| Basic Information | |
|---|---|
| Species | Vitis vinifera |
| Cazyme ID | GSVIVT01011504001 |
| Family | GT35 |
| Protein Properties | Length: 983 Molecular Weight: 111386 Isoelectric Point: 4.9472 |
| Chromosome | Chromosome/Scaffold: 14 Start: 30082284 End: 30107432 |
| Description | Glycosyl transferase, family 35 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
 | |||
| Family | Start | End | Evalue |
| GT35 | 177 | 501 | 0 |
| ALRELGKDLENVARQEPDAALGNGGLGRLASCFLDSLATLNYPAWGYGLRYKYGLFKQHITKDGQEEVAEDWLEMGNPWEIVRNDVSYPVKFYGKVIEGS DGKRHWIGGEDIIAIAYDVPIPGYKTKTTINLRLWSTKVQSDDFDLYDFNAGNHTKACEAQLNAEKICYILYPGDDSMEGKVLRLKQQYTLCSASLQDII ARFERRSGGYVNWEEFPEKVAVQMNDTHPTLCIPELMRILMDLKGMSWKEAWKITQRTVAYTNHTVLPEALEKWSLELMQKLLPRHVEIIEMIDEELINT IISEYGTADPVLLEKKLKAMRILEN | |||
| GT35 | 577 | 977 | 0 |
| EPPKMVRMANLCVVGGHAVNGVAEIHSEIVKDEVFNDFFKLWPEKFQNKTNGVTPRRWIRFCNPDLSEIITKWIHTEDWVLNTEKLSELRKFADDEELHA EWRAAKRSNKMKVVSFLKEKTGYLVSPDAMFDVQVKRIHEYKRQLLNILGIVYRYKKMKEMTAAERKAKFVPRVCIFGGKAFATYVQAKRIVKFITDVGT TVNHDSEIGDLLKVVFVPDYNVSVAELLIPASELSQHISTAGMEASGTSNMKFAMNGCILIGTLDGANVEIRQEVGEDNFFLFGAQAHEIAGLRKERAEG KFVPDPRFEEVKEFVRSGIFGPCNYDELIGSLEGNEGFGQADYFLVGKDFPSYIECQEKVDEAYGDQKRWTRMSILNAAGSYKFSSDRTIHEYAKDIWNI E | |||
| Full Sequence |
|---|
| Protein Sequence Length: 983 Download |
| MASSSFSTAS TAPHAYSHCY SISRSFVGGF SSRPSHSKLF FLRNTSASRF ATRAFPVRSV 60 FSEPHRKLKD EDPITPHGPS GTPVSLTADA ACIVSSIKYH AEFTPLFSPE QFELPKAFFA 120 TAQSVRDALI INWNATYDYH EKMNVKQAYY LSMEFLQGRA LLNAIGNLEL TGAYAEALRE 180 LGKDLENVAR QEPDAALGNG GLGRLASCFL DSLATLNYPA WGYGLRYKYG LFKQHITKDG 240 QEEVAEDWLE MGNPWEIVRN DVSYPVKFYG KVIEGSDGKR HWIGGEDIIA IAYDVPIPGY 300 KTKTTINLRL WSTKVQSDDF DLYDFNAGNH TKACEAQLNA EKICYILYPG DDSMEGKVLR 360 LKQQYTLCSA SLQDIIARFE RRSGGYVNWE EFPEKVAVQM NDTHPTLCIP ELMRILMDLK 420 GMSWKEAWKI TQRTVAYTNH TVLPEALEKW SLELMQKLLP RHVEIIEMID EELINTIISE 480 YGTADPVLLE KKLKAMRILE NVDFPASVKD LLVQPEESSV VEPGEEIQSF DEEVELIDEE 540 EELIELIDEE EEFIDEEEEP TGKGTQKKKV LSEPVPEPPK MVRMANLCVV GGHAVNGVAE 600 IHSEIVKDEV FNDFFKLWPE KFQNKTNGVT PRRWIRFCNP DLSEIITKWI HTEDWVLNTE 660 KLSELRKFAD DEELHAEWRA AKRSNKMKVV SFLKEKTGYL VSPDAMFDVQ VKRIHEYKRQ 720 LLNILGIVYR YKKMKEMTAA ERKAKFVPRV CIFGGKAFAT YVQAKRIVKF ITDVGTTVNH 780 DSEIGDLLKV VFVPDYNVSV AELLIPASEL SQHISTAGME ASGTSNMKFA MNGCILIGTL 840 DGANVEIRQE VGEDNFFLFG AQAHEIAGLR KERAEGKFVP DPRFEEVKEF VRSGIFGPCN 900 YDELIGSLEG NEGFGQADYF LVGKDFPSYI ECQEKVDEAY GDQKRWTRMS ILNAAGSYKF 960 SSDRTIHEYA KDIWNIEPVE LP* |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
 | ||||||||
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| pfam00343 | Phosphorylase | 2.0e-139 | 177 | 500 | 324 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 0 | 577 | 976 | 405 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| cd04300 | GT1_Glycogen_Phosphorylase | 0 | 96 | 501 | 410 | + This is a family of oligosaccharide phosphorylases. It includes yeast and mammalian glycogen phosphorylases, plant starch/glucan phosphorylase, as well as the maltodextrin phosphorylases of bacteria. The members of this family catalyze the breakdown of oligosaccharides into glucose-1-phosphate units. They are important allosteric enzymes in carbohydrate metabolism. The allosteric control mechanisms of yeast and mammalian members of this family are different from that of bacterial members. The members of this family belong to the GT-B structural superfamily of glycoslytransferases, which have characteristic N- and C-terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. | ||
