CAZyme Information: MGYG000003610_00730

Basic Information

• Species: HGM04593 sp900770665
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; HGM04593; HGM04593 sp900770665
• CAZyme ID: MGYG000003610_00730
• CAZy Family: GH85
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1150	MGYG000003610_28|CGC1	126856.35	4.8523

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003610	2461139	MAG	Fiji	Oceania

• Gene Location: Start: 23045; End: 26497 Strand: -

Full Sequence      

MKKFYLMLISLLCIAFSAQAQVVTNLDQLSNDKVYTIKSARAFLLYSPNCTKIASSTGKS
VGNVTFDPQDPNQQFKIENVGGKYYLYSVGAGKYVYTNGMFNANTVTPLNISQSSSATYR
WFLKIGTQGLNSQEPNQTAEGVVVDSWVTQDEGNCYSITEVTDGEGGSSTSEEIYDFAGF
GVEDLLELFNSALEKGRNYPTMEEFAAAGIQASDIAFVRSHVRKANIMSREDRVRKETYQ
NRNLFLNIPMDYGKDGGVGYPHAKFNADVFSMWQYTNLFGSWNHGFFTAPGAWVDAAHRN
GTDIMSGIAFFESWTGDGDKVFSAMITRKNSDGTYKYVKPLINILMFFGSDGINYNWEDN
SWGNSDIVAFHKALWVEAKRVGFDNYHSAIYTSTSELQAGSANIDALYGTKTAGKTHDLM
LNYQANDIAGNLGNTYNAVKNAYGEADNMYAGVWIASMDRRWTNLEGNNTSICLWGEHDQ
SRFYSFNAGDGVFDIQGNYQRLLERAMSGGNRNPATLPALSNTGNNWQTENGKLPLQTFA
GMAHWIPERSAIQGDMHFRTHFTLGNGDRYYYKGKMSHKSEWYNMGSQDIVPTYRWLRYK
SNTTNVSTDIDVNYTHLDAYTGGSCIELTGAATTEGTDIILYKTSLKAGDGAYAKLAVKT
LKDVKATNLYLIVRKAGSNNWLEFPYGDVAGKTWEEKKFALTGIAKGDVIDRIGLRVKGS
NNDYQLYVGKLELNDETYIDPAAVKDLVAEVKSETKESMNLKLHWNVDATAKTRAAWGLV
FNDEANIDHFEVLYKVGEDGKVALVGTTSQWATFVGNIEFESANDVPYIGVRSVSTDLKT
YSPIAWVEVPRGDQAKLPEREEVDTYGISQIDPNSNGIEAAREQRYVELVKTTGATQNLN
YTANAPVADGTQYADARDHLLIVQQGQSITMTLKGPNLKDGLQWCFGGGWMDLNGSGDFD
HPFPTERTAAEIAKGKTEVDPEGERIFFIGTHEKSTEALVTGYTFTFKIPEDATPGNSRL
RLVFSDAWFAGAFNPTGLTNKGFTIDFSVKIEGNNPGRSASGTRDQGEAEEPELLDGNTS
VENVAGEVSVAEGVEGAIEIANADVAWIYTADGKLVEFVQNPATVAVEAGVYIVKMQLGN
VIRNAKVLVK

Enzyme Prediction     

No EC number prediction in MGYG000003610_00730.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH85	263	571	6.8e-35	0.9523809523809523

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4724	COG4724	2.77e-32	264	736	98	552	Endo-beta-N-acetylglucosaminidase D [Carbohydrate transport and metabolism].
cd06547	GH85_ENGase	7.06e-25	262	599	19	336	Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the protein and N-acetylglucosamine 2 forms the reducing end of the released glycan. ENGase is a key enzyme in the processing of free oligosaccharides in the cytosol of eukaryotes. Oligosaccharides formed in the lumen of the endoplasmic reticulum are transported into the cytosol where they are catabolized by cytosolic ENGases and other enzymes, possibly to maximize the reutilization of the component sugars. ENGases have an eight-stranded alpha/beta barrel topology and are classified as a family 85 glycosyl hydrolase (GH85) domain. The GH85 ENGases are sequence-similar to the family 18 glycosyl hydrolases, also known as GH18 chitinases. An ENGase-like protein is also found in bacteria and is included in this alignment model.
pfam03644	Glyco_hydro_85	7.37e-10	261	567	1	291	Glycosyl hydrolase family 85. Family of endo-beta-N-acetylglucosaminidases. These enzymes work on a broad spectrum of substrates.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BCI64641.1	0.0	169	1146	32	1003
AGB28382.1	0.0	169	1150	201	1190
QUT74553.1	0.0	169	1146	28	1016
EFC71166.1	1.70e-313	169	1150	31	1006
QFQ13761.1	2.99e-310	168	1149	29	991

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3FHQ_A	3.37e-26	214	826	26	595	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_F Chain F, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]
3FHA_A	4.47e-26	214	826	26	595	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_C Chain C, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]
2VTF_A	4.59e-26	214	826	31	600	X-raycrystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae],2VTF_B X-ray crystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000223	0.999156	0.000183	0.000148	0.000138	0.000134

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003610_00730.