CAZyme Information: MGYG000000729_01209

Basic Information

• Species: F23-B02 sp900545805
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Oscillospiraceae; F23-B02; F23-B02 sp900545805
• CAZyme ID: MGYG000000729_01209
• CAZy Family: GT84
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2393	MGYG000000729_8|CGC1	264228.92	6.9252

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000000729	1856951	MAG	Kazakhstan	Asia

• Gene Location: Start: 37560; End: 44741 Strand: +

Full Sequence      

MDAQRKNTVFEHAASCARLNKCISTASSGKLRTLLRQSCREINAVYRLVESRFASAASMP
DSCRWLLDNRYLVIRQARAVMPELRRQRRLRSGSEGPLLAAACRDMISVCEDSIGEGNIL
EYLSGFQSVSPLPQDELYLLPAVLITQLISAIAELCAELESSAEPDTLSARFAALFGSVR
LVSELDMHSLLERADAVEKVLRADPSGVYPRMDEQSRAEYRLRLSRLAKRMGLEEHVFAK
QLVERCRHRSGTERHVGYWLYKRPNEERRAALYIGINILFTLFLSLLCGFLSHSFMAALL
LLLPLSELTKSLLDYIILLCVPPRRLPRLELASGVPDEGRTVCVISALLTDGDSGPRLAE
LLEQYRLANRDCGKNLLMGILADLREADIEEMPGDEAIISAAARAVDKLNEKYGGGFYLF
TRRRTLDPGRGKFSGFERKRGALLALAGLICGRKSELKTVSGDTELLRSAQYILTLDGDT
VPSPGSVCELIGAMLHPMNTPMLDRERGLVTSGHGIIHPRMCTSLASAGATDFARIFSGG
GGLEPYGALCGEVSMDLFDRGGFSGKGIIDAEALLICSQKHIPDGKVLSHDAIEGAYLRG
GYMSDVEFSDSFPVSPVAYFRRSHRWIRGDWQNAGWIFRKEAGLPDAERWRLFDSLRRSL
VAPATFAAIFAGLLLAHRGVILAAWAALIALTAGLLISFTELASDKPEALKAKYHSRTLG
GIGASIVQTAFRLWLLPYEAWISLSAIVTALWRMLISRKSLLEWETSAQSGSKRLSAAAY
FKSMWPAPVSGLCLMIFSIGIFAKAAGLMWLFAPIAAFALALPAKKEKEPTAQERSYLLG
CAKDIWSYFDTFCTEQDNYLPPDNFQEQPPVGIAHRTSPTNIGLALCSAMCAQELGITDL
TRVVSFIASMLGTMEKLQRYSGHFCNWYDTRTLRALEPRYLSAVDCGNLCACLITLQNWL
LGKGFDALADRVQTLVSDMDFSIFYSYRRGLMHIGIDLEKGKASPGLYDLMASEARLTSY
TAVSKGDVPRRHWRRLSRAMRSSGGYRGMVSWTGTMFEYLMPELFLPLTRDSLLFETAKF
CVHVQKRRRSRGGLWGISESAFYSLDPALNYRYKAHGCAQLALKRGQNAELVIAPYASFL
VLCVDPDAAAENLHRLERSGLRARYGFIEAIDFTSSRCRSAEGEKVRCFMAHHLGMSMLA
IANYLIGGLVQREFMRSPDMAAYRQLLQERVPLGAPVLRISETIEKSPAPVSLRWEKRGG
GINYAQPECALLSNGAYNIMLTESGISSASCNDMLIYRTPFKQLGEGHGPGLKLVIGGRE
RSLLPEAGENAAFMWEFSEIYASFSAVFDDFTSQCGIAAAGAENGEIRTVSICAKSDIES
AELIFSFEAVMADALDYVNHPAYWRLGIEARRDQSCLMLHRLPRGRQKEMWLCLACDEPM
TVKTGGTADDGFLSYPTVEAAVSVSLKSGKQLEARFALCVAGSPEEAYSGAQHMLVIGPS
EYGAMVPACASVMHMSAREVEAAMATLRALRFISAKTPFPAKSSLWRFGLSGDLPIICCD
GGQDTESVTKQFCLLRSCGVYADLVFLTDEGGEYRRPVYSKVRDILAAHGLEALIGTYAG
VRMLPVQETSLIENAAAFIVGAESKKRSFNSTPRPSKPLRTGGTAPRHEYGNDGSFVFYV
NRTLPSRVWSSMLTNGSFGYIAADSGMGNMWLKNARELRLTPWVNDPLAIEGAESLEYVT
GDRRYSLFAGEDGAACRVTFGLGFAVWEKSLGSCASRCTAFVLPDLDARVFIIELFGEIS
GRLDWKCELLLSDNDNDRTSVGCEYSDTMFTASSSRSAIRNLHFRAACSNPALGRTCDYL
SWLHGDFDNRKSELSFPVFAAQYEARAVTVLVCGCEDADTILRLCEPDAAFAALEKTKAH
WAGLCRRFTLSGCGSADKYMSGWAVYQTLACRIMGRCSVYQSGGAFGFRDQLQDAVNLIL
LDPEIAKQQLLACCRHQYLEGDVMHWWHSAGGADKGVRTRCSDDLLWLVWALCEYVEKTG
DTDICGRLECYVNSKPLEADEDDRYETPLRSDCTESVLMHAQRAVDCCISRGTGAHGLLC
FGSGDWNDGMNNIFGESVWLSWFFACCVKRFADLLAGLCRPDADKYRAFAADIGRAADRA
WNGQWYLRGYWPDGEELGASQNECCRIDSIAQSWACFCSEASNAKAGTALDAALRLLFDR
ENGIVKLFDPPFFGGVRDPGYIRSYGPGFRENGGQYTHAALWLAQACFKRGRANDGMAIL
ESLLPESHDLNRYMAEPFVIPADVYTCPGHAGEAGWTWYTGSSGWYFRVFTEELLGLKPR
HGMVCIRPCLPDDFPDCRVTLKTKAGKAHDIVIGHGGIWLDGAIYDGKGIPYI

Enzyme Prediction     

EC	2.4.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH94	1541	2391	3.4e-164	0.8561776061776062
GT84	1007	1218	2.2e-76	0.9906976744186047

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3459	COG3459	2.12e-129	1689	2392	296	1040	Cellobiose phosphorylase [Carbohydrate transport and metabolism].
pfam17167	Glyco_hydro_36	7.43e-85	1920	2338	1	425	Glycosyl hydrolase 36 superfamily, catalytic domain. This is the catalytic region of the superfamily of enzymes referred to as GH36. UniProtKB:Q76IQ9 is a chitobiose phosphorylase that catalyzes the reversible phosphorolysis of chitobiose into alpha-GlcNAc-1-phosphate and GlcNAc with inversion of the anomeric configuration. The full-length enzyme comprises a beta sandwich domain and an (alpha/alpha)(6) barrel domain. The alpha-helical barrel component of the domain, this family, is the catalytic region.
pfam10091	Glycoamylase	5.71e-21	1008	1218	3	215	Putative glucoamylase. The structure of UniProt:Q5LIB7 has an alpha/alpha toroid fold and is similar structurally to a number of glucoamylases. Most of these structural homologs are glucoamylases, involved in breaking down complex sugars (e.g. starch). The biologically relevant state is likely to be monomeric. The putative active site is located at the centre of the toroid with a well defined large cavity.
cd11753	GH94N_ChvB_NdvB_2_like	1.94e-19	1211	1432	2	234	Second GH94N domain of cyclic beta 1-2 glucan synthetase and similar domains. The glycoside hydrolase family 94 (previously known as glycosyltransferase family 36) includes cyclic beta 1-2 glucan synthetase (EC:2.4.1.20) or ChvB (encoded by the chromosomal chvB virulence gene). This second of two tandemly repeated GH94-N-terminal-like domains has not been characterized functionally. Some beta 1-2 glucan synthetases are annotated as NdvB (nodule development B) gene products, glycosyltransferases required for the synthesis of cyclic beta-(1,2)-glucans, which play a role in interactions between bacteria and plants.
cd11756	GH94N_ChvB_NdvB_1_like	5.71e-10	1672	1795	14	140	First GH94N domain of cyclic beta 1-2 glucan synthetase and similar domains. The glycoside hydrolase family 94 (previously known as glycosyltransferase family 36) includes cyclic beta 1-2 glucan synthetase (EC:2.4.1.20) or ChvB (encoded by the chromosomal chvB virulence gene). This first of two tandemly repeated GH94-N-terminal-like domains has not been characterized functionally. Some beta 1-2 glucan synthetases are annotated as NdvB (nodule development B) gene products, glycosyltransferases required for the synthesis of cyclic beta-(1,2)-glucans, which play a role in interactions between bacteria and plants.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QBB66089.1	0.0	32	2391	34	2385
ALP93881.1	0.0	32	2092	34	2084
CDZ23197.1	0.0	40	2393	41	2484
AVQ95160.1	0.0	1	2377	11	2524
AYF37850.1	0.0	1	2377	11	2524

