Basic Information | |
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Species | Physcomitrella patens |
Cazyme ID | Pp1s405_3V6.1 |
Family | GH32 |
Protein Properties | Length: 518 Molecular Weight: 58001 Isoelectric Point: 4.6493 |
Chromosome | Chromosome/Scaffold: 405 Start: 31883 End: 34970 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 1 | 310 | 0 |
MYYEGFYHLFYQYNPGGAVWGNLTWGHAVSTDLIHWRDLEPALKPDEWYDNGGVWSGSVTICPDGSPLILYTGTIAPSGVADDLEQSQNLAVPEDLADPL LRKWVKSRENPILRHPVGIDKEDFRDPTTAWQVNDGTWRILVGAKMGRDGMALLYKSEDLRHWELDENVLHTVPGSGMWECLDFFPIAPFGREGLDTSVN GPHVKHVLKASMYDDQHDHYAVGTYNLSTESFTPINHALDIQHGLHYDYGKFYASKSFYDPVKKRRIVWGWSNESDSAAQDIARGWASLQAIPRVLWLDT ALGDSLIQAP |
Full Sequence |
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Protein Sequence Length: 518 Download |
MYYEGFYHLF YQYNPGGAVW GNLTWGHAVS TDLIHWRDLE PALKPDEWYD NGGVWSGSVT 60 ICPDGSPLIL YTGTIAPSGV ADDLEQSQNL AVPEDLADPL LRKWVKSREN PILRHPVGID 120 KEDFRDPTTA WQVNDGTWRI LVGAKMGRDG MALLYKSEDL RHWELDENVL HTVPGSGMWE 180 CLDFFPIAPF GREGLDTSVN GPHVKHVLKA SMYDDQHDHY AVGTYNLSTE SFTPINHALD 240 IQHGLHYDYG KFYASKSFYD PVKKRRIVWG WSNESDSAAQ DIARGWASLQ AIPRVLWLDT 300 ALGDSLIQAP IEEVDDLRVG KVSKTDVDLE AGSVIKIEGS SGGQLDIEVI FEYPNVSNVI 360 VQDYGFLNGP FDCGNGGSAQ RGVYGPFGLL VLTDDAYQEQ TAVFFYIAQD ANQRWVTHFC 420 SDQSRSSLLH DIDTTAFWSD VRVLPTENFL SLRVLVDHSI VESFVQGGRM AITSRVYPKE 480 AVDEKAHVFL FNNSTTQITV RSINVWQMRS ITVLPLA* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 2.0e-53 | 2 | 479 | 489 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 8.0e-64 | 1 | 480 | 486 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 4.0e-89 | 1 | 313 | 316 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 4.0e-117 | 1 | 310 | 318 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 3.0e-153 | 1 | 468 | 476 | + Glycosyl hydrolases family 32. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CAA53098.1 | 0 | 1 | 516 | 150 | 656 | beta-fructofuranosidase [Daucus carota] |
EMBL | CAA53099.1 | 0 | 1 | 516 | 150 | 656 | beta-fructofuranosidase [Daucus carota] |
RefSeq | XP_001764172.1 | 0 | 1 | 513 | 54 | 561 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_001783601.1 | 0 | 1 | 517 | 28 | 542 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_001784437.1 | 0 | 1 | 517 | 1 | 517 | predicted protein [Physcomitrella patens subsp. patens] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 1 | 516 | 38 | 544 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
PDB | 3ugh_A | 0 | 1 | 516 | 38 | 544 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
PDB | 3ugg_B | 0 | 1 | 516 | 38 | 544 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
PDB | 3ugg_A | 0 | 1 | 516 | 38 | 544 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
PDB | 3ugf_B | 0 | 1 | 516 | 38 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
FC369012 | 242 | 277 | 518 | 0 |
FC369013 | 238 | 1 | 238 | 0 |
CT842376 | 514 | 1 | 509 | 0 |
BJ173325 | 175 | 344 | 518 | 0 |
DY265807 | 311 | 1 | 311 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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