgctatggtc
     7981 gtgttgtatg cgaacacatt ctcccatgta tagctccagc tgtcgttgaa caccttctcg
     8041 gcatgaggcg ttcccgcgta tgtagggcgg ttggcattac agagcgcccc ccaatagagt
     8101 ccctggcgag cgtgggatgt ggagcctgaa atctggcttc gtatgctcat ccccttgaac
     8161 ggagtaagct caaggaaacc ggtggcattc acgtaagtag tgtgggtctc gttggcatac
     8221 tggttaggga tgaagtcgcc aagcggagaa tattgtccgt tgatatactc tgaaacgatg
     8281 tttccctcag catcgtaagc atcgccgaga gggaacgcag tgagcgcgcg ggtgaatgtg
     8341 ttcttcacgc cacggtcatg gttagagtaa gtaaggttgg atgtgaaacc aacttttgcc
     8401 cacttgttta tctcctgatc gacattcagg cgcagagcat acttgtcgcg ctgctcgttg
     8461 gaaagcaggc ccttatcctt ggaatagata gcggaagcat agattttcgt cttgtctgtg
     8521 ccgcctgtta ctgacaagct gtatttctgc gtggtagcct tgttgccaga aacaaggtcc
     8581 acccaatcaa tccacttgcc tgcattgtaa gcgtcaagat agtcctgatt gccgaacagc
     8641 gtagccatgt cggcagcata gttgccgtgc tcatatttgt aagcctcctg atgatagttc
     8701 acccactcat cgccggtcat gctgttctca tagtttgggg caccgctcca accatagtaa
     8761 gcatcaaaat tgacacgcac cttgcctgtc tgtccgcgct ttgtggtgat gataaccaca
     8821 ccgttggcac cggcagaacc gtagatagcc gttgaagagg catccttcag cacgtcaata
     8881 gtctcgatgt ccgaagggtt cacctcggaa tagcttcccg gaatgccgtc gatgatgaac
     8941 agcggctcgt tgcttccgta tatggagcgt gtgccacgga gcaggatttc cggctcatcg
     9001 ccaatctgtc cggaaccttt cacgatgtcc ataccggcaa cctttccctg caaagcctcc
     9061 atggcattgc ttgtaggagc gatgacgatg tcctcgttct ttatggatgc cacggcaccg
     9121 gtgacatcgc gcttcttcac cgtgccgtag ccgatgacca ccacctcgtc catcacctgc
     9181 gagtcgggag caagctgcac cttcattccg ttgtgcgcct tcacggtctg cgtgatgtat
     9241 cccacgtagg agatttcgat tgtcgcaccg tcagaagcgt tcactgagaa gttgccgtca
     9301 aggtctgtga tggcggcagc ctgtgagttc ttcaccttga ttgtcactcc aatcatcggt
     9361 tcgcctgcct cgtcgacaac agtgcccttg attgcattct gccgcgcctg cacaaaagtg
     9421 ttcacactgc caccggcaag tgccgacaga ggtgacatcg ttgctgcaaa accggctacc
     9481 aacatagctt tcatagcttt gttgacaaaa gttttcttct tttgcataaa tgttaagtta
     9541 gttctaataa aaatgtgact tgtatgcaca tgtaactaaa gattatgtgg cgcaaattta
     9601 gcaaaataaa tatataatgg tagtatctac actatccaat taacacattt tacccccccc
     9661 gataattttt aacagtatta aacacaaaga aagaaaaaat atataaaaag tgctgtgcta
     9721 tattgaatgc ttgtatcatc tacattttac atgttccgga cacaaccaca ggcattatcc
     9781 ttcactctct actgcattgc cctatattgc cgccggagtc cgccacaagg tttgcggcgg
     9841 tgatgtgctg cctttgcaat tgtgagtcac atcattttgc aaacaccggc aaaaccacac
     9901 atacggcgaa aggtcgtgag tgacaaaatg aaagcctacc ttttgcatga tgaaaggtaa
     9961 cgattggctt ggcaaaaggt aacgattcac aatgcaaagt gttaccttta gttatgttaa
    10021 ccgtatttta atattcatta agtttttagc ttacacaaaa cacaaataca catatcattt
    10081 tgaaagcaat aacacaagtt ttgcttacac atttccaaaa gaggattaca ggactaacaa
    10141 tgttgaggcg gacaaccttg gactgccccg aaaatagcat catataataa tgtacggttg
    10201 cacctccgca tgcccgaata tcgtcttttt tattgccctt accacgattt ttttgcaata
    10261 gataatggaa aatacatttt tttttataac tttgcagccg gaaaaacgaa gacaaggcaa
    10321 caggaggatg ctcatgactg ccaagactga gtttgccaca acataaaaga aaaatcttca
    10381 gggcagggtg taattcccga tcggcggtaa agtccgcgag cctgaaacgc ttcacgcaac
