LOCUS       NZ_KB290676             5912 bp    DNA     linear   CON 19-MAR-2021
DEFINITION  Anaerostipes hadrus DSM 3319 Scfld194, whole genome shotgun
            sequence.
ACCESSION   NZ_KB290676 REGION: 26972..32883
VERSION     NZ_KB290676.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02436796
            Assembly: GCF_000332875.2
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Anaerostipes hadrus DSM 3319
  ORGANISM  Anaerostipes hadrus DSM 3319
            Bacteria; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae;
            Anaerostipes.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Weinstock,G., Sodergren,E., Lobos,E.A., Fulton,L., Fulton,R.,
            Courtney,L., Fronick,C., O'Laughlin,M., Godfrey,J., Wilson,R.M.,
            Miner,T., Farmer,C., Delehaunty,K., Cordes,M., Minx,P.,
            Tomlinson,C., Chen,J., Wollam,A., Pepin,K.H., Bhonagiri,V.,
            Zhang,X., Suruliraj,S., Warren,W., Mitreva,M., Mardis,E.R. and
            Wilson,R.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAY-2012) Genome Sequencing Center, Washington
            University School of Medicine, 4444 Forest Park, St. Louis, MO
            63108, USA
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            KB290676.1.
            Anaerostipes hadrus DSM 3319 is a member of the Firmicutes division
            of the domain Bacteria and has been isolated from the GI tract.
            
            This is a reference genome for the Human Microbiome Project. This
            project is co-owned with the Human Microbiome Project DACC.
            
            The sequenced strain was obtained directly from ATCC (ATCC 29173).
            Source DNA was prepared by Michelle Daigneault and Emma
            Allen-Vercoe (Department of Molecular and Cellular Biology,
            University of Guelph, Ontario, Canada), and was funded by the NHGRI
            (through an HMP Technology Development Grant - 1R21HG005811 - 01).
            
            Coding sequences were predicted using GeneMark v3.3 and Glimmer3
            v3.02. Intergenic regions not spanned by GeneMark and Glimmer3 were
            blasted against NCBI's non-redundant (NR)  database and predictions
            generated based on protein alignments. tRNA genes were determined
            using tRNAscan-SE 1.23 and non-coding RNA genes by RNAmmer-1.2 and
            Rfam v8.1. The final gene set is processed through several programs
            such as Kegg (Release 56), psortB (Version 3.0.3) and Interproscan
            (Version 4.7) to determine possible function. Gene product names
            are determined by BER (Version 2.5). Gene names are generated at
            the contig level and may not necessarily reflect any known order or
            orientation between contigs.
            
            The National Human Genome Research Institute (NHGRI), National
            Institutes of Health (NIH) is funding the sequence characterization
            of the Anaerostipes hadrus DSM 3319 genome.
            Eubacterium hadrum DSM 3319 is a member of the Firmicutes division
            of the domain Bacteria and has been isolated from the GI tract.
            
            This is a reference genome for the Human Microbiome Project. This
            project is co-owned with the Human Microbiome Project DACC.
            
            The sequenced strain was obtained directly from ATCC (ATCC 29173).
            Source DNA was prepared by Michelle Daigneault and Emma
            Allen-Vercoe (Department of Molecular and Cellular Biology,
            University of Guelph, Ontario, Canada), and was funded by the NHGRI
            (through an HMP Technology Development Grant - 1R21HG005811 - 01).
            
            Coding sequences were predicted using GeneMark v3.3 and Glimmer3
            v3.02. Intergenic regions not spanned by GeneMark and Glimmer3 were
            blasted against NCBI's non-redundant (NR)  database and predictions
            generated based on protein alignments. tRNA genes were determined
            using tRNAscan-SE 1.23 and non-coding RNA genes by RNAmmer-1.2 and
            Rfam v8.1. The final gene set is processed through several programs
            such as Kegg (Release 56), psortB (Version 3.0.3) and Interproscan
            (Version 4.7) to determine possible function. Gene product names
            are determined by BER (Version 2.5). Gene names are generated at
            the contig level and may not necessarily reflect any known order or
            orientation between contigs.
            
            The National Human Genome Research Institute (NHGRI), National
            Institutes of Health (NIH) is funding the sequence characterization
            of the Eubacterium hadrum DSM 3319 genome.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Finishing Goal           :: High-Quality Draft
            Current Finishing Status :: High-Quality Draft
            Assembly Method          :: Velvet v. 1.1.04
            Genome Coverage          :: 103x
            Sequencing Technology    :: Illumina
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/19/2021 01:29:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 5.1
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 2,711
            CDSs (total)                      :: 2,653
            Genes (coding)                    :: 2,580
            CDSs (with protein)               :: 2,580
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 5 of 73
            Pseudo Genes (frameshifted)       :: 26 of 73
            Pseudo Genes (incomplete)         :: 33 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 10 of 73
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Anaerostipes hadrus DSM 3319"
                     /mol_type="genomic DNA"
                     /submitter_seqid="Scfld194"
                     /strain="DSM 3319"
                     /isolation_source="stool, GI tract"
                     /host="Homo sapiens"
                     /culture_collection="ATCC:29173"
                     /type_material="type strain of Anaerostipes hadrus"
                     /db_xref="HMP:0369"
                     /db_xref="taxon:649757"
     gene            complement(1..1386)
                     /locus_tag="HMPREF0369_RS08180"
                     /old_locus_tag="HMPREF0369_01715"
     CDS             complement(1..1386)
                     /locus_tag="HMPREF0369_RS08180"
                     /old_locus_tag="HMPREF0369_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="WP_009203922.1"
                     /translation="MKFLDVEKYTPIQKSHEAYLKELMEYCKDTPRHPSYHIHPPCGL
                     VNDPNGLAYFGGKYHVFYQWFPFGPEHGMKHWAHVISEDLIKWERSDQMLIPDQEYEK
                     NGCYSGNSIEADGKLYLYYTANYKTEQGKIPKQAMAVMNSDGTILKSPNNPIIDEQPE
                     GLIGEIRDPFVFEKEGAYWMLLGGGSTDGQARLILYKSTDLENWVYQGNIELTGIDLE
                     LGYMYECPSYIEIDGKDVLFLSLMGRTPMGERFHNEFSSVYFIGELNLEDKTFHVESF
                     DEIDKGFDFYAPQAFYGKDRQPMMFAWLGCGVQELPYAKEDMWIHSLTMPRFLTIKEG
                     KLCQEVPENIKNEYAHLDIDSKRIKPEEDTWYINLTDKQISEIQIGDQEDHLSIKIDW
                     AAGHIVADRSTLKHQFSTEYGIQREVSMSEELKNIEIYYDNTFIEIYLNDGKDTMTLR
                     AFPENVEINLI"
     gene            complement(1388..3295)
                     /locus_tag="HMPREF0369_RS08185"
                     /old_locus_tag="HMPREF0369_01716"
     CDS             complement(1388..3295)
                     /locus_tag="HMPREF0369_RS08185"
                     /old_locus_tag="HMPREF0369_01716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBCA"
                     /protein_id="WP_008394277.1"
                     /translation="MDYKKISQDVVDHVGGISNIKGATHCVTRLRLILNDETKYDRKA
                     LENIEGVKGVLFNSGQLMIIFGTGTVNNVYDAFIELTGVKEMSQSDAKAEGMSKMGKL
                     QQGFKVFSDIFIPIIPAFVAAAILTGVKALLTANGLFGLEGSLADQSKLVADIAEFLA
                     IMATTFNYLPILVMYSAVKRFGGNPILGILVGIVMVHPDLVNRNTFVLDPSAASYWHF
                     GPLAIAKVAFQGGVFPAILTAWFMSKVEKIAQKYVPAVVSFVFVPTITIFFANVALFT
                     VFGPVGNMIGNGLAAVIDVLYNSFGAFGAFVFAALLQPLVVTGTHQAIQGIEANLIAT
                     TGFNYIQGIWSVSIIAQGGGAIGMYLLAKKHSKDRDIAMSSFIPTLVGISEPAIFAAN
                     LKYSVIPFVCSCLGAGCGGAFMKLAGVRAIGQGLTGILGLLIVVPNKLVFYVIGNLIA
                     FIVPIVLIVIYNKTKGVPHGEEEEEAESALTIDETAKVPVKAVVDGKVIAIEDVKDGI
                     FSEKVLGDGVGIIPTSETVLAPAEGEICTVMEASKHAIGVRTTNNSVFLIHVGIETVS
                     MNGEGFEYLVKKGQHVKEGQPILKFDKAKIEAKGLNPVVVFVKTDEGNQTPVAFKTGI
                     NVKAGKDVIGE"
     gene            complement(3325..4716)
                     /locus_tag="HMPREF0369_RS08190"
                     /old_locus_tag="HMPREF0369_01717"
     CDS             complement(3325..4716)
                     /locus_tag="HMPREF0369_RS08190"
                     /old_locus_tag="HMPREF0369_01717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="WP_009203923.1"
                     /translation="MYQLYYQPEGIWVGDIMPYGKEGQFYVYHQRDTRNPGPFGEPFG
                     WALATTKDFVDYKDYGESLKRGTDEEADQFIYAGSVFETEAGFHAFYTGYNREFLKAG
                     KTSQTLLHATSKDGITWEKSKEALEIPPQEGYDKRNWRDPFVLWDEEREEYLLILGAR
                     KGEDKRKQTGRLVHFTSKDLKKWEFKGDFWAPGIYTMFEMPEIFKMGDWWYLVFSEYS
                     EGNKIHYRRSKNLYGPWEAPFDDAFDGRAYYAGRTAFDGERRVLFGWVPTRIDNDDKN
                     AYLWGGTFVPHEVFQKEDGTLGVKPVDQMMEAFDGWKDLFNPCMKTIDTKEETLLCED
                     TGSIAAFKTTVKFEEGTKEFSIRFYKDEETEVSYEYRFFVEENKVVFNKCPNYPWYQC
                     FNIGLERPIKLEADKEYEICLIIDQDISTLYINGTALNARLCDHPGNGLALTVTDGTL
                     EAKNTKIATKINK"
     gene            complement(4935..5912)
                     /locus_tag="HMPREF0369_RS08195"
                     /old_locus_tag="HMPREF0369_01718"
     CDS             complement(4935..5912)
                     /locus_tag="HMPREF0369_RS08195"
                     /old_locus_tag="HMPREF0369_01718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="WP_008394275.1"
                     /translation="MKPGIKDVAKVAGVSPTTVSRVLNNRGYISEETRKKVYDAMEEI
                     NYYPNEIARALLNNRTYFVGVIVPTVTSPFHGEVVEQIEYYLSQKNYKMLLCNSKNQM
                     DTEKAYIDMLRRNQVDGMIVGTHNAVVETYSKLKMPVVGIDRYLGEHIPVVSCDNYAA
                     GQMATRHLIDKGCQHILCIRGNSKLKMPGNNRSQAYIQEMEKVGLPQMILEVPFIMEN
                     VEKQKLIYDMLNAHPEIDGIFAGDDSLAVIALHVARQKGINIPKDLKIIGVDGTKQIL
                     GFVPELTTIQQPVKQIAKVAVDKLIDLIEGKTAESCMDLPVKLLEGQTT"
ORIGIN      
        1 ctaaattaaa ttaatttcca cattttctgg aaatgcacga agggtcattg tatcttttcc
       61 atcatttaga taaatttcaa taaatgtatt atcataatat atctcaatgt ttttgagttc
      121 ttctgacatg gaaacttctc tttgaattcc gtattctgta ctaaactgat gcttaagtgt
      181 acttcggtct gcaactatat gtcctgctgc ccagtcaatc ttaatactta gatgatcttc
      241 ttgatctccg atctgaattt ctgagatctg tttatctgtc agattgatat accatgtatc
      301 ttcttctggt ttaattcttt tactgtctat atcaagatgt gcatattcat tcttaatatt
      361 ttctggcact tcctggcata actttccttc tttgatcgtt aagaaccttg gcattgtcag
      421 actatggatc cacatatctt cttttgcata tgggagttcc tgtaccccac acccaagcca
      481 cgcaaacatc attggttgtc tgtcttttcc atagaatgcc tgtggtgcat aaaaatcaaa
      541 tcctttgtcg atctcatcaa atgattccac atgaaatgtt ttatcttcaa gatttaactc
