CAZyme Information: MGYG000002886_03235

Basic Information

• Species: Stenotrophomonas maltophilia
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Stenotrophomonas; Stenotrophomonas maltophilia
• CAZyme ID: MGYG000002886_03235
• CAZy Family: GT2
• CAZyme Description: Cellulose synthase catalytic subunit [UDP-forming]

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
741		83738.2	9.9209

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000002886	4276654	MAG	United States	North America

• Gene Location: Start: 138; End: 2363 Strand: -

Full Sequence      

MAAWFGRKVGSLSPRSRHVLTLLVGLATLGLAALCITQPFTYLAQFVFVLLLWVIALLVR
RMQGRYATLVLVVLSITVSCRYLWWRYTATLNWNSTFDLVCGVVLLAAETYSWLVLMLGY
VQVAWPLRRRPAPLPADIHQWPTVDVLIPTYNEDLALVRHTVYAAMGLDWPADKLRIHIL
DDGKREDFREFAERAGVNYITRSNNHHAKAGNLNHALTLIDGELVAIFDSDHLPVRSFLQ
ITCGWFLRDPKLALVQTPHHFFSADPFERNLQVFRSDPNEGELFYGLVQDGNDLWNAAFF
CGSCAVLRRKAIDAIGGFATETVTEDAHTALRLHRKGWNSAYLRIPQAAGLATDSLGAHV
NQRIRWARGMVQIFRIDNPLLGKGLSLFQRFCYANAMLHFLAGIPRLVFLTAPLAFLLLH
VYIIYAPALAILLFVVPHMAHASLTNARIQGKYRRPFWGEVYETVLAWYIARPTTVALFS
PGRGKFNVTDKGGTQAGDRFDWRVAQPYLVLALLNVLGLCFAVWRFMHGPLDERGTVVVS
SLWVLYNLLIIGGALAVAAEVQQVRRTHRVTTHLPMALQVPDGRRLRGVLQDYSNDGVGV
ELGEDAQLAEGQRVQVLLGRGRREFAFPARVQRTVGQRLGLLLLFEDERQRVEFAQCTFA
RADAWLDWHAGYQPKSLPRSLWSVLVLGWRGYRRMGDFAPFDLDRPAHWNRRLLRWLASF
APQRPLPSHPADPAADPGLRP

Enzyme Prediction     

EC	2.4.1.12

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	145	315	3.9e-35	0.9882352941176471

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK11498	bcsA	0.0	7	734	125	852	cellulose synthase catalytic subunit; Provisional
cd06421	CESA_CelA_like	2.74e-119	142	374	1	234	CESA_CelA_like are involved in the elongation of the glucan chain of cellulose. Family of proteins related to Agrobacterium tumefaciens CelA and Gluconacetobacter xylinus BscA. These proteins are involved in the elongation of the glucan chain of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues. They are putative catalytic subunit of cellulose synthase, which is a glycosyltransferase using UDP-glucose as the substrate. The catalytic subunit is an integral membrane protein with 6 transmembrane segments and it is postulated that the protein is anchored in the membrane at the N-terminal end.
COG1215	BcsA	7.01e-61	102	561	13	437	Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1,6-N-acetylglucosamine synthase [Cell motility].
cd06437	CESA_CaSu_A2	1.42e-37	142	369	1	231	Cellulose synthase catalytic subunit A2 (CESA2) is a catalytic subunit or a catalytic subunit substitute of the cellulose synthase complex. Cellulose synthase (CESA) catalyzes the polymerization reaction of cellulose using UDP-glucose as the substrate. Cellulose is an aggregate of unbranched polymers of beta-1,4-linked glucose residues, which is an abundant polysaccharide produced by plants and in varying degrees by several other organisms including algae, bacteria, fungi, and even some animals. Genomes from higher plants harbor multiple CESA genes. There are ten in Arabidopsis. At least three different CESA proteins are required to form a functional complex. In Arabidopsis, CESA1, 3 and 6 and CESA4, 7 and 8, are required for cellulose biosynthesis during primary and secondary cell wall formation. CESA2 is very closely related to CESA6 and is viewed as a prime substitute for CESA6. They functionally compensate each other. The cesa2 and cesa6 double mutant plants were significantly smaller, while the single mutant plants were almost normal.
cd06435	CESA_NdvC_like	1.60e-29	148	375	4	233	NdvC_like proteins in this family are putative bacterial beta-(1,6)-glucosyltransferase. NdvC_like proteins in this family are putative bacterial beta-(1,6)-glucosyltransferase. Bradyrhizobium japonicum synthesizes periplasmic cyclic beta-(1,3),beta-(1,6)-D-glucans during growth under hypoosmotic conditions. Two genes (ndvB, ndvC) are involved in the beta-(1, 3), beta-(1,6)-glucan synthesis. The ndvC mutant strain resulted in synthesis of altered cyclic beta-glucans composed almost entirely of beta-(1, 3)-glycosyl linkages. The periplasmic cyclic beta-(1,3),beta-(1,6)-D-glucans function for osmoregulation. The ndvC mutation also affects the ability of the bacteria to establish a successful symbiotic interaction with host plant. Thus, the beta-glucans may function as suppressors of a host defense response.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AYZ68771.1	0.0	1	741	134	874
VEE53091.1	0.0	1	741	134	874
ARQ88606.1	0.0	1	741	134	874
QGL87319.1	0.0	1	741	134	874
QGL62300.1	0.0	3	741	136	874

