Basic Information | |
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Species | Ricinus communis |
Cazyme ID | 29630.m000833 |
Family | GH13 |
Protein Properties | Length: 972 Molecular Weight: 110239 Isoelectric Point: 6.1123 |
Chromosome | Chromosome/Scaffold: 29630 Start: 360643 End: 369947 |
Description | alpha-amylase-like 3 |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH13 | 602 | 887 | 6.1e-39 |
ADLSQCGVTAVWLPPPTESVAPQGYMPSDLYNLNSAYGTEEELKYCIEEMHSHDILALGDVVLNHRCAQKQSPNGVWNIFGGKLAWGPEAIVCDDPNFQG CGNPSSGDIFHAAPNIDHSQDFVRRDIKEWLNWLRNHIGFDGWRLDFVRGFSGTYVKEYIETSNPAFAIGEYWDSLAYEHGSLCYNQDAHRQRIINWINA TGGTSSAFDVTTKGILHSALHNQYWRLIDPQGKPTGVMGWWPSRAVTFLENHDTGSTQGHWPFPRDKLAQGYAYILTHPGTPVIFY |
Full Sequence |
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Protein Sequence Length: 972 Download |
MGGILLPGAV SGIPPSYHYF CSASLDKNVP HSSILHHPLI FPSSYTWKRR LFYNGSWHCK 60 SRTVVLSSME ESNDTFTGVV NSGDDLSSQR AQVLEDEENE LVAAKKALSD VRSKQETLEK 120 ERDLLLEELA RSEAKQKEYV ATILQDKELA ISELEAAKSL FHQKLQKTVE EKFALESRLV 180 LAKQDAVELA VQVEKLTEIA FQQATSHILE DAQMRVAAAE TTAAEAAFQI EEQIRNATEG 240 TIFTIVQQSK DAIDKALDVA EKAGDHAAKA VAIFSDGANP FDEIASIKSE NIRLEGVVND 300 LESHLLITRS EIDKLKAELD QVRSQAKASE VRANNAEKTL LEFQKSNREK AMQQEEEISS 360 LLEKMRKDAS ERKKAASKAF KSEVESIKAA IEAAKETARS RENAYMRRCE SLQRSLRASE 420 SALKMWRQRA EMAESLILDA EKDEDSISIV NGGRIDLLTD DDSQKWKLLS DGPRREIPQW 480 MARRIRTIRP KFPPRKTNIS EALTKNFRHL DLPKPDEVWS IAQEKPKVGD TLIEHVMEKE 540 TIEKKRKALE RVLQRKTIQW QRTPEHTKLE PGTGTGREIV FQGFNWESWR RQWYVELATK 600 MADLSQCGVT AVWLPPPTES VAPQGYMPSD LYNLNSAYGT EEELKYCIEE MHSHDILALG 660 DVVLNHRCAQ KQSPNGVWNI FGGKLAWGPE AIVCDDPNFQ GCGNPSSGDI FHAAPNIDHS 720 QDFVRRDIKE WLNWLRNHIG FDGWRLDFVR GFSGTYVKEY IETSNPAFAI GEYWDSLAYE 780 HGSLCYNQDA HRQRIINWIN ATGGTSSAFD VTTKGILHSA LHNQYWRLID PQGKPTGVMG 840 WWPSRAVTFL ENHDTGSTQG HWPFPRDKLA QGYAYILTHP GTPVIFYDHF YDFGVRDIIT 900 ELVEARKRAG IHCRSSVKIY HANNEGYVAQ IGDTLVMKLG DFDWNPSKEN NLDGSWQKFV 960 DKGADYQLWL RQ |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
PRK09441 | PRK09441 | 4.0e-38 | 578 | 908 | 419 | + cytoplasmic alpha-amylase; Reviewed | ||
PLN00196 | PLN00196 | 5.0e-146 | 578 | 940 | 376 | + alpha-amylase; Provisional | ||
cd11314 | AmyAc_arch_bac_plant_AmyA | 5.0e-167 | 579 | 917 | 343 | + Alpha amylase catalytic domain found in archaeal, bacterial, and plant Alpha-amylases (also called 1,4-alpha-D-glucan-4-glucanohydrolase). AmyA (EC 3.2.1.1) catalyzes the hydrolysis of alpha-(1,4) glycosidic linkages of glycogen, starch, related polysaccharides, and some oligosaccharides. This group includes AmyA from bacteria, archaea, water fleas, and plants. The Alpha-amylase family comprises the largest family of glycoside hydrolases (GH), with the majority of enzymes acting on starch, glycogen, and related oligo- and polysaccharides. These proteins catalyze the transformation of alpha-1,4 