Basic Information | |
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Species | Linum usitatissimum |
Cazyme ID | Lus10023878 |
Family | GH13 |
Protein Properties | Length: 1143 Molecular Weight: 130632 Isoelectric Point: 6.24 |
Chromosome | Chromosome/Scaffold: 177 Start: 321623 End: 334279 |
Description | starch branching enzyme 2.1 |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH13 | 625 | 945 | 1.9e-29 |
LPRIKKLGYNAVQIMAIQEHSYYASFGYHVTNFFAPSSRFGTPDDLKSLIDKAHELGLVVLMDIVHSHSSNNTLDGLNMFDGTDSQYFHSGARGHHMMWD SRLFNYGSWEVVRYLLSNARWWLDEYKFDGFRFDGVTSMMYTHHGLQMTFTGNYNEYFGFATDVDAVVYLMLVNDLIHGLFPEAITIGEDVSGMPTFCIP VQDGGVGFDYRLHMAIADKWIELLKKRDEDWRMGDIVHTLTNRRWLEKCVCYAESHDQALVGDKTIAFWLMDKDMYDFMALDRPATPLIDRGIALHKMIR LITLGLGGEGYLNFMGNEFGH |
Full Sequence |
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Protein Sequence Length: 1143 Download |
MDLVELNPLR DALVGLPGVD GLSTEQRKRL TIAVELVANP SIIFMDEPTS GLDARAAAIV 60 MRTVRNTVDT GRTVVCTIHQ PSIDIFEAFD ELLLMKRGGQ VIYAGPLGRQ SHKLVEYFEA 120 VLGVPKIKDG YNPATWMLEV SSPAMEVQLD VDFAQIYANS TLYHQNQELI KELSTAEPGS 180 KDLYFPTQYS QSFTTQYRAC FWKQNWSYWR NTQYNAVRFF LTFVIGVLFG IIFWKKGQKT 240 GKQQDLMNIL GAIYAAVFFL GATNASSVQP VVSVERTVFY RERAAGMYSA LPYAFAQVSI 300 EAIYVAFQTL IYHLLLYSMI GFEWKVDKFL LFYYYIYMAF VYFTLYGMMV VALTPGHQIA 360 AICMSFFLSF WNLFAGFLIP RIALYKIPES STMVDGKNNR ILGKQGENAV LPDRRFTIGE 420 RSIPPPGAGQ RIYDIDPTLS GFRQHLDYRY SQYKRIRAEI DKFEGGLEEF SRGYEKFGFI 480 RSETGITYRE WAPGATRAAL IGDFNNWNPN ADVMTRNEFG VWEIFLPNNA DGSSPIPHGS 540 RVKIRMDTPS GLKDSIPAWI KFAVQAPGEI PYNGIYYDPP KEEKYVFKHP QPDKPKSLRI 600 YESHVGMSST EPKINTYASF RDDVLPRIKK LGYNAVQIMA IQEHSYYASF GYHVTNFFAP 660 SSRFGTPDDL KSLIDKAHEL GLVVLMDIVH SHSSNNTLDG LNMFDGTDSQ YFHSGARGHH 720 MMWDSRLFNY GSWEVVRYLL SNARWWLDEY KFDGFRFDGV TSMMYTHHGL QMTFTGNYNE 780 YFGFATDVDA VVYLMLVNDL IHGLFPEAIT IGEDVSGMPT FCIPVQDGGV GFDYRLHMAI 840 ADKWIELLKK RDEDWRMGDI VHTLTNRRWL EKCVCYAESH DQALVGDKTI AFWLMDKDMY 900 DFMALDRPAT PLIDRGIALH KMIRLITLGL GGEGYLNFMG NEFGHPEWID FPRGDQHLPN 960 GKVVPGNNNS FDKCRRRFDL GDAEYLRYHG LQEFDQAMHH LEGTYGFMTD IHQYISRKDE 1020 GDRVIVFERG NLVFVFNFHW NQSYSDYRVG CYKSGKYKIV LDSDDALFGG FNRIDHNAEF 1080 FTFDDWYDDR PRSFMVYAPA RTAVVYALVE DTTGKAPVKT ESTVALVNDN LKPLPASETT 1140 HS* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PLN03140 | PLN03140 | 1.0e-14 | 6 | 381 | 407 | + ABC transporter G family member; Provisional | ||
PLN02447 | PLN02447 | 0 | 378 | 1111 | 739 | + 1,4-alpha-glucan-branching enzyme | ||
cd11321 | AmyAc_bac_euk_BE | 0 | 582 | 996 | 416 | + Alpha amylase catalytic domain found in bacterial and eukaryotic branching enzymes. Branching enzymes (BEs) catalyze the formation of alpha-1,6 branch points in either glycogen or starch by cleavage of the alpha-1,4 glucosidic linkage yielding a non-reducing end oligosaccharide chain, and subsequent attachment to the alpha-1,6 position. By increasing the number of non-reducing ends, glycogen is more reactive to synthesis and digestion as well as being more soluble. This group includes bacterial and eukaryotic proteins. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN03140 | PLN03140 | 0 | 1 | 389 | 389 | + ABC transporter G family member; Provisional | ||
PLN02960 | PLN02960 | 0 | 536 | 1109 | 578 | + alpha-amylase |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0005975 | carbohydrate metabolic process |
GO:0016020 | membrane |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ABN05322.1 | 0 | 421 | 1111 | 136 | 826 | starch branching enzyme II [Populus trichocarpa] |
DDBJ | BAA82348.2 | 0 | 423 | 1135 | 158 | 867 | starch branching enzyme [Phaseolus vulgaris] |
EMBL | CAA56319.1 | 0 | 423 | 1124 | 159 | 859 | starch branching enzyme I [Pisum sativum] |
RefSeq | XP_002326414.1 | 0 | 421 | 1111 | 33 | 723 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002534111.1 | 0 | 389 | 1111 | 113 | 839 | starch branching enzyme II, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3amk_A | 0 | 432 | 1111 | 13 | 695 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 3vu2_B | 0 | 432 | 1111 | 13 | 695 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 3vu2_A | 0 | 432 | 1111 | 13 | 695 | A Chain A, Structure Of The Starch Branching Enzyme I (bei) Complexed With Maltopentaose From Oryza Sativa L |
PDB | 3aml_A | 0 | 432 | 1111 | 13 | 695 | A Chain A, Structure Of The Starch Branching Enzyme I (Bei) From Oryza Sativa L |
PDB | 1m7x_D | 0 | 484 | 1072 | 25 | 579 | A Chain A, The X-Ray Crystallographic Structure Of Branching Enzyme |