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7LBY_A	0.0	7	734	138	865	ChainA, Cellulose synthase catalytic subunit [UDP-forming] [Escherichia coli K-12]
5EJ1_A	1.87e-106	81	598	67	603	ChainA, Putative cellulose synthase [Cereibacter sphaeroides 2.4.1]
4HG6_A	1.09e-105	81	598	79	615	ChainA, Cellulose Synthase Subunit A [Cereibacter sphaeroides]
4P00_A	1.11e-105	81	598	80	616	ChainA, Cellulose Synthase A subunit [Cereibacter sphaeroides 2.4.1],4P02_A Chain A, Cellulose Synthase subunit A [Cereibacter sphaeroides 2.4.1],5EIY_A Chain A, Putative cellulose synthase [Cereibacter sphaeroides 2.4.1],5EJZ_A Chain A, Putative cellulose synthase [Cereibacter sphaeroides 2.4.1]
6YV7_B	6.62e-16	129	399	29	292	MannosyltransferasePcManGT from Pyrobaculum calidifontis [Pyrobaculum calidifontis JCM 11548],6YV8_B Mannosyltransferase PcManGT from Pyrobaculum calidifontis in complex with GDP and Mn2+ [Pyrobaculum calidifontis JCM 11548],6YV9_A Mannosyltransferase PcManGT from Pyrobaculum calidifontis in complex with GDP-Man and Mn2+ [Pyrobaculum calidifontis JCM 11548]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8Z291	0.0	7	725	138	856	Cellulose synthase catalytic subunit [UDP-forming] OS=Salmonella typhi OX=90370 GN=bcsA PE=3 SV=1
Q8X5L7	0.0	7	734	138	865	Cellulose synthase catalytic subunit [UDP-forming] OS=Escherichia coli O157:H7 OX=83334 GN=bcsA PE=3 SV=2
P37653	0.0	7	734	138	865	Cellulose synthase catalytic subunit [UDP-forming] OS=Escherichia coli (strain K12) OX=83333 GN=bcsA PE=1 SV=3
Q93IN2	0.0	7	725	138	856	Cellulose synthase catalytic subunit [UDP-forming] OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bcsA PE=3 SV=1
P58931	1.17e-259	36	703	53	720	Cellulose synthase catalytic subunit [UDP-forming] OS=Pseudomonas fluorescens (strain SBW25) OX=216595 GN=bcsA PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000053	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
20	37
42	59
66	85
100	122
397	419
423	445
508	527
537	559