| pfam00343 | Phosphorylase | 0 | 580 | 978 | 404 | + Carbohydrate phosphorylase. The members of this family catalyze the formation of glucose 1-phosphate from one of the following polyglucoses; glycogen, starch, glucan or maltodextrin. | ||
| TIGR02093 | P_ylase | 0 | 580 | 976 | 402 | + glycogen/starch/alpha-glucan phosphorylases. This family consists of phosphorylases. Members use phosphate to break alpha 1,4 linkages between pairs of glucose residues at the end of long glucose polymers, releasing alpha-D-glucose 1-phosphate. The nomenclature convention is to preface the name according to the natural substrate, as in glycogen phosphorylase, starch phosphorylase, maltodextrin phosphorylase, etc. Name differences among these substrates reflect differences in patterns of branching with alpha 1,6 linkages. Members include allosterically regulated and unregulated forms. A related family, TIGR02094, contains examples known to act well on particularly small alpha 1,4 glucans, as may be found after import from exogenous sources [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| Gene Ontology | |
|---|---|
| GO Term | Description |
| GO:0004645 | phosphorylase activity |
| GO:0005975 | carbohydrate metabolic process |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| EMBL | CBI22291.1 | 0 | 1 | 982 | 1 | 982 | unnamed protein product [Vitis vinifera] |
| Swiss-Prot | P27598 | 0 | 89 | 982 | 68 | 955 | PHSL_IPOBA RecName: Full=Alpha-1,4 glucan phosphorylase L isozyme, chloroplastic/amyloplastic; AltName: Full=Starch phosphorylase L; Flags: Precursor |
| RefSeq | XP_002279075.1 | 0 | 13 | 982 | 13 | 958 | PREDICTED: similar to Alpha-1,4 glucan phosphorylase L-1 isozyme, chloroplastic/amyloplastic [Vitis vinifera] |
| RefSeq | XP_002305367.1 | 0 | 62 | 982 | 22 | 949 | predicted protein [Populus trichocarpa] |
| RefSeq | XP_002526085.1 | 0 | 1 | 982 | 1 | 977 | glycogen phosphorylase, putative [Ricinus communis] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1z8d_A | 0 | 580 | 978 | 438 | 830 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| PDB | 1z8d_A | 0 | 100 | 500 | 35 | 433 | A Chain A, Crystal Structure Of Human Muscle Glycogen Phosphorylase A With Amp And Glucose |
| PDB | 1xoi_B | 0 | 577 | 981 | 434 | 832 | A Chain A, Human Liver Glycogen Phosphorylase A Complexed With Chloroindoloyl Glycine Amide |
| PDB | 1xoi_B | 0 | 89 | 481 | 23 | 418 | A Chain A, Human Liver Glycogen Phosphorylase A Complexed With Chloroindoloyl Glycine Amide |
| PDB | 1xoi_A | 0 | 577 | 981 | 434 | 832 | A Chain A, Human Liver Glycogen Phosphorylase A Complexed With Chloroindoloyl Glycine Amide |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| starch degradation I | RXN-1826 | EC-2.4.1.1 | phosphorylase |
| Hydropathy |
|---|
 |
| EST Download unfiltered results here | ||||
|---|---|---|---|---|
 | ||||
| Hit | Length | Start | End | EValue |
| HO797178 | 401 | 583 | 983 | 0 |
| HO778303 | 401 | 583 | 983 | 0 |
| HO778303 | 433 | 84 | 514 | 0 |
| HO613954 | 403 | 581 | 983 | 0 |
| HO778303 | 50 | 71 | 119 | 0.0000007 |
| Sequence Alignments (This image is cropped. Click for full image.) |
|---|
 |
