| Basic Information | |
|---|---|
| Species | Glycine max |
| Cazyme ID | Glyma13g06040.1 |
| Family | GT35 |
| Protein Properties | Length: 979 Molecular Weight: 110039 Isoelectric Point: 5.446 |
| Chromosome | Chromosome/Scaffold: 13 Start: 6339187 End: 6349183 |
| Description | Glycosyl transferase, family 35 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
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| Family | Start | End | Evalue |
| GT35 | 181 | 973 | 0 |
| ALSKLGHKLENVAYQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQRITKDGQEEVADDWLEMGNPWEIIRNDVSYPVKFYGKVVSGS DGKKHWIGGEDIKAVAHDVPIPGYKTKTTINLRLWSTKAASEEFDLSAFNAGRHTEASEALANAEKICYILYPGDESIEGKILRLKQQYTLCSASLQDII ARFERRSGTNVNWEEFPEKVAVQMNDTHPTLCIPELMRILIDVKGLSWKDAWNITQRTVAYTNHTVLPEALEKWSLDLMQKLLPRHIEIIEMIDEELIRT IIAEYGTENSDLLEKKLKEMRILENVELPAEFADIVVKSKEAIDIPSEELQSSEQAEVEERKDDEVEAVAKKNGTDESSIEDEKEELPEPVPEPPKLVRM ANLCVVGGHAVNGVAEIHSEIVKDEVFNAFYKLWPEKFQNKTNGVTPRRWIRFCNPDLSKIITEWIGTEDWVLNTGKLAELRKFVDNEDLQVQWREAKRS NKVKVAAFIREKTGYSVSPDAMFDIQVKRIHEYKRQLLNIFGIVYRYKKMKEMSAAERKANFVPRVCIFGGKAFATYVQAKRIVKFITDVGATVNHDPEI GDLLKVVFVPDYNVSVAEMLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIREEVGADNFFLFGAKAHEIAGLRKERAEGKFVPDPRF EEVKEFVRSGVFGSYNYDELMGSLEGNEGFGRADYFLVGKDFPSYIECQEKVDEAYRNQTKWTRMSILNTAGSYKFSSDRTIHEYAREIWNIE | |||
| Full Sequence |
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| Protein Sequence Length: 979 Download |
| MASSATMRFS ATSTGAEAAL PRRNSVAGFI GVAARSSAKS RLRFIGRNAN LSLRRRMSSF 60 PVVKCVSGSE AKAQDPVAKQ QEATTSLSSF SPDASSIASS IKYHAEFTPL FSPENFDLPQ 120 AFLATAQSVR DALIINWNAT YDYYEKLNVK QAYYLSMEFL QGRALLNAIG NLELTGPHAE 180 ALSKLGHKLE NVAYQEPDAA LGNGGLGRLA SCFLDSLATL NYPAWGYGLR YKYGLFKQRI 240 TKDGQEEVAD DWLEMGNPWE IIRNDVSYPV KFYGKVVSGS DGKKHWIGGE DIKAVAHDVP 300 IPGYKTKTTI NLRLWSTKAA SEEFDLSAFN AGRHTEASEA LANAEKICYI LYPGDESIEG 360 KILRLKQQYT LCSASLQDII ARFERRSGTN VNWEEFPEKV AVQMNDTHPT LCIPELMRIL 420 IDVKGLSWKD AWNITQRTVA YTNHTVLPEA LEKWSLDLMQ KLLPRHIEII EMIDEELIRT 480 IIAEYGTENS DLLEKKLKEM RILENVELPA EFADIVVKSK EAIDIPSEEL QSSEQAEVEE 540 RKDDEVEAVA KKNGTDESSI EDEKEELPEP VPEPPKLVRM ANLCVVGGHA VNGVAEIHSE 600 IVKDEVFNAF YKLWPEKFQN KTNGVTPRRW IRFCNPDLSK IITEWIGTED WVLNTGKLAE 660 LRKFVDNEDL QVQWREAKRS NKVKVAAFIR EKTGYSVSPD AMFDIQVKRI HEYKRQLLNI 720 FGIVYRYKKM KEMSAAERKA NFVPRVCIFG GKAFATYVQA KRIVKFITDV GATVNHDPEI 780 GDLLKVVFVP DYNVSVAEML IPASELSQHI STAGMEASGT SNMKFAMNGC ILIGTLDGAN 840 VEIREEVGAD NFFLFGAKAH EIAGLRKERA EGKFVPDPRF EEVKEFVRSG VFGSYNYDEL 900 MGSLEGNEGF GRADYFLVGK DFPSYIECQE KVDEAYRNQT KWTRMSILNT AGSYKFSSDR 960 TIHEYAREIW NIEPVQLP* |
| Functional Domains Download unfiltered results here | ||||||||
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| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam00343 | Phosphorylase | 2.0e-132 | 181 | 504 | 324 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 2.0e-179 | 101 | 505 | 409 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 0 | 579 | 972 | 403 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| pfam00343 | Phosphorylase | 0 | 579 | 974 | 401 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| TIGR02093 | P_ylase | 0 | 579 | 972 | 399 | + glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| Gene Ontology | |
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| GO Term | Description |
| GO:0004645 | phosphorylase activity |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
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| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | ACJ11757.1 | 0 | 58 | 978 | 2 | 935 | alpha-1,4 glucan phosphorylase [Gossypium hirsutum] |
| EMBL | CBI22291.1 | 0 | 29 | 978 | 26 | 982 | unnamed protein product [Vitis vinifera] |
| Swiss-Prot | P53536 | 0 | 3 | 977 | 2 | 1002 | PHSL_VICFA RecName: Full=Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic; AltName: Full=Starch phosphorylase L; Flags: Precursor |
| RefSeq | XP_002305367.1 | 0 | 48 | 978 | 2 | 949 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002526085.1 | 0 | 11 | 978 | 2 | 977 | glycogen phosphorylase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
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| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2azd_B | 0 | 148 | 970 | 57 | 792 | A Chain A, Recombinant Horseradish Peroxidase Phe209ser Complex With Benzhydroxamic Acid |
| PDB | 2azd_A | 0 | 148 | 970 | 57 | 792 | A Chain A, Recombinant Horseradish Peroxidase Phe209ser Complex With Benzhydroxamic Acid |
| PDB | 2aw3_B | 0 | 148 | 970 | 57 | 792 | A Chain A, Recombinant Horseradish Peroxidase Phe209ser Complex With Benzhydroxamic Acid |
| PDB | 2aw3_A | 0 | 148 | 970 | 57 | 792 | A Chain A, Recombinant Horseradish Peroxidase Phe209ser Complex With Benzhydroxamic Acid |
| PDB | 2av6_B | 0 | 148 | 970 | 57 | 792 | A Chain A, Recombinant Horseradish Peroxidase Phe209ser Complex With Benzhydroxamic Acid |
| Metabolic Pathways | |||
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| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-1826 | EC-2.4.1.1 | phosphorylase |
| Hydropathy |
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| EST Download unfiltered results here | ||||
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| Hit | Length | Start | End | EValue |
| HO778303 | 864 | 121 | 979 | 0 |
| HO797178 | 423 | 557 | 979 | 0 |
| HO613954 | 522 | 458 | 979 | 0 |
| HO620767 | 401 | 579 | 979 | 0 |
| HO778303 | 123 | 3 | 123 | 2e-37 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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