Basic Information | |
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Species | Oryza sativa |
Cazyme ID | LOC_Os09g23220.1 |
Family | GH16 |
Protein Properties | Length: 333 Molecular Weight: 36457.3 Isoelectric Point: 7.281 |
Chromosome | Chromosome/Scaffold: 9 Start: 13780703 End: 13782442 |
Description | xyloglucan endotransglucosylase/hydrolase 28 |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH16 | 57 | 228 | 1.5e-23 |
NLAVLRDGRRVRLTLDESTGAGFASQDVFLHGFFSAAVKLPAYYAAGVVVAFYLSNGDTYEKTHDEVDFEFLGNVRGREWRVQTNVYGNGSTAAGREERY DLPFDPTDELHHYSILWTRRRIIFYVDETPIREVVRTAAMGAAFPAKPMSVYATIWDGSAWATLGGRYRVNY |
Full Sequence |
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Protein Sequence Length: 333 Download |
MVVVVAMPPA LSLLVLLVLA LHGGAGDATP PPPLRLVRGA RRVAFDEGYT RMFGDGNLAV 60 LRDGRRVRLT LDESTGAGFA SQDVFLHGFF SAAVKLPAYY AAGVVVAFYL SNGDTYEKTH 120 DEVDFEFLGN VRGREWRVQT NVYGNGSTAA GREERYDLPF DPTDELHHYS ILWTRRRIIF 180 YVDETPIREV VRTAAMGAAF PAKPMSVYAT IWDGSAWATL GGRYRVNYRY APFVAEFADL 240 VLHGCAVDPL AVEHSASCGD EEEEAAEAVV SSAAMAAFRR GHMSYSYCHD RRRYPVALSE 300 CALTGGAASL GRLFGPDGMK RRRARRARDA SS* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd02175 | GH16_lichenase | 2.0e-16 | 66 | 215 | 158 | + lichenase, member of glycosyl hydrolase family 16. Lichenase, also known as 1,3-1,4-beta-glucanase, is a member of glycosyl hydrolase family 16, that specifically cleaves 1,4-beta-D-glucosidic bonds in mixed-linked beta glucans that also contain 1,3-beta-D-glucosidic linkages. Natural substrates of beta-glucanase are beta-glucans from grain endosperm cell walls or lichenan from the Islandic moss, Cetraria islandica. This protein is found not only in bacteria but also in anaerobic fungi. This domain includes two seven-stranded antiparallel beta-sheets that are adjacent to one another forming a compact, jellyroll beta-sandwich structure. | ||
cd02183 | GH16_fungal_CRH1_transglycosylase | 2.0e-18 | 119 | 235 | 132 | + glycosylphosphatidylinositol-glucanosyltransferase. Group of fungal GH16 members related to Saccharomyces cerevisiae Crh1p. Chr1p and Crh2p are transglycosylases that are required for the linkage of chitin to beta(1-3)glucose branches of beta(1-6)glucan, an important step in the assembly of new cell wall. Both have been shown to be glycosylphosphatidylinositol (GPI)-anchored. A third homologous protein, Crr1p, functions in the formation of the spore wall. They belongs to the family 16 of glycosyl hydrolases that includes lichenase, xyloglucan endotransglycosylase (XET), beta-agarase, kappa-carrageenase, endo-beta-1,3-glucanase, endo-beta-1,3-1,4-glucanase, and endo-beta-galactosidase, all of which have a conserved jelly roll fold with a deep active site channel harboring the catalytic residues. | ||
PLN03161 | PLN03161 | 2.0e-42 | 53 | 294 | 252 | + Probable xyloglucan endotransglucosylase/hydrolase protein; Provisional | ||
pfam00722 | Glyco_hydro_16 | 4.0e-56 | 45 | 230 | 186 | + Glycosyl hydrolases family 16. | ||
cd02176 | GH16_XET | 8.0e-113 | 43 | 301 | 264 | + Xyloglucan endotransglycosylase, member of glycosyl hydrolase family 16. Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Xyloglucan is a soluble hemicellulose with a backbone of beta-1,4-linked glucose units, partially substituted with alpha-1,6-linked xylopyranose branches. It binds noncovalently to cellulose, cross-linking the adjacent cellulose microfibrils, giving it a key structural role as a matrix polymer. Therefore, XET plays an important role in all plant processes that require cell wall remodeling. |
Gene Ontology | |
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GO Term | Description |
GO:0000003 | reproduction |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005576 | extracellular region |
GO:0005618 | cell wall |
GO:0005623 | cell |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | EAZ09009.1 | 0 | 23 | 332 | 23 | 332 | hypothetical protein OsI_31270 [Oryza sativa Indica Group] |
GenBank | EAZ44637.1 | 0 | 23 | 279 | 23 | 279 | hypothetical protein OsJ_29257 [Oryza sativa Japonica Group] |
RefSeq | NP_172925.1 | 0 | 13 | 331 | 7 | 331 | XTR2 (XYLOGLUCAN ENDOTRANSGLYCOSYLASE RELATED 2); hydrolase, acting on glycosyl bonds / xyloglucan:xyloglucosyl transferase [Arabidopsis thaliana] |
RefSeq | XP_002315842.1 | 0 | 8 | 316 | 8 | 309 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002462345.1 | 0 | 27 | 327 | 29 | 330 | hypothetical protein SORBIDRAFT_02g024120 [Sorghum bicolor] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2vh9_B | 0 | 7 | 301 | 1 | 290 | A Chain A, Crystal Structure Of Nxg1-Deltayniig In Complex With Xllg, A Xyloglucan Derived Oligosaccharide |
PDB | 2vh9_A | 0 | 7 | 301 | 1 | 290 | A Chain A, Crystal Structure Of Nxg1-Deltayniig In Complex With Xllg, A Xyloglucan Derived Oligosaccharide |
PDB | 2uwc_B | 0 | 43 | 301 | 17 | 271 | A Chain A, Crystal Structure Of Nasturtium Xyloglucan Hydrolase Isoform Nxg2 |
PDB | 2uwc_A | 0 | 43 | 301 | 17 | 271 | A Chain A, Crystal Structure Of Nasturtium Xyloglucan Hydrolase Isoform Nxg2 |
PDB | 2uwa_C | 0 | 43 | 301 | 20 | 274 | A Chain A, Crystal Structure Of The Nasturtium Seedling Xyloglucanase Isoform Nxg1 |