Basic Information | |
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Species | Ricinus communis |
Cazyme ID | 30147.m014213 |
Family | GH32 |
Protein Properties | Length: 639 Molecular Weight: 71489.7 Isoelectric Point: 4.4942 |
Chromosome | Chromosome/Scaffold: 30147 Start: 991605 End: 995194 |
Description | Glycosyl hydrolases family 32 protein |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 121 | 437 | 0 |
HFQPEKNWMNGPLFYMGWYHLFYQYNPDSAVWGNITWGHAVSRDLIHWLYLPMAMVPDKWYDINGVWTGSATLLPDGQIVMLYTGDTNASVQVQNLAYPA NLSDPLLIDWIKYPGNPVLVPPPGIETDEFRDPTTAWMGPDGTWRITLGSRMNETVGISLVYQTTNFTTYELLDGLLHAVPGTGMWECVDFYPVAINGSK GLDTSVNGPGVKHVLKASLDNTKLDYYALGTYDPVTDKWTPDNPEEDVGIGLRVDYGRYYASKSFYDQYKQRRILWGWINETDTEQDDLQKGWASVQTIP RNVLFDNKTGANLLQWP |
Full Sequence |
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Protein Sequence Length: 639 Download |
MDCMPSEITE HAPCTPLLEQ PSLLGPSTKL RRPLKVFTVT FASLIFLLSL VTLIMNQSQG 60 PLPTPNKNRS PSTPKSTSFS EHVPRGVAEG VSAKSNPSFF SNGVSYNWTN AMLSWQRTAY 120 HFQPEKNWMN GPLFYMGWYH LFYQYNPDSA VWGNITWGHA VSRDLIHWLY LPMAMVPDKW 180 YDINGVWTGS ATLLPDGQIV MLYTGDTNAS VQVQNLAYPA NLSDPLLIDW IKYPGNPVLV 240 PPPGIETDEF RDPTTAWMGP DGTWRITLGS RMNETVGISL VYQTTNFTTY ELLDGLLHAV 300 PGTGMWECVD FYPVAINGSK GLDTSVNGPG VKHVLKASLD NTKLDYYALG TYDPVTDKWT 360 PDNPEEDVGI GLRVDYGRYY ASKSFYDQYK QRRILWGWIN ETDTEQDDLQ KGWASVQTIP 420 RNVLFDNKTG ANLLQWPVEE IESLRVNSTD FQEIVIAPGS VVPLEIGTAT QLDIFAEFET 480 ELISESSTEE YGCSGGAVDR SSLGPFGLLV LADESLSELT PVFFRPVNST DDTLKTYFCA 540 DETRSSKAPE VFKQVYGSTV PVLDDEKLRM RVLVDHSIVE SFAQGGRTVI TSRIYPTEAI 600 YGAARLFLFN NATDVNVKAT LKIWELTSAF IRPFPFDEN |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
TIGR01322 | scrB_fam | 3.0e-49 | 117 | 596 | 507 | + sucrose-6-phosphate hydrolase. [Energy metabolism, Biosynthesis and degradation of polysaccharides]. | ||
COG1621 | SacC | 2.0e-70 | 117 | 628 | 526 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 1.0e-88 | 127 | 440 | 328 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 2.0e-147 | 121 | 437 | 327 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 0 | 121 | 586 | 478 | + Glycosyl hydrolases family 32. |
Gene Ontology | |
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GO Term | Description |
GO:0004564 | beta-fructofuranosidase activity |
GO:0004575 | sucrose alpha-glucosidase activity |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | ACJ04702.1 | 0 | 26 | 634 | 21 | 629 | invertase 2 [Cucumis melo] |
GenBank | ACQ82802.1 | 0 | 6 | 638 | 4 | 635 | vacuolar invertase [Gossypium hirsutum] |
DDBJ | BAF34363.1 | 0 | 20 | 638 | 22 | 641 | beta-fructofuranosidase [Citrus sinensis] |
RefSeq | XP_002321831.1 | 0 | 112 | 639 | 1 | 528 | predicted protein [Populus trichocarpa] |
RefSeq | XP_002510944.1 | 0 | 1 | 639 | 1 | 639 | Beta-fructofuranosidase, soluble isoenzyme I precursor, putative [Ricinus communis] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 3ugh_B | 0 | 99 | 634 | 1 | 544 | B Chain B, Crystal Structure Of The Complex Between Pectin Methylesterase And Its Inhibitor Protein |
PDB | 3ugh_A | 0 | 99 | 634 | 1 | 544 | B Chain B, Crystal Structure Of The Complex Between Pectin Methylesterase And Its Inhibitor Protein |
PDB | 3ugg_B | 0 | 99 | 634 | 1 | 544 | B Chain B, Crystal Structure Of The Complex Between Pectin Methylesterase And Its Inhibitor Protein |
PDB | 3ugg_A | 0 | 99 | 634 | 1 | 544 | B Chain B, Crystal Structure Of The Complex Between Pectin Methylesterase And Its Inhibitor Protein |
PDB | 3ugf_B | 0 | 99 | 634 | 1 | 544 | A Chain A, Crystal Structure Of A 6-Sst6-Sft From Pachysandra Terminalis |