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1V7V_A	3.46e-40	1908	2369	287	758	Crystalstructure of Vibrio proteolyticus chitobiose phosphorylase [Vibrio proteolyticus],1V7W_A Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc [Vibrio proteolyticus],1V7X_A Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc and sulfate [Vibrio proteolyticus]
2CQS_A	3.03e-35	1912	2392	326	829	CrystalStructure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Ammonium Sulfate [Cellulomonas gilvus],2CQS_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Ammonium Sulfate [Cellulomonas gilvus],2CQT_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Sodium/Potassium Phosphate [Cellulomonas gilvus],2CQT_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Sodium/Potassium Phosphate [Cellulomonas gilvus],3QFY_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and Isofagomine [Cellulomonas gilvus],3QFY_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and Isofagomine [Cellulomonas gilvus],3QFZ_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QFZ_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QG0_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Phosphate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QG0_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Phosphate and 1-Deoxynojirimycin [Cellulomonas gilvus]
3AFJ_A	4.00e-35	1912	2392	326	829	CrystalStructure of Cellvibrio gilvus Cellobiose Phosphorylase triple mutant [Cellulomonas gilvus ATCC 13127],3AFJ_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase triple mutant [Cellulomonas gilvus ATCC 13127]
3S4C_A	1.95e-34	1906	2392	303	809	Lactosephosphorylase in complex with sulfate [Cellulomonas uda],3S4D_A Lactose phosphorylase in a ternary complex with cellobiose and sulfate [Cellulomonas uda]
3ACT_A	2.11e-34	1912	2392	326	829	CrystalStructure of Cellvibrio gilvus Cellobiose Phosphorylase Histidine mutant [Cellulomonas gilvus ATCC 13127],3ACT_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Histidine mutant [Cellulomonas gilvus ATCC 13127]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P20471	2.72e-263	36	2373	63	2805	Cyclic beta-(1,2)-glucan synthase NdvB OS=Rhizobium meliloti (strain 1021) OX=266834 GN=ndvB PE=1 SV=2
Q76IQ9	1.82e-39	1908	2369	287	758	N,N'-diacetylchitobiose phosphorylase OS=Vibrio proteolyticus OX=671 GN=chbP PE=1 SV=1
Q9F8X1	8.36e-36	1752	2378	94	767	N,N'-diacetylchitobiose phosphorylase OS=Vibrio furnissii OX=29494 GN=chbP PE=1 SV=1
B9K7M6	3.13e-33	1913	2391	301	798	Cellobiose phosphorylase OS=Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NBRC 107923 / NS-E) OX=309803 GN=cbpA PE=1 SV=1
Q8P3J4	2.25e-24	1969	2367	362	753	Cellobionic acid phosphorylase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=XCC4077 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000064	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
271	293
659	676
681	703
730	752
791	813