      601 tccgataaaa tatacactgg aaaactcatt gtgaaaacgt tctcccattg gtgtacgtcc
      661 cattaatgat aagaataaaa catcttttcc atctatttca atgtaagatg gacattcata
      721 catataacca agctccaggt caattcctgt cagttcaata tttccctgat atacccagtt
      781 ttcaagatct gtactcttat acaaaatcaa tcttgcctgc ccatcggtgc ttccaccacc
      841 taaaagcatc cagtatgctc cttctttttc aaagacaaat gggtctctga tctccccgat
      901 cagtccttct ggctgttcat cgatgatcgg attgtttgga gatttaagaa ttgtaccatc
      961 actgttcata actgccattg cctgttttgg gatctttccc tgttctgtct tatagtttgc
     1021 tgtataataa agataaagct taccatctgc ttctatggaa tttcctgaat aacatccatt
     1081 tttctcatat tcctgatctg gaatgagcat ctgatcagac cgttcccatt ttatgagatc
     1141 ttccgagatc acatgtgccc agtgtttcat tccgtgttct ggtccaaatg gaaaccactg
     1201 ataaaacaca tggtattttc ctccaaaata cgccagtcca tttggatcgt tcactagtcc
     1261 acatggggga tgaatatgat aggatggatg ccgtggagta tctttacaat actccataag
     1321 ctcctttaaa tatgcttcat ggcttttttg gatcggtgta tatttttcta catctaaaaa
     1381 tttcatatta ttccccgatc acgtcttttc ctgctttgac attgattcct gtcttaaatg
     1441 ccacaggtgt ctggttacct tcatccgttt tgacaaatac gacgactgga tttaatcctt
     1501 ttgcttcaat ctttgctttg tcaaatttga gaattggctg accttccttc acgtgttgtc
     1561 ctttctttac aagatattca aatccttctc cattcatgga tactgtttcg attcctacat
     1621 ggatcagaaa tacactattg tttgttgttc ggacaccgat ggcatgtttt gaagcttcca
     1681 tcactgtaca gatttcacct tctgccggcg ctaagactgt ttcacttgtt gggatgatcc
     1741 ccaccccgtc tcctaatact ttttcagaga agattccatc ttttacatct tcaatcgcaa
     1801 taacttttcc gtcaacaact gctttgactg gtactttcgc tgtctcatca attgttaatg
     1861 cagattcagc ttcttcctct tcttcaccat gtggaacacc ttttgtttta ttataaataa
     1921 cgatcagtac gattggtacg ataaatgcga tcaggtttcc aatgacataa aatactaatt
     1981 tatttggtac tacgatcaga agtcctaata ttcctgttaa tccctgtcca atggctctta
     2041 cgcctgcaag tttcatgaat gctcctccac atccagctcc taagcaagaa catacaaatg
     2101 ggatcacgga atattttaag tttgcagcaa agatggctgg ttctgaaatt cctactaatg
     2161 ttggaatgaa acttgacatt gcgatatctc tgtctttaga atgtttcttc gctaacaagt
     2221 acattccgat ggctccacca ccctgtgcaa tgatcgatac agaccagatt ccctgaatgt
     2281 agttaaatcc tgtcgtagcg atcagatttg cttcaattcc ctgaatcgcc tgatgtgttc
     2341 ctgtaactac taatggctgt aataacgctg caaagacaaa tgctccaaat gctccgaaac
     2401 tgttgtataa aacatcgatc actgctgcta atccgttacc gatcatattt ccaactggtc
     2461 cgaatactgt aaataatgct acgtttgcaa agaaaatagt aattgttggt acgaatacaa
     2521 atgataccac agctggtaca tatttctgtg caatcttttc tactttagac ataaaccatg
     2581 cggttaagat tgctgggaat actccaccct ggaatgcaac ttttgcaatt gctaatggtc
     2641 cgaaatgcca gtaacttgcg gcagatgggt ctaatacgaa tgtattacgg tttacaagat
     2701 ctggatgtac cataacgatt ccgactaaga taccaaggat cggattccca ccgaatcgtt
     2761 tgacagcact atacattaca aggattggca gataattaaa tgttgttgcc atgatcgcta
     2821 agaattctgc aatatctgca acaagtttgc tctgatctgc taaggatccc tctaatccaa
     2881 ataatccatt ggctgtcaat aatgctttga caccagtcag gatcgctgct gcaacgaatg
     2941 ctggaatgat cggaataaag atatcagaga atactttgaa tccctgctgt aattttccca
     3001 tcttgctcat tccttctgcc ttggcatcag actgagacat ttctttgaca cctgttaatt
     3061 caataaatgc atcatagaca ttgttaactg ttcctgttcc gaaaatgatc atcagctgcc
     3121 cactattaaa taagacacct ttaacacctt cgatattctc taatgcttta cggtcatatt
     3181 ttgtttcatc atttaaaatc aggcgaagtc ttgtcacaca atgtgtcgca cctttgatgt
     3241 tactgatccc accaacgtga tctaccacat cttgtgatat ttttttgtaa tccatctata
