Basic Information | |
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Species | Selaginella moellendorffii |
Cazyme ID | 31903 |
Family | GH32 |
Protein Properties | Length: 525 Molecular Weight: 57069.4 Isoelectric Point: 5.8415 |
View CDS |
External Links |
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NCBI Taxonomy |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH32 | 3 | 310 | 0 |
HFQAPTNSSIWMISPNAPVFYRGFYHLFYRFTTPSSSKSKNQSSWGHAIAKDLLHWTHLPTALDPGPERYDEQGILGGSMTLLVQGPVILYTGISSDGAT TQNAAVPVDPGDAMLKHWKKIAQNPLIPAGGRVAMRDPSSAWRDSSWRILLGGENASDGVGLVYWSNDFLDGEWKRLETPLLRMPGAGILESPDFFQVSE SKHVVKASLRDDPAATFGSDSYAVGRYFSENGSFVPDDDHGAGRTLGLRYDHGNSFFASKSFADTDKDRRVLWALLPEDPPSKVAARAPPVQSIPRKLWL GSDEDEEE |
Full Sequence |
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Protein Sequence Length: 525 Download |
GFHFQAPTNS SIWMISPNAP VFYRGFYHLF YRFTTPSSSK SKNQSSWGHA IAKDLLHWTH 60 LPTALDPGPE RYDEQGILGG SMTLLVQGPV ILYTGISSDG ATTQNAAVPV DPGDAMLKHW 120 KKIAQNPLIP AGGRVAMRDP SSAWRDSSWR ILLGGENASD GVGLVYWSND FLDGEWKRLE 180 TPLLRMPGAG ILESPDFFQV SESKHVVKAS LRDDPAATFG SDSYAVGRYF SENGSFVPDD 240 DHGAGRTLGL RYDHGNSFFA SKSFADTDKD RRVLWALLPE DPPSKVAARA PPVQSIPRKL 300 WLGSDEDEEE LLLQLPVDEL ASLRIGPGIH MADVTLEPGA VIQVGGDALP QLDAELVFEL 360 PDLTTQMSME NFDVEFSSTG NAAAHCRKKG ARVNGILGPF GLLVLATGDL AEHTAIFFHL 420 GRSRKGPVAL VCADHSRSSL ANHPEEDLSA AALHGAFVAK AEVEVLELRV LVDNSVVETF 480 AQGGKISITS RVYPTLARGD AARLFLFNNG SNAVRLKRLD AWTMK |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
COG1621 | SacC | 2.0e-30 | 2 | 282 | 297 | + Beta-fructosidases (levanase/invertase) [Carbohydrate transport and metabolism] | ||
cd08996 | GH32_B_Fructosidase | 2.0e-44 | 13 | 299 | 299 | + Glycosyl hydrolase family 32, beta-fructosidases. Glycosyl hydrolase family GH32 cleaves sucrose into fructose and glucose via beta-fructofuranosidase activity, producing invert sugar that is a mixture of dextrorotatory D-glucose and levorotatory D-fructose, thus named invertase (EC 3.2.1.26). This family also contains other fructofuranosidases such as inulinase (EC 3.2.1.7), exo-inulinase (EC 3.2.1.80), levanase (EC 3.2.1.65), and transfructosidases such sucrose:sucrose 1-fructosyltransferase (EC 2.4.1.99), fructan:fructan 1-fructosyltransferase (EC 2.4.1.100), sucrose:fructan 6-fructosyltransferase (EC 2.4.1.10), fructan:fructan 6G-fructosyltransferase (EC 2.4.1.243) and levan fructosyltransferases (EC 2.4.1.-). These retaining enzymes (i.e. they retain the configuration at anomeric carbon atom of the substrate) catalyze hydrolysis in two steps involving a covalent glycosyl enzyme intermediate: an aspartate located close to the N-terminus acts as the catalytic nucleophile and a glutamate acts as the general acid/base; a conserved aspartate residue in the Arg-Asp-Pro (RDP) motif stabilizes the transition state. These enzymes are predicted to display a 5-fold beta-propeller fold as found for GH43 and CH68. The breakdown of sucrose is widely used as a carbon or energy source by bacteria, fungi, and plants. Invertase is used commercially in the confectionery industry, since fructose has a sweeter taste than sucrose and a lower tendency to crystallize. A common structural feature of all these enzymes is a 5-bladed beta-propeller domain, similar to GH43, that contains the catalytic acid and catalytic base. A long V-shaped groove, partially enclosed at one end, forms a single extended substrate-binding surface across the face of the propeller. | ||
pfam00251 | Glyco_hydro_32N | 3.0e-72 | 3 | 299 | 315 | + Glycosyl hydrolases family 32 N-terminal domain. This domain corresponds to the N-terminal domain of glycosyl hydrolase family 32 which forms a five bladed beta propeller structure. | ||
smart00640 | Glyco_32 | 7.0e-74 | 3 | 484 | 500 | + Glycosyl hydrolases family 32. |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
GenBank | AAT09980.1 | 0 | 1 | 525 | 52 | 571 | cell wall apoplastic invertase [Vitis vinifera] |
EMBL | CAD19323.1 | 0 | 1 | 525 | 44 | 561 | exocellular acid invertase 2 [Beta vulgaris] |
EMBL | CAD48404.1 | 0 | 1 | 525 | 58 | 580 | fructan 6-exohydrolase [Beta vulgaris] |
RefSeq | XP_002279788.1 | 0 | 1 | 525 | 51 | 570 | PREDICTED: hypothetical protein [Vitis vinifera] |
RefSeq | XP_002309497.1 | 0 | 2 | 525 | 50 | 562 | predicted protein [Populus trichocarpa] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 2aey_A | 0 | 1 | 525 | 10 | 529 | A Chain A, Crystal Structure Of Uncharacterized Conserved Protein From Thermus Thermophilus Hb8 |
PDB | 2ade_A | 0 | 1 | 525 | 10 | 529 | A Chain A, Crystal Structure Of Uncharacterized Conserved Protein From Thermus Thermophilus Hb8 |
PDB | 2add_A | 0 | 1 | 525 | 10 | 529 | A Chain A, Crystal Structure Of Uncharacterized Conserved Protein From Thermus Thermophilus Hb8 |
PDB | 1st8_A | 0 | 1 | 525 | 10 | 529 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia From Cichorium Intybus |
PDB | 2aez_A | 0 | 1 | 525 | 10 | 529 | A Chain A, Crystal Structure Of Fructan 1-Exohydrolase Iia (E201q) From Cichorium Intybus In Complex With 1-Kestose |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
FE508390 | 257 | 125 | 381 | 0 |
FE508391 | 257 | 125 | 381 | 0 |
FE448345 | 213 | 23 | 235 | 0 |
FE487661 | 175 | 61 | 235 | 0 |
FE487661 | 175 | 61 | 235 | 0 |
Sequence Alignments (This image is cropped. Click for full image.) |
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