CAZyme Information: MGYG000002364_01395

Basic Information

• Species: Aliarcobacter butzleri
• Lineage: Bacteria; Campylobacterota; Campylobacteria; Campylobacterales; Arcobacteraceae; Aliarcobacter; Aliarcobacter butzleri
• CAZyme ID: MGYG000002364_01395
• CAZy Family: GH102
• CAZyme Description: Membrane-bound lytic murein transglycosylase A

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
363	MGYG000002364_54|CGC1	41430.67	9.5583

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002364	2233660	Isolate	Canada	North America

• Gene Location: Start: 12870; End: 13961 Strand: -

Full Sequence      

MKSLILILFLTFLVTGCSTKKEVFEEEKKEVKNRMEPVSFAEIKGFFEDDLNYALEVFKK
DCQKSKKYEQFKSVCQKAQYETDGRKFFIVNFQPYKLYDSNSHDEGTITGYYEPLLYGSL
KKSARYKYPVYKIPKNLITSDNANLEGYKSRGKLVGKKIVPYDTRKEIESNPNNPNLEVI
AYVDDKFDLFFLHIQGSGKIQLDNGKLINVAYAEQNGRAYTSIGGYLISQGLMTKDEMSV
QSMKKFFANNPSKMDYIFNLNESYIFFKISNQGATGALNTVLTPKRNLAVDKSYIPLGTP
IFLNTQNPVSKQPINQMMVAADVGGAIKGEIRADFFWGFGKDAFEYAGRMKEKGKMYILL
PKN

Enzyme Prediction     

No EC number prediction in MGYG000002364_01395.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH102	117	268	6.7e-52	0.9872611464968153

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam03562	MltA	4.33e-92	46	268	1	231	MltA specific insert domain. This beta barrel domain is found inserted in the MltA a murein degrading transglycosylase enzyme. This domain may be involved in peptidoglycan binding.
COG2821	MltA	5.24e-88	76	363	72	367	Membrane-bound lytic murein transglycosylase [Cell wall/membrane/envelope biogenesis].
cd14668	mlta_B	1.69e-68	116	269	1	158	Domain B insert of mltA_like lytic transglycosylases. Escherichia coli MltA is a membrane-bound lytic transglycosylase comprised of two domains separated by a large groove, where the peptidoglycan strand binds. Domain A is made up of an N-terminal and a C-terminal portion, which correspond to the 3D domain, named for 3 conserved aspartate residues. Domain B is inserted within the linear sequence of domain A. MltA is distinct from other bacterial lytic transglycosylases (LTs), which are similar to each other. Escherichia coli peptidoglycan lytic transglycosylase (LT) initiates cell wall recycling in response to damage, during bacterial fission, and cleaves peptidoglycan (PG) to create functional spaces in its wall. PG chains (also known as murein), the major components of the bacterial cell wall, are comprised of alternating beta-1-4-linked N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), and lytic transglycosylases cleave this beta-1-4 bond. Typically, peptidoglycan lytic transglycosylases (LT) are exolytic, releasing Metabolite 1 (GlcNAc-anhMurNAc-L-Ala-D-Glu-m-Dap-D-Ala-D-Ala) from the ends of the PG strands. In contrast, MltE is endolytic , cleaving in the middle of PG strands, with further processing to Metabolite 1 accomplished by other LTs. In E. coli, there are six membrane-bound LTs: MltA-MltF and soluble Slt70. Slt35 is a soluble fragment cleaved from MltB. Bacterial LTs are classified in 4 families: Family 1 includes slt70 MltC-MltF, Family 2 includes MltA, Family 3 includes MltB, and Family 4 of bacteriophage origin. While most of the LT family members are similar in structure and sequence with a lysozyme-like fold, Family 2 (including mltA) is distinct.
smart00925	MltA	6.63e-68	115	268	1	153	MltA specific insert domain. This beta barrel domain is found inserted in the MltA a murein degrading transglycosylase enzyme. This domain may be involved in peptidoglycan binding.
PRK11162	mltA	9.94e-40	108	359	118	354	murein transglycosylase A; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BAK71706.1	2.25e-265	1	363	1	363
QDM02078.1	2.25e-265	1	363	1	363
AGR78368.1	9.17e-265	1	363	1	363
SNV34410.1	9.88e-262	1	363	1	362
ABV68408.1	9.88e-262	1	363	1	362

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6QK4_B	1.08e-64	34	363	24	348	Lytictransglycosylase, LtgG, of Burkholderia pseudomallei. [Burkholderia pseudomallei]
2G5D_A	1.81e-61	50	361	57	413	Crystalstructure of MltA from Neisseria gonorrhoeae Monoclinic form [Neisseria gonorrhoeae FA 1090]
7ESJ_A	5.12e-60	85	362	79	360	ChainA, membrane-bound lytic murein transglycosylase A [Acinetobacter baumannii],7ESJ_B Chain B, membrane-bound lytic murein transglycosylase A [Acinetobacter baumannii]
2G6G_A	7.62e-60	50	361	57	413	Crystalstructure of MltA from Neisseria gonorrhoeae [Neisseria gonorrhoeae FA 1090]
2PNW_A	2.44e-37	85	362	76	364	Crystalstructure of membrane-bound lytic murein transglycosylase from Agrobacterium tumefaciens [Agrobacterium fabrum str. C58]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P57531	7.32e-26	108	362	118	359	Membrane-bound lytic murein transglycosylase A homolog OS=Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) OX=107806 GN=mltA PE=3 SV=1
P0A935	1.51e-25	83	359	93	353	Membrane-bound lytic murein transglycosylase A OS=Escherichia coli (strain K12) OX=83333 GN=mltA PE=1 SV=1
P0A936	1.51e-25	83	359	93	353	Membrane-bound lytic murein transglycosylase A OS=Escherichia coli O157:H7 OX=83334 GN=mltA PE=3 SV=1
Q9KPQ4	2.54e-23	109	359	114	349	Membrane-bound lytic murein transglycosylase A OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=mltA PE=3 SV=1
Q8K9A7	3.07e-22	108	359	126	364	Membrane-bound lytic murein transglycosylase A homolog OS=Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) OX=198804 GN=mltA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000000	0.000000	1.000064	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000002364_01395.