| Basic Information | |
|---|---|
| Species | Selaginella moellendorffii |
| Cazyme ID | 127793 |
| Family | GH32 |
| Protein Properties | Length: 542 Molecular Weight: 60276.7 Isoelectric Point: 8.7162 |
| View CDS | |
| External Links |
|---|
| NCBI Taxonomy |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GH32 | 24 | 339 | 0 |
| HFQPIKNWMNGPMLYKGLYHLFFQYNPTAPVFGNISWGHAVSKDLINWSFLDLALQRDKPYDQNGAFSGSITFVKGVPVILYTGSALNLDQSQNEAVPAN ISDPLVRTWKKLERNPIIFPPPSGVRTVDFRDPTTAWIGADGLWRILVGAKKNATGAAILYTSKDFVHWDLVDNPLHEVAGTGMWECPDFYPVSSFGTKG LEDSVRGSGVKHVLKVSLDNTRQDAYAVGTYDAAADKFIPNVPELDTGIGLVYDYGVFYASKTFYDPEKQRRVLWGWVTEKDSVEADIAKGWAGVQALPR QIWLDETHQNGVRQWP | |||
| Full Sequence |
|---|
| Protein Sequence Length: 542 Download |
| MLLIIIILSL AGSSVGDDLR PAFHFQPIKN WMNGPMLYKG LYHLFFQYNP TAPVFGNISW 60 GHAVSKDLIN WSFLDLALQR DKPYDQNGAF SGSITFVKGV PVILYTGSAL NLDQSQNEAV 120 PANISDPLVR TWKKLERNPI IFPPPSGVRT VDFRDPTTAW IGADGLWRIL VGAKKNATGA 180 AILYTSKDFV HWDLVDNPLH EVAGTGMWEC PDFYPVSSFG TKGLEDSVRG SGVKHVLKVS 240 LDNTRQDAYA VGTYDAAADK FIPNVPELDT GIGLVYDYGV FYASKTFYDP EKQRRVLWGW 300 VTEKDSVEAD IAKGWAGVQA LPRQIWLDET HQNGVRQWPL AEVYKLRRRD YHSQAYRKLQ 360 GGALQEIHSG RKKFQLDIEV TFTVRNHKLV TLDSIMEDGQ TSCLGRTTAS REAIGPFGLM 420 VLASDYLQEH TAIYFALLRS KSGWKPLACS DIRRSSLQPN VTKTAYGGFV NVTSTEQQLT 480 MRVIVDHSII ETFFQGGRTC ITSRVYPTSG SPRLFLFNNG SAPIGASIAL WGMKNATFTL 540 S* 600 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| TIGR01322 | scrB_fam | 5.0e-52 | 17 | 507 | 515 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
| COG1621 | SacC | 2.0e-62 | 17 | 511 | 512 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
| cd08996 | GH32_B_Fructosidase | 6.0e-85 | 30 | 342 | 329 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
| pfam00251 | Glyco_hydro_32N | 5.0e-132 | 24 | 339 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
| smart00640 | Glyco_32 | 9.0e-155 | 24 | 497 | 486 | + Glycosyl hydrolases family 32. | ||
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 2ac1_A | 0 | 14 | 539 | 3 | 541 | A Chain A, Crystal Structure Of A Cell-Wall Invertase From Arabidopsis Thaliana |
| PDB | 2xqr_K | 0 | 20 | 539 | 5 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_I | 0 | 20 | 539 | 5 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_G | 0 | 20 | 539 | 5 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| PDB | 2xqr_E | 0 | 20 | 539 | 5 | 537 | B Chain B, Crystal Structure Of Plant Cell Wall Invertase In Complex With A Specific Protein Inhibitor |
| Metabolic Pathways | |||
|---|---|---|---|
| Pathway Name | Reaction | EC | Protein Name |
| sucrose degradation III | RXN-1461 | EC-3.2.1.26 | β-fructofuranosidase |