    10441 gccataggac ataacggaaa atggcaatgc ggagggcgtg gaaggcagaa ccgttgaagc
    10501 gaaagtcgcc attacggtac cgacagtaaa gtctggatga aagaagaaaa gaatgaaaaa
    10561 atctgtttta tcgctgggca ttatcatgtc cacacacctc gcctacgccg gtggcgtaag
    10621 cgagaaggat gccggcaagt ccggctctgt cagtccgacg gaactttccg acacgacaat
    10681 cacacttcag gaggtttcca tccgtggcaa tttctgcaac gagagggaaa cgccgcttaa
    10741 ccttacaaca atcactccgc aggacatacg cctgcacttt acgtctccca actatgtgga
    10801 gatgctcaac ggtgtgccgg gagtctatgc cacagcctcc acgggcagct atggcgatgc
    10861 gtcgcttaac atgcgcggat tcaagcagga caacatctcc ataatgctta acggcatccc
    10921 tatccaggga cttacctcgg gcagcatgta ctggagcaac tggatgggct tggcggaggc
    10981 gacctattcc gtgcaggtgc agaagggcat gggtggctcg atgcttgccg attgtgccat
    11041 gggcggtatg gtgaacatca tcacaaagac cggtaacggt ctgcctaacg gctcgtttgc
    11101 cctctccgtc actgacgagg ggcttacgaa aggtgtgctg aactactcta ccgggacatt
    11161 gcgtcatggg tggaacatcg atgcgatggt gtcgtacact cgcggacatg gcttcgtgga
    11221 gtgctcggat gtaagcactt tctcctatat gctgagtgtc agcaaactgc tcggtgctca
    11281 caacacactc atcttcacgg cactcggttc gccggagacg cacgaccagc gcaacacgga
    11341 gctttccgct gcggaggtgg aaaagtatgg gcgcggctac tcaaagaact ggggttggct
    11401 caacggggag aagtattcca taggcagaaa ccattatttc aagccgtatt tcacccttca
    11461 gcatatcctt gacggtgagc gtttctccat gaaaaactcc gtgtacgttg ccatagccaa
    11521 cggcggcgga cgctccacct acggacagaa aggcagcatt ccgataataa gccaccagac
    11581 aactgacggg catatcgact ttgatgccgt acaggcggag aaccgtgcgt cggcggatgg
    11641 ttcgcagaac atcatgctgg actatctttc gggacacact caggcgggtg cgatagcatc
    11701 ggcggagtat cttgtcacta aggaaatgaa ggtgcttggt ggcgtccagt accaatatta
    11761 cgacacatgg tcgaagatga aggtgcttga ccttctcggc ggaaaccatt ggtatgacac
    11821 cacttccaag caaaatgtcg gtgtgggtga ctacaccgga gcgcgttacg gcaggaccac
    11881 gcaccatgct tcggggtaca ttcaggggca gtatgccacg gagcgcctga ccgcaaacat
    11941 cggtgcgtcg ctgttcagcg gtacatacag ccgtcataat gacatcaccg gcgagcgcag
    12001 ccgttgggct cacggcttcg gaggaggagt gaggggcggt gtgctctacc atattctgaa
    12061 aggggatgcc ggcggaaacc gtgatttgtc ggtgtatctt aacggcggtt acaactcacg
    12121 cctgccgtat gccggtgtgt atcttgcatc aagcgacctg tctatcacca acgacattgt
    12181 caatgaaaag aacctccttg gcgagcttgg agtgcgcgcc tcatggcagg gcggtggtgc
    12241 ggaactgtcg ggatacattg cttcatggcg tgacaagacg ctgacagtga acatcaccaa
    12301 gcgtgccgac gaggctgccg agaagtacaa cataacaggc ttgaacgccc tgcaccgtgg
    12361 cattgaactt gccgtgcatc agcgactgct gccttggctg cgtgcgaaag cgttcttctc
    12421 cacagcctca tggcgctgga agagtgacgg caagggcgtg acttacgact ctttcagtgg
    12481 agaaacattg aaggaataca agatttactg caacaatctg catgtcggcg atgcaccgca
    12541 gacgcaggca ggtcttaccc ttgacggcag gattcctctg tcagcagcct tgggaagcaa
    12601 ggcgggcgca ttgtatttca atgccggttg gcagctgaat gcgcggatgt atgctgattt
    12661 tgagccatca tcacgcacag acgaggatgc tcctgacgcc taccgtctgc cgtcctacag
    12721 tcttgtggac gccaccgtcg gctgggaagg aagcgtaagc aaaggtctgc gcctgaacct
    12781 tttcgcttgt tgcaggaatc tgtttgatgc gcattacatt gagcgcggca tcgacggaac
    12841 gacacacgac cttgagtcgt tccgcggata ttggggcgca ccgcgacagt tcagcttcgg
    12901 catgcgcctt gcattttaga gttgttgttt ggagaaatat acaaaaatcg gtaaacggta
    12961 aaaccaaaaa tgccgtgggc ttatcaagtc cacggcattg tcattaatgt gttttctgaa
    13021 atatattaga tattattgtg cgatatgttt ctttccgccc tttatgtaca gctgtccctt
    13081 tacaggagaa gcaatcctgc gtcctgtgat gtcgtacatg cgctcgatgt cggagacaac
    13141 agtctcgata ccgtcaacgc cggtagaacc agacacagtg gcaatcttta ttccttgaat
    13201 gtgcaggctg cctgtagcgg tatttgtgat gcggacatac ttgcaaggct ctgccggagt
    13261 aacggcaatg tcttttgtgc ctttatttcc ggtgtaatcg gaatgctttg tgaaagtcgt
    13321 tccgtcgtca ctcgtgagaa tatcaccctt catcgaacct gtgcgtgcaa tatagaaagc
    13381 tatgctttga atgccgtcct tccaatagaa ggtcgctgtg cctgtactct ttacaagctg
    13441 gagtgagccg actttgtcag agaagacggt tccgtcgctt cctgcaacat tgcttgtggt
    13501 ttggaatgac gaaccttcat taagggtgat tgtgccgttg tcaaggagtt tccgggtctc
    13561 agtggcattg tcggcaatgg tccagaaata ctctttgtct gttcctatga cgtaatcacc
    13621 ggaagtaatt gtaccgccgc cacctgccca tgaagggaca tactcctcgt ttttgtcagg
    13681 gtcgatgccc ttgccgttac cgccgtcgac agcgagtccg tcgcctgccg gctgtgcacc
    13741 tccgttcttg attgctgtgc cgtcagcgaa accgatgaga gtagactgat agtttactac
    13801 agcattcttg agttcggaga tgactccgta gttctcgtcc tctatggagt tgttgaaagt
    13861 ccacttgaaa tcaccttgct cgcagcgtcc ggcgtatgcc tgtgtcgtag tgcgtgcagc
    13921 ttccggtgtc tctatcgcac ttgccggcac tgccttgcgt gcttccgtgt cggcagcatt
    13981 gttgtaggat gagcctccgg caagtgctgt cgcggtgaca gactcgtcac gtgatttcgc
    14041 aagtaccgca tcccatttgc cgtcggtctg tgagccgtca taatactgaa ccttgcgttc
    14101 gccctttatg aagttgttgt aagccttgtt cacgcctccc ggttcgccgg agaatgtgcc
    14161 gctgccttct gcgtcagtac cctgcttgga gattagcata ggatacttgc agttgcggaa
    14221 atagttgctt tccacgaaag aagagccgcc gcttgtcacg cctacaccat actttgaatt
    14281 gccgtcaaag tagttgttgt agacatgata gaacgcagtg cggatgcgtg gatggcgtga
    14341 atcggagtgg tcaaaccagt tatgatgata tgtcatccag cagtctgttg tctcgctctt
    14401 catgcctccg agtgagcatt tgccggaatc aaagaaatgg ttgtatgaga ctgtaacgtg
    14461 gcttgacttt cctttgatgt cgacagtgcc gtcgcccttt gcctggtcgg aatcgccacc
    14521 ggtgctgcca tagaagaagt ccatgttgtg aatccagatg ttatggttgt cagtgtcaag
    14581 ggacaaacag tcatccatgc agagcattat ggcgaaattg cggaattcca cagaggaaat
    14641 gctgcgacag aggatgccga agccatggat ggcagcgtca ttgcctatgc cttcgagagt
    14701 gatgtttgca ttataggcac cgcttttgcc ttttatctgc agtccttccg cgctcgaacc
    14761 gaacttgtcc atgtcctcgc ccttgattgt gcctatgatg cggattgcga gagggcgtgt
    14821 ctccttaccc ttctcaaatg cgccaataat agcctgaagt cctgtgaaag acacctcttt
    14881 gtcctgcatc acaccaagag tgattgtctt agcgttgttt cctgtgacat aaatcacgcg
    14941 tgcattgctc ttcagtgtgc cgtcattgtt gtatgcgccg acaccttcgc tgcggttgta
    15001 gtgtgcgaaa ccgttgcggt cgtgcggcat gacattcatt gctgcggtct ccattgcctt
    15061 gctttcgtcg gctgtgccac tgatgacagg cacaatcttc atgacgtatg agccggcact
    15121 gagtcctacg gcatcggcac ggtagtagcc gggatattgg cgcacaagct ccttgtcgag
    15181 ctgtgtgtat gtgcctccta ccggttttat gaacacttgg tagtcggaag ccccgttcac
    15241 cggctcccac tttgcatagg ctgtctcaaa ccatccgctt tgcgtgatga cagcagcctt
    15301 tgcagacaat gacacgatga gtgtcaaagc cataatggca atgtgctgca aaaatcttct
    15361 cat
//

