CAZyme Information: MGYG000001063_01406

Basic Information

• Species: Robinsoniella sp900540475
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Robinsoniella; Robinsoniella sp900540475
• CAZyme ID: MGYG000001063_01406
• CAZy Family: GH78
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
595	MGYG000001063_35|CGC1	70441.56	4.7263

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001063	7637037	MAG	Sweden	Europe

• Gene Location: Start: 7635; End: 9422 Strand: +

Full Sequence      

MKENYRDIKAQNDGNLQSPRWIWYPGDFEIYHALKVNTRREERNIMWPAFWRLDDCYHNV
RFRKKAFFEKEFSICAYANGKGYLDVDGKKYPFGEAVLLPEGEHELLAVVYHTEGLPALY
AEGGNAASNGSWEVNMYSDEWLPAGFNELYFDKGRTPDTFPFSYKETRPVSAERLEAGML
YDFGREIFASVVVRNRGKEGELGLYYGESRREALDKEQCYLYEKTGYVQDEMVFKNRAFR
YLLVENQNEMEVTAIEEYLPLEPVKRFQCQDELLNKIWEISEYTFHLNSREFFLDGIKRD
RWIWSGDAYQAYKINQYLYQDKDLNRRTILALRGKDPVECHINTITDYSFFWIMSVYEYY
QGYKDKEFLKMIYPKMKSLMEFCIERTDENGFIVKKCSDWVFIDWADIDKDGAVCAEQIL
FAKSLEAILMSAEILGIEESAYRERLEILCRDIDTHFWDEEQGAYISSFSSGKKQVTRHA
NIFAVLYDFADEDKQHKIIKNVIHNDKIPQIVTPYFKFFELDMMGKIGERQYVWDEIRDY
WGGMIEKGATAFWETYDPDEDEKERLAMYGDPYGKSLCHAWGASPIYLLGRYFSE

Enzyme Prediction     

EC	3.2.1.40

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH78	178	592	3e-88	0.8551587301587301

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam17389	Bac_rhamnosid6H	1.08e-27	267	592	6	339	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam06202	GDE_C	2.05e-05	343	515	81	267	Amylo-alpha-1,6-glucosidase. This family includes human glycogen branching enzyme AGL. This enzyme contains a number of distinct catalytic activities. It has been shown for the yeast homolog GDB1 that mutations in this region disrupt the enzymes Amylo-alpha-1,6-glucosidase (EC:3.2.1.33).
pfam04685	DUF608	0.002	312	447	84	200	Glycosyl-hydrolase family 116, catalytic region. This represents a family of archaeal, bacterial and eukaryotic glycosyl hydrolases, that belong to superfamily GH116. The primary catabolic pathway for glucosylceramide is catalysis by the lysosomal enzyme glucocerebrosidase. In higher eukaryotes, glucosylceramide is the precursor of glycosphingolipids, a complex group of ubiquitous membrane lipids. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia. The catalytic nucleophile, identified in UniProtKB:Q97YG8_SULSO, is a glutamine-335, with the likely acid/base at Asp-442 and the aspartates at Asp-406 and Asp-458 residues also playing a role in the catalysis of glucosides and xylosides that are beta-bound to hydrophobic groups. The family is defined as GH116, which presently includes enzymes with beta-glucosidase, EC:3.2.1.21, beta-xylosidase, EC:3.2.1.37, and glucocerebrosidase EC:3.2.1.45 activity.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QYR22424.1	6.18e-240	17	593	3	581
QKS45153.1	6.18e-239	20	593	8	583
AKZ49187.1	2.81e-198	20	594	8	580
ASV94656.1	2.81e-198	20	594	8	580
AKX85536.1	2.81e-198	20	594	8	580

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3CIH_A	1.65e-163	21	593	20	636	Crystalstructure of a putative alpha-rhamnosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
4XHC_A	1.12e-36	238	592	172	541	ChainA, Alpha-L-rhamnosidase [Klebsiella oxytoca],4XHC_B Chain B, Alpha-L-rhamnosidase [Klebsiella oxytoca]
2OKX_A	2.40e-36	237	590	503	873	Crystalstructure of GH78 family rhamnosidase of Bacillus SP. GL1 AT 1.9 A [Bacillus sp. GL1],2OKX_B Crystal structure of GH78 family rhamnosidase of Bacillus SP. GL1 AT 1.9 A [Bacillus sp. GL1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KPL4	2.31e-07	180	440	385	681	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22170 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000062	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001063_01406.