| Basic Information | |
|---|---|
| Species | Arabidopsis lyrata |
| Cazyme ID | 470978 |
| Family | GT8 |
| Protein Properties | Length: 528 Molecular Weight: 61506 Isoelectric Point: 9.7738 |
| Chromosome | Chromosome/Scaffold: 1 Start: 3416200 End: 3418438 |
| Description | plant glycogenin-like starch initiation protein 5 |
| View CDS | |
| External Links |
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| NCBI Taxonomy |
| Plaza |
| CAZyDB |
| Signature Domain Download full data set without filtering | |||
|---|---|---|---|
| Family | Start | End | Evalue |
| GT8 | 227 | 444 | 5.9e-39 |
| TKTTLSSITAQRVAYVTLLHSSEVYVCGAIALAQSIRQSGSTKDMVLLHDDSITNSSLIGLRLAGWKLRRVERIRSPFSKKRSYNEWNYSKLRVWQVTDY DKLVFIDADFIIVKNIDYLFFYPQLSAAGNNKVMFNSGVMVLEPSACLFEDLMLKSFKIGSYNGGDQGFLNEYFVWWHRLSKRLNTMKYFGDESRHDKAR NLPENLEGIHYLGLKPWR | |||
| Full Sequence |
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| Protein Sequence Length: 528 Download |
| MIKSPSIVEI RYSLPEPVNR HPIWLRLIRN YLPDEKKIRV GLLNIAVNER ESYEASGTSI 60 LKNVHVSLDP LPNNLTWERL FPVWIDEDHT WHTPSCPEVP LPKMEGIDAD VDVVVVKVPC 120 DGFSEKRGLR DVFRLQVNLA AANLAVESGW RNVDRMVYVV FIGSCGPMHE IFRCDERVKR 180 VGDYWVYRPD LTRLKQKLLM PPGSCRIAPP GQEEAWIQDK NKSLTSTKTT LSSITAQRVA 240 YVTLLHSSEV YVCGAIALAQ SIRQSGSTKD MVLLHDDSIT NSSLIGLRLA GWKLRRVERI 300 RSPFSKKRSY NEWNYSKLRV WQVTDYDKLV FIDADFIIVK NIDYLFFYPQ LSAAGNNKVM 360 FNSGVMVLEP SACLFEDLML KSFKIGSYNG GDQGFLNEYF VWWHRLSKRL NTMKYFGDES 420 RHDKARNLPE NLEGIHYLGL KPWRCYRDYD CNWDLKTRRV YASESVHERW WKVYDKMPKK 480 LKGYCGLTLK MEKNVEKWRR MAKLSGFPEN HWKIRVRDPR KKNRLSQ* 540 |
| Functional Domains Download unfiltered results here | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cdd ID | Domain | E-Value | Start | End | Length | Domain Description | ||
| COG5597 | COG5597 | 6.0e-10 | 238 | 356 | 144 | + Alpha-N-acetylglucosamine transferase [Cell envelope biogenesis, outer membrane] | ||
| PLN00176 | PLN00176 | 3.0e-14 | 240 | 475 | 292 | + galactinol synthase | ||
| cd06914 | GT8_GNT1 | 6.0e-15 | 240 | 400 | 174 | + GNT1 is a fungal enzyme that belongs to the GT 8 family. N-acetylglucosaminyltransferase is a fungal enzyme that catalyzes the addition of N-acetyl-D-glucosamine to mannotetraose side chains by an alpha 1-2 linkage during the synthesis of mannan. The N-acetyl-D-glucosamine moiety in mannan plays a role in the attachment of mannan to asparagine residues in proteins. The mannotetraose and its N-acetyl-D-glucosamine derivative side chains of mannan are the principle immunochemical determinants on the cell surface. N-acetylglucosaminyltransferase is a member of glycosyltransferase family 8, which are, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed, retaining glycosyltransferases. | ||
| pfam01501 | Glyco_transf_8 | 3.0e-40 | 242 | 446 | 248 | + Glycosyl transferase family 8. This family includes enzymes that transfer sugar residues to donor molecules. Members of this family are involved in lipopolysaccharide biosynthesis and glycogen synthesis. This family includes Lipopolysaccharide galactosyltransferase, lipopolysaccharide glucosyltransferase 1, and glycogenin glucosyltransferase. | ||
