Basic Information | |
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Species | Ricinus communis |
Cazyme ID | 34575.m000024 |
Family | GH20 |
Protein Properties | Length: 597 Molecular Weight: 65754.7 Isoelectric Point: 7.3557 |
Chromosome | Chromosome/Scaffold: 34575 Start: 15 End: 1808 |
Description | beta-hexosaminidase 2 |
View CDS |
External Links |
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NCBI Taxonomy |
Plaza |
CAZyDB |
Signature Domain Download full data set without filtering | |||
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Family | Start | End | Evalue |
GH20 | 71 | 481 | 0 |
VDAPRFDYRGFQLDVARNFHTRQTVFKWLDLMARYKLNKFHFHLTDDEGWRLEIPGLPELTSIGAVRGHSARPGVRLQPAYGSGPDPKDASGSGYYTRAD YIEILRYAAARHIDVIPEIEMPGHARAAVQSMEARYRRLKAAGRPDAAQYLLNDLADKSVYKSPQLYNDHVINPGLESSYAFIDHVFAQIVAMHREAGAP LRTIHVGGDELPRGAWEKSPASLALMRKQKLDTTADLWDYFYDRVDGMLKKHGLYASGWEELAGRVTTVRGQSTLIPNPLFTRRGFRAYVWNNTEGAEDL AYRLANAGYDVVLAPVTRMYMDMSHNPNPEEPGVNWGAYVELDTVYDFIPLDYMKNASPPLAGKVGLTDSGRRHVRGMEATLFTETVRDPSLIDYLVMPR LLAVAERAWAP |
Full Sequence |
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Protein Sequence Length: 597 Download |
MQGAPVLRLG IGAVPGQTSP EAYTLDINSD GITIVGNSAA GVAYGLQSLR DLLPLPGAAP 60 HGIDLPRLHI VDAPRFDYRG FQLDVARNFH TRQTVFKWLD LMARYKLNKF HFHLTDDEGW 120 RLEIPGLPEL TSIGAVRGHS ARPGVRLQPA YGSGPDPKDA SGSGYYTRAD YIEILRYAAA 180 RHIDVIPEIE MPGHARAAVQ SMEARYRRLK AAGRPDAAQY LLNDLADKSV YKSPQLYNDH 240 VINPGLESSY AFIDHVFAQI VAMHREAGAP LRTIHVGGDE LPRGAWEKSP ASLALMRKQK 300 LDTTADLWDY FYDRVDGMLK KHGLYASGWE ELAGRVTTVR GQSTLIPNPL FTRRGFRAYV 360 WNNTEGAEDL AYRLANAGYD VVLAPVTRMY MDMSHNPNPE EPGVNWGAYV ELDTVYDFIP 420 LDYMKNASPP LAGKVGLTDS GRRHVRGMEA TLFTETVRDP SLIDYLVMPR LLAVAERAWA 480 PDPAWATETD AAQAVVLHRA AWSGFVNVLG QRVLPRLDLE KTGVAYRLAP PGLMLDGGRV 540 LANHVLPGIT LRYTSDGTTP TASSRMVDGA ITDRGVIQVA AFDRNGRAGR VSRIDNP 600 |
Functional Domains Download unfiltered results here | ||||||||
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Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
cd06568 | GH20_SpHex_like | 6.0e-72 | 76 | 484 | 411 | + A subgroup of the Glycosyl hydrolase family 20 (GH20) catalytic domain found in proteins similar to the N-acetylhexosaminidase from Streptomyces plicatus (SpHex). SpHex catalyzes the hydrolysis of N-acetyl-beta-hexosaminides. An Asp residue within the active site plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself. Proteins belonging to this subgroup lack the C-terminal PKD (polycystic kidney disease I)-like domain found in the chitobiases. | ||
COG3525 | Chb | 3.0e-106 | 21 | 565 | 562 | + N-acetyl-beta-hexosaminidase [Carbohydrate transport and metabolism] | ||
pfam00728 | Glyco_hydro_20 | 8.0e-113 | 76 | 482 | 408 | + Glycosyl hydrolase family 20, catalytic domain. This domain has a TIM barrel fold. | ||
cd06563 | GH20_chitobiase-like | 3.0e-115 | 76 | 481 | 408 | + The chitobiase of Serratia marcescens is a beta-N-1,4-acetylhexosaminidase with a glycosyl hydrolase family 20 (GH20) domain that hydrolyzes the beta-1,4-glycosidic linkages in oligomers derived from chitin. Chitin is degraded by a two step process: i) a chitinase hydrolyzes the chitin to oligosaccharides and disaccharides such as di-N-acetyl-D-glucosamine and chitobiose, ii) chitobiase then further degrades these oligomers into monomers. This GH20 domain family includes an N-acetylglucosamidase (GlcNAcase A) from Pseudoalteromonas piscicida and an N-acetylhexosaminidase (SpHex) from Streptomyces plicatus. SpHex lacks the C-terminal PKD (polycystic kidney disease I)-like domain found in the chitobiases. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself. | ||
cd06569 | GH20_Sm-chitobiase-like | 0 | 72 | 509 | 453 | + The chitobiase of Serratia marcescens is a beta-N-1,4-acetylhexosaminidase with a glycosyl hydrolase family 20 (GH20) domain that hydrolyzes the beta-1,4-glycosidic linkages in oligomers derived from chitin. Chitin is degraded by a two step process: i) a chitinase hydrolyzes the chitin to oligosaccharides and disaccharides such as di-N-acetyl-D-glucosamine and chitobiose, ii) chitobiase then further degrades these oligomers into monomers. The GH20 hexosaminidases are thought to act via a catalytic mechanism in which the catalytic nucleophile is not provided by solvent or the enzyme, but by the substrate itself. |
Gene Ontology | |
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GO Term | Description |
GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds |
GO:0004563 | beta-N-acetylhexosaminidase activity |
GO:0005975 | carbohydrate metabolic process |
Annotations - NR Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
DDBJ | BAA92145.1 | 0 | 7 | 594 | 230 | 834 | beta-N-acetylglucosaminidase [Aeromonas sp. 10S-24] |
RefSeq | XP_002536027.1 | 0 | 1 | 597 | 1 | 597 | Beta-hexosaminidase, putative [Ricinus communis] |
RefSeq | YP_003121185.1 | 0 | 16 | 597 | 256 | 853 | Beta-N-acetylhexosaminidase [Chitinophaga pinensis DSM 2588] |
RefSeq | YP_003292053.1 | 0 | 7 | 594 | 248 | 856 | Beta-N-acetylhexosaminidase [Rhodothermus marinus DSM 4252] |
RefSeq | YP_003386134.1 | 0 | 8 | 594 | 255 | 850 | Beta-N-acetylhexosaminidase [Spirosoma linguale DSM 74] |
Annotations - PDB Download unfiltered results here | |||||||
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Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
PDB | 1qbb_A | 0 | 22 | 557 | 261 | 821 | A Chain A, Bacterial Chitobiase Complexed With Chitobiose (Dinag) |
PDB | 1qba_A | 0 | 22 | 557 | 261 | 821 | A Chain A, Bacterial Chitobiase Complexed With Chitobiose (Dinag) |
PDB | 1c7t_A | 0 | 22 | 557 | 261 | 821 | A Chain A, Beta-N-Acetylhexosaminidase Mutant E540d Complexed With Di- N Acetyl-D-Glucosamine (Chitobiase) |
PDB | 1c7s_A | 0 | 22 | 557 | 261 | 821 | A Chain A, Beta-N-Acetylhexosaminidase Mutant D539a Complexed With Di- N-Acetyl-Beta-D-Glucosamine (Chitobiase) |
PDB | 1m01_A | 0 | 21 | 481 | 98 | 476 | A Chain A, Beta-N-Acetylhexosaminidase Mutant D539a Complexed With Di- N-Acetyl-Beta-D-Glucosamine (Chitobiase) |
EST Download unfiltered results here | ||||
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Hit | Length | Start | End | EValue |
CX942806 | 142 | 16 | 155 | 7.00005e-41 |
CV734897 | 346 | 26 | 363 | 3e-23 |
EC096496 | 144 | 21 | 163 | 4e-19 |
FE451689 | 144 | 70 | 212 | 5e-19 |
CX942806 | 54 | 145 | 196 | 0.00000004 |
Sequence Alignments (This image is cropped. Click for full image.) |
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