CAZyme Information: MGYG000000287_01354

Basic Information

• Species: Robinsoniella sp900539655
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Robinsoniella; Robinsoniella sp900539655
• CAZyme ID: MGYG000000287_01354
• CAZy Family: GH2
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
833	MGYG000000287_12|CGC2	97957.11	5.4262

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000287	7249439	MAG	Sweden	Europe

• Gene Location: Start: 61154; End: 63655 Strand: -

Full Sequence      

MIRLELNHGWKMRPLGEAFQEAVVPGSVYTDLFRNGNMEDPFFKDNEIRAKKLMNKDYEY
MCRFEFHPEAFEDCERILLHFDGLDTLADIYVNSRKAGEAFSMHRTWEYEVQEMLKPGEN
ELRVVFHSPLKFLSEAYKKYGNIGNEDTVEGFMHLRKAHYMFGWDWGACLPDAGIFRPVS
LIGIRKARIDNVRIHQEHEAGEVLLTFYVEAELNERNHHFHDMNRTADKNLVRALPSDTE
GMHIKILITDPDGRILCEERGTDTFNVKQTRLWWPNGIGEQPLYEIKVLLYHKDVVLDIW
QKRIGLRTMTVRRDKDRFGESFAHEINGRTIFAMGADYIPEDHLVGRTNRQRTYRLLEQC
RLANFNSVRIWGGGYYPEDWFFDACDEMGLIVWEDFMFACSVYELTPEFEANIREEFITN
IKRIRHHASLGLWCGNNEMEMFVEQGCWVTKPSEVRDYLFMYEKIIPEVLKEHDPDTFYW
PASPSSGGSFDKPNDPDRGDVHYWEVWHGNKPFSEYRKYYFRYISEFGFQSIPSLKTLEE
ITDDPKDLNLFSYVCEKHERNRGGNGKIVKYMQQMYLYPTSFSTLIYASQLLQADGIRYG
VEHFRRNRGRCMGTVFWQLNDCWPVISWSAIDSSGRWKALLYYAKRFFAPCMISCEEESW
MTQEADMNRQHFHFDKLIRLNVANETLAERTVKVRWAMRNSDGSVIRLMEDTIQVKALSA
VWLDRIELPEIDCMNEYVSYEAEENGVVISEGTVIFTYPKYFRFRDPKLALRIEGNEIVV
SADAYAKSVEILNEDEDLVLSDNYFDMNAGERRVTVISGRISGLRVRSVYDIH

Enzyme Prediction     

No EC number prediction in MGYG000000287_01354.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	4	692	4.3e-98	0.6595744680851063

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	1.80e-89	6	806	15	799	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	5.10e-14	68	438	120	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam17753	Ig_mannosidase	1.58e-12	766	831	1	78	Ig-fold domain. This Ig-like fold domain is found in mannosidase enzymes.
pfam17786	Mannosidase_ig	7.04e-10	678	761	3	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.
PRK10150	PRK10150	5.00e-09	9	329	33	308	beta-D-glucuronidase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ANU78706.2	0.0	10	832	40	848
ASU31771.1	0.0	10	832	40	848
QQQ95478.1	0.0	12	832	1	807
QJU17896.1	0.0	12	832	1	807
QBE96379.1	0.0	2	832	26	840

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VJX_A	4.89e-139	6	818	11	817	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	5.15e-139	6	818	13	819	Structureof a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	5.29e-139	6	818	13	819	ChainA, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	1.39e-138	6	818	11	817	Structureof the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]
6BYE_A	1.53e-137	1	832	3	847	Crystalstructure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306],6BYE_B Crystal structure of the GH2 exo-beta-mannanase from Xanthomonas axonopodis pv. citri in complex with mannose [Xanthomonas citri pv. citri str. 306]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q4WAH4	9.84e-107	6	658	9	663	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
A1D911	2.67e-106	6	658	9	663	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1
B0YBU9	3.73e-106	6	658	9	663	Beta-mannosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndB PE=3 SV=1
A1CGA8	2.75e-105	3	816	6	828	Beta-mannosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndB PE=3 SV=1
Q0CCA0	3.66e-105	6	686	9	690	Beta-mannosidase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=mndB PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000059	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000000287_01354.