| cd02537 | GT8_Glycogenin | 1.0e-71 | 239 | 477 | 249 | + Glycogenin belongs the GT 8 family and initiates the biosynthesis of glycogen. Glycogenin initiates the biosynthesis of glycogen by incorporating glucose residues through a self-glucosylation reaction at a Tyr residue, and then acts as substrate for chain elongation by glycogen synthase and branching enzyme. It contains a conserved DxD motif and an N-terminal beta-alpha-beta Rossmann-like fold that are common to the nucleotide-binding domains of most glycosyltransferases. The DxD motif is essential for coordination of the catalytic divalent cation, most commonly Mn2+. Glycogenin can be classified as a retaining glycosyltransferase, based on the relative anomeric stereochemistry of the substrate and product in the reaction catalyzed. It is placed in glycosyltransferase family 8 which includes lipopolysaccharide glucose and galactose transferases and galactinol synthases. | ||
| Gene Ontology | |
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| GO Term | Description |
| GO:0016757 | transferase activity, transferring glycosyl groups |
| Annotations - NR Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| GenBank | AAB70408.1 | 0 | 1 | 527 | 40 | 546 | F7G19.14 [Arabidopsis thaliana] |
| EMBL | CAN81983.1 | 0 | 14 | 522 | 38 | 541 | hypothetical protein [Vitis vinifera] |
| RefSeq | NP_172373.3 | 0 | 1 | 527 | 40 | 566 | PGSIP5 (PLANT GLYCOGENIN-LIKE STARCH INITIATION PROTEIN 5); transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | NP_175891.1 | 0 | 2 | 527 | 37 | 557 | PGSIP4 (PLANT GLYCOGENIN-LIKE STARCH INITIATION PROTEIN 4); transferase, transferring glycosyl groups [Arabidopsis thaliana] |
| RefSeq | XP_002280832.1 | 0 | 14 | 522 | 38 | 541 | PREDICTED: hypothetical protein [Vitis vinifera] |
| Annotations - PDB Download unfiltered results here | |||||||
|---|---|---|---|---|---|---|---|
| Source | Hit ID | E-Value | Query Start | Query End | Hit Start | Hit End | Description |
| PDB | 1zcv_A | 1e-20 | 240 | 460 | 25 | 251 | A Chain A, Apo Form Of A Mutant Of Glycogenin In Which Asp159 Is Replaced By Asn |
| PDB | 3usr_A | 2e-20 | 240 | 474 | 25 | 273 | A Chain A, Structure Of Y194f Glycogenin Mutant Truncated At Residue 270 |
| PDB | 3usq_A | 3e-20 | 240 | 460 | 25 | 251 | A Chain A, Structure Of D159sY194F GLYCOGENIN MUTANT TRUNCATED AT RESIDUE 270 |
| PDB | 3u2x_B | 3e-20 | 233 | 460 | 1 | 232 | A Chain A, Structure Of D159sY194F GLYCOGENIN MUTANT TRUNCATED AT RESIDUE 270 |
| PDB | 3u2x_A | 3e-20 | 233 | 460 | 1 | 232 | A Chain A, Structure Of D159sY194F GLYCOGENIN MUTANT TRUNCATED AT RESIDUE 270 |
| Hydropathy |
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| EST Download unfiltered results here | ||||
|---|---|---|---|---|
| Hit | Length | Start | End | EValue |
| EL450640 | 334 | 147 | 477 | 0 |
| ES903404 | 277 | 1 | 274 | 0 |
| EL376918 | 276 | 248 | 520 | 0 |
| EV045011 | 246 | 91 | 333 | 0 |
| DN503302 | 235 | 237 | 469 | 0 |
| Sequence Alignments (This image is cropped. Click for full image.) |
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