Basic Information | |
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Species | Populus trichocarpa |
Cazyme ID | Potri.015G127100.1 |
Family | GH32 |
Protein Properties | Length: 640 Molecular Weight: 71765.4 Isoelectric Point: 4.7795 |
Chromosome | Chromosome/Scaffold: 15 Start: 13942984 End: 13947656 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 118 | 437 | 0 |
HFQPEKNWMNDPDGPLFHKGWYHLFYQYNPDSAVWGNITWGHAVSTDLIHWLYLPFAMVPDHWYDINGVWTGSATLLPDGQIMMLYTGSTNESVQVQNLA YPANLSDPLLIDWVKYPNNPVITPPNGTETDEFRDPTTAWMGPDGTWRITIGSRHNKSIGISLVYQTSNFTTYELLEGVLHAVPGTGMWECVDFYPVAIN GSTGLDTSAYGAGIKHVLKASLDDTKRDHYAIGVYDPVTDKWTPDNPKEDVGIGLQVDYGRYYASKTFYDQNTQRRILWGWINETDTETDDLDKGWASVQ TIPRKVLYDNKTGTNILQWP |
Full Sequence |
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Protein Sequence Length: 640 Download |
MDTNPSHTSS DPPYTPLLDN PSPARIRRPF NGFAAILASL IFLLSLVALI INQSQESLPE 60 QNQNRSPSTP RPTESFSKPE PRGVAQGVSP KSNPSFFSDK VSYNWTNAMF SWQRTAYHFQ 120 PEKNWMNDPD GPLFHKGWYH LFYQYNPDSA VWGNITWGHA VSTDLIHWLY LPFAMVPDHW 180 YDINGVWTGS ATLLPDGQIM MLYTGSTNES VQVQNLAYPA NLSDPLLIDW VKYPNNPVIT 240 PPNGTETDEF RDPTTAWMGP DGTWRITIGS RHNKSIGISL VYQTSNFTTY ELLEGVLHAV 300 PGTGMWECVD FYPVAINGST GLDTSAYGAG IKHVLKASLD DTKRDHYAIG VYDPVTDKWT 360 PDNPKEDVGI GLQVDYGRYY ASKTFYDQNT QRRILWGWIN ETDTETDDLD KGWASVQTIP 420 RKVLYDNKTG TNILQWPVEE IESLRLRSTD FTEIVVGPGS VVPLDIGQAT QLDIFAEFEV 480 EIISETKHEK YGCSGGAVDR SALGPFGLLV VADQTLSELT PIFFRPVNTT EGIVETYFCA 540 DETRSSKASD VYKQVYGSTV PVFTDEKFQM RVLVDHSIVE SFAQGGRRVI TSRIYPTKAI 600 YGDARLFLFN NATGVNVKAT LKIWELNSAF IHPFLFDQN* |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 6.0e-52 | 114 | 596 | 502 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 1.0e-75 | 93 | 628 | 548 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 2.0e-90 | 124 | 440 | 328 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 4.0e-147 | 118 | 437 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 0 | 118 | 586 | 478 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ACJ04702.1 | 0 | 8 | 635 | 4 | 630 | invertase 2 [Cucumis melo] |
GenBank | ACQ82802.1 | 0 | 6 | 638 | 5 | 635 | vacuolar invertase [Gossypium hirsutum] |
DDBJ | BAF34363.1 | 0 | 19 | 638 | 25 | 641 | beta-fructofuranosidase [Citrus sinensis] |
RefSeq | XP_002321831.1 | 0 | 109 | 639 | 1 | 528 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002510944.1 | 0 | 1 | 639 | 1 | 639 | Beta-fructofuranosidase, soluble isoenzyme I precursor, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 96 | 634 | 1 | 544 | A Chain A, Hevamine Mutant D125aE127A IN COMPLEX WITH TETRA-Nag |
PDB | 3ugh_A | 0 | 96 | 634 | 1 | 544 | A Chain A, Hevamine Mutant D125aE127A IN COMPLEX WITH TETRA-Nag |
PDB | 3ugg_B | 0 | 96 | 634 | 1 | 544 | A Chain A, Hevamine Mutant D125aE127A IN COMPLEX WITH TETRA-Nag |
PDB | 3ugg_A | 0 | 96 | 634 | 1 | 544 | A Chain A, Hevamine Mutant D125aE127A IN COMPLEX WITH TETRA-Nag |
PDB | 3ugf_B | 0 | 96 | 634 | 1 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |
Metabolic Pathways | |||
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Pathway Name | Reaction | EC | Protein Name |
fructan biosynthesis | RXN-1781 | EC-2.4.1.99 | sucrose:sucrose fructosyltransferase |
sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |