Basic Information | |
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Species | Eucalyptus grandis |
Cazyme ID | Eucgr.A02888.1 |
Family | GH32 |
Protein Properties | Length: 655 Molecular Weight: 72386.4 Isoelectric Point: 4.301 |
Chromosome | Chromosome/Scaffold: 1 Start: 39269769 End: 39275116 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 122 | 440 | 0 |
HFQPEKNWMNDPDGPLYYMGWYHLFYQYNPDSAVWGNITWGHAVSKDLIHWLYLPLAMVPDRWFDIGGVWTGSATLLPDGRIVMLYTGDMANSVQVQNLA YPANLSDPLLLDWVKYPDNPVIVPPAGIEYDEFRDPTTAWIGPNGTWRLLIGTKVNSTGMALIYETTNFTTYEAAPGVLHAVNGTGMWECVDFYPVSTNS SEGLETSVVGPGVKHVLKASLDDLKMDYYAIGTYDPVSDTWTPDDPEADVGIGLLVDYGRYYASKTFFDQNTGRRILWGWINETDTDYDDLAKGWASVQT IPRVVTFDNKTGSNILEWP |
Full Sequence |
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Protein Sequence Length: 655 Download |
MENSPSNTNA PYTLLPEDPF PARPLASFRR PLKVFGATVA SVVFLMSLVG LIINQSPVPP 60 AKNDHHGSDP LTSKATSFSE DAERLPTGVS EGVSAKSYPS VRDRLSYNWT NAMFSWQRTA 120 YHFQPEKNWM NDPDGPLYYM GWYHLFYQYN PDSAVWGNIT WGHAVSKDLI HWLYLPLAMV 180 PDRWFDIGGV WTGSATLLPD GRIVMLYTGD MANSVQVQNL AYPANLSDPL LLDWVKYPDN 240 PVIVPPAGIE YDEFRDPTTA WIGPNGTWRL LIGTKVNSTG MALIYETTNF TTYEAAPGVL 300 HAVNGTGMWE CVDFYPVSTN SSEGLETSVV GPGVKHVLKA SLDDLKMDYY AIGTYDPVSD 360 TWTPDDPEAD VGIGLLVDYG RYYASKTFFD QNTGRRILWG WINETDTDYD DLAKGWASVQ 420 TIPRVVTFDN KTGSNILEWP VEEVESLRLD STEFDDILVE AGSVVALNIS SATQLDISVE 480 FEIEGSDALE ALEVPEAIIE CATTQNRSTL GPFGLLVLAD ESLSELTPIY FHIAKSTNGS 540 LTTLFCADET RSSKASEIFK KVYGGPVPVL NDEKFTMRLL VDHSIIESFA QGGRTVITSR 600 VYPTEAIYGA AKLFLFNNAT GVNVTASLEI WQMNSAFIHP FPLEELGGGR FSQK* 660 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 5.0e-55 | 118 | 603 | 503 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 4.0e-73 | 118 | 632 | 531 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 4.0e-93 | 128 | 443 | 328 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 5.0e-145 | 122 | 440 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 0 | 122 | 593 | 482 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ACQ82802.1 | 0 | 1 | 645 | 1 | 635 | vacuolar invertase [Gossypium hirsutum] |
DDBJ | BAF35858.1 | 0 | 6 | 646 | 3 | 645 | Soluble acid invertase [Pyrus pyrifolia var. culta] |
DDBJ | BAG30919.1 | 0 | 6 | 646 | 3 | 645 | soluble acid invertase [Pyrus pyrifolia] |
RefSeq | XP_002265534.1 | 0 | 11 | 645 | 21 | 648 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002510944.1 | 0 | 1 | 645 | 1 | 638 | Beta-fructofuranosidase, soluble isoenzyme I precursor, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 107 | 641 | 8 | 544 | B Chain B, Reassembly And Co-Crystallization Of A Family 9 Processive Endoglucanase From Separately Expressed Gh9 And Cbm3c Modules |
PDB | 3ugh_A | 0 | 107 | 641 | 8 | 544 | B Chain B, Reassembly And Co-Crystallization Of A Family 9 Processive Endoglucanase From Separately Expressed Gh9 And Cbm3c Modules |
PDB | 3ugg_B | 0 | 107 | 641 | 8 | 544 | B Chain B, Reassembly And Co-Crystallization Of A Family 9 Processive Endoglucanase From Separately Expressed Gh9 And Cbm3c Modules |
PDB | 3ugg_A | 0 | 107 | 641 | 8 | 544 | B Chain B, Reassembly And Co-Crystallization Of A Family 9 Processive Endoglucanase From Separately Expressed Gh9 And Cbm3c Modules |
PDB | 3ugf_B | 0 | 107 | 641 | 8 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |