PlantCAZyme

Basic Information
Species	Volvox carteri
Cazyme ID	Vocar20003404m
Family	GH99
Protein Properties	Length: 409 Molecular Weight: 45485.8 Isoelectric Point: 4.8767
Chromosome	Chromosome/Scaffold: 35 Start: 318790 End: 323812
Description
View CDS

External Links
Plaza
CAZyDB

Signature Domain Download full data set without filtering

Family	Start	End	Evalue
GH99	33	365	0
VHAFYYLWYTEPSAGGWTHWDHEVLPHWDAAVAAKHTHGVRFEPPDCLHSPYYPLRGPYSSTSRELIRQHLVEMEQYGIGVLVASWWGPPWRQGSHDTQM VNTDSRLEMVIRELEESGSKVRIAFHLEPYEGRTKETVHEDLVYLSKKYRDSPAVLQINGLPVYYVYDSYRLPASDWAQLLRRNDGPLNVRGTPADGFFL GLWLDRDDGDEDILPAGFDGFYTYFSRWAVSKDLLFVPSIGPGYHGGCPGNKKQSERPSRRETLDHDTTMLLYRALWSAALSAGVQMVSITSYNEWGEGT QIEPAVPRETRLGCQYLSYEPASPYLYLEITSE

Full Sequence
Protein Sequence Length: 409 Download
MMILSAGFWL VVTALAALAL QAQGGNFPPM YRVHAFYYLW YTEPSAGGWT HWDHEVLPHW 60 DAAVAAKHTH GVRFEPPDCL HSPYYPLRGP YSSTSRELIR QHLVEMEQYG IGVLVASWWG 120 PPWRQGSHDT QMVNTDSRLE MVIRELEESG SKVRIAFHLE PYEGRTKETV HEDLVYLSKK 180 YRDSPAVLQI NGLPVYYVYD SYRLPASDWA QLLRRNDGPL NVRGTPADGF FLGLWLDRDD 240 GDEDILPAGF DGFYTYFSRW AVSKDLLFVP SIGPGYHGGC PGNKKQSERP SRRETLDHDT 300 TMLLYRALWS AALSAGVQMV SITSYNEWGE GTQIEPAVPR ETRLGCQYLS YEPASPYLYL 360 EITSEGSKRL GQLTAGGEGE TCQATSAGGN GDDGGGVGDG GSDDIAEQ*

Full Sequence

Protein Sequence Length: 409 Download

MMILSAGFWL VVTALAALAL QAQGGNFPPM YRVHAFYYLW YTEPSAGGWT HWDHEVLPHW    60
DAAVAAKHTH GVRFEPPDCL HSPYYPLRGP YSSTSRELIR QHLVEMEQYG IGVLVASWWG    120
PPWRQGSHDT QMVNTDSRLE MVIRELEESG SKVRIAFHLE PYEGRTKETV HEDLVYLSKK    180
YRDSPAVLQI NGLPVYYVYD SYRLPASDWA QLLRRNDGPL NVRGTPADGF FLGLWLDRDD    240
GDEDILPAGF DGFYTYFSRW AVSKDLLFVP SIGPGYHGGC PGNKKQSERP SRRETLDHDT    300
TMLLYRALWS AALSAGVQMV SITSYNEWGE GTQIEPAVPR ETRLGCQYLS YEPASPYLYL    360
EITSEGSKRL GQLTAGGEGE TCQATSAGGN GDDGGGVGDG GSDDIAEQ* 

Functional Domains Download unfiltered results here

Cdd ID	Domain	E-Value	Start	End	Length	Domain Description
cd11576	GH99_GH71_like_2	3.0e-6	33	370	395	+ Uncharacterized glycoside hydrolase family 99-like domain. This family of putative glycoside hydrolases resembles glycosyl hydrolase families 71 and 99 (following the CAZY nomenclature) and may share a similar catalytic site and mechanism. The domain may co-occur with other domains involved in the binding/processing of glycans.
cd11578	GH99_GH71_like_1	4.0e-14	79	339	294	+ Uncharacterized glycoside hydrolase family 99-like domain. This family of putative glycoside hydrolases resembles glycosyl hydrolase families 71 and 99 (following the CAZY nomenclature) and may share a similar catalytic site and mechanism.
cd11575	GH99_GH71_like_3	3.0e-16	33	363	370	+ Uncharacterized glycoside hydrolase family 99-like domain. This family of putative glycoside hydrolases resembles glycosyl hydrolase families 71 and 99 (following the CAZY nomenclature) and may share a similar catalytic site and mechanism.
cd11573	GH99_GH71_like	1.0e-30	97	363	292	+ Glycoside hydrolase families 71, 99, and related domains. This superfamily of glycoside hydrolases contains families GH71 and GH99 (following the CAZY nomenclature), as well as other members with undefined function and specificity.
cd11574	GH99	1.0e-112	33	365	356	+ Glycoside hydrolase family 99, an endo-alpha-1,2-mannosidase. This family of glycoside hydrolases 99 (following the CAZY nomenclature) includes endo-alpha-1,2-mannosidase (EC 3.2.1.130), which is an important membrane-associated eukaryotic enzyme involved in the maturation of N-linked glycans. Specifically, it cleaves mannoside linkages internal to N-linked glycan chains by hydrolyzing an alpha-1,2-mannosidic bond between a glucose-substituted mannose and the remainder of the chain. The biological function and significance of the soluble bacterial orthologs, which may have obtained the genes via horizontal transfer, is not clear.

Annotations - NR Download unfiltered results here
Source	Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
RefSeq	XP_001056614.1	0	33	363	100	436	PREDICTED: similar to mannosidase, endo-alpha [Rattus norvegicus]
RefSeq	XP_001170001.1	0	33	363	105	441	PREDICTED: similar to mannosidase, endo-alpha-like isoform 3 [Pan troglodytes]
RefSeq	XP_001633129.1	0	29	372	6	355	predicted protein [Nematostella vectensis]
RefSeq	XP_001691860.1	0	41	370	32	371	hypothetical protein CHLREDRAFT_145451 [Chlamydomonas reinhardtii]
RefSeq	XP_417758.2	0	29	372	60	409	PREDICTED: hypothetical protein [Gallus gallus]

Annotations - PDB Download unfiltered results here
Source	Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
PDB	4ad0_D	0	35	360	44	361	A Chain A, Wif Domain-Epidermal Growth Factor (Egf)-Like Domains 1-3 Of Human Wnt Inhibitory Factor 1 In Complex With 1,2-Dipalmitoylphosphatidylcholine
PDB	4ad0_C	0	35	360	44	361	A Chain A, Wif Domain-Epidermal Growth Factor (Egf)-Like Domains 1-3 Of Human Wnt Inhibitory Factor 1 In Complex With 1,2-Dipalmitoylphosphatidylcholine
PDB	4ad0_B	0	35	360	44	361	A Chain A, Wif Domain-Epidermal Growth Factor (Egf)-Like Domains 1-3 Of Human Wnt Inhibitory Factor 1 In Complex With 1,2-Dipalmitoylphosphatidylcholine
PDB	4ad0_A	0	35	360	44	361	A Chain A, Wif Domain-Epidermal Growth Factor (Egf)-Like Domains 1-3 Of Human Wnt Inhibitory Factor 1 In Complex With 1,2-Dipalmitoylphosphatidylcholine
PDB	4acz_A	0	35	360	44	361	A Chain A, Structure Of The Gh99 Endo-Alpha-Mannosidase From Bacteroides Thetaiotaomicron

EST Download unfiltered results here

Hit	Length	Start	End	EValue
HO627855	386	32	383	0
EH669148	237	33	259	4.99983e-42
GW595129	207	176	365	6e-40
HO639020	145	32	174	8e-40
HO434704	191	192	365	3e-32

Orthologous Group
Species	ID
Chlamydomonas reinhardtii	Cre03.g189050.t1.3

Signal Peptide

Cleavage Site
24

CAZyme Information