and alpha-1,6 glucosidic linkages with retention of the anomeric center. The protein is described as having 3 domains: A, B, C. A is a (beta/alpha) 8-barrel; B is a loop between the beta 3 strand and alpha 3 helix of A; C is the C-terminal extension characterized by a Greek key. The majority of the enzymes have an active site cleft found between domains A and B where a triad of catalytic residues (Asp, Glu and Asp) performs catalysis. Other members of this family have lost the catalytic activity as in the case of the human 4F2hc, or only have 2 residues that serve as the catalytic nucleophile and the acid/base, such as Thermus A4 beta-galactosidase with 2 Glu residues (GH42) and human alpha-galactosidase with 2 Asp residues (GH31). The family members are quite extensive and include: alpha amylase, maltosyltransferase, cyclodextrin glycotransferase, maltogenic amylase, neopullulanase, isoamylase, 1,4-alpha-D-glucan maltotetrahydrolase, 4-alpha-glucotransferase, oligo-1,6-glucosidase, amylosucrase, sucrose phosphorylase, and amylomaltase. | ||
PLN02361 | PLN02361 | 8.0e-179 | 576 | 969 | 399 | + alpha-amylase | ||
PLN02784 | PLN02784 | 0 | 568 | 969 | 404 | + alpha-amylase |
Gene Ontology | |
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GO Term | Description |
GO:0003824 | catalytic activity |
GO:0004556 | alpha-amylase activity |
GO:0005509 | calcium ion binding |
GO:0005975 | carbohydrate metabolic process |
GO:0043169 | cation binding |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
EMBL | CBI21221.1 | 0 | 1 | 972 | 1 | 975 | unnamed protein product [Vitis vinifera] |
RefSeq | XP_001782830.1 | 0 | 580 | 971 | 14 | 404 | predicted protein [Physcomitrella patens subsp. patens] |
RefSeq | XP_002276872.1 | 0 | 1 | 965 | 1 | 968 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002324108.1 | 0 | 581 | 972 | 10 | 401 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002526120.1 | 0 | 1 | 972 | 1 | 972 | alpha-amylase, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3bsg_A | 0 | 577 | 940 | 1 | 377 | A Chain A, Barley Alpha-Amylase Isozyme 1 (Amy1) H395a Mutant |
PDB | 2qps_A | 0 | 577 | 940 | 1 | 377 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
PDB | 1rpk_A | 0 | 577 | 940 | 1 | 377 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
PDB | 1p6w_A | 0 | 577 | 940 | 1 | 377 | A Chain A, "sugar Tongs" Mutant Y380a In Complex With Acarb |
PDB | 1ht6_A | 0 | 577 | 940 | 1 | 377 | A Chain A, Crystal Structure At 1.5a Resolution Of The Barley Alpha- Amylase Isozyme 1 |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
HO795567 | 638 | 339 | 972 | 0 |
HO778903 | 507 | 98 | 600 | 0 |
HO778903 | 105 | 600 | 704 | 0 |
HO785297 | 258 | 583 | 840 | 0 |
HO785297 | 103 | 860 | 962 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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