     3301 cttgtctcct ttatttctct ctttttattt gtttattttt gttgctattt ttgtgttttt
     3361 tgcttctagt gtaccatctg tcactgtcag tgccaatcca tttcctggat gatcacataa
     3421 tcttgcattt aatgccgttc cattaatata taatgttgaa atatcctggt ctatgatcag
     3481 acagatttca tattctttgt ctgcttccaa tttgatcgga cgctcaagtc caatgttaaa
     3541 gcactgatac catggataat ttggacattt gttaaagaca actttatttt cttccacaaa
     3601 gaatctgtat tcatatgaaa cttctgtttc ttcatctttg taaaatctga tagaaaattc
     3661 tttcgttcct tcttcaaatt ttacagtggt tttgaatgct gcgatgcttc ctgtatcttc
     3721 acaaagaagt gtttcttctt ttgtgtcaat cgttttcata caagggttaa ataaatcttt
     3781 ccaaccatcg aatgcttcca tcatctggtc taccggtttt accccaagtg ttccatcttc
     3841 tttctggaat acttcgtgtg gcacgaaagt tcctccccac aagtatgcgt tcttgtcatc
     3901 gttatcgatt cttgttggca cccatccaaa aagtactctt cgttctccat caaatgctgt
     3961 acgtcctgca tagtatgctc taccgtcaaa tgcatcatca aatggtgctt cccaaggccc
     4021 gtataggttc ttgcttcttc tgtaatggat tttattaccc tcactgtatt ctgagaatac
     4081 aagataccac cagtctccca tcttaaagat ctctggcatc tcaaacattg tgtagattcc
     4141 tggtgcccag aaatctcctt taaattccca tttctttaaa tcttttgatg tgaaatgtac
     4201 gagacgtcct gtctgttttc tcttatcttc tccctttctt gctccaagga tcaacagata
     4261 ttcttctctt tcctcatccc ataggacaaa tggatctctc cagtttctct tatcatatcc
     4321 ttcctgtgga ggaatctcta atgcttcttt cgatttctcc catgtgatcc catctttgct
     4381 tgtagcgtga agcaatgtct gtgatgtctt tcctgccttt aagaattctc tattatatcc
     4441 tgtataaaat gcatgaaatc ctgcctctgt ttcaaaaaca cttcctgcat agatgaactg
     4501 atctgcttct tcatccgttc cacgctttaa gctttctcca tagtctttgt aatctacaaa
     4561 atcttttgtt gttgctaatg cccaaccgaa tggttctcca aaaggtcctg gatttcttgt
     4621 atctctctga tgatatacat aaaactgacc ttcttttcca taaggcataa tatctcctac
     4681 ccaaatacct tctggctgat aatataactg atacataatt taatcctttc tttgatttac
     4741 cttgttgctt tctatggctt tattatatgt caatcgtttt catatgtcaa tcgttaccat
     4801 attaattttt gcacaaaata tttgggggat ttttggtagt tttgcataaa acttatctta
     4861 ttttgtacgt tctagcatat ttttaggcat caaaaaagga agagtagtat tttctcttcc
     4921 ttttttattc tctgttatgt tgtctgtcct tccagtaatt ttactggaag atccatacag
     4981 ctttctgctg tttttccttc aatcagatcg atcagcttat ccaccgctac ctttgcaatc
     5041 tgtttaactg gctgctggat cgttgtcagt tctggaacaa atcctaaaat ctgctttgtt
     5101 ccatctactc cgatgatctt tagatctttc ggaatattga ttcctttttg ccttgcgaca
     5161 tgaagtgcga tcactgcaag cgaatcatct cctgcaaaaa taccgtcaat ctcagggtgt
     5221 gcatttaaca tatcataaat aagtttttgc ttctccacat tctccatgat gaacggtact
     5281 tctaaaatca tctgaggaag ccctactttc tccatctctt gaatataagc ctgacttcga
     5341 ttgttacctg gcatctttaa tttactgttt cctcggatac ataaaatgtg ctgacatcct
     5401 ttatcgatca aatgtctggt tgccatctgc cctgctgcat agttatcaca tgaaacgact
     5461 ggaatatgtt ctccaagata acggtcaatt cctacgactg gcatctttaa cttggagtat
     5521 gtctctacga ctgcgttatg tgttccaacg atcattccat ctacctgatt gcgtcttagc
     5581 atatcaatat aggctttctc tgtatccatc tgatttttgc tgttacacag aagcatctta
     5641 taatttttct gtgataaata atattctatc tgttctacaa cttctccatg aaatggtgat
     5701 gttacagtag gaacgataac cccgacaaaa tacgttctgt tatttaacaa cgctctggca
     5761 atctcatttg gataataatt gatctcttcc atagcatcat atactttttt tcttgtttct
     5821 tcgctgatgt atcctcggtt atttaatacc ctggatactg ttgtgggaga aactcccgct
     5881 acttttgcca catcttttat tccaggtttc at
//

