CAZyme Information: MGYG000003890_00287

Basic Information

• Species: Zag111 sp900551965
• Lineage: Bacteria; Cyanobacteria; Vampirovibrionia; Gastranaerophilales; Gastranaerophilaceae; Zag111; Zag111 sp900551965
• CAZyme ID: MGYG000003890_00287
• CAZy Family: GT9
• CAZyme Description: Lipopolysaccharide core heptosyltransferase RfaQ

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
331		36627.94	8.4768

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000003890	1929453	MAG	United States	North America

• Gene Location: Start: 264133; End: 265128 Strand: +

Full Sequence      

MEKKKRILAINFGGIGDEIFFLPTLISLKKEFPNSEIVLALEPRSKSVKDLTNIIDDLIL
IDVKGKNKYLEMFKLLLSARTGNFNLVVSSGGNKLISILLFLTGIKERCGYDTGKLSRML
LTHAMPLNKNQYACAMYHDLIRAVTEHNTILPEIDVEKKEKIANSVLIHPGVSRLSVQKG
CIKTVPAETWAKVIDLLVAEGKKVMLVGGPDDKECIDAILNTVRTQNFENLYGTTKSLKD
LAELISSAEKFLCSDSAPLHAAVAMHTKTYVIFGPTDDRALIPQSDDVVPIKANDNCELK
PCLWARRQTTCEKLNCLKISASQIAAAICRN

Enzyme Prediction     

No EC number prediction in MGYG000003890_00287.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT9	74	311	1e-33	0.9466666666666667

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG0859	RfaF	1.20e-44	5	301	2	305	ADP-heptose:LPS heptosyltransferase [Cell wall/membrane/envelope biogenesis].
cd03789	GT9_LPS_heptosyltransferase	1.03e-41	6	330	1	277	lipopolysaccharide heptosyltransferase and similar proteins. Lipopolysaccharide heptosyltransferase (2.4.99.B6) is involved in the biosynthesis of lipooligosaccharide (LOS). Lipopolysaccharide (LPS) is a major component of the outer membrane of gram-negative bacteria. LPS heptosyltransferase transfers heptose molecules from ADP-heptose to 3-deoxy-D-manno-octulosonic acid (KDO), a part of the inner core component of LPS. This family also contains lipopolysaccharide 1,2-N-acetylglucosaminetransferase EC 2.4.1.56 and belongs to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility.
PRK10422	PRK10422	3.73e-17	1	295	1	310	lipopolysaccharide core biosynthesis protein; Provisional
pfam01075	Glyco_transf_9	9.29e-16	72	314	1	246	Glycosyltransferase family 9 (heptosyltransferase). Members of this family belong to glycosyltransferase family 9. Lipopolysaccharide is a major component of the outer leaflet of the outer membrane in Gram-negative bacteria. It is composed of three domains; lipid A, Core oligosaccharide and the O-antigen. All of these enzymes transfer heptose to the lipopolysaccharide core.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AOR38411.1	2.07e-166	6	328	2	326
AFY67702.1	2.70e-53	6	296	2	302
BAU12214.1	1.23e-51	6	293	2	301
ARV62225.1	3.25e-51	6	293	2	299
AFY60942.1	4.53e-49	5	293	4	302

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000018	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000003890_00287.