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CAZyme Information: MGYG000003302_00059

You are here: Home > Sequence: MGYG000003302_00059

Basic Information | Genomic context | Full Sequence | Enzyme annotations |  CAZy signature domains |  CDD domains | CAZyme hits | PDB hits | Swiss-Prot hits | SignalP and Lipop annotations | TMHMM annotations

Basic Information help

Species UBA733 sp900539595
Lineage Bacteria; Firmicutes; Bacilli; RFN20; CAG-826; UBA733; UBA733 sp900539595
CAZyme ID MGYG000003302_00059
CAZy Family GH13
CAZyme Description 1,4-alpha-glucan branching enzyme GlgB
CAZyme Property
Protein Length CGC Molecular Weight Isoelectric Point
637 MGYG000003302_1|CGC1 74192.75 6.5054
Genome Property
Genome Assembly ID Genome Size Genome Type Country Continent
MGYG000003302 1776566 MAG China Asia
Gene Location Start: 67045;  End: 68958  Strand: -

Full Sequence      Download help

Enzyme Prediction      help

EC 2.4.1.18

CAZyme Signature Domains help

Family Start End Evalue family coverage
GH13 176 463 6.1e-123 0.9933554817275747

CDD Domains      download full data without filtering help

Cdd ID Domain E-Value qStart qEnd sStart sEnd Domain Description
COG0296 GlgB 0.0 16 586 21 601
1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK12313 PRK12313 0.0 12 613 18 626
1,4-alpha-glucan branching protein GlgB.
PRK05402 PRK05402 0.0 16 617 115 726
1,4-alpha-glucan branching protein GlgB.
PRK14705 PRK14705 0.0 12 580 614 1190
glycogen branching enzyme; Provisional
cd11322 AmyAc_Glg_BE 0.0 114 498 2 400
Alpha amylase catalytic domain found in the Glycogen branching enzyme (also called 1,4-alpha-glucan branching enzyme). The glycogen branching enzyme catalyzes the third step of glycogen biosynthesis by the cleavage of an alpha-(1,4)-glucosidic linkage and the formation a new alpha-(1,6)-branch by subsequent transfer of cleaved oligosaccharide. They are part of a group called branching enzymes which catalyze the formation of alpha-1,6 branch points in either glycogen or starch. This group includes proteins from bacteria, eukaryotes, and archaea. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase.

CAZyme Hits      help

Hit ID E-Value Query Start Query End Hit Start Hit End
QOS39154.1 8.87e-240 1 612 1 603
BBH27653.1 3.85e-222 12 617 13 619
BCL56856.1 3.22e-215 12 612 13 621
QMW75175.1 1.18e-211 9 616 10 618
QPS14491.1 1.18e-211 9 616 10 618

PDB Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
5GR2_A 3.40e-168 9 580 137 741
Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GQW_A 6.78e-168 9 580 137 741
Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A 9.58e-168 9 580 137 741
Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GR1_A 9.58e-168 9 580 137 741
Crystalstructure of branching enzyme Y500A/D501A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142],5GR6_A Crystal structure of branching enzyme Y500A/D501A double mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GR0_A 1.35e-167 9 580 137 741
Crystalstructure of branching enzyme D501A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits      download full data without filtering help

Hit ID E-Value Query Start Query End Hit Start Hit End Description
Q7NL20 4.20e-177 38 587 135 703
1,4-alpha-glucan branching enzyme GlgB OS=Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) OX=251221 GN=glgB PE=3 SV=1
Q632H1 3.62e-176 4 580 5 578
1,4-alpha-glucan branching enzyme GlgB OS=Bacillus cereus (strain ZK / E33L) OX=288681 GN=glgB PE=3 SV=1
Q72YJ3 6.63e-176 4 580 13 586
1,4-alpha-glucan branching enzyme GlgB OS=Bacillus cereus (strain ATCC 10987 / NRS 248) OX=222523 GN=glgB PE=3 SV=1
B7HTX9 9.37e-176 4 580 13 586
1,4-alpha-glucan branching enzyme GlgB OS=Bacillus cereus (strain AH187) OX=405534 GN=glgB PE=3 SV=1
B9J2G8 1.88e-175 4 580 13 586
1,4-alpha-glucan branching enzyme GlgB OS=Bacillus cereus (strain Q1) OX=361100 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations help

This protein is predicted as OTHER

Other SP_Sec_SPI LIPO_Sec_SPII TAT_Tat_SPI TATLIP_Sec_SPII PILIN_Sec_SPIII
1.000040 0.000008 0.000000 0.000000 0.000000 0.000000

TMHMM  Annotations      help

There is no transmembrane helices in MGYG000003302_00059.