Basic Information | |
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Species | Brachypodium distachyon |
Cazyme ID | Bradi3g02700.1 |
Family | GH16 |
Protein Properties | Length: 337 Molecular Weight: 37662.6 Isoelectric Point: 8.5273 |
Chromosome | Chromosome/Scaffold: 3 Start: 1622599 End: 1624110 |
Description | xyloglucan endotransglucosylase/hydrolase 30 |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH16 | 54 | 215 | 4.9e-27 |
SLLLDRTTGSGLVSSSMYHHGFFSASIKLPSDYTAGVIVAFYTSNGEVYEKTHDELDFEFLGNIRGKPWRVQTNVYGNGSVSRGREERYVLPFDPTTEFH RYSILWTRDAIIFYVDDVPIRYIPRSSYTVDDFPSKPMSLYATIWDGSNWATSGGRYRVDYI |
Full Sequence |
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Protein Sequence Length: 337 Download |
MSPLIASAAL LLLLMLVAAA MADVTAATVA VSEGYTALFG FDNILRSADD RTVSLLLDRT 60 TGSGLVSSSM YHHGFFSASI KLPSDYTAGV IVAFYTSNGE VYEKTHDELD FEFLGNIRGK 120 PWRVQTNVYG NGSVSRGREE RYVLPFDPTT EFHRYSILWT RDAIIFYVDD VPIRYIPRSS 180 YTVDDFPSKP MSLYATIWDG SNWATSGGRY RVDYIHGPFV ASFTDLALVG CRASPAARTP 240 TDDGYCTEAE AALAASDPAV MTLAKQQAMR RFREQNMVYS YCYDTRRYPV PFPECDLVES 300 ERRRFKQSGH LRLAFRRRRR GRRPVRGVAG TAKADM* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd02183 | GH16_fungal_CRH1_transglycosylase | 9.0e-24 | 59 | 221 | 174 | + glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
cd00413 | Glyco_hydrolase_16 | 3.0e-27 | 49 | 215 | 183 | + glycosyl hydrolase family 16. The O-Glycosyl hydrolases are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A glycosyl hydrolase classification system based on sequence similarity has led to the definition of more than 95 different families inlcuding glycosyl hydrolase family 16. Family 16 includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
PLN03161 | PLN03161 | 3.0e-47 | 44 | 295 | 260 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional | ||
pfam00722 | Glyco_hydro_16 | 3.0e-66 | 33 | 216 | 184 | + Glycosyl hydrolases family 16. | ||
cd02176 | GH16_XET | 7.0e-118 | 33 | 295 | 264 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005618 | cell wall |
GO:0005975 | carbohydrate metabolic process |
GO:0006073 | cellular glucan metabolic process |
GO:0016762 | xyloglucan:xyloglucosyl transferase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ACD03224.1 | 0 | 19 | 323 | 24 | 326 | xyloglucan endotransglucosylase/hydrolase 14 [Actinidia deliciosa] |
GenBank | EAZ21594.1 | 0 | 28 | 336 | 32 | 341 | hypothetical protein OsJ_05221 [Oryza sativa Japonica Group] |
RefSeq | NP_001045764.1 | 0 | 28 | 311 | 32 | 315 | Os02g0127800 [Oryza sativa (japonica cultivar-group)] |
RefSeq | NP_001146328.1 | 0 | 28 | 323 | 32 | 326 | hypothetical protein LOC100279904 [Zea mays] |
RefSeq | XP_002451458.1 | 0 | 28 | 323 | 41 | 342 | hypothetical protein SORBIDRAFT_04g002290 [Sorghum bicolor] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2uwc_B | 0 | 28 | 295 | 16 | 271 | A Chain A, Crystal Structure Of Nasturtium Xyloglucan Hydrolase Isoform Nxg2 |
PDB | 2uwc_A | 0 | 28 | 295 | 16 | 271 | A Chain A, Crystal Structure Of Nasturtium Xyloglucan Hydrolase Isoform Nxg2 |
PDB | 2vh9_B | 0 | 1 | 295 | 1 | 290 | A Chain A, Crystal Structure Of Nxg1-Deltayniig In Complex With Xllg, A Xyloglucan Derived Oligosaccharide |
PDB | 2vh9_A | 0 | 1 | 295 | 1 | 290 | A Chain A, Crystal Structure Of Nxg1-Deltayniig In Complex With Xllg, A Xyloglucan Derived Oligosaccharide |
PDB | 2uwa_C | 0 | 28 | 295 | 19